##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490997_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33894431
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.329230781304457	33.0	31.0	34.0	28.0	34.0
2	31.59689805679287	34.0	31.0	34.0	28.0	34.0
3	31.693370601205846	34.0	31.0	34.0	28.0	34.0
4	35.10109772900451	37.0	35.0	37.0	32.0	37.0
5	34.81385800516905	37.0	35.0	37.0	32.0	37.0
6	34.71428324021725	37.0	35.0	37.0	32.0	37.0
7	34.62451725476672	37.0	35.0	37.0	32.0	37.0
8	34.51600158149874	37.0	35.0	37.0	31.0	37.0
9	36.011021427089304	39.0	37.0	39.0	31.0	39.0
10-11	35.857243583761594	39.0	37.0	39.0	30.5	39.0
12-13	35.647465110123846	39.0	37.0	39.0	30.0	39.0
14-15	36.74468344076937	40.0	37.0	41.0	30.0	41.0
16-17	36.761178938215544	40.0	37.0	41.0	30.0	41.0
18-19	36.65819741892112	40.0	37.0	41.0	30.0	41.0
20-21	36.490608766968236	40.0	37.0	41.0	29.5	41.0
22-23	36.299022839474716	40.0	37.0	41.0	28.5	41.0
24-25	36.078999305225096	40.0	36.5	41.0	27.0	41.0
26-27	35.74908060855189	40.0	35.5	41.0	27.0	41.0
28-29	35.39042496391221	39.0	35.0	41.0	25.5	41.0
30-31	35.0146739297674	39.0	35.0	41.0	24.5	41.0
32-33	34.635600845460424	39.0	35.0	40.0	23.0	41.0
34-35	34.28558007066117	38.5	34.0	40.0	19.5	41.0
36-37	33.81848352904936	38.0	33.5	40.0	15.5	41.0
38-39	33.323692246080185	38.0	33.0	40.0	8.5	41.0
40-41	32.81865265712825	38.0	33.0	40.0	3.5	41.0
42-43	32.243735423674764	38.0	32.0	40.0	2.0	41.0
44-45	31.67833897255865	38.0	31.0	40.0	2.0	41.0
46-47	31.099829334795444	37.0	30.0	40.0	2.0	41.0
48-49	30.48504087588902	37.0	29.5	40.0	2.0	41.0
50-51	29.894513364157078	36.0	27.5	39.5	2.0	40.0
52-53	29.31112175920581	36.0	26.5	39.0	2.0	40.0
54-55	28.660598108285104	35.0	24.0	39.0	2.0	40.0
56-57	27.98127372310808	35.0	22.5	38.5	2.0	40.0
58-59	27.40629932687172	34.0	20.0	38.0	2.0	40.0
60-61	26.702459911482215	34.0	15.5	38.0	2.0	40.0
62-63	25.979268423181377	33.0	7.5	37.5	2.0	39.5
64-65	25.30403268902788	32.5	4.0	37.0	2.0	39.0
66-67	24.536802520744487	31.5	2.0	36.0	2.0	39.0
68-69	23.751857126617644	31.0	2.0	35.5	2.0	38.5
70-71	22.961453328424366	30.5	2.0	35.0	2.0	38.0
72-73	22.015527890112686	29.0	2.0	34.5	2.0	37.0
74-75	21.078510183575585	28.5	2.0	34.0	2.0	36.0
76-77	20.37773180201786	27.5	2.0	33.0	2.0	35.0
78-79	20.355265559702122	28.0	2.0	34.0	2.0	35.0
80-81	19.721223259360805	26.5	2.0	33.5	2.0	35.0
82-83	18.964317382994274	25.5	2.0	33.0	2.0	35.0
84-85	18.558563794742565	25.0	2.0	33.0	2.0	35.0
86-87	17.886021895455332	23.5	2.0	32.0	2.0	34.5
88-89	17.06500255159911	19.5	2.0	32.0	2.0	34.0
90-91	16.260966823723933	13.0	2.0	31.5	2.0	34.0
92-93	15.352496181452345	4.5	2.0	31.0	2.0	34.0
94-95	14.522609864729695	2.0	2.0	30.5	2.0	34.0
96-97	13.668835080901639	2.0	2.0	30.0	2.0	34.0
98-99	12.76184912205784	2.0	2.0	29.0	2.0	34.0
100	12.202008908189077	2.0	2.0	29.0	2.0	33.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.04415743034704178
1101	2	-0.030959088152112457
1101	3	-0.026771657202839805
1101	4	-0.04053737806385982
1101	5	4.3305216865263674E-4
1101	6	0.030901733442796342
1101	7	-0.0038968873047409147
1101	8	-0.037795105409720975
1101	9	-0.07848317636605628
1101	10-11	-0.057374563331130446
1101	12-13	-0.08804758310342464
1101	14-15	-0.1665154867147578
1101	16-17	-0.11444984138164216
1101	18-19	-0.15487571018633162
1101	20-21	-0.16055085903032307
1101	22-23	-0.14451817225569386
1101	24-25	-0.2635941036125331
1101	26-27	-0.26197372888763937
1101	28-29	-0.26022620209113256
1101	30-31	-0.3090235863357407
1101	32-33	-0.3419158906158941
1101	34-35	-0.38691558575727214
1101	36-37	-0.42802996730484466
1101	38-39	-0.4216236064376062
1101	40-41	-0.5354848072334448
1101	42-43	-0.5917054596930384
1101	44-45	-0.7072330834637626
1101	46-47	-0.8331920161384758
1101	48-49	-0.7641222438382052
1101	50-51	-0.8408181822408878
1101	52-53	-0.8833319334716734
