##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490999_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24327382
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.680129658012522	33.0	31.0	34.0	30.0	34.0
2	31.92291015942447	34.0	31.0	34.0	30.0	34.0
3	32.01595613535398	34.0	31.0	34.0	30.0	34.0
4	35.38470243119461	37.0	35.0	37.0	33.0	37.0
5	35.16467119232148	37.0	35.0	37.0	32.0	37.0
6	35.07652352398626	37.0	35.0	37.0	32.0	37.0
7	34.87442253342345	37.0	35.0	37.0	32.0	37.0
8	34.766428997579766	37.0	35.0	37.0	32.0	37.0
9	36.32354089724903	39.0	37.0	39.0	32.0	39.0
10-11	36.181596852468545	39.0	37.0	39.0	32.0	39.0
12-13	35.99243967147801	39.0	37.0	39.0	31.5	39.0
14-15	37.171145810099915	40.0	38.0	41.0	31.0	41.0
16-17	37.21004847541754	40.0	38.0	41.0	31.0	41.0
18-19	37.133456140081165	40.0	38.0	41.0	31.0	41.0
20-21	36.98174388431932	40.0	38.0	41.0	31.0	41.0
22-23	36.800113941565925	40.0	38.0	41.0	30.0	41.0
24-25	36.559094172155476	40.0	37.0	41.0	30.0	41.0
26-27	36.21339357436817	40.0	36.5	41.0	29.5	41.0
28-29	35.888838079658555	40.0	35.5	41.0	27.5	41.0
30-31	35.53421048347907	39.5	35.0	41.0	26.5	41.0
32-33	35.16499128430671	39.0	35.0	41.0	25.0	41.0
34-35	34.780061043970946	39.0	35.0	41.0	23.0	41.0
36-37	34.2947604678547	39.0	35.0	40.0	17.5	41.0
38-39	33.800653888692175	38.0	34.0	40.0	11.5	41.0
40-41	33.263596654173476	38.0	33.0	40.0	4.5	41.0
42-43	32.678987488255004	38.0	33.0	40.0	2.0	41.0
44-45	32.129539709616104	38.0	32.0	40.0	2.0	41.0
46-47	31.563046652533345	38.0	31.0	40.0	2.0	41.0
48-49	30.944544258810915	37.0	30.0	40.0	2.0	41.0
50-51	30.370947478031134	37.0	29.0	40.0	2.0	41.0
52-53	29.833010206359237	36.0	27.5	39.5	2.0	40.0
54-55	29.219590809237097	36.0	26.0	39.0	2.0	40.0
56-57	28.568947513546668	35.0	24.0	39.0	2.0	40.0
58-59	27.92559838539141	35.0	22.5	38.5	2.0	40.0
60-61	27.31191932202158	34.0	19.0	38.0	2.0	40.0
62-63	26.65636814105192	34.0	15.0	38.0	2.0	40.0
64-65	25.959585437512345	33.0	7.5	37.0	2.0	39.0
66-67	25.23865445118591	32.5	4.0	36.5	2.0	39.0
68-69	24.496919767198953	32.0	2.0	36.0	2.0	39.0
70-71	23.753823448819936	31.0	2.0	35.0	2.0	38.0
72-73	22.86782017892431	30.5	2.0	35.0	2.0	37.5
74-75	22.001835894219937	29.0	2.0	34.0	2.0	36.5
76-77	21.278357593102292	28.5	2.0	34.0	2.0	36.0
78-79	21.179666825637053	29.0	2.0	34.0	2.0	35.5
80-81	20.608772493480803	28.5	2.0	34.0	2.0	35.0
82-83	19.952801703035696	27.0	2.0	33.5	2.0	35.0
84-85	19.569838279351224	26.5	2.0	33.0	2.0	35.0
86-87	18.941680058298093	25.5	2.0	33.0	2.0	35.0
88-89	18.160709668635945	24.5	2.0	32.5	2.0	34.0
90-91	17.42621902348555	21.5	2.0	32.0	2.0	34.0
92-93	16.612800711560332	18.0	2.0	31.5	2.0	34.0
94-95	15.681397015922222	4.5	2.0	31.0	2.0	34.0
96-97	14.753974595375697	2.0	2.0	31.0	2.0	34.0
98-99	13.830464350829036	2.0	2.0	30.0	2.0	34.0
100	13.255129261340164	2.0	2.0	29.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.029009327342286895
1101	2	-0.0061715346844515295
1101	3	-0.03142750266095362
1101	4	-0.012482447310112832
1101	5	0.03751004927855206
1101	6	0.0022341398068235208
1101	7	0.053538132088931434
1101	8	0.07400270771002226
1101	9	0.08137755575076966
1101	10-11	0.06229404569068464
1101	12-13	0.03759923130598963
1101	14-15	-0.01091085111221446
1101	16-17	-0.034597107503785196
1101	18-19	-0.07586799189978422
1101	20-21	-0.07266065218283302
1101	22-23	-0.09506174477102292
1101	24-25	-0.13641695782618513
1101	26-27	-0.178977007417906
1101	28-29	-0.18468385867592474
1101	30-31	-0.23682405906046
1101	32-33	-0.2774124649160399
1101	34-35	-0.3476622544579442
1101	36-37	-0.42349992574046524
1101	38-39	-0.484097667226429
1101	40-41	-0.5426959885649367
1101	42-43	-0.5534844704871276
1101	44-45	-0.6837211910775878
1101	46-47	-0.7609687225737538
