FastQCFastQC Report
Wed 25 May 2016
SRR490997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33894431
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1263880.37288721560187865No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1237830.3652015872459992No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT597550.17629739823630614No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT533650.15744474365125055No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA406700.11999021314150397No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396400.11695136584532133No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341530.10076286573449189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC362950.057.37071
TATCTCG389950.052.1290552
ATGGATC463550.045.6866153
ATCTCGA460300.043.4276163
ATATGGA505700.042.3094371
GGATCCG487950.042.17515
TATGGAT518800.041.1376882
TGGATCC516450.040.8206944
TCTCGAG513300.038.2898144
CTCGAGG599350.032.6357465
GATCCGG690100.029.7691176
TCGAGGG678000.028.6659056
ATCCGGC814100.024.6514517
CGAGGGC880450.021.7751167
TCCGGCG1014450.019.6717978
AGGGCGC1041450.018.1835549
CCGGCGC1165050.017.0484459
GAGGGCG1130000.016.8333118
GCGCCGT1204150.014.6231079
CGGCGCG1205050.014.5677775