1101	54-55	-0.8466230971601441
1101	56-57	-0.9772308770601832
1101	58-59	-1.1704410169099013
1101	60-61	-1.1602198399326866
1101	62-63	-1.2421168770735385
1101	64-65	-1.2669682175247203
1101	66-67	-1.3313866595607635
1101	68-69	-1.4060819373243874
1101	70-71	-1.4431572341909558
1101	72-73	-1.4796773506462735
1101	74-75	-1.5910696645769988
1101	76-77	-1.4949095538867923
1101	78-79	-1.3876370088783894
1101	80-81	-1.3293154504003795
1101	82-83	-1.4449902086160407
1101	84-85	-1.8279842537708646
1101	86-87	-1.839962126274182
1101	88-89	-1.7121976150049036
1101	90-91	-1.5077418227923918
1101	92-93	-1.377595218735582
1101	94-95	-1.3446304709512553
1101	96-97	-1.3822804051039679
1101	98-99	-1.20146844792845
1101	100	-1.0302992821264727
1102	1	0.00899067111800278
1102	2	0.01142510589525969
1102	3	0.02552419673786588
1102	4	0.035064665600202716
1102	5	0.08798769128397765
1102	6	0.0767547847683403
1102	7	0.03854448539248523
1102	8	0.013198253215307432
1102	9	0.05188092595292204
1102	10-11	0.0303623378770439
1102	12-13	0.009391117891148326
1102	14-15	0.007902771107751505
1102	16-17	0.007333291958332211
1102	18-19	-0.04375953885971029
1102	20-21	-0.06589544191905361
1102	22-23	-0.08917295940490533
1102	24-25	-0.11429190730498107
1102	26-27	-0.14321164413886578
1102	28-29	-0.15975920594311788
1102	30-31	-0.12477526269634609
1102	32-33	-0.15945249970555153
1102	34-35	-0.21918504748563805
1102	36-37	-0.2911630552871429
1102	38-39	-0.3587075028095228
1102	40-41	-0.4977780270853458
1102	42-43	-0.4646619125506639
1102	44-45	-0.42868542316881886
1102	46-47	-0.5028824626975492
1102	48-49	-0.4508518768911465
1102	50-51	-0.543247352701016
1102	52-53	-0.5513628389304444
1102	54-55	-0.525590648350299
1102	56-57	-0.6580044647082381
1102	58-59	-0.7488420691256081
1102	60-61	-0.8316514732326716
1102	62-63	-0.8647007261623223
1102	64-65	-0.8304417832096433
1102	66-67	-0.976297236886694
1102	68-69	-1.0890827470254898
1102	70-71	-1.0576165845662473
1102	72-73	-1.0601418068000719
1102	74-75	-1.0508109561090535
1102	76-77	-0.9425496976399756
1102	78-79	-0.8404421503664672
1102	80-81	-0.8375713025979792
1102	82-83	-0.8312210744079884
1102	84-85	-1.1263796980451701
1102	86-87	-1.226639655948972
1102	88-89	-1.1208429640274247
1102	90-91	-1.006112905602592
1102	92-93	-0.9194979011260038
1102	94-95	-0.8527372943524814
1102	96-97	-0.8612505013248359
1102	98-99	-0.7151476950253031
1102	100	-0.5910159811630358
1103	1	0.009476952613425738
1103	2	0.0024682532112549893
1103	3	0.04211861300765918
1103	4	0.05858086562748355
1103	5	0.10301308527473196
1103	6	0.030593733393779132
1103	7	0.003610741670414086
1103	8	0.007018961942748092
1103	9	0.04161156209080019
1103	10-11	0.019160979843199755
1103	12-13	-0.008456665149317644
1103	14-15	-0.0148734457286821
1103	16-17	0.017918690949699112
1103	18-19	-0.03822891940532713
1103	20-21	-0.07069411159332617
1103	22-23	-0.09898869053900938
1103	24-25	-0.08231445356751976
1103	26-27	-0.07402814527701196
1103	28-29	-0.05988772596280256
1103	30-31	-0.14299049081422055
1103	32-33	-0.13242299881572706
1103	34-35	-0.1164273646057552
1103	36-37	-0.21567395452662907
1103	38-39	-0.246688341305898
1103	40-41	-0.16958188255180318
1103	42-43	-0.19746635647800304
1103	44-45	-0.23004745083045464
1103	46-47	-0.2726369067384624
1103	48-49	-0.2934250962228937
1103	50-51	-0.3009805115757729
1103	52-53	-0.5040915890041759
1103	54-55	-0.40064493334672235
1103	56-57	-0.41121329147939534
1103	58-59	-0.4690142803673325
1103	60-61	-0.534218990405563
1103	62-63	-0.5138769784620933
1103	64-65	-0.49335734573576673
1103	66-67	-0.6431065629748929
1103	68-69	-0.7576788834756378
1103	70-71	-0.7845389511683472
1103	72-73	-0.7856000683194146
1103	74-75	-0.7697386593499189
1103	76-77	-0.7798499678328241
1103	78-79	-0.6678521962070185
1103	80-81	-0.6068946979794383