1101	48-49	-0.808320691805946
1101	50-51	-0.8407128988778823
1101	52-53	-0.9798698557296994
1101	54-55	-0.9887929712063865
1101	56-57	-1.1035001422309847
1101	58-59	-1.2663692716911399
1101	60-61	-1.3647984735719696
1101	62-63	-1.4205675043416086
1101	64-65	-1.5543526211405592
1101	66-67	-1.6973536733213201
1101	68-69	-1.8217887500285173
1101	70-71	-1.920642291250715
1101	72-73	-1.915352836155673
1101	74-75	-1.9904663727500278
1101	76-77	-1.8132363489771208
1101	78-79	-1.9132345916589806
1101	80-81	-1.981831617942209
1101	82-83	-1.9396530116868895
1101	84-85	-1.950566223399992
1101	86-87	-2.0886153837109376
1101	88-89	-2.010491207008858
1101	90-91	-2.1368786356181992
1101	92-93	-2.0585098923872778
1101	94-95	-1.9642155723183663
1101	96-97	-1.9995797946737977
1101	98-99	-2.0301682711907745
1101	100	-1.9008479296912029
1102	1	-0.032185603113905614
1102	2	-0.02094807678918187
1102	3	-0.03437687843196713
1102	4	-0.028716314988535885
1102	5	0.028154204158504115
1102	6	0.018919316061698055
1102	7	0.061748713103320085
1102	8	0.06697837472783874
1102	9	0.06378493114916495
1102	10-11	0.04410729761234222
1102	12-13	-0.015575744519615853
1102	14-15	-0.012064733381663473
1102	16-17	0.002590843583462288
1102	18-19	-0.033027040958330645
1102	20-21	-0.1128346531846276
1102	22-23	-0.08723956941280875
1102	24-25	-0.11936792364350879
1102	26-27	-0.10182122315480768
1102	28-29	-0.11556625968475487
1102	30-31	-0.16307146825915453
1102	32-33	-0.18851212385062155
1102	34-35	-0.23747985804287453
1102	36-37	-0.30158198169104367
1102	38-39	-0.46656260005941874
1102	40-41	-0.497025755580232
1102	42-43	-0.4890785522251164
1102	44-45	-0.5505401747258816
1102	46-47	-0.6367376275117458
1102	48-49	-0.6802384092347253
1102	50-51	-0.7582822877562236
1102	52-53	-0.8844729672263156
1102	54-55	-0.8870346234910897
1102	56-57	-1.004052345700977
1102	58-59	-0.9498954675333451
1102	60-61	-1.0664756197376803
1102	62-63	-1.136734289701831
1102	64-65	-1.1963185768025326
1102	66-67	-1.3798469643452442
1102	68-69	-1.3666167804838665
1102	70-71	-1.4490234196288228
1102	72-73	-1.5362277197406229
1102	74-75	-1.5965175472600777
1102	76-77	-1.455019671813723
1102	78-79	-1.4152330414668377
1102	80-81	-1.4561182018810257
1102	82-83	-1.4111410692708581
1102	84-85	-1.3341922677761744
1102	86-87	-1.6205358692811096
1102	88-89	-1.4998047323508459
1102	90-91	-1.5982370401133892
1102	92-93	-1.5011604698716212
1102	94-95	-1.3834760438519282
1102	96-97	-1.4696897104794555
1102	98-99	-1.5306856942071416
1102	100	-1.4272965012581746
1103	1	0.0033136166360350217
1103	2	0.01042048804432838
1103	3	0.029502390365109932
1103	4	0.04685402048708909
1103	5	0.06771600035464331
1103	6	0.08402685048486802
1103	7	0.10243881133554567
1103	8	0.07761822329643309
1103	9	0.10776748015764781
1103	10-11	0.07009557990023296
1103	12-13	0.07108183016942604
1103	14-15	0.1290160405427372
1103	16-17	0.14308370983268048
1103	18-19	0.137165168815379
1103	20-21	0.08606177968061957
1103	22-23	0.10992700051185977
1103	24-25	0.083734763103962
1103	26-27	0.08520375395374202
1103	28-29	0.07667136425709487
1103	30-31	0.06815650585770783
1103	32-33	0.06721455894647477
1103	34-35	0.07730574878479501
1103	36-37	0.049921330076756476
1103	38-39	0.01352746687377504
1103	40-41	0.029347801995072587
1103	42-43	-0.016448631336203334
1103	44-45	-0.006596930523166122
1103	46-47	-0.07473590088082815
1103	48-49	-0.2616206141672279
1103	50-51	-0.19203989767066076
1103	52-53	-0.224333387562627
1103	54-55	-0.2170409125107824
1103	56-57	-0.19786329495349264
1103	58-59	-0.24136121765665308
1103	60-61	-0.25947505290954
1103	62-63	-0.20889868627710584
1103	64-65	-0.320688450949568
1103	66-67	-0.4605387167855781
1103	68-69	-0.46064025528621144
