##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490998_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29489887
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.720473903477487	33.0	31.0	34.0	30.0	34.0
2	31.953801484556383	34.0	31.0	34.0	30.0	34.0
3	32.0387603045071	34.0	31.0	34.0	30.0	34.0
4	35.39507133411532	37.0	35.0	37.0	33.0	37.0
5	35.17126800112866	37.0	35.0	37.0	32.0	37.0
6	35.09260893403898	37.0	35.0	37.0	32.0	37.0
7	34.89335306710399	37.0	35.0	37.0	32.0	37.0
8	34.80442668362887	37.0	35.0	37.0	32.0	37.0
9	36.36090585901533	39.0	37.0	39.0	32.0	39.0
10-11	36.2330002824358	39.0	37.0	39.0	32.0	39.0
12-13	36.06227648481664	39.0	37.0	39.0	32.0	39.0
14-15	37.26723849433536	40.0	38.0	41.0	31.5	41.0
16-17	37.28544485436652	40.0	38.0	41.0	32.0	41.0
18-19	37.20424472972718	40.0	38.0	41.0	31.0	41.0
20-21	37.05909547907051	40.0	38.0	41.0	31.0	41.0
22-23	36.86256878502112	40.0	38.0	41.0	30.0	41.0
24-25	36.59797572299955	40.0	37.5	41.0	30.0	41.0
26-27	36.23344136584857	40.0	36.5	41.0	29.5	41.0
28-29	35.92205917913487	40.0	35.5	41.0	27.5	41.0
30-31	35.600569747859666	40.0	35.0	41.0	26.5	41.0
32-33	35.28065485635804	39.5	35.0	41.0	25.0	41.0
34-35	34.8944043766597	39.0	35.0	41.0	23.5	41.0
36-37	34.3680374902759	39.0	35.0	40.5	17.5	41.0
38-39	33.82736181729011	39.0	34.0	40.0	8.5	41.0
40-41	33.267331814462366	38.0	33.0	40.0	2.0	41.0
42-43	32.68005838747364	38.0	33.0	40.0	2.0	41.0
44-45	32.14599920304883	38.0	32.0	40.0	2.0	41.0
46-47	31.619337435914893	38.0	31.0	40.0	2.0	41.0
48-49	31.053888914528564	38.0	30.0	40.0	2.0	41.0
50-51	30.522914007774936	37.0	29.5	40.0	2.0	41.0
52-53	30.006013434368196	36.5	28.0	39.5	2.0	40.5
54-55	29.39156921828829	36.0	26.5	39.0	2.0	40.0
56-57	28.752215055961386	35.5	25.0	39.0	2.0	40.0
58-59	28.120226028672135	35.0	22.5	38.5	2.0	40.0
60-61	27.510661824509533	34.0	21.0	38.0	2.0	40.0
62-63	26.86047349045454	34.0	16.5	38.0	2.0	40.0
64-65	26.170161130152856	33.5	9.5	37.5	2.0	39.5
66-67	25.454508303134563	33.0	4.5	36.5	2.0	39.0
68-69	24.719687006599923	32.0	2.0	36.0	2.0	39.0
70-71	23.979759281546244	31.0	2.0	35.5	2.0	38.0
72-73	23.101980163572684	30.5	2.0	35.0	2.0	37.5
74-75	22.246705692700687	29.5	2.0	34.0	2.0	36.5
76-77	21.51136625243766	29.0	2.0	34.0	2.0	36.0
78-79	21.412118873158107	29.0	2.0	34.0	2.0	36.0
80-81	20.841127451590438	29.0	2.0	34.0	2.0	35.0
82-83	20.190058561431584	27.5	2.0	34.0	2.0	35.0
84-85	19.802355651617113	27.0	2.0	33.5	2.0	35.0
86-87	19.178994446469055	26.0	2.0	33.0	2.0	35.0
88-89	18.4090530763987	25.0	2.0	32.5	2.0	34.5
90-91	17.691031657734058	23.5	2.0	32.0	2.0	34.0
92-93	16.897320494988673	19.0	2.0	32.0	2.0	34.0
94-95	15.984468065272683	10.5	2.0	31.0	2.0	34.0
96-97	15.090837462347686	2.0	2.0	31.0	2.0	34.0
98-99	14.179386072249107	2.0	2.0	30.5	2.0	34.0
100	13.590201006873984	2.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.03729568120565929
1101	2	-0.029551544355118864
1101	3	-0.048934703700176385
1101	4	-0.02106127497097532
1101	5	0.014831083325653083
1101	6	-0.010225226428318024
1101	7	0.0654111488889555
1101	8	0.05895144090314375
1101	9	0.06867959420858938
1101	10-11	0.0375226788743106
1101	12-13	-0.014112256478625795
1101	14-15	-0.04126450397381376
1101	16-17	-0.041146683731390965
1101	18-19	-0.09875377998957902
1101	20-21	-0.06709137293004375
1101	22-23	-0.0869439061000179
1101	24-25	-0.15182286506138354
1101	26-27	-0.19495826132416738
1101	28-29	-0.2390546210022393
1101	30-31	-0.2735488835492177
1101	32-33	-0.3444107535819967
1101	34-35	-0.3918365844845155
1101	36-37	-0.4735565713632255
1101	38-39	-0.5459285922935209
1101	40-41	-0.6285341013886878
1101	42-43	-0.644003925167155
1101	44-45	-0.7570650426168903
1101	46-47	-0.8264169127916041
1101	48-49	-0.8964058225278073