1103	82-83	-0.647957789143117
1103	84-85	-0.8582082643640447
1103	86-87	-1.0003100412152683
1103	88-89	-1.0146594921798133
1103	90-91	-0.9634617348118013
1103	92-93	-0.8832903295945211
1103	94-95	-0.8105540262389663
1103	96-97	-0.8062253756627804
1103	98-99	-0.6787568108929509
1103	100	-0.5536976125442461
1104	1	0.09123973159178789
1104	2	0.08425400594192567
1104	3	0.06740168583768025
1104	4	0.056724799680260674
1104	5	0.1088043225370896
1104	6	0.10940247864515129
1104	7	0.11761220425023566
1104	8	0.10717573609004205
1104	9	0.10159962912351972
1104	10-11	0.08180398361631092
1104	12-13	0.07025944576780319
1104	14-15	0.08691421413168143
1104	16-17	0.12775841346763173
1104	18-19	0.08704380287444735
1104	20-21	0.06703753382216604
1104	22-23	0.10150215738393342
1104	24-25	0.027252573612805975
1104	26-27	0.03176070661221786
1104	28-29	0.06627492086973064
1104	30-31	0.02688740979782267
1104	32-33	0.03509054821574864
1104	34-35	0.05766097395299141
1104	36-37	0.0702429976250798
1104	38-39	0.03636658068919019
1104	40-41	-0.027436252235446545
1104	42-43	2.0586642219200257E-4
1104	44-45	-0.027930757164362774
1104	46-47	-0.12825187114671976
1104	48-49	-0.07245875664494505
1104	50-51	-0.11600119886839622
1104	52-53	-0.1334226066614086
1104	54-55	-0.11406577112486715
1104	56-57	-0.09984635852616464
1104	58-59	-0.15599532714846376
1104	60-61	-0.1467815449389569
1104	62-63	-0.1182868301161335
1104	64-65	-0.1187287261053811
1104	66-67	-0.201879835503064
1104	68-69	-0.2728837229212857
1104	70-71	-0.2952948010904244
1104	72-73	-0.20727007662818409
1104	74-75	-0.2535499255965945
1104	76-77	-0.29239962568124866
1104	78-79	-0.191064706221038
1104	80-81	-0.18944941093295142
1104	82-83	-0.23275135188773888
1104	84-85	-0.3850727020973217
1104	86-87	-0.4076912625000837
1104	88-89	-0.4157297145695331
1104	90-91	-0.365633014614982
1104	92-93	-0.23841516916029803
1104	94-95	-0.22822925029951513
1104	96-97	-0.3365936439336714
1104	98-99	-0.23146370997883814
1104	100	-0.12773335214952297
1105	1	-0.041931700933471205
1105	2	-0.06701267701214064
1105	3	-0.054223812324309506
1105	4	-0.06645376164930639
1105	5	-0.03923876957235706
1105	6	-0.05752713657570041
1105	7	-0.06912245059125866
1105	8	-0.06679730465071998
1105	9	-0.10696927524782751
1105	10-11	-0.06578026011712268
1105	12-13	-0.047218002420798655
1105	14-15	-0.06268735710720108
1105	16-17	-0.020553961087451
1105	18-19	-0.012153338328431573
1105	20-21	0.021378734967569812
1105	22-23	0.019732971838344326
1105	24-25	-4.839971001615595E-4
1105	26-27	0.027424364645888488
1105	28-29	0.06732238770406695
1105	30-31	0.11419042857341566
1105	32-33	0.10295940406724924
1105	34-35	0.17437497743405572
1105	36-37	0.1846057839736588
1105	38-39	0.22956244275895443
1105	40-41	0.2236380557110209
1105	42-43	0.3668365888795009
1105	44-45	0.3373967221597738
1105	46-47	0.37940805201924377
1105	48-49	0.4550637968322029
1105	50-51	0.49577774838004984
1105	52-53	0.5402824450638519
1105	54-55	0.6346758815369924
1105	56-57	0.7170464960950476
1105	58-59	0.7012607874446459
1105	60-61	0.8027732228770574
1105	62-63	0.9091745679847811
1105	64-65	1.0155207240069402
1105	66-67	0.9861283123377866
1105	68-69	0.9928558984651481
1105	70-71	1.0180998326803703
1105	72-73	1.0812038272747415
1105	74-75	1.1017286700422844
1105	76-77	1.066984539105981
1105	78-79	1.1547249424349921
1105	80-81	1.1155623622407305
1105	82-83	1.1483961684934378
1105	84-85	1.0965989527192974
1105	86-87	1.0245167297222828
1105	88-89	1.1455110794382968
1105	90-91	1.2679367467269351
1105	92-93	1.373819493453471
1105	94-95	1.4877045045144843
1105	96-97	1.4157141413065553
1105	98-99	1.4832753619398975
1105	100	1.5273626159017653
1106	1	-0.06607114934180203
1106	2	-0.06806273063914503
1106	3	-0.03282312566492962
1106	4	-0.03547160011682848
1106	5	0.006971241362798253