1103	70-71	-0.5253476163259805
1103	72-73	-0.5988868101476577
1103	74-75	-0.691861551933691
1103	76-77	-0.6140591407172451
1103	78-79	-0.5935243250436031
1103	80-81	-0.6350048985823342
1103	82-83	-0.6525972874112682
1103	84-85	-0.5263512674615036
1103	86-87	-0.7073458174117597
1103	88-89	-0.7222232020192152
1103	90-91	-0.7823952980752686
1103	92-93	-0.6754536983484094
1103	94-95	-0.5982331464225012
1103	96-97	-0.613004797219169
1103	98-99	-0.7098499079683922
1103	100	-0.5745374767737701
1104	1	0.0734112835511489
1104	2	0.08251905286340033
1104	3	0.06145741523795323
1104	4	0.06501519381109233
1104	5	0.14207974356163788
1104	6	0.14632971101990933
1104	7	0.17637718585653062
1104	8	0.1669953391927379
1104	9	0.2033410327460885
1104	10-11	0.2198321177515723
1104	12-13	0.21668306175933338
1104	14-15	0.2521082396033165
1104	16-17	0.2559669874918171
1104	18-19	0.20433766579986923
1104	20-21	0.2175154669580408
1104	22-23	0.18681932697028714
1104	24-25	0.19396448445100134
1104	26-27	0.17436857260674543
1104	28-29	0.24570392362631566
1104	30-31	0.2320332648796537
1104	32-33	0.1877806071799455
1104	34-35	0.20941062834774726
1104	36-37	0.2233569129269597
1104	38-39	0.21859833948907692
1104	40-41	0.1541510726970401
1104	42-43	0.13929945224926144
1104	44-45	0.19490521391168159
1104	46-47	0.19022656544276728
1104	48-49	0.1739892797857081
1104	50-51	0.09993271311266483
1104	52-53	0.12716182350098393
1104	54-55	0.07550853715720507
1104	56-57	0.05854939318904684
1104	58-59	0.13338325105974747
1104	60-61	0.10645728965187473
1104	62-63	0.0720635999787298
1104	64-65	0.09650888218366305
1104	66-67	-0.014790912793497313
1104	68-69	-0.024576935997117744
1104	70-71	-0.03152052174558051
1104	72-73	-0.061606924152666664
1104	74-75	-0.15478362962785042
1104	76-77	-0.11063880723064656
1104	78-79	-0.12050462967303588
1104	80-81	-0.06858873324411618
1104	82-83	-0.15140106556367527
1104	84-85	-0.03674328216542122
1104	86-87	-0.1615141953237078
1104	88-89	-0.16669876235844328
1104	90-91	-0.18972637569250494
1104	92-93	-0.10810537218111804
1104	94-95	-0.05221408197383148
1104	96-97	-0.12395676083995966
1104	98-99	-0.17605284119651365
1104	100	-0.11372149788802055
1105	1	0.06355014248393687
1105	2	0.053371099377564235
1105	3	0.03925502771585343
1105	4	0.028114878869367033
1105	5	0.07671388644009625
1105	6	0.06964548736620202
1105	7	0.11448659754569235
1105	8	0.10562878158739863
1105	9	0.1278557542713159
1105	10-11	0.14488699625235313
1105	12-13	0.14194923219816502
1105	14-15	0.16010832563631539
1105	16-17	0.183655445665714
1105	18-19	0.1550887171775912
1105	20-21	0.16706060328061767
1105	22-23	0.17179177077773744
1105	24-25	0.19633160477267353
1105	26-27	0.21597938353790624
1105	28-29	0.19334574343558586
1105	30-31	0.21703624405207478
1105	32-33	0.24182384628534237
1105	34-35	0.2615514910300121
1105	36-37	0.21904038638028567
1105	38-39	0.25863338506655253
1105	40-41	0.25463755291336554
1105	42-43	0.28064774096590384
1105	44-45	0.38294789887257963
1105	46-47	0.37731928929781944
1105	48-49	0.3648504990785355
1105	50-51	0.31289348333861966
1105	52-53	0.31708274958079485
1105	54-55	0.3148230150099387
1105	56-57	0.29119078297382117
1105	58-59	0.3544981328707699
1105	60-61	0.35235496454501103
1105	62-63	0.371013150963563
1105	64-65	0.3815716187702094
1105	66-67	0.32206254575013915
1105	68-69	0.299381409819226
1105	70-71	0.249407259190626
1105	72-73	0.2507261894684376
1105	74-75	0.07245968447342221
1105	76-77	0.14979502580084159
1105	78-79	0.20446181388602014
1105	80-81	0.18991725380648106
1105	82-83	0.2628114798166834
1105	84-85	0.35346773704812406
1105	86-87	0.2992510224398437
1105	88-89	0.288934286120174
1105	90-91	0.18869456391673012
1105	92-93	0.32448027840747784