1101	50-51	-0.9628117697770975
1101	52-53	-1.1035843287005207
1101	54-55	-1.140600908136438
1101	56-57	-1.1842966896451586
1101	58-59	-1.3207184580344098
1101	60-61	-1.4390209289319138
1101	62-63	-1.4605699458993904
1101	64-65	-1.605354180977077
1101	66-67	-1.7450836910342389
1101	68-69	-1.8050759073560911
1101	70-71	-1.891698379930844
1101	72-73	-1.9369240571465483
1101	74-75	-1.97268524728727
1101	76-77	-1.7714659613051253
1101	78-79	-1.8632081357916448
1101	80-81	-1.9152234035442781
1101	82-83	-1.8964134187523243
1101	84-85	-1.9382472384180929
1101	86-87	-2.0916110302245166
1101	88-89	-2.0032307404207863
1101	90-91	-2.1212269888686386
1101	92-93	-2.022591954876397
1101	94-95	-1.9587540728674977
1101	96-97	-2.0319395315788604
1101	98-99	-2.0649713108532506
1101	100	-1.9301812315825408
1102	1	-0.05182247868128087
1102	2	-0.04071197262967985
1102	3	-0.04271730013675068
1102	4	-0.05176307164545335
1102	5	0.018231091572509683
1102	6	0.025535990953308385
1102	7	0.04868919201129529
1102	8	0.06237278909106436
1102	9	0.07091986444169862
1102	10-11	0.03673974578222783
1102	12-13	-0.010728508234670642
1102	14-15	-0.0174361621889787
1102	16-17	-0.002845046351673375
1102	18-19	-0.028774828526820784
1102	20-21	-0.10648701093313662
1102	22-23	-0.08805410863222107
1102	24-25	-0.121060364676147
1102	26-27	-0.11240316246517779
1102	28-29	-0.12338813496559453
1102	30-31	-0.17544659260145323
1102	32-33	-0.22248819942468856
1102	34-35	-0.266939881894821
1102	36-37	-0.3667775614990134
1102	38-39	-0.4624224150393772
1102	40-41	-0.5008443263076856
1102	42-43	-0.5094835296905202
1102	44-45	-0.5675160623778908
1102	46-47	-0.6194097875490492
1102	48-49	-0.6617781136491985
1102	50-51	-0.7223767339013385
1102	52-53	-0.818155509456183
1102	54-55	-0.8588875997393082
1102	56-57	-0.9954536201904247
1102	58-59	-0.9440357158489157
1102	60-61	-1.0577067007369187
1102	62-63	-1.1252032630470694
1102	64-65	-1.18565366443568
1102	66-67	-1.335188738967826
1102	68-69	-1.3967366484284192
1102	70-71	-1.469072794687765
1102	72-73	-1.5651161740000177
1102	74-75	-1.6124538989285213
1102	76-77	-1.4994709243450295
1102	78-79	-1.4561389721751752
1102	80-81	-1.50594273825768
1102	82-83	-1.49467188987677
1102	84-85	-1.4241051175678763
1102	86-87	-1.6210391385075518
1102	88-89	-1.516623112890798
1102	90-91	-1.6490855588726792
1102	92-93	-1.5624340512480863
1102	94-95	-1.4643838976668757
1102	96-97	-1.5850254918273325
1102	98-99	-1.6721083310014642
1102	100	-1.5464187143208523
1103	1	0.015145782226429816
1103	2	0.024864567976617735
1103	3	0.034442768329597584
1103	4	0.05320342220173302
1103	5	0.07997325515589893
1103	6	0.08564935355543213
1103	7	0.10126544483104283
1103	8	0.09599578118879037
1103	9	0.12855221107777481
1103	10-11	0.11578566251860423
1103	12-13	0.09983270936622546
1103	14-15	0.15984129279007675
1103	16-17	0.18937218752836316
1103	18-19	0.18042066407256385
1103	20-21	0.1341673048250982
1103	22-23	0.14425731974343847
1103	24-25	0.09164021142955647
1103	26-27	0.0835152443708651
1103	28-29	0.04598867661061945
1103	30-31	0.08055960235761717
1103	32-33	0.093666799075379
1103	34-35	0.09184006826596658
1103	36-37	0.05917434393260379
1103	38-39	0.024274288651241704
1103	40-41	0.03233270797112198
1103	42-43	-0.04252982005099426
1103	44-45	-0.04608321189223119
1103	46-47	-0.12697552439201942
1103	48-49	-0.3398098943062564
1103	50-51	-0.2672656333403616
1103	52-53	-0.2650705427623379
1103	54-55	-0.3151329356233141
1103	56-57	-0.2918958366942235
1103	58-59	-0.34197952916861496
1103	60-61	-0.3850740084924773
1103	62-63	-0.3700103898738867
1103	64-65	-0.44945446697581914
1103	66-67	-0.5641226548431071
1103	68-69	-0.5387936768852803
1103	70-71	-0.6383807715860996