1106	6	-0.0011014346113640272
1106	7	-0.032750852299720634
1106	8	-0.02485196221014263
1106	9	-0.1461793902152806
1106	10-11	-0.0870057604998209
1106	12-13	-0.08781801840292758
1106	14-15	-0.07815378506325743
1106	16-17	-0.025233276404584615
1106	18-19	-0.09332076428326985
1106	20-21	-0.10494696877594833
1106	22-23	-0.09257298785596646
1106	24-25	-0.08855138188919653
1106	26-27	-0.10768188264852796
1106	28-29	-0.06801089059691634
1106	30-31	-0.05508978279605259
1106	32-33	-0.019055058991284568
1106	34-35	0.01314374057220391
1106	36-37	-0.00558917220365629
1106	38-39	-0.02473811693890582
1106	40-41	0.013187372709843714
1106	42-43	0.06255087815162597
1106	44-45	0.06756716580933997
1106	46-47	0.0029389494066123234
1106	48-49	0.10538824504588362
1106	50-51	0.15214556482055386
1106	52-53	0.12462133579352752
1106	54-55	0.053818188775867526
1106	56-57	-0.004216244416721082
1106	58-59	0.09893709494536296
1106	60-61	0.1449206141483188
1106	62-63	0.16794599025644175
1106	64-65	0.2099266915083149
1106	66-67	0.136759139684564
1106	68-69	0.11760591988918634
1106	70-71	0.09091296238981528
1106	72-73	0.022169663804966433
1106	74-75	0.08322981583532396
1106	76-77	0.07570153240146738
1106	78-79	0.1856718952919607
1106	80-81	0.309989451761286
1106	82-83	0.27106181598235324
1106	84-85	0.02937884935666446
1106	86-87	-0.07318902301885188
1106	88-89	-0.05210943771465182
1106	90-91	-0.0356560725270505
1106	92-93	-0.10768117375700292
1106	94-95	-0.061606369148782036
1106	96-97	-0.13175764625782094
1106	98-99	-0.11126637326231581
1106	100	-0.029659219060738096
1107	1	-0.09733438665024963
1107	2	-0.12795022193142458
1107	3	-0.07984945203135396
1107	4	-0.0830524771488399
1107	5	-0.05440492319196011
1107	6	-0.048576712046411785
1107	7	-0.11789716675205142
1107	8	-0.11578183373109141
1107	9	-0.15750568695476375
1107	10-11	-0.07644824360270519
1107	12-13	-0.11703688593244976
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2207	68-69	0.12935264676367453
2207	70-71	0.019838182582425645
2207	72-73	0.09514471978300776
2207	74-75	0.20419360489982807
2207	76-77	0.12853627504244258
2207	78-79	0.04861270082079017
2207	80-81	0.08535241893069667
2207	82-83	-0.012629142924740933
2207	84-85	0.23676699511185362
2207	86-87	0.15287653330664241
2207	88-89	0.16942873659932545
2207	90-91	0.32687505480834034
2207	92-93	0.26953240017802216
2207	94-95	0.28550318944133757
2207	96-97	0.4257826379930272
2207	98-99	0.38800278395266297
2207	100	0.302017417507626
2208	1	-0.32128087459885535
2208	2	-0.32679890922575794
2208	3	-0.33210172163771645
2208	4	-0.3161630543534031
2208	5	-0.4512744085788185
2208	6	-0.3928840354412415
2208	7	-0.3996465118216719
2208	8	-0.36913907434919935
2208	9	-0.3620731423422754
2208	10-11	-0.362568346666869
2208	12-13	-0.3430073331189476
2208	14-15	-0.38098368506528857
2208	16-17	-0.4160157638830384
2208	18-19	-0.41655019008796046
2208	20-21	-0.41132185709155067
2208	22-23	-0.47757025278257714
2208	24-25	-0.4080921430959137
2208	26-27	-0.35503024885534273
2208	28-29	-0.3533724398797631
2208	30-31	-0.302447422239311
2208	32-33	-0.27104878615664774
2208	34-35	-0.2747554074881009
2208	36-37	-0.26501168057998115
2208	38-39	-0.17186121588005676
2208	40-41	-0.17739224107931761
2208	42-43	-0.1136658839793796
2208	44-45	-0.016498911653393122
2208	46-47	0.030394053029223045
2208	48-49	0.05019368009142866
2208	50-51	0.06276170790032864
2208	52-53	0.12125677434197968
2208	54-55	0.1682696137722779
2208	56-57	0.3691731805905505
2208	58-59	0.4273017726067678
2208	60-61	0.48485703228279675
2208	62-63	0.29010218999295034
2208	64-65	0.4732928055304555
2208	66-67	0.5166432518712583
2208	68-69	0.7610217485918227
2208	70-71	0.8284412590018775
2208	72-73	0.9245184681510921
2208	74-75	1.0007281080272037
2208	76-77	0.9036443131330714
2208	78-79	0.9944032475217668