1105	94-95	0.4313849807475698
1105	96-97	0.3704655844752729
1105	98-99	0.3099708791583815
1105	100	0.3448819734206072
1106	1	-0.07674253647330076
1106	2	-0.026979392860621232
1106	3	-0.05897671184157005
1106	4	-0.042983346537916134
1106	5	-0.029545400984865466
1106	6	-0.0488462757366932
1106	7	0.015433735223957967
1106	8	0.019166595329060954
1106	9	0.0497884111920186
1106	10-11	0.04165539983942068
1106	12-13	0.03418500901661048
1106	14-15	0.05262226628768474
1106	16-17	0.08110377158696025
1106	18-19	0.08028182931596461
1106	20-21	0.08047558484089734
1106	22-23	0.11272225507657652
1106	24-25	0.11019991037657917
1106	26-27	0.1335056719353176
1106	28-29	0.12099377938962164
1106	30-31	0.13914044333247944
1106	32-33	0.12019037414157197
1106	34-35	0.10699471250207182
1106	36-37	0.18760543994577006
1106	38-39	0.16525595278420013
1106	40-41	0.14646489292288578
1106	42-43	0.1643162212783622
1106	44-45	0.2677131962805319
1106	46-47	0.26656099605821026
1106	48-49	0.26294465136172107
1106	50-51	0.2821329670779278
1106	52-53	0.22323385433690746
1106	54-55	0.23489281512135562
1106	56-57	0.30935818231453993
1106	58-59	0.31657703191380193
1106	60-61	0.3136123326724345
1106	62-63	0.3695180671112688
1106	64-65	0.4281625310230268
1106	66-67	0.3333297322720945
1106	68-69	0.3791136808248794
1106	70-71	0.41306480067224527
1106	72-73	0.37248947431627855
1106	74-75	0.2395299100778132
1106	76-77	0.2912926043151778
1106	78-79	0.35273244905788914
1106	80-81	0.42281071229701794
1106	82-83	0.3728980589191302
1106	84-85	0.4369720793025671
1106	86-87	0.40874703010190316
1106	88-89	0.5865809885517308
1106	90-91	0.5331027741447407
1106	92-93	0.5292585648268116
1106	94-95	0.46862203183874485
1106	96-97	0.3207756021942192
1106	98-99	0.2876445886929577
1106	100	0.3879259324887663
1107	1	-0.08959597976704714
1107	2	-0.10568418055655471
1107	3	-0.11359294434029721
1107	4	-0.10345749644477564
1107	5	-0.08150694748771059
1107	6	-0.04161720570316163
1107	7	-0.05123722162945654
1107	8	-0.046963853892812324
1107	9	-0.06309721818583824
1107	10-11	-0.01938663963712628
1107	12-13	0.01747968611110906
1107	14-15	0.005362783832921991
1107	16-17	-0.05487626545668434
1107	18-19	-0.0037948241204546207
1107	20-21	0.00601647825760665
1107	22-23	0.08766664410517677
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2207	80-81	1.3197705348489386
2207	82-83	1.3549522459421226
2207	84-85	1.281982693334509
2207	86-87	1.385444789310089
2207	88-89	1.3308808677122066
2207	90-91	1.3883629950425238
2207	92-93	1.266739016791039
2207	94-95	1.3214775755386619
2207	96-97	1.3147391883259356
2207	98-99	1.407025896350424
2207	100	1.2841462610250112
2208	1	-0.2958091726127954
2208	2	-0.40601647786262873
2208	3	-0.33084815920157595
2208	4	-0.2949056588034935
2208	5	-0.35401953590414337
2208	6	-0.3018062262402381
2208	7	-0.42252319543762695
2208	8	-0.41404985751646706
2208	9	-0.47524545498386317
2208	10-11	-0.43306832110142324
2208	12-13	-0.42854507421734667
2208	14-15	-0.40165708828923385
2208	16-17	-0.4861153036583161
2208	18-19	-0.4875231471370114
2208	20-21	-0.4742125966787114
2208	22-23	-0.47121698758599706
2208	24-25	-0.41898286782818417
2208	26-27	-0.4058148437974438
2208	28-29	-0.4290327768250606
2208	30-31	-0.3752435295500831
2208	32-33	-0.31814669819824104
2208	34-35	-0.32754578542568424
2208	36-37	-0.2832599206228821
2208	38-39	-0.1814130812373307
2208	40-41	-0.10117103912684655
2208	42-43	-0.028308493076629304
2208	44-45	0.05760700128910656
2208	46-47	0.08619472174772724
2208	48-49	0.09406403987683021
2208	50-51	0.245563279144843
2208	52-53	0.34505782624668413
2208	54-55	0.32459131664826657
2208	56-57	0.40751495979220564
2208	58-59	0.5017290710751183
2208	60-61	0.6665024328859772
2208	62-63	0.8089810370776895
