##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490996_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39065873
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16298570877963	31.0	31.0	34.0	28.0	34.0
2	31.436011400538778	34.0	31.0	34.0	28.0	34.0
3	31.534674266718678	34.0	31.0	34.0	28.0	34.0
4	34.93826941996151	37.0	35.0	37.0	32.0	37.0
5	34.63616707093683	37.0	35.0	37.0	32.0	37.0
6	34.52097130915262	37.0	35.0	37.0	31.0	37.0
7	34.425866817311366	37.0	35.0	37.0	31.0	37.0
8	34.29710496934242	37.0	35.0	37.0	30.0	37.0
9	35.75729824340544	39.0	37.0	39.0	30.0	39.0
10-11	35.58680466452138	39.0	37.0	39.0	30.0	39.0
12-13	35.362137344274885	39.0	35.0	39.0	30.0	39.0
14-15	36.42172401983696	40.0	36.5	41.0	29.5	41.0
16-17	36.436154709764196	40.0	37.0	41.0	29.5	41.0
18-19	36.32390108113032	40.0	37.0	41.0	28.5	41.0
20-21	36.13715423689623	40.0	36.5	41.0	27.5	41.0
22-23	35.93861093287228	40.0	36.0	41.0	27.0	41.0
24-25	35.701100510412246	40.0	36.0	41.0	26.5	41.0
26-27	35.35868216742526	39.0	35.0	41.0	25.5	41.0
28-29	34.981408005396425	39.0	35.0	41.0	24.5	41.0
30-31	34.573305874925666	39.0	35.0	40.5	22.0	41.0
32-33	34.14858999823196	38.0	34.0	40.0	18.0	41.0
34-35	33.763145418508884	38.0	33.5	40.0	15.5	41.0
36-37	33.26175796711365	38.0	33.0	40.0	8.5	41.0
38-39	32.748128871969655	38.0	33.0	40.0	2.0	41.0
40-41	32.2300617344453	38.0	32.0	40.0	2.0	41.0
42-43	31.642585153543095	38.0	31.0	40.0	2.0	41.0
44-45	31.06738637326753	37.0	30.0	40.0	2.0	41.0
46-47	30.467658575043234	37.0	29.0	40.0	2.0	41.0
48-49	29.83127549715835	36.0	27.5	39.5	2.0	40.5
50-51	29.215242137299736	36.0	26.0	39.0	2.0	40.0
52-53	28.61315020401566	35.0	23.5	39.0	2.0	40.0
54-55	27.958535535606742	35.0	22.0	39.0	2.0	40.0
56-57	27.271552654154178	34.0	18.5	38.0	2.0	40.0
58-59	26.69087706039489	34.0	12.5	38.0	2.0	40.0
60-61	25.981091834297416	33.0	7.5	38.0	2.0	40.0
62-63	25.255973608986032	32.5	2.0	37.0	2.0	39.5
64-65	24.577510196687527	32.0	2.0	36.5	2.0	39.0
66-67	23.811991991065963	31.0	2.0	36.0	2.0	39.0
68-69	23.027076663562593	30.5	2.0	35.0	2.0	38.0
70-71	22.241197809658573	29.5	2.0	35.0	2.0	38.0
72-73	21.300150645551938	28.5	2.0	34.0	2.0	37.0
74-75	20.372111932581156	26.5	2.0	34.0	2.0	36.0
76-77	19.6803226437561	26.0	2.0	33.0	2.0	35.0
78-79	19.655113223759265	26.0	2.0	33.0	2.0	35.0
80-81	19.023006487017454	25.5	2.0	33.0	2.0	35.0
82-83	18.268327563036927	24.5	2.0	32.5	2.0	35.0
84-85	17.86584119085218	23.5	2.0	32.0	2.0	35.0
86-87	17.198525718342452	19.5	2.0	32.0	2.0	34.0
88-89	16.381263142385173	14.5	2.0	31.5	2.0	34.0
90-91	15.582094965598234	4.5	2.0	31.0	2.0	34.0
92-93	14.684834638150797	2.0	2.0	31.0	2.0	34.0
94-95	13.872429895525437	2.0	2.0	30.0	2.0	34.0
96-97	13.026667098928009	2.0	2.0	29.5	2.0	34.0
98-99	12.14586510072359	2.0	2.0	29.0	2.0	33.5
100	11.633644331972308	2.0	2.0	28.0	2.0	33.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.040397261441526666
1101	2	-0.02675317662717802
1101	3	-0.011454153408053003
1101	4	-0.04171615865102751
1101	5	0.009021184154235584
1101	6	0.039847943257043994
1101	7	0.014458041784067177