2208	80-81	1.1455580859190135
2208	82-83	1.170182105981315
2208	84-85	1.1898523894991406
2208	86-87	1.0595533294970672
2208	88-89	1.028281176716309
2208	90-91	0.4964140439330791
2208	92-93	0.4835097610979133
2208	94-95	0.8433633727569241
2208	96-97	0.9248810197652375
2208	98-99	0.7125470610747051
2208	100	0.3669109745746866
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	585496.0
3	324396.0
4	212310.0
5	168667.0
6	170412.0
7	189556.0
8	205152.0
9	225166.0
10	250027.0
11	278659.0
12	320722.0
13	395547.0
14	539201.0
15	668065.0
16	737671.0
17	717277.0
18	692000.0
19	653470.0
20	650325.0
21	676153.0
22	704717.0
23	741268.0
24	778087.0
25	813485.0
26	860570.0
27	919448.0
28	1001784.0
29	1092029.0
30	1211515.0
31	1360224.0
32	1563828.0
33	1861919.0
34	2339715.0
35	3117756.0
36	3751188.0
37	2606582.0
38	502752.0
39	7291.0
40	1.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.575886288334274	28.333714819556032	27.136292835948506	21.954106056161187
2	23.539572031759437	27.305689244348137	28.19616591291944	20.95857281097299
3	23.572133229348147	26.790612522736918	26.888582745267975	22.748671502646957
4	24.504587480248556	25.272705181464833	27.30311831580721	22.919589022479396
5	25.45699024055292	25.5864964510161	25.568947358931105	23.387565949499876
6	25.590993141668157	25.359091819465046	25.51135898004911	23.538556058817687
7	24.800110897991694	26.187342020599168	25.278169079839362	23.734378001569773
8	24.74046879357422	25.467559638946717	25.997359967587624	23.794611599891432
9	24.529379206804297	25.762902968309476	25.631253211723283	24.076464613162944
10-11	24.698584446233042	25.52970964942795	25.890019484602394	23.88168641973661
12-13	23.97849756895822	25.546681964366346	26.23387240351714	24.240948063158296
14-15	23.323051560187526	25.730679634941854	27.40601290026473	23.540255904605885
16-17	23.011946524267106	25.72980973045303	27.886897115744073	23.37134662953579
18-19	22.402841610566824	25.674481589317676	29.21843196848907	22.70424483162643
20-21	21.667947188168466	25.550614737011134	30.762830964004255	22.018607110816145
22-23	21.600726349712996	25.441395121927084	31.296146008535025	21.661732519824895
24-25	21.19696362118378	24.972917410977377	32.279068383826164	21.55105058401268
26-27	21.213330873827015	25.030723461472814	32.89243823715847	20.8635074275417
28-29	21.281998080294535	25.080012432016858	32.86633524503459	20.77165424265402
30-31	21.260913748331927	25.0268972576687	32.82796056626526	20.884228427734115
32-33	21.283036558864232	25.107350077648494	32.690643473431166	20.91896989005611
34-35	21.386108572013264	25.149482920045998	32.65937611814149	20.80503238979926
36-37	21.42175381496143	25.17587285507257	32.41219920553374	20.990174124432265
38-39	21.501591860241707	25.37482199880669	32.03065189027637	21.092934250675235
40-41	21.655203400858415	25.612520852044806	31.530321685427232	21.201954061669543
42-43	21.871542201298276	25.81301525543856	30.832276173047862	21.483166370215304
44-45	21.989209566425696	26.125301915582288	30.18138226456867	21.70410625342335
46-47	22.269162981110654	26.385790520408207	29.559582878533202	21.785463619947937
48-49	22.38471116545423	26.589988148124853	28.95964065553992	22.065660030881
50-51	22.48191794373039	26.891537659765145	28.409067313637888	22.21747708286657
52-53	22.61983901788475	27.06814948078855	28.07791539713361	22.234096104193092
54-55	22.784793766089564	27.08253519918871	27.846745971612215	22.285925063109506
56-57	22.764007912879432	27.23667449761732	27.683184354699815	22.316133234803434
58-59	22.889228710326677	27.375966533267544	27.551974205413554	22.182830550992225
60-61	22.86883394877841	27.445111550021693	27.38578233367855	22.300272167521342
62-63	22.9130717511628	27.6566684622626	27.197000448274057	22.233259338300545
64-65	22.98077902595862	27.850403616228338	26.97526130388079	22.19355605393226