2208	64-65	0.9143033901343713
2208	66-67	1.0692379935073433
2208	68-69	0.9894221572851976
2208	70-71	1.0474475712951659
2208	72-73	1.2081888266518241
2208	74-75	1.4131403368377988
2208	76-77	1.4344892959364657
2208	78-79	1.4160660713353757
2208	80-81	1.4637605127511684
2208	82-83	1.455306725855941
2208	84-85	1.3993786717105152
2208	86-87	1.485132999120001
2208	88-89	1.5744933287768532
2208	90-91	1.6639541218068068
2208	92-93	1.6099612062442041
2208	94-95	1.4992506451091465
2208	96-97	1.3688312297745053
2208	98-99	1.2352616164293604
2208	100	0.8392315167151967
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	331683.0
3	292429.0
4	132233.0
5	105530.0
6	102536.0
7	115082.0
8	130066.0
9	142742.0
10	160877.0
11	179235.0
12	212494.0
13	281397.0
14	419044.0
15	471716.0
16	497956.0
17	476727.0
18	463031.0
19	430461.0
20	420132.0
21	434892.0
22	454323.0
23	479396.0
24	505844.0
25	534946.0
26	569436.0
27	611542.0
28	662275.0
29	725027.0
30	800464.0
31	901099.0
32	1051941.0
33	1286877.0
34	1673440.0
35	2352929.0
36	3070875.0
37	2343278.0
38	493506.0
39	9919.0
40	2.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.921682248150972	27.780897187877514	27.375816859947342	22.92160370402417
2	23.767819950023025	26.37366170254385	27.981390562293164	21.87712778513996
3	23.942196155404776	26.342472203429047	26.157005520220544	23.558326120945637
4	24.62285401060589	24.646688859358196	27.14270891647797	23.587748213557944
5	25.61917852102045	24.967323499984033	25.3869984074939	24.026499571501613
6	25.530510905917442	24.928271322841148	25.435734954703303	24.105482816538103
7	25.323630833376477	26.015809871988697	25.055753038590783	23.604806256044043
8	25.399840100390993	25.295663584205375	25.972053337183993	23.33244297821964
9	24.315059917913683	25.92274886649867	25.66315968879429	24.09903152679336
10-11	24.607088485136202	25.167520301037786	25.979098336242163	24.246292877583848
12-13	23.835775207635436	25.152569773957794	26.459708634367257	24.551946384039514
14-15	23.201816134573804	25.432119395502824	27.95928587626332	23.406778593660054
16-17	22.831366346892406	25.37160073393861	28.641151090540585	23.1558818286284
18-19	22.22226222696016	25.303937548410477	30.03118155504031	22.442618669589052
20-21	21.407167604842716	25.180899647056787	31.57117113998844	21.840761608112054
22-23	21.420552464913722	25.125069493398556	32.018796006202535	21.435582035485183
24-25	20.942802140841852	24.632200225216923	33.05749699769752	21.367500636243708
26-27	20.97063950029504	24.64847407405813	33.77318136388088	20.60770506176595
28-29	21.009416890038104	24.703078183236844	33.73576596488211	20.55173896184294
30-31	20.99075199880571	24.629655994205173	33.71842543108578	20.661166575903337
32-33	21.03581907105136	24.7255931306483	33.54985698368902	20.688730814611322
34-35	21.178609427031823	24.767661836506445	33.437632525072544	20.616096211389188
36-37	21.282004115891354	24.878753957733093	32.99023064027756	20.849011286097987
38-39	21.429471837245192	25.15871824527695	32.383874810821034	21.027935106656823
40-41	21.610127573787896	25.38903127694573	31.78926742388386	21.211573725382515
42-43	21.8053706516413	25.575372102810697	31.071693771378094	21.54756347416991
44-45	21.96039605436134	25.90973818294072	30.406114789321826	21.723750973376113
46-47	22.260817105300397	26.10221838231121	29.787817954787194	21.849146557601195
48-49	22.38358806710741	26.297183643027193	29.180399620740637	22.138828669124756
50-51	22.515241455807526	26.56958804497757	28.594221260815182	22.320949238399724
52-53	22.665209936260833	26.74441398879465	28.262807577634764	22.327568497309755
54-55	22.839935702653218	26.72542620716828	28.04235173470798	22.39228635547052