1103	72-73	-0.7752389928122412
1103	74-75	-0.8477993171017779
1103	76-77	-0.7452229454724559
1103	78-79	-0.7364555207816998
1103	80-81	-0.7639991091994673
1103	82-83	-0.774962196223882
1103	84-85	-0.6789600021354971
1103	86-87	-0.7913116832477982
1103	88-89	-0.7665165941709624
1103	90-91	-0.8532083205018566
1103	92-93	-0.7516481560476649
1103	94-95	-0.7277216423538189
1103	96-97	-0.7531497528830933
1103	98-99	-0.7981291046236763
1103	100	-0.6411282559294538
1104	1	0.04637070703949675
1104	2	0.05315567319542325
1104	3	0.035885895734573126
1104	4	0.030537607062385064
1104	5	0.09689710244457217
1104	6	0.11221306422162058
1104	7	0.12547258783880721
1104	8	0.15382362251569504
1104	9	0.17601659564500238
1104	10-11	0.1960949427535823
1104	12-13	0.1662793740135058
1104	14-15	0.19148058307889215
1104	16-17	0.19282559538017097
1104	18-19	0.15896592008287058
1104	20-21	0.14486611563179963
1104	22-23	0.11213109217759154
1104	24-25	0.08753298378196206
1104	26-27	0.06808382679414393
1104	28-29	0.14095452209274129
1104	30-31	0.11311721841432387
1104	32-33	0.07245914931154118
1104	34-35	0.09770933785292613
1104	36-37	0.10006687161974526
1104	38-39	0.04299958093290712
1104	40-41	-0.001976090711195866
1104	42-43	-0.046735262769878716
1104	44-45	0.0017249189321475455
1104	46-47	0.003148086654601201
1104	48-49	-0.046186439518191236
1104	50-51	-0.12991829089661167
1104	52-53	-0.09871655145479963
1104	54-55	-0.11929485652397176
1104	56-57	-0.12368289740174987
1104	58-59	-0.07373880688065881
1104	60-61	-0.11984422145312479
1104	62-63	-0.17873091451022916
1104	64-65	-0.1429711038048147
1104	66-67	-0.26490703003041105
1104	68-69	-0.29255005561420333
1104	70-71	-0.2997942847879074
1104	72-73	-0.30359424029104076
1104	74-75	-0.38321478138003684
1104	76-77	-0.34826688521155447
1104	78-79	-0.37421296320885133
1104	80-81	-0.31729610738747027
1104	82-83	-0.34846190115828435
1104	84-85	-0.23292324328459202
1104	86-87	-0.3548596269078246
1104	88-89	-0.3863331753382049
1104	90-91	-0.36551808749638326
1104	92-93	-0.29268850057400897
1104	94-95	-0.18095307515256387
1104	96-97	-0.2580971177787852
1104	98-99	-0.2768458803337275
1104	100	-0.19974274623674937
1105	1	0.04730391598517869
1105	2	0.055052722536078846
1105	3	0.04212838296616184
1105	4	0.030192215610341577
1105	5	0.08904952579695902
1105	6	0.06937814272376386
1105	7	0.10267891253459283
1105	8	0.12721006354720998
1105	9	0.1271094178030978
1105	10-11	0.1361485074106099
1105	12-13	0.14406168722417334
1105	14-15	0.15540943554948683
1105	16-17	0.17556435212429022
1105	18-19	0.15320886667242917
1105	20-21	0.1676775878835315
1105	22-23	0.1741523237664353
1105	24-25	0.2031880084610762
1105	26-27	0.19924871441554615
1105	28-29	0.17911139425748956
1105	30-31	0.2062254903339138
1105	32-33	0.21360037890915606
1105	34-35	0.2669173735740813
1105	36-37	0.22967833607235377
1105	38-39	0.2404321074550353
1105	40-41	0.26192858432462174
1105	42-43	0.2835230092100076
1105	44-45	0.36697647390778343
1105	46-47	0.356896535547893
1105	48-49	0.37913326253264756
1105	50-51	0.3015125487650998
1105	52-53	0.34409551355845736
1105	54-55	0.31665828428128506
1105	56-57	0.31360062713243053
1105	58-59	0.41007121608949504
1105	60-61	0.39115123154745746
1105	62-63	0.3765798805653766
1105	64-65	0.36664132941087857
1105	66-67	0.3664430396964775
1105	68-69	0.34271245265518147
1105	70-71	0.27089705950803733
1105	72-73	0.28156258695617
1105	74-75	0.11306471236837368
1105	76-77	0.19368493559785804
1105	78-79	0.2396897275482317
1105	80-81	0.2374478050292339
1105	82-83	0.3231396000537927
1105	84-85	0.41998159778513156
1105	86-87	0.3439481294492168
1105	88-89	0.324779527832618
1105	90-91	0.26902514743624195
1105	92-93	0.4070722662789805