1101	8	-0.03557946986231997
1101	9	-0.07008690568066811
1101	10-11	-0.052112096232292515
1101	12-13	-0.07331859752888192
1101	14-15	-0.14796901289587794
1101	16-17	-0.08562767841733887
1101	18-19	-0.15372617758144003
1101	20-21	-0.13532699721703523
1101	22-23	-0.1409883256191975
1101	24-25	-0.2582435237358567
1101	26-27	-0.2402886904521111
1101	28-29	-0.23176390458007035
1101	30-31	-0.29591039072226977
1101	32-33	-0.3260131699410209
1101	34-35	-0.40586333670987784
1101	36-37	-0.41735779058257094
1101	38-39	-0.42739699569488465
1101	40-41	-0.5582995389639436
1101	42-43	-0.62042592357761
1101	44-45	-0.6978799693983326
1101	46-47	-0.7998001903499592
1101	48-49	-0.739424309302624
1101	50-51	-0.8144915097798204
1101	52-53	-0.8522274533872363
1101	54-55	-0.8455265513314423
1101	56-57	-1.0117757958419595
1101	58-59	-1.164509765860874
1101	60-61	-1.1712220049006525
1101	62-63	-1.2543814162004878
1101	64-65	-1.2797916305660166
1101	66-67	-1.3839340347064457
1101	68-69	-1.4532084676904695
1101	70-71	-1.488644867100966
1101	72-73	-1.5161234209791736
1101	74-75	-1.6811963378812358
1101	76-77	-1.5700738949777069
1101	78-79	-1.4574948651386457
1101	80-81	-1.4029748994480613
1101	82-83	-1.473816486567678
1101	84-85	-1.8054516101691487
1101	86-87	-1.8140074784402014
1101	88-89	-1.7041803287760544
1101	90-91	-1.521400109249198
1101	92-93	-1.3893080964062001
1101	94-95	-1.3549056208942662
1101	96-97	-1.3923764765726538
1101	98-99	-1.2117586577658184
1101	100	-1.0527914296149241
1102	1	0.01162851074067106
1102	2	0.028216937349849758
1102	3	0.02264863429279984
1102	4	0.0390320587393731
1102	5	0.09756800499443585
1102	6	0.0883572132301893
1102	7	0.04655864343676086
1102	8	0.02566099971633662
1102	9	0.05015458684016494
1102	10-11	0.05576193488855807
1102	12-13	0.03423479435193144
1102	14-15	0.015422638471022765
1102	16-17	0.05305346138786149
1102	18-19	-0.007765026096336669
1102	20-21	-0.0448533395420867
1102	22-23	-0.05157471409314951
1102	24-25	-0.06500618338053243
1102	26-27	-0.09449969971585404
1102	28-29	-0.13141377072198424
1102	30-31	-0.10512285753156192
1102	32-33	-0.1510168125059579
1102	34-35	-0.20122832285955639
1102	36-37	-0.25802902070945777
1102	38-39	-0.29622903770894027
1102	40-41	-0.46570787409400793
1102	42-43	-0.42611104439635383
1102	44-45	-0.40472419667410264
1102	46-47	-0.4879534177731628
1102	48-49	-0.47549377707132834
1102	50-51	-0.5508212935127332
1102	52-53	-0.5848982838893413
1102	54-55	-0.5552693398478361
1102	56-57	-0.6453614840268891
1102	58-59	-0.7475049644193987
1102	60-61	-0.8520339478228074
1102	62-63	-0.8783767614072602
1102	64-65	-0.8201923317258633
1102	66-67	-0.9546825553278353
1102	68-69	-1.0651037884018315
1102	70-71	-1.0480935088214665
1102	72-73	-1.0740022746471283
1102	74-75	-1.0615714089035855
1102	76-77	-0.9443826033874139
1102	78-79	-0.8767165745700289
1102	80-81	-0.8578651747198691
1102	82-83	-0.8319761359827638
1102	84-85	-1.1225887948541171
1102	86-87	-1.217985571382851
1102	88-89	-1.093807457841102
1102	90-91	-0.985810291760588
1102	92-93	-0.8949311990561899