56-57	22.892264654787333	26.883529521020062	27.862092799400084	22.36211302479252
58-59	23.000085273947057	27.049608175632944	27.660849923504983	22.289456626915012
60-61	23.01569488627779	27.176392059625254	27.479390501106277	22.32852255299068
62-63	23.091952064754935	27.40283064780396	27.26607596681852	22.239141320622586
64-65	23.107756893619765	27.60255048327458	27.068744241709847	22.22094838139581
66-67	23.088962796514952	27.820638193790753	26.83084787536779	22.2595511343265
68-69	23.042627325158733	28.078931097402155	26.612110678162576	22.266330899276536
70-71	23.092375658764308	28.300264355450683	26.40722977143849	22.200130214346526
72-73	23.06741496445946	28.42929133803686	26.12393414684545	22.37935955065823
74-75	23.18309317498174	28.591317098335555	25.946901970017272	22.278687756665434
76-77	22.57682759808934	29.22883769761688	25.65875949050635	22.53557521378743
78-79	22.7994337414976	29.198415311094507	25.48077327259206	22.521377674815827
80-81	22.84748814362577	29.302250720369138	25.25655081523499	22.5937103207701
82-83	22.791165106425257	29.449566462740734	25.084700351087974	22.674568079746034
84-85	22.87563671720956	29.499875746203603	24.900450522006736	22.724037014580105
86-87	22.890620391439974	29.596219488056875	24.710323195006563	22.802836925496585
88-89	23.019523464959	29.673899136352045	24.398435426183582	22.908141972505373
90-91	22.933835865984527	29.600487323322845	24.28572318229052	23.179953628402114
92-93	22.959226876800958	29.674996814278607	24.13495537934748	23.23082092957295
94-95	23.027023587435146	29.626536918402547	23.991365553399557	23.355073940762754
96-97	22.774568714652087	29.652266704835473	23.843100720040912	23.730063860471528
98-99	22.64321270968288	29.625277154297503	23.679051140920603	24.052458995099016
100	23.72554763200392	29.538252541579602	23.23722579759918	23.498974028817297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	500.0
1	549.5
2	783.0
3	1180.5
4	1708.5
5	2405.5
6	3465.5
7	5071.0
8	7318.0
9	10039.0
10	13456.0
11	17480.5
12	20729.5
13	23469.5
14	27319.5
15	31462.0
16	43786.0
17	56440.0
18	62194.5
19	68821.0
20	86043.5
21	96427.5
22	96883.5
23	104022.5
24	109558.5
25	114625.0
26	121027.5
27	131096.5
28	146117.0
29	164783.5
30	186507.0
31	218842.5
32	266807.5
33	318328.0
34	364868.0
35	426482.5
36	508988.0
37	600183.5
38	690350.0
39	755382.5
40	806405.0
41	885636.5
42	1008837.5
43	1114950.5
44	1185788.0
45	1225109.0
46	1191762.5
47	1109669.5
48	1046617.5
49	1028018.5
50	988104.0
51	908133.5
52	808708.0
53	698909.0
54	609050.5
55	536920.0
56	461451.5
57	393239.5
58	345374.5
59	305108.5
60	266532.0
61	229098.5
62	194961.5
63	167030.0
64	144057.0
65	124374.0
66	106703.5
67	90999.0
68	76798.5
69	64008.0
70	52785.5
71	43703.0
72	36631.0
73	30977.5
74	26081.0
75	21491.0
76	17755.5
77	14792.5
78	12280.0
79	10184.0
80	8291.5
81	6657.0
82	5259.5
83	3937.0
84	2782.5
85	1949.0
86	1360.0
87	947.5
88	659.5
89	436.5
90	289.5
91	189.0
92	128.0
93	90.0
94	52.0
95	32.0
96	19.0
97	7.5
98	3.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0927884471909061
2	0.013893809042008713
3	0.004850501381529669
4	0.006807144311706044
5	0.11745612413205826
6	0.04991083709706207
7	0.6746513044436923
8	0.7079512296062108
9	0.6813022461685355
10-11	0.6666418112725817
12-13	0.6590289904602148
14-15	0.6197111551090866
16-17	0.6284297258126665
18-19	0.6724007540145504
20-21	0.7172493941189397
22-23	0.6783097334517951
24-25	0.6906106871672422
26-27	0.6942238996370427
28-29	0.7284445979431736
30-31	0.7639169722414028
32-33	0.6714861467625246
34-35	0.6907381155933672
36-37	0.6809836751032232
38-39	0.70572328744622
40-41	0.7108430327603685
42-43	0.7170808597489036
44-45	0.7166163625827062
46-47	0.7320783633849298
48-49	0.6458360377618931