1105	94-95	0.49193631198924415
1105	96-97	0.4636580427417396
1105	98-99	0.4211015078503699
1105	100	0.4416520123133285
1106	1	-0.06786195470050416
1106	2	-0.021275784864151603
1106	3	-0.04347731233286822
1106	4	-0.03375539764392954
1106	5	-0.03472028861385468
1106	6	-0.024476130541799535
1106	7	0.02781368302734677
1106	8	0.05282381101104505
1106	9	0.0516093271762017
1106	10-11	0.04616951832650784
1106	12-13	0.04634448850169548
1106	14-15	0.05136826733487254
1106	16-17	0.08047773786373114
1106	18-19	0.09065497260314714
1106	20-21	0.07649343532018094
1106	22-23	0.11711196078265118
1106	24-25	0.13225035909533034
1106	26-27	0.14762559901000571
1106	28-29	0.13597646191711021
1106	30-31	0.1602929662596395
1106	32-33	0.12929390833474486
1106	34-35	0.1026542283089853
1106	36-37	0.17681349930051482
1106	38-39	0.17639955347684122
1106	40-41	0.17085636165640494
1106	42-43	0.1795174210962429
1106	44-45	0.3108121677264677
1106	46-47	0.30749266508005135
1106	48-49	0.3202789626450482
1106	50-51	0.3335402032162058
1106	52-53	0.30451829601193836
1106	54-55	0.30816294350240625
1106	56-57	0.3368214512936305
1106	58-59	0.32102877292432197
1106	60-61	0.33679062513456515
1106	62-63	0.41631051936750296
1106	64-65	0.46710138192885253
1106	66-67	0.3575656610984659
1106	68-69	0.4013683643145285
1106	70-71	0.3925937211363042
1106	72-73	0.40550587690545115
1106	74-75	0.20992744274252928
1106	76-77	0.3080958626414656
1106	78-79	0.3820479950015887
1106	80-81	0.43219764214092393
1106	82-83	0.41418318824943867
1106	84-85	0.4523924571470346
1106	86-87	0.42631976351452394
1106	88-89	0.5840632268163333
1106	90-91	0.5222124468565212
1106	92-93	0.5166478847092648
1106	94-95	0.46585197686712654
1106	96-97	0.2837126179169971
1106	98-99	0.3016071506664062
1106	100	0.40249731574924574
1107	1	-0.06295766047665552
1107	2	-0.08993651044220385
1107	3	-0.09318618182734184
1107	4	-0.0921733586915181
1107	5	-0.06906880687756001
1107	6	-0.02927088008093648
1107	7	-0.022092937463206397
1107	8	-0.00888775173068268
1107	9	-0.05390802940869577
1107	10-11	0.008929834906979295
1107	12-13	0.022745899748215948
1107	14-15	0.02639973775675486
1107	16-17	-0.026396340555834286
1107	18-19	0.01220247931487961
1107	20-21	0.03470593408336242
1107	22-23	0.10620830596729292
1107	24-25	0.06467118244314207
1107	26-27	0.1108251743765507
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2207	80-81	1.3311035313676172
2207	82-83	1.3497211412903276
2207	84-85	1.2839225578471627
2207	86-87	1.3781075543440515
2207	88-89	1.329373266422671
2207	90-91	1.4187450746817838
2207	92-93	1.2971768174871983
2207	94-95	1.3419684171004445
2207	96-97	1.302667974522997
2207	98-99	1.4127396049257293
2207	100	1.331268464287124
2208	1	-0.31684107400881345
2208	2	-0.42243870005711415
2208	3	-0.3431280079501562
2208	4	-0.2888486272089139
2208	5	-0.3686934216560118
2208	6	-0.3264842390512541
2208	7	-0.44763603547730213
2208	8	-0.42869615600324806
2208	9	-0.5028857196567813
2208	10-11	-0.4550449266507428
2208	12-13	-0.4234550097642398
2208	14-15	-0.4207856027187873
2208	16-17	-0.5234877576204511
2208	18-19	-0.5259016181577891
2208	20-21	-0.5061527477969037
2208	22-23	-0.520311745370158
2208	24-25	-0.43997775267607864
2208	26-27	-0.392322647965095
2208	28-29	-0.45484156394032027
2208	30-31	-0.35154358548533793
2208	32-33	-0.29685812151767266
2208	34-35	-0.2876121843936943
2208	36-37	-0.21761664757202936
2208	38-39	-0.1500320263700985
2208	40-41	-0.0139167300403642
2208	42-43	0.05569136266210961
2208	44-45	0.13043560898329787
2208	46-47	0.1659985611922714
2208	48-49	0.19560586769004829
2208	50-51	0.34740496697901335
2208	52-53	0.43427199538858474
2208	54-55	0.43856789773682436
2208	56-57	0.49293976164384645