66-67	22.98897661408785	27.969445138363515	26.754879831491756	22.286698416056883
68-69	22.938753813833	28.198241339673867	26.541483812235082	22.321521034258048
70-71	22.98814830079574	28.36624461155404	26.365783448563327	22.279823639086885
72-73	22.987436169539187	28.46662143565239	26.080294234980222	22.4656481598282
74-75	23.096224954185153	28.52280134181931	25.942693816661983	22.438279887333554
76-77	22.383317648736174	29.251735680770675	25.570040935998605	22.794905734494545
78-79	22.6671709089564	29.213859664158214	25.435833348288796	22.68313607859659
80-81	22.645396722267098	29.310538922534718	25.197842699617624	22.846221655580564
82-83	22.57753312379725	29.478265886425692	25.003840973673512	22.940360016103543
84-85	22.63443355805125	29.48304543105122	24.80898131323188	23.07353969766565
86-87	22.631571672420172	29.572687606980708	24.643300533428167	23.152440187170956
88-89	22.71351791175126	29.602664132482847	24.316668673143024	23.36714928262287
90-91	22.634945555505205	29.637835359866614	24.16623532616187	23.56098375846631
92-93	22.559253886465296	29.70058380511046	23.964114500586955	23.776047807837287
94-95	22.66447100221345	29.746087783335945	23.78459798258835	23.804843231862257
96-97	22.419909291413052	29.851741877274556	23.61837397428195	24.10997485703044
98-99	22.238418705295075	29.88005507135344	23.477906232920482	24.403619990431004
100	23.258282356666932	29.707616552560896	23.017284049836036	24.016817040936136
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2933.0
1	2309.5
2	1687.5
3	1833.0
4	2258.5
5	2932.0
6	3930.5
7	5368.0
8	7438.5
9	10035.5
10	13075.5
11	16414.5
12	19736.0
13	24750.0
14	33786.0
15	43944.0
16	77238.0
17	97520.0
18	86371.5
19	83824.5
20	93291.5
21	102463.0
22	108794.5
23	122518.0
24	137453.5
25	153410.5
26	172083.0
27	196277.0
28	227081.5
29	261590.5
30	298341.0
31	346238.0
32	411979.0
33	482339.5
34	550904.5
35	636336.5
36	737832.0
37	849738.0
38	962567.5
39	1048653.0
40	1123479.5
41	1225949.5
42	1375593.0
43	1514233.0
44	1600624.0
45	1636242.0
46	1593912.5
47	1499792.0
48	1423531.5
49	1382315.5
50	1314703.0
51	1212535.0
52	1093539.5
53	960423.5
54	847654.5
55	752301.0
56	654470.0
57	568840.5
58	505293.0
59	450949.5
60	399432.0
61	349786.5
62	302394.0
63	261570.0
64	227604.5
65	197407.5
66	169210.5
67	143892.0
68	120986.0
69	101048.0
70	83628.5
71	68593.0
72	56637.0
73	47129.5
74	38957.0
75	31993.0
76	26238.0
77	21241.5
78	17377.0
79	14171.5
80	11234.5
81	8693.0
82	6675.5
83	5037.5
84	3608.0
85	2462.5
86	1718.0
87	1190.5
88	803.0
89	542.5
90	358.5
91	236.0
92	158.5
93	105.5
94	56.5
95	30.5
96	16.5
97	7.0
98	5.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.053557470842333955
2	0.0
3	0.05254845552651407
4	0.005809213908916187
5	0.1877034017771238
6	0.1687415847163801
7	0.30925434328724977
8	0.3312343552839108
9	0.3360139015167418
10-11	0.31563444744064295
12-13	0.3137521323193182
14-15	0.3112959766163356
16-17	0.33133466674805667
18-19	0.3787303583883736
20-21	0.45323227287692186
22-23	0.4241006435540989
24-25	0.42474529222809493
26-27	0.41213112561175613
28-29	0.4784650316153707
30-31	0.43379250119289514
32-33	0.3904638493562556
34-35	0.4214040353708844
36-37	0.3742650230652935
38-39	0.39467398051319996
40-41	0.44119047167365044
42-43	0.44005459185905793
44-45	0.4373668346873857
46-47	0.44047501490731616
48-49	0.36532697657618146
50-51	0.4666091016544871
52-53	0.4645409152907744
54-55	0.47443044552068153
56-57	0.45359221401297456
58-59	0.4287843038285552
60-61	0.41423766635881865
62-63	0.4639596988661647
64-65	0.4438295482818402
66-67	0.4393081565523257
68-69	0.47155976744380224
70-71	0.440520745133618
72-73	0.2595750906690247
74-75	0.6547948835606652
76-77	0.4239546018636513
78-79	0.4583629092342633
80-81	0.25337495708365776
82-83	8.99852840131761E-5