1102	94-95	-0.8226711468616248
1102	96-97	-0.8164781061659543
1102	98-99	-0.6838079388194682
1102	100	-0.5857615669318026
1103	1	0.02812114223887363
1103	2	0.02889547244902957
1103	3	0.05797258932610205
1103	4	0.0881751818773111
1103	5	0.1520098075192564
1103	6	0.05607190319168609
1103	7	0.018768121931472592
1103	8	0.04033000787026708
1103	9	0.06037414487848736
1103	10-11	0.046052906690285056
1103	12-13	0.02870515623370551
1103	14-15	0.030853323373385422
1103	16-17	0.07426218620145875
1103	18-19	0.02261683489091837
1103	20-21	-0.01843611312649074
1103	22-23	-0.019009530942625474
1103	24-25	-0.01330729553280463
1103	26-27	6.992912192842482E-4
1103	28-29	0.012527184758013732
1103	30-31	-0.06235308920683735
1103	32-33	-0.04587161784193228
1103	34-35	-0.04973648130994235
1103	36-37	-0.16087895222648285
1103	38-39	-0.16034061951187084
1103	40-41	-0.1228403345646214
1103	42-43	-0.16101433724552905
1103	44-45	-0.1922243679898834
1103	46-47	-0.18987761770272016
1103	48-49	-0.22334283765411556
1103	50-51	-0.18580342344815648
1103	52-53	-0.4083461310579999
1103	54-55	-0.3230887071392381
1103	56-57	-0.3030169411158177
1103	58-59	-0.38062855306173304
1103	60-61	-0.4086862243698768
1103	62-63	-0.3489354220444767
1103	64-65	-0.38816323290702215
1103	66-67	-0.49948748881072547
1103	68-69	-0.6221362864508642
1103	70-71	-0.6495442806281915
1103	72-73	-0.6586194660736773
1103	74-75	-0.6156654356462958
1103	76-77	-0.6248950327335372
1103	78-79	-0.5164899715932876
1103	80-81	-0.4593178594399987
1103	82-83	-0.4706156310722207
1103	84-85	-0.6645587222844824
1103	86-87	-0.8038684099670341
1103	88-89	-0.8480488398581265
1103	90-91	-0.7992228435439994
1103	92-93	-0.7387602914232687
1103	94-95	-0.7188742981565674
1103	96-97	-0.6901511828056748
1103	98-99	-0.6059422137476904
1103	100	-0.4850201948280688
1104	1	0.07027753790944402
1104	2	0.06308054936769025
1104	3	0.04959899103346643
1104	4	0.03460922386489074
1104	5	0.08465766392975382
1104	6	0.1000272468471195
1104	7	0.09654817413672134
1104	8	0.09141434617864519
1104	9	0.08387436836769524
1104	10-11	0.06274340472821649
1104	12-13	0.060699143360089636
1104	14-15	0.043786455034535265
1104	16-17	0.09657857485604637
1104	18-19	0.05825754598995303
1104	20-21	0.01745496356839027
1104	22-23	0.07055349723633242
1104	24-25	0.0037444852943693263
1104	26-27	-0.0012449270252616884
1104	28-29	0.07921956330401514
1104	30-31	0.03525453924547861
1104	32-33	0.06012003685145828
1104	34-35	0.08385805178666317
1104	36-37	0.08335343825998365
1104	38-39	0.04378959464078491
1104	40-41	-0.02848448122127678
1104	42-43	0.01837037432774835
1104	44-45	0.0187797459833412
1104	46-47	-0.0631501354619175
1104	48-49	-0.04153669419136108
1104	50-51	-0.08828530503305387
1104	52-53	-0.1124974545321713
1104	54-55	-0.06353012456213847
1104	56-57	-0.05099413539944564
1104	58-59	-0.10048819334347314
1104	60-61	-0.11551949430524999
1104	62-63	-0.043579716445432126
1104	64-65	-0.05084514453344724
1104	66-67	-0.13489675912608945
1104	68-69	-0.21838975076041933
1104	70-71	-0.22340159478472543