2208	58-59	0.5789560087083352
2208	60-61	0.7290204438196959
2208	62-63	0.8678726524436478
2208	64-65	0.9543581116502295
2208	66-67	1.1417513681208362
2208	68-69	1.0690582703270515
2208	70-71	1.1170956438444932
2208	72-73	1.2865753686659396
2208	74-75	1.480857447820032
2208	76-77	1.4961054294994156
2208	78-79	1.4874377833507317
2208	80-81	1.531368375265231
2208	82-83	1.4962649353620634
2208	84-85	1.4682990254411514
2208	86-87	1.5352475494193278
2208	88-89	1.59899911018546
2208	90-91	1.6899181813796247
2208	92-93	1.602676133120859
2208	94-95	1.5330263298318147
2208	96-97	1.4396358346807325
2208	98-99	1.295844187318906
2208	100	0.8734104380867809
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	420068.0
3	351926.0
4	154611.0
5	128658.0
6	124005.0
7	134949.0
8	145506.0
9	158383.0
10	186616.0
11	214245.0
12	262305.0
13	365529.0
14	570641.0
15	615381.0
16	612621.0
17	552829.0
18	524386.0
19	493708.0
20	488105.0
21	503239.0
22	523143.0
23	550111.0
24	576500.0
25	605709.0
26	643984.0
27	688887.0
28	746513.0
29	825781.0
30	932471.0
31	1070093.0
32	1257562.0
33	1548118.0
34	2023005.0
35	2869400.0
36	3829610.0
37	3083678.0
38	694961.0
39	12646.0
40	4.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.628458078783254	27.24132286177338	26.91889360757826	23.211325451865108
2	24.296171902550316	26.548318713990742	26.40257409137178	22.75293529208716
3	24.546769174142106	25.68505887270918	25.87117580370702	23.89699614944169
4	24.69920449834725	24.83955063733459	26.061007993540926	24.400236870777235
5	25.595748378531034	25.074704819332943	25.457455725488643	23.87209107664738
6	24.930853388759868	25.142078673530456	25.09256840046262	24.834499537247055
7	25.231989834114405	25.646373529860494	25.26786348607436	23.853773149950737
8	25.134086977071718	25.656354437953482	25.727777789161436	23.481780795813364
9	24.25400516621186	25.91495272750216	25.980569995861323	23.850472110424654
10-11	23.805954653989886	25.68591786485806	26.340299810771683	24.16782767038037
12-13	23.269110161133742	25.536198681514005	26.68617166355418	24.508519493798072
14-15	22.745205445611866	25.6067833999442	28.50054393404946	23.147467220394475
16-17	22.565362176617253	25.578137148769482	28.972387374799347	22.884113299813926
18-19	22.041251736938463	25.35853770789344	30.06734423233503	22.532866322833062
20-21	21.690595675914953	25.184976771824317	31.096063909327164	22.028363642933563
22-23	21.77523506149871	25.217084071354112	31.286155322275278	21.721525544871902
24-25	21.522665241559125	24.942474337675744	31.763420516232266	21.771439904532862
26-27	21.572592365623155	24.972216715712495	32.04729303747387	21.407897881190475
28-29	21.614452880953845	25.021161865715953	32.010830940300046	21.353554313030155
30-31	21.590047747448505	24.979251419153726	31.96943284456013	21.46126798883764
32-33	21.64272331393135	25.041062134164484	31.838198941328876	21.478015610575298
34-35	21.79409828600758	25.105458478179433	31.681637924167276	21.418805311645713
36-37	21.936514687833604	25.22574120630294	31.149285441056996	21.68845866480646
38-39	22.155560285656026	25.529003929971307	30.390892490162607	21.92454329421006
40-41	22.335382447072558	25.808164150948006	29.714406640261664	22.142046761717772
42-43	22.55642893134415	25.974457681552465	28.990007695950677	22.479105691152707
44-45	22.73315079044266	26.230623758869747	28.368432649567943	22.66779280111965
46-47	23.007717555138623	26.368619965776613	27.911164298682806	22.71249818040196
48-49	23.08903876232374	26.466568196503694	27.49722674039159	22.94716630078098
50-51	23.177737601591907	26.62866093369051	27.12140333999093	23.07219812472665
52-53	23.283989757538283	26.683480080470577	26.99015985018754	23.042370311803598