84-85	6.9037890029781E-4
86-87	5.207345124041173E-4
88-89	6.0481912205577363E-5
90-91	2.8028203217218784E-4
92-93	2.1242427701471074E-4
94-95	1.7554506225521237E-4
96-97	1.9324708533977161E-4
98-99	0.001724472082154145
100	5.900674361519743E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.3894431E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.42548662532326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.22891517098128	31.212605934920585
2	10.15912977098956	7.807390102801652
3	3.3275472218673205	3.835878638069828
4	1.522299857965968	2.3398045132801117
5	0.8261407292574838	1.5872429771359127
6	0.5269663083523316	1.2149362100153094
7	0.3357024675682519	0.9029671474342313
8	0.2542937690622522	0.781708945760369
9	0.19356451095784905	0.6694029474253259
>10	1.2791302843721077	9.641956566083698
>50	0.13067070642359688	3.4614472705283554
>100	0.14665076545547684	13.474101281769055
>500	0.057921060976628164	15.309944139221068
>1k	0.010829910001874575	5.298979855548589
>5k	6.894167459852357E-5	0.19023900707387723
>10k+	1.6852409344699296E-4	2.2713944629318803
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	126388	0.37288721560187865	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	123783	0.3652015872459992	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59755	0.17629739823630614	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53365	0.15744474365125055	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40670	0.11999021314150397	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39640	0.11695136584532133	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34153	0.10076286573449189	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.9503371807598717E-6	2.9503371807598717E-6	0.0
2	0.0	0.0	2.9503371807598717E-6	5.900674361519743E-6	0.0
3	0.0	0.0	2.9503371807598717E-6	5.900674361519743E-6	0.0
4	0.0	2.9503371807598717E-6	2.9503371807598717E-6	5.900674361519743E-6	0.0
5	2.9503371807598717E-6	2.9503371807598717E-6	2.9503371807598717E-6	8.851011542279616E-6	0.0
6	2.9503371807598717E-6	2.9503371807598717E-6	2.9503371807598717E-6	8.851011542279616E-6	2.9503371807598717E-6
7	2.9503371807598717E-6	2.9503371807598717E-6	2.9503371807598717E-6	1.475168590379936E-5	2.9503371807598717E-6
8	2.9503371807598717E-6	8.851011542279616E-6	2.9503371807598717E-6	1.7702023084559232E-5	2.9503371807598717E-6
9	1.1801348723039487E-5	1.7702023084559232E-5	2.9503371807598717E-6	1.7702023084559232E-5	5.900674361519743E-6
10-11	3.8354383349878336E-5	2.2127528855699037E-5	2.9503371807598717E-6	2.2127528855699037E-5	5.900674361519743E-6
12-13	1.3866584749571396E-4	2.6553034626838846E-5	2.9503371807598717E-6	2.8028203217218782E-5	8.851011542279616E-6
14-15	4.36649902752461E-4	2.950337180759872E-5	2.9503371807598717E-6	3.540404616911846E-5	8.851011542279616E-6
16-17	9.278810433489795E-4	3.245370898835859E-5	2.9503371807598717E-6	4.13047205306382E-5	8.851011542279616E-6
18-19	0.0018513365809268195	3.5404046169118464E-5	2.9503371807598717E-6	4.4255057711398075E-5	8.851011542279616E-6
20-21	0.0030772016795325464	3.982955194025827E-5	2.9503371807598717E-6	4.720539489215795E-5	8.851011542279616E-6
22-23	0.004847403987988469	4.13047205306382E-5	2.9503371807598717E-6	4.720539489215795E-5	1.032618013265955E-5
24-25	0.007048355524835334	4.13047205306382E-5	2.9503371807598717E-6	5.015573207291782E-5	1.1801348723039487E-5
26-27	0.009548766285529326	4.2779889121018135E-5	2.9503371807598717E-6	5.015573207291782E-5	1.1801348723039487E-5
28-29	0.012915101008776339	4.4255057711398075E-5	2.9503371807598717E-6	5.163090066329776E-5	1.1801348723039487E-5
30-31	0.015568929302869843	4.868056348253788E-5	2.9503371807598717E-6	5.6056406434437565E-5	1.475168590379936E-5
32-33	0.017182763740745493	5.900674361519744E-5	2.9503371807598717E-6	5.6056406434437565E-5	1.475168590379936E-5