1104	72-73	-0.13008552632075876
1104	74-75	-0.1932215831888513
1104	76-77	-0.23577988795392102
1104	78-79	-0.12554790817942418
1104	80-81	-0.11357041748161834
1104	82-83	-0.18701747114941725
1104	84-85	-0.375631473254078
1104	86-87	-0.4234624152439821
1104	88-89	-0.422839029227541
1104	90-91	-0.3507983267568431
1104	92-93	-0.23751708716578612
1104	94-95	-0.2528321560587532
1104	96-97	-0.35626559919546175
1104	98-99	-0.23657997809390707
1104	100	-0.14227437494578687
1105	1	-0.03508677360110113
1105	2	-0.06078139060540977
1105	3	-0.055266182273548736
1105	4	-0.07120781871777382
1105	5	-0.03793732867630695
1105	6	-0.05545286611611289
1105	7	-0.07867622340656055
1105	8	-0.06273437233571144
1105	9	-0.09281158709796955
1105	10-11	-0.04567202064482956
1105	12-13	-0.014845062652248942
1105	14-15	-0.016655665137236042
1105	16-17	-0.008313669860122275
1105	18-19	-0.004397300706912688
1105	20-21	0.02954558032600829
1105	22-23	0.05685209969692551
1105	24-25	0.044433028668642294
1105	26-27	0.06647627606518114
1105	28-29	0.08748395219642902
1105	30-31	0.12256940603163002
1105	32-33	0.158579292315423
1105	34-35	0.23029264466575228
1105	36-37	0.247195307936785
1105	38-39	0.2774990984733421
1105	40-41	0.25522502931497826
1105	42-43	0.4327395696139824
1105	44-45	0.4399065119606327
1105	46-47	0.4814909903229747
1105	48-49	0.5451704048820147
1105	50-51	0.5856635453310375
1105	52-53	0.6266049843968808
1105	54-55	0.7256737860391347
1105	56-57	0.805326232005573
1105	58-59	0.8040248255494191
1105	60-61	0.9078401420184754
1105	62-63	1.0029402366116926
1105	64-65	1.1141679036921062
1105	66-67	1.066617353649196
1105	68-69	1.057639225516457
1105	70-71	1.093122752511622
1105	72-73	1.1457084398721697
1105	74-75	1.2140991294311654
1105	76-77	1.1836692146253007
1105	78-79	1.295528367898445
1105	80-81	1.2580076392902413
1105	82-83	1.2786692303841782
1105	84-85	1.19700497964336
1105	86-87	1.1223138836152131
1105	88-89	1.1930929143973437
1105	90-91	1.327222558911556
1105	92-93	1.4119150818216113
1105	94-95	1.5226994542352887
1105	96-97	1.4351661325459482
1105	98-99	1.5036835379290086
1105	100	1.5430887127309187
1106	1	-0.07856019982726892
1106	2	-0.08243133515042445
1106	3	-0.04682957175370106
1106	4	-0.0370695456517538
1106	5	-0.01686150475974557
1106	6	-0.018407853273323838
1106	7	-0.05501123997068191
1106	8	-0.056636076267785995
1106	9	-0.16142113489956955
1106	10-11	-0.12095104672722101
1106	12-13	-0.08546606417514369
1106	14-15	-0.07915930833610929
1106	16-17	-0.021991103305090576
1106	18-19	-0.12679236242406233
1106	20-21	-0.12086732231683328
1106	22-23	-0.08679067642937355
1106	24-25	-0.1072399579152048
1106	26-27	-0.11374197869238145
1106	28-29	-0.0628635268674671
1106	30-31	-0.05596904265705405
1106	32-33	-0.04518988952469272
1106	34-35	-0.012557050190160624
1106	36-37	-0.013845249407935967
1106	38-39	-0.03325211621990576
1106	40-41	-0.007036893652113463
1106	42-43	-0.001676753302948697
1106	44-45	-0.024447329775643567
1106	46-47	-0.08020800574483289
1106	48-49	0.0345368850539991
1106	50-51	0.06352420083670296