54-55	23.450382329101537	26.560012505906972	26.8621850827782	23.12742008221329
56-57	23.468717397982175	26.597339630945417	26.838670982169415	23.095271988902994
58-59	23.559038155288487	26.647870355697584	26.780656382371564	23.01243510664236
60-61	23.586654338819486	26.62345295582776	26.715257889483286	23.074634815869473
62-63	23.666212959619163	26.68330904280472	26.648191543562742	23.002286454013372
64-65	23.72180333579009	26.733316596736646	26.57329316982745	22.971586897645818
66-67	23.695224615215658	26.792552718854473	26.45995297154245	23.052269694387423
68-69	23.66237393798879	26.92543444517478	26.345264307463783	23.06692730937265
70-71	23.72259929455336	27.029651496737316	26.221870935906455	23.02587827280287
72-73	23.706763192898354	27.045220163661966	26.04738475368545	23.20063188975423
74-75	23.838709979810076	27.078863381343616	25.972138222040154	23.110288416806153
76-77	23.260178467016264	27.54625849462004	25.769603235958567	23.42395980240513
78-79	23.47399259450519	27.461416932036038	25.6497350451346	23.41485542832417
80-81	23.527326992376352	27.511681270319055	25.46997423754237	23.491017499762222
82-83	23.467456117109187	27.604653141790347	25.369552604000802	23.558338137099668
84-85	23.56809340025591	27.564690776727442	25.22204439680731	23.645171426209334
86-87	23.553750551441222	27.654611503407228	25.08725649137327	23.704381453778282
88-89	23.676502287360286	27.710737146867046	24.83453263709245	23.778227928680213
90-91	23.588428163295553	27.677399053470214	24.731751651150667	24.00242113208356
92-93	23.58159210144649	27.786335552341328	24.60716864576083	24.02490370045135
94-95	23.645983928651088	27.76970608869217	24.469712746980843	24.114597235675898
96-97	23.32875432527888	27.923689634575215	24.368818591336748	24.378737448809158
98-99	23.164486698877223	27.99090238991142	24.22239533843619	24.622215572775165
100	24.21795877713732	27.788307305250214	23.80026516504588	24.193468752566584
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	817.0
1	706.0
2	731.0
3	1014.0
4	1519.5
5	2216.5
6	3361.5
7	5200.0
8	7864.0
9	11409.5
10	15445.5
11	19341.0
12	22266.5
13	24380.0
14	26467.5
15	28458.0
16	33882.5
17	38454.5
18	39335.5
19	41599.5
20	51648.5
21	55723.0
22	54178.0
23	62044.0
24	73064.5
25	86845.5
26	104765.0
27	127467.0
28	154949.0
29	185281.0
30	217246.5
31	260026.5
32	319621.5
33	382997.5
34	439990.5
35	511796.0
36	605922.0
37	712588.5
38	818158.5
39	895866.0
40	959632.5
41	1056163.5
42	1198141.5
43	1313027.0
44	1383329.5
45	1423530.5
46	1394045.0
47	1311830.0
48	1246931.0
49	1224160.0
50	1181908.0
51	1105353.0
52	1006544.0
53	890434.5
54	795482.0
55	716509.5
56	628081.0
57	548411.5
58	491271.5
59	441540.5
60	391820.5
61	342257.5
62	296203.5
63	257837.5
64	226257.0
65	197606.0
66	170175.0
67	145693.0
68	123507.5
69	103543.0
70	85876.0
71	71066.0
72	59396.0
73	49832.0
74	41472.0
75	34166.0
76	27885.0
77	22728.0
78	18730.5
79	15187.0
80	12188.5
81	9677.0
82	7447.5
83	5576.0
84	4065.5
85	2815.5
86	1972.0
87	1410.5
88	976.5
89	681.0
90	482.0
91	313.5
92	193.5
93	121.5
94	69.5
95	40.5
96	27.5
97	16.5
98	5.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09362531636692946
2	0.013784386491545389
3	0.004869465929116649
4	0.007049874419661222
5	0.1185491148202772
6	0.05152952942817312
7	0.6720744640357557
8	0.705825017233874
9	0.6793549259785228
10-11	0.6643277405572968
12-13	0.6566098405192261
14-15	0.6175201688633124
16-17	0.6259043990232991
18-19	0.6691327776196634
20-21	0.7136938164598596
22-23	0.6747686079638081
24-25	0.6874916136504694
26-27	0.6912284879219781
28-29	0.7248535743795831
30-31	0.7596841588440132
32-33	0.6703755765493438
34-35	0.6875848659576077
36-37	0.6779646188539142
38-39	0.7018541644462727