34-35	0.018924937845984195	6.195708079595732E-5	2.9503371807598717E-6	5.6056406434437565E-5	1.475168590379936E-5
36-37	0.020822004653212794	6.785775515747705E-5	2.9503371807598717E-6	5.7531575024817504E-5	1.475168590379936E-5
38-39	0.02247861898020946	7.080809233823693E-5	2.9503371807598717E-6	6.19570807959573E-5	1.475168590379936E-5
40-41	0.024149984993109927	7.37584295189968E-5	2.9503371807598717E-6	6.19570807959573E-5	1.475168590379936E-5
42-43	0.02606475382342309	7.965910388051654E-5	2.9503371807598717E-6	6.490741797671718E-5	1.6226854494179296E-5
44-45	0.028196372436522094	8.26094410612764E-5	2.9503371807598717E-6	6.638258656709712E-5	1.7702023084559232E-5
46-47	0.030658428813866205	8.555977824203628E-5	2.9503371807598717E-6	6.785775515747705E-5	1.7702023084559232E-5
48-49	0.03343764643814201	8.851011542279615E-5	2.9503371807598717E-6	7.523359810937673E-5	1.7702023084559232E-5
50-51	0.03680398116138902	8.851011542279615E-5	2.9503371807598717E-6	7.670876669975666E-5	1.7702023084559232E-5
52-53	0.04068367455408825	8.851011542279615E-5	4.425505771139807E-6	7.965910388051654E-5	1.7702023084559232E-5
54-55	0.04545289460678659	8.851011542279615E-5	5.900674361519743E-6	7.965910388051654E-5	1.7702023084559232E-5
56-57	0.051313739416366064	9.588595837469583E-5	5.900674361519743E-6	8.113427247089647E-5	1.9177191674939168E-5
58-59	0.05850813663164901	1.0178663273621557E-4	5.900674361519743E-6	9.146045260355603E-5	2.6553034626838846E-5
60-61	0.06688709422500705	1.032618013265955E-4	7.37584295189968E-6	9.44107897843159E-5	2.6553034626838846E-5
62-63	0.07673531973438351	1.0916247568811525E-4	8.851011542279616E-6	9.588595837469583E-5	2.6553034626838846E-5
64-65	0.08846733553367514	1.1211281286887513E-4	1.1801348723039487E-5	9.736112696507577E-5	2.6553034626838846E-5
66-67	0.10312461064769018	1.1801348723039487E-4	1.3276517313419423E-5	1.0031146414583564E-4	2.6553034626838846E-5
68-69	0.12019526157556679	1.239141615919146E-4	1.475168590379936E-5	1.1358798145925506E-4	2.8028203217218782E-5
70-71	0.13965126011408777	1.3276517313419423E-4	1.475168590379936E-5	1.1948865582077481E-4	3.245370898835859E-5
72-73	0.1630592353062366	1.357155103149541E-4	1.475168590379936E-5	1.2096382441115474E-4	3.245370898835859E-5
74-75	0.18998253724926079	1.3866584749571396E-4	1.475168590379936E-5	1.268644987726745E-4	3.245370898835859E-5
76-77	0.22183880295851552	1.4161618467647386E-4	1.475168590379936E-5	1.4161618467647383E-4	3.5404046169118464E-5
78-79	0.2583875799537688	1.430913532668538E-4	1.6226854494179296E-5	1.475168590379936E-4	3.5404046169118464E-5
80-81	0.2994665996900789	1.4899202762837352E-4	1.7702023084559232E-5	1.475168590379936E-4	3.68792147594984E-5
82-83	0.3452927119502316	1.5046719621875345E-4	1.7702023084559232E-5	1.5046719621875345E-4	3.835438334987833E-5
84-85	0.39682182598079313	1.5489270198989325E-4	1.7702023084559232E-5	1.5194236480913339E-4	3.982955194025827E-5
86-87	0.4530095224197745	1.6226854494179294E-4	1.9177191674939168E-5	1.637437135321729E-4	4.13047205306382E-5
88	0.4991616469384012	1.6226854494179294E-4	2.06523602653191E-5	1.681692193033127E-4	4.13047205306382E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	36295	0.0	57.3707	1
TATCTCG	38995	0.0	52.129055	2
ATGGATC	46355	0.0	45.686615	3
ATCTCGA	46030	0.0	43.427616	3
ATATGGA	50570	0.0	42.309437	1
GGATCCG	48795	0.0	42.1751	5
TATGGAT	51880	0.0	41.137688	2
TGGATCC	51645	0.0	40.820694	4
TCTCGAG	51330	0.0	38.289814	4
CTCGAGG	59935	0.0	32.635746	5
GATCCGG	69010	0.0	29.769117	6
TCGAGGG	67800	0.0	28.665905	6
ATCCGGC	81410	0.0	24.651451	7
CGAGGGC	88045	0.0	21.775116	7
TCCGGCG	101445	0.0	19.671797	8
AGGGCGC	104145	0.0	18.183554	9
CCGGCGC	116505	0.0	17.048445	9
GAGGGCG	113000	0.0	16.833311	8
GCGCCGT	120415	0.0	14.623107	9
CGGCGCG	120505	0.0	14.567777	5
>>END_MODULE