FastQCFastQC Report
Wed 25 May 2016
SRR490998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29489887
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT557710.18911906986961327No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT315150.10686714398057884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCCG252250.041.2699745
ATGGATC278950.038.350693
TGGATCC312250.034.079764
TATGGAT321100.033.3507352
ATCTCGA195500.032.7362943
ATATCTC209700.031.9751131
ATATGGA343050.031.8630581
TATCTCG210150.030.9510562
GATCCGG389200.029.4751821
TCTCGAG218550.029.0252234
CTCGAGG257150.024.5205365
ATCCGGC494150.023.1466122
TCGAGGG308400.020.4152156
GCCGGAT1162950.019.7430135
CGGATCC1167500.019.6539867
CCGGATC1176350.019.5420996
CGCCGGA1231650.018.673464
GATCCTT1240050.018.6285539
GCGCCGG1285850.018.04013
GGATCCT1281900.018.0317278
Produced by FastQC (version 0.11.5)