40-41	0.707671412915214
42-43	0.7127985943113312
44-45	0.7133818451050694
46-47	0.7286480955318683
48-49	0.6442191521452761
50-51	0.7148060621595463
52-53	0.7386345698781416
54-55	0.7152384137653698
56-57	0.7142821537430781
58-59	0.7200214093733218
60-61	0.6950467460251712
62-63	0.7107962129525963
64-65	0.7146059935733222
66-67	0.6997008838996229
68-69	0.7440567676641148
70-71	0.7063302751889148
72-73	0.37756841862432367
74-75	0.883194296404052
76-77	0.701735479691733
78-79	0.7396060893688742
80-81	0.4214597363496171
82-83	1.865046142767519E-5
84-85	9.799969731996599E-4
86-87	0.004238741233562542
88-89	0.002589023145460001
90-91	0.001276708859549038
92-93	4.5100206725105454E-4
94-95	0.0011105502031933862
96-97	0.0020464642675639957
98-99	0.0013021413069504132
100	0.0011156366926736614
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.9489887E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.74440785341411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.75802584697325	32.49693534692223
2	11.035073373801476	8.992350604690357
3	3.6541395075418066	4.466572513456715
4	1.6782398999599502	2.7351556383936444
5	0.9320384097113591	1.8987676550163548
6	0.5512615810283603	1.347649601480244
7	0.38191992238828276	1.0892770759592718
8	0.2639944565055969	0.8605038245523546
9	0.21153279690066715	0.7756900696164777
>10	1.2326938312087432	9.616396162352006
>50	0.09783253269906296	2.7679847929422303
>100	0.14648285674498368	14.111924286111726
>500	0.048162087695819276	13.350357456287007
>1k	0.00838693823292871	4.4533216232932045
>5k	1.5781590560713475E-4	0.44738713947051323
>10k+	5.814270206259136E-5	0.5897262094557687
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	55771	0.18911906986961327	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	31515	0.10686714398057884	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.781985973700069E-6	3.3909929868500344E-6	0.0	3.3909929868500344E-6	0.0
2	6.781985973700069E-6	6.781985973700069E-6	0.0	3.3909929868500344E-6	0.0
3	6.781985973700069E-6	6.781985973700069E-6	0.0	3.3909929868500344E-6	0.0
4	6.781985973700069E-6	1.0172978960550103E-5	0.0	6.781985973700069E-6	0.0
5	6.781985973700069E-6	1.0172978960550103E-5	0.0	6.781985973700069E-6	3.3909929868500344E-6
6	6.781985973700069E-6	1.3563971947400138E-5	0.0	6.781985973700069E-6	6.781985973700069E-6
7	6.781985973700069E-6	1.3563971947400138E-5	0.0	1.0172978960550103E-5	6.781985973700069E-6
8	1.0172978960550103E-5	1.3563971947400138E-5	0.0	1.0172978960550103E-5	6.781985973700069E-6
9	2.0345957921100206E-5	1.6954964934250172E-5	0.0	1.0172978960550103E-5	1.0172978960550103E-5
10-11	1.2377124402002625E-4	3.221443337507533E-5	0.0	1.3563971947400138E-5	1.0172978960550103E-5
12-13	5.459498708828555E-4	4.4082908829050444E-5	0.0	1.3563971947400138E-5	1.865046142767519E-5
14-15	0.0017548388706948928	5.764688077645059E-5	0.0	1.3563971947400138E-5	2.3736950907950242E-5
16-17	0.004347253009141744	6.103787376330062E-5	0.0	1.3563971947400138E-5	2.7127943894800275E-5
18-19	0.00840457611790781	6.103787376330062E-5	0.0	2.0345957921100206E-5	2.7127943894800275E-5
20-21	0.014108226321789569	6.612436324357567E-5	0.0	2.3736950907950242E-5	2.7127943894800275E-5
22-23	0.020705403177706312	7.460184571070076E-5	0.0	2.8823440388225295E-5	2.8823440388225295E-5
24-25	0.028433476194737538	8.477482467125086E-5	0.0	3.051893688165031E-5	3.560542636192536E-5
26-27	0.036678675642263396	8.986131415152592E-5	0.0	3.051893688165031E-5	3.730092285535038E-5
28-29	0.045186677046270135	9.155681064495093E-5	0.0	3.221443337507533E-5	3.8996419348775394E-5
30-31	0.05344713596223682	9.494780363180097E-5	0.0	3.8996419348775394E-5	4.069191584220041E-5