1106	52-53	0.021862134953490653
1106	54-55	-0.022309342683065836
1106	56-57	-0.058010240256930246
1106	58-59	0.03474826189649605
1106	60-61	0.06733276099922847
1106	62-63	0.07528822215338948
1106	64-65	0.13811370631593434
1106	66-67	0.06474751090210518
1106	68-69	0.03178627374936127
1106	70-71	-0.0341644697400838
1106	72-73	-0.08379433613179188
1106	74-75	-0.014261922577347264
1106	76-77	0.006874021472068392
1106	78-79	0.09104919390123811
1106	80-81	0.17808926825721727
1106	82-83	0.16957366455642386
1106	84-85	-0.06445670989177188
1106	86-87	-0.20521667545563105
1106	88-89	-0.17581839958461742
1106	90-91	-0.1472812779226853
1106	92-93	-0.19232258680104053
1106	94-95	-0.12638467438323708
1106	96-97	-0.18960959112929565
1106	98-99	-0.17349532379375354
1106	100	-0.10910118626507703
1107	1	-0.10102843349085333
1107	2	-0.1271589050004529
1107	3	-0.07931052012155959
1107	4	-0.07537398249687044
1107	5	-0.05061724400854217
1107	6	-0.05842815574123961
1107	7	-0.11149699422597337
1107	8	-0.12314680663371291
1107	9	-0.19257013132065026
1107	10-11	-0.09801632720765951
1107	12-13	-0.14566079009891553
1107	14-15	-0.153906316184937
1107	16-17	-0.40116997953722233
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2207	72-73	0.10193492136409432
2207	74-75	0.19967041315048206
2207	76-77	0.12422197268196555
2207	78-79	0.0640151602862069
2207	80-81	0.09964071839739574
2207	82-83	0.0035388860132385958
2207	84-85	0.22509133269765513
2207	86-87	0.17812633067371664
2207	88-89	0.2091710776341067
2207	90-91	0.3691205847503465
2207	92-93	0.2915304054918373
2207	94-95	0.305059072180061
2207	96-97	0.42800173300891586
2207	98-99	0.38289389238147997
2207	100	0.28679083900594016
2208	1	-0.32324352511697896
2208	2	-0.33561620484810106
2208	3	-0.35744092410472916
2208	4	-0.33870779717345556
2208	5	-0.45983134879253384
2208	6	-0.40345796801345557
2208	7	-0.41007778479007584
2208	8	-0.36540641573417076
2208	9	-0.37321153702181675
2208	10-11	-0.3614391058512467
2208	12-13	-0.34811724944944444
2208	14-15	-0.3746410382284253
2208	16-17	-0.4181156862525839
2208	18-19	-0.4124683724893785
2208	20-21	-0.39338401763320974
2208	22-23	-0.4678241010649131
2208	24-25	-0.4072471946781846
2208	26-27	-0.3573435947404775
2208	28-29	-0.35244500696359893
2208	30-31	-0.2966644327031105
2208	32-33	-0.2639636912297618
2208	34-35	-0.2856263741110254
2208	36-37	-0.2737397844786713
2208	38-39	-0.1916617370810627
2208	40-41	-0.18008876227934678
2208	42-43	-0.12067167334233986
2208	44-45	-0.056103934049094306
2208	46-47	0.012387568749879563
2208	48-49	0.0021114245419759925
2208	50-51	0.0271163081453345
2208	52-53	0.09410800051252366
2208	54-55	0.12890174558825507
2208	56-57	0.3437374245356608
2208	58-59	0.38904379341098405
2208	60-61	0.4379379075099301
2208	62-63	0.238158942751717
2208	64-65	0.4176329507484624
2208	66-67	0.46231160327177534
2208	68-69	0.7182406193832271
2208	70-71	0.8012961517465698
2208	72-73	0.9102580917740148
2208	74-75	0.9751194239760785
2208	76-77	0.8993704833390268
2208	78-79	1.0007314705313881
2208	80-81	1.1359500387963912