50-51	0.7176583982608569
52-53	0.742424729467396
54-55	0.7202542386188534
56-57	0.7176070158309678
58-59	0.7226774340124228
60-61	0.6981104666338531
62-63	0.7133813247968894
64-65	0.7181393378046187
66-67	0.702093632598855
68-69	0.7485186856522416
70-71	0.7094618730449499
72-73	0.3757001061602107
74-75	0.8843183372547034
76-77	0.7037440362468924
78-79	0.743248903642817
80-81	0.4219484036547788
82-83	1.4387080368943932E-5
84-85	6.47418616602477E-4
86-87	0.0043037923275097995
88-89	0.0025876191692143446
90-91	8.48837741767692E-4
92-93	3.370687400724007E-4
94-95	7.049669380782527E-4
96-97	0.0014859798723923518
98-99	9.413261155680459E-4
100	7.727917455318456E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.4327382E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.2495981180048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.86064062765503	30.86525563180571
2	9.496551345232824	7.0748544223384116
3	2.9938016436473727	3.345537242126606
4	1.3256748109259262	1.97523415768611
5	0.7190254132299283	1.3391703839723579
6	0.4395082199195893	0.9822902736938625
7	0.3159899594941664	0.823934930033762
8	0.23413127206893536	0.6977036633140068
9	0.1648688107975019	0.5527167249960285
>10	1.072305179583092	7.519484048576326
>50	0.11293393976042003	2.929508047894332
>100	0.1825434024977385	16.890257556962577
>500	0.07309276371464879	18.346536988666262
>1k	0.008602986880460866	4.018326486728856
>5k	9.888737774345928E-5	0.25384739533315787
>10k+	2.307372147347338E-4	2.385342045871568
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	114207	0.4694586536274228	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	95733	0.39351953284574553	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48334	0.19868146930072458	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36208	0.14883640171392054	No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30636	0.1259321697665618	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28942	0.11896882286799296	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27438	0.11278648890373819	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.110594391126838E-6	0.0
2	0.0	0.0	0.0	4.110594391126838E-6	0.0
3	0.0	4.110594391126838E-6	0.0	4.110594391126838E-6	0.0
4	0.0	4.110594391126838E-6	0.0	4.110594391126838E-6	0.0
5	0.0	4.110594391126838E-6	0.0	4.110594391126838E-6	0.0
6	0.0	4.110594391126838E-6	0.0	4.110594391126838E-6	0.0
7	8.221188782253676E-6	8.221188782253676E-6	0.0	4.110594391126838E-6	0.0
8	8.221188782253676E-6	8.221188782253676E-6	0.0	8.221188782253676E-6	0.0
9	1.644237756450735E-5	8.221188782253676E-6	0.0	8.221188782253676E-6	0.0
10-11	4.9327132693522054E-5	1.0276485977817095E-5	0.0	8.221188782253676E-6	0.0
12-13	3.144604709212031E-4	1.644237756450735E-5	0.0	8.221188782253676E-6	0.0
14-15	0.0010913628108441755	1.644237756450735E-5	0.0	8.221188782253676E-6	4.110594391126838E-6
16-17	0.002805480671944067	2.055297195563419E-5	0.0	8.221188782253676E-6	1.0276485977817095E-5
18-19	0.00553080475326116	2.055297195563419E-5	0.0	8.221188782253676E-6	1.2331783173380514E-5
20-21	0.009236505596862007	2.055297195563419E-5	0.0	8.221188782253676E-6	1.2331783173380514E-5
22-23	0.013739661752341457	2.055297195563419E-5	0.0	8.221188782253676E-6	1.2331783173380514E-5
24-25	0.019270466505602615	2.4663566346761027E-5	0.0	8.221188782253676E-6	1.2331783173380514E-5
26-27	0.02525960253347442	2.6718863542324447E-5	0.0	1.0276485977817095E-5	1.4387080368943932E-5
28-29	0.03165568740606778	3.494005232457812E-5	0.0	1.2331783173380514E-5	1.644237756450735E-5
30-31	0.037519450305010214	4.110594391126838E-5	0.0	1.2331783173380514E-5	1.644237756450735E-5
32-33	0.04250765659864263	4.7271835497958634E-5	0.0	1.2331783173380514E-5	1.644237756450735E-5