32-33	0.06054448428371394	9.8338796618651E-5	0.0	4.577840532247546E-5	4.069191584220041E-5
34-35	0.06742820004701952	1.0172978960550103E-4	0.0	4.91693983093255E-5	4.238741233562543E-5
36-37	0.0738999101624228	1.0681627908577608E-4	0.0	5.595138428302557E-5	4.4082908829050444E-5
38-39	0.07988840377719995	1.1359826505947615E-4	0.0	6.103787376330062E-5	4.4082908829050444E-5
40-41	0.08551406114238416	1.2038025103317622E-4	0.0	6.781985973700069E-5	4.4082908829050444E-5
42-43	0.09024449635903997	1.271622370068763E-4	0.0	7.121085272385072E-5	4.4082908829050444E-5
44-45	0.09506818388283414	1.3563971947400138E-4	0.0	7.121085272385072E-5	4.4082908829050444E-5
46-47	0.10003768410506286	1.3563971947400138E-4	0.0	7.121085272385072E-5	4.4082908829050444E-5
48-49	0.10533611064701603	1.390307124608514E-4	0.0	7.121085272385072E-5	4.7473901815900484E-5
50-51	0.11096007251570683	1.4411720194112645E-4	3.3909929868500344E-6	7.290634921727574E-5	4.7473901815900484E-5
52-53	0.1172622329817676	1.5429018090167657E-4	3.3909929868500344E-6	7.799283869755079E-5	4.91693983093255E-5
54-55	0.12407643338884275	1.6276766336880164E-4	3.3909929868500344E-6	8.138383168440082E-5	5.5951384283025574E-5
56-57	0.1319214956639203	1.7294064232935174E-4	5.086489480275051E-6	8.138383168440082E-5	6.273337025672564E-5
58-59	0.1412738543216527	1.797226283030518E-4	8.477482467125086E-6	8.307932817782584E-5	6.612436324357567E-5
60-61	0.1523946158220274	1.797226283030518E-4	1.0172978960550103E-5	8.986131415152592E-5	6.781985973700069E-5
62-63	0.1655771010584069	1.8141812479647684E-4	1.0172978960550103E-5	9.8338796618651E-5	6.781985973700069E-5
64-65	0.18144694823686508	1.848091177833269E-4	1.0172978960550103E-5	1.0172978960550103E-4	6.781985973700069E-5
66-67	0.1998227392326054	1.96677593237302E-4	1.0172978960550103E-5	1.0681627908577608E-4	7.290634921727574E-5
68-69	0.2222066839388025	2.1532805466497717E-4	1.0172978960550103E-5	1.2207574752660125E-4	7.460184571070076E-5
70-71	0.24896670509452953	2.2380553713210227E-4	1.0172978960550103E-5	1.288577335003013E-4	8.138383168440082E-5
72-73	0.2809946338553281	2.271965301189523E-4	1.0172978960550103E-5	1.3055322999372632E-4	8.477482467125086E-5
74-75	0.31812261606834913	2.3228301959922736E-4	1.0172978960550103E-5	1.3224872648715135E-4	8.647032116467587E-5
76-77	0.36200206531818857	2.373695090795024E-4	1.0172978960550103E-5	1.3563971947400138E-4	8.816581765810089E-5
78-79	0.4130839836720975	2.441514950532025E-4	1.0172978960550103E-5	1.4242170544770145E-4	8.816581765810089E-5
80-81	0.4709529744891867	2.458469915466275E-4	1.0172978960550103E-5	1.492036914214015E-4	8.816581765810089E-5
82-83	0.5369756079431569	2.4923798453347756E-4	1.0172978960550103E-5	1.5429018090167657E-4	8.816581765810089E-5
84-85	0.611953853875398	2.577154670006026E-4	1.3563971947400138E-5	1.5768117388852658E-4	8.986131415152592E-5
86-87	0.6949619712005	2.644974529743027E-4	1.6954964934250172E-5	1.593766703819516E-4	9.155681064495093E-5
88	0.7617085816571627	2.678884459611527E-4	1.6954964934250172E-5	1.593766703819516E-4	9.155681064495093E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATCCG	25225	0.0	41.269974	5
ATGGATC	27895	0.0	38.35069	3
TGGATCC	31225	0.0	34.07976	4
TATGGAT	32110	0.0	33.350735	2
ATCTCGA	19550	0.0	32.736294	3
ATATCTC	20970	0.0	31.975113	1
ATATGGA	34305	0.0	31.863058	1
TATCTCG	21015	0.0	30.951056	2
GATCCGG	38920	0.0	29.475182	1
TCTCGAG	21855	0.0	29.025223	4
CTCGAGG	25715	0.0	24.520536	5
ATCCGGC	49415	0.0	23.146612	2
TCGAGGG	30840	0.0	20.415215	6
GCCGGAT	116295	0.0	19.743013	5
CGGATCC	116750	0.0	19.653986	7
CCGGATC	117635	0.0	19.542099	6
CGCCGGA	123165	0.0	18.67346	4
GATCCTT	124005	0.0	18.628553	9
GCGCCGG	128585	0.0	18.0401	3
GGATCCT	128190	0.0	18.031727	8
>>END_MODULE