2208	82-83	1.187398871404266
2208	84-85	1.1750695465205183
2208	86-87	1.057988634819523
2208	88-89	1.0404000523465022
2208	90-91	0.5082658492599545
2208	92-93	0.47084188731000864
2208	94-95	0.7915305200324827
2208	96-97	0.9102528048777394
2208	98-99	0.7091810513836627
2208	100	0.38712446707568837
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	772026.0
3	415359.0
4	275293.0
5	217060.0
6	219593.0
7	247207.0
8	271479.0
9	298703.0
10	323277.0
11	353666.0
12	406738.0
13	500266.0
14	671377.0
15	801764.0
16	876036.0
17	859564.0
18	835007.0
19	794294.0
20	788357.0
21	818687.0
22	854113.0
23	892804.0
24	932934.0
25	975210.0
26	1024563.0
27	1090942.0
28	1185384.0
29	1281634.0
30	1406877.0
31	1561285.0
32	1772198.0
33	2076873.0
34	2570853.0
35	3364886.0
36	4001434.0
37	2790004.0
38	531800.0
39	6326.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.29854272472492	28.207049657150645	27.100708001187655	22.39369961693678
2	23.655268115984505	26.481993631628303	28.409151383868984	21.453586868518208
3	23.889619674987674	26.684320100309712	26.21029587732232	23.215764347380297
4	24.794634861023532	24.58366091884228	27.282857142272015	23.33884707786217
5	25.97555756368196	24.873381349871963	25.022052859362613	24.129008227083464
6	26.141829376235258	24.75942283017726	25.264528476439462	23.834219317148015
7	25.21394856658203	25.94015749034324	24.650258617340544	24.195635325734187
8	25.66748409537717	24.87339777171038	25.61110486016386	23.8480132727486
9	24.98797594912436	25.525235147696645	24.978608927113576	24.50817997606542
10-11	25.424070777998647	24.84706874343471	25.32210376751137	24.406756711055277
12-13	24.576446049806567	24.892644714178047	25.62402713374749	24.906882102267893
14-15	23.901238030281842	25.144953374973895	26.923869626102224	24.02993896864204
16-17	23.49931818097181	25.16044995047679	27.504099325346527	23.83613254320488
18-19	22.803890307158934	25.1362158277498	29.00418248733279	23.05571137775847
20-21	21.86851708715089	25.070853125970466	30.801908475943645	22.25872131093499
22-23	21.749231183302918	24.938696460139912	31.460231168136094	21.85184118842108
24-25	21.234085290384762	24.387409827640504	32.63309825670081	21.74540662527392
26-27	21.24536958903331	24.41406046179731	33.5502348343647	20.790335114804684
28-29	21.28110266868429	24.474684178038554	33.51006436026915	20.734148793008004
30-31	21.29076152836414	24.424825476773744	33.47253208267203	20.811880912190087
32-33	21.294957426776044	24.503753537403774	33.35156354518875	20.849725490631435
34-35	21.391008514063543	24.562202785447237	33.29273539531391	20.754053305175308
36-37	21.46880653262539	24.607054353091243	32.98982373399197	20.934315380291395
38-39	21.588101819321555	24.782617930600644	32.57397277666692	21.055307473410878
40-41	21.717177852790094	25.03818332637525	32.06534492006882	21.17929390076584
42-43	21.923427529626252	25.26607289248109	31.364834312422683	21.445665265469973
44-45	22.08079133774573	25.618416441876914	30.66242737812758	21.63836484224978
46-47	22.335919535881953	25.934155430263672	29.98097156317042	21.748953470683958