34-35	0.04726978020076307	5.754832147577573E-5	0.0	1.2331783173380514E-5	1.644237756450735E-5
36-37	0.05192091775432309	5.754832147577573E-5	0.0	1.2331783173380514E-5	1.644237756450735E-5
38-39	0.0561568852743793	5.960361867133915E-5	0.0	1.4387080368943932E-5	1.644237756450735E-5
40-41	0.060064005243145355	6.165891586690257E-5	6.165891586690257E-6	2.055297195563419E-5	2.055297195563419E-5
42-43	0.06390741099884895	6.165891586690257E-5	8.221188782253676E-6	2.055297195563419E-5	2.055297195563419E-5
44-45	0.0680056736068024	6.165891586690257E-5	8.221188782253676E-6	2.055297195563419E-5	2.260826915119761E-5
46-47	0.07233618479785453	6.165891586690257E-5	8.221188782253676E-6	2.055297195563419E-5	2.4663566346761027E-5
48-49	0.07688250219444082	6.165891586690257E-5	8.221188782253676E-6	2.055297195563419E-5	2.6718863542324447E-5
50-51	0.08187687437965993	6.782480745359283E-5	8.221188782253676E-6	2.055297195563419E-5	3.082945793345128E-5
52-53	0.08762965123004193	7.399069904028308E-5	8.221188782253676E-6	2.4663566346761027E-5	3.28847551290147E-5
54-55	0.09418604928388924	8.426718501810019E-5	8.221188782253676E-6	2.4663566346761027E-5	3.494005232457812E-5
56-57	0.10196534916909678	8.63224822136636E-5	8.221188782253676E-6	2.8774160737887867E-5	3.699534952014154E-5
58-59	0.11123679481828336	8.63224822136636E-5	1.644237756450735E-5	3.082945793345128E-5	3.699534952014154E-5
60-61	0.12245460691166851	8.837777940922702E-5	1.644237756450735E-5	3.494005232457812E-5	3.699534952014154E-5
62-63	0.13580376219685292	9.865426538704411E-5	1.644237756450735E-5	4.110594391126838E-5	3.699534952014154E-5
64-65	0.15144868444948167	1.0687545416929779E-4	2.055297195563419E-5	4.110594391126838E-5	3.699534952014154E-5
66-67	0.17031836800194938	1.0687545416929779E-4	2.055297195563419E-5	4.110594391126838E-5	3.699534952014154E-5
68-69	0.1928033193214132	1.0687545416929779E-4	2.055297195563419E-5	4.110594391126838E-5	3.699534952014154E-5
70-71	0.2189528655405666	1.0687545416929779E-4	2.4663566346761027E-5	4.110594391126838E-5	3.699534952014154E-5
72-73	0.2494719735974878	1.0687545416929779E-4	2.4663566346761027E-5	4.110594391126838E-5	3.699534952014154E-5
74-75	0.28490529724900115	1.089307513648612E-4	2.4663566346761027E-5	4.7271835497958634E-5	3.699534952014154E-5
76-77	0.32716220758978504	1.1715194014711488E-4	2.6718863542324447E-5	5.754832147577573E-5	3.699534952014154E-5
78-79	0.3748882637679632	1.2331783173380513E-4	2.8774160737887867E-5	6.165891586690257E-5	3.699534952014154E-5
80-81	0.4292385428074422	1.2331783173380513E-4	2.8774160737887867E-5	6.165891586690257E-5	3.699534952014154E-5
82-83	0.4903651367006939	1.2742842612493199E-4	3.082945793345128E-5	6.165891586690257E-5	3.699534952014154E-5
84-85	0.5581693911823311	1.2742842612493199E-4	3.28847551290147E-5	6.371421306246598E-5	3.699534952014154E-5
86-87	0.6325629284729446	1.315390205160588E-4	3.28847551290147E-5	6.782480745359283E-5	3.699534952014154E-5
88	0.6929311177010333	1.315390205160588E-4	3.28847551290147E-5	6.988010464915625E-5	3.699534952014154E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	26285	0.0	57.209087	1
ATGGATC	35055	0.0	52.449158	3
TATCTCG	29815	0.0	49.513954	2
GGATCCG	37450	0.0	47.89882	5
TATGGAT	39515	0.0	46.8094	2
ATATGGA	40005	0.0	46.66848	1
TGGATCC	39625	0.0	46.209972	4
ATCTCGA	34355	0.0	42.32152	3
TCTCGAG	39035	0.0	36.62085	4
GATCCGG	49785	0.0	35.927258	6
CTCGAGG	44955	0.0	31.733624	5
ATCCGGC	58615	0.0	30.049767	7
TCGAGGG	50900	0.0	27.897882	6
TCCGGCG	77410	0.0	22.729113	8
CGAGGGC	65990	0.0	21.347437	7
CCGGCGC	87370	0.0	19.928795	9
AGGGCGC	73845	0.0	18.86816	9
GAGGGCG	78880	0.0	17.727596	8
GCCGGAT	118900	0.0	13.727196	4
CGGATCC	119155	0.0	13.70486	6
>>END_MODULE