FastQCFastQC Report
Wed 25 May 2016
SRR490996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39065873
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT2152320.5509463464441202No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT2022800.5177920892744416No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT911520.23332897232323466No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT836820.21420742344603433No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA776790.19884107031218784No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT571460.14628112880006547No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT513700.1314958454915368No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453800.11616276948425036No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT392060.10035869414719081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC528050.065.040831
TATCTCG548500.061.0060272
TATGGAT630100.050.8766672
ATATGGA637750.050.6484381
ATGGATC640800.049.8810463
ATCTCGA700350.047.0213173
GGATCCG667950.046.0339365
TGGATCC720050.044.131544
TCTCGAG785600.041.2264144
CTCGAGG892750.036.1047135
GATCCGG915300.033.4661876
TCGAGGG989200.032.4661526
ATCCGGC1067800.028.0787777
CGAGGGC1211100.026.0514497
TCCGGCG1318000.022.6308968
AGGGCGC1422750.021.941679
GAGGGCG1485450.021.1166558
CCGGCGC1513200.019.5098699
GCGCCGT1558350.014.0359479
CGGCGCG1588400.013.8554985