48-49	22.472115820685815	26.20308313988196	29.30052698177736	22.024274057654864
50-51	22.56130670992184	26.57797596893627	28.688588104539097	22.172129216602794
52-53	22.68046639055543	26.834929217655507	28.30181895721579	22.182785434573272
54-55	22.84944242593649	26.922087210551528	27.98460518538298	22.243865178129006
56-57	22.8477589768437	27.116039798268798	27.77044541848759	22.265755806399916
58-59	22.94202826605693	27.306849215124394	27.60413221532155	22.146990303497127
60-61	22.93322052253751	27.436786960996734	27.362096847533568	22.267895668932187
62-63	22.97962869236356	27.663240701894953	27.146625769072102	22.210504836669383
64-65	23.02796323496355	27.93297081231458	26.86279926184834	22.17626669087353
66-67	23.046750243081355	28.139653625075866	26.541565664515183	22.272030467327596
68-69	23.007499281408027	28.393809080656986	26.28354569432239	22.315145943612595
70-71	23.05898701269284	28.60613910906483	26.043044420131174	22.29182945811116
72-73	23.076774220364094	28.76076183195663	25.69961139070408	22.462852556975204
74-75	23.206418873296506	28.841922933829693	25.50887140034936	22.44278679252444
76-77	22.42726228922817	29.591674374767248	25.112885654118028	22.868177681886547
78-79	22.724640085760726	29.597298939381705	24.942421012107747	22.735639962749826
80-81	22.699055728563412	29.710936485216994	24.659820115885218	22.93018767033438
82-83	22.641309408818984	29.890831616114983	24.45711046786946	23.010748507196574
84-85	22.716433383846656	29.922875880570114	24.19711715678244	23.163573578800786
86-87	22.717331008664406	30.012311716068506	23.979087041322725	23.291270233944363
88-89	22.776817113313246	30.04213207484262	23.6575788682914	23.523471943552735
90-91	22.715162088154248	30.07568250905937	23.49362505099894	23.715530351787443
92-93	22.64582211906794	30.096374304088396	23.255508267336594	24.00229530950707
94-95	22.740912200933415	30.138614746413335	23.062883435229477	24.057589617423773
96-97	22.526110666197045	30.188329519043695	22.92854276524358	24.35701704951568
98-99	22.356803630168006	30.157912902211205	22.78196468001511	24.703318787605678
100	23.43089036938002	30.0104707780053	22.23552198319428	24.3231168694204
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9502.0
1	7096.0
2	4371.5
3	4070.0
4	4454.5
5	5220.0
6	6485.5
7	8285.5
8	10850.5
9	14376.0
10	18686.5
11	24139.5
12	30005.0
13	38903.5
14	56687.0
15	77940.5
16	147488.0
17	190146.0
18	165479.5
19	153533.0
20	148260.0
21	149763.5
22	157061.5
23	168410.5
24	182148.5
25	198499.0
26	218064.0
27	243442.0
28	275819.5
29	312517.0
30	353182.5
31	403736.5
32	469236.5
33	540852.5
34	614179.5
35	705815.0
36	813071.0
37	931039.0
38	1047878.0
39	1132019.0
40	1200213.0
41	1296039.0
42	1437707.5
43	1564741.0
44	1648640.5
45	1690273.0
46	1659327.5
47	1590194.0
48	1530785.5
49	1492713.5
50	1434993.5
51	1346232.5
52	1239237.5
53	1116616.0
54	1010513.0
55	918871.5
56	821780.5
57	732478.5
58	662255.5
59	602512.5
60	544524.0
61	485541.0
62	428994.5
63	378798.5
64	334492.0
65	293949.5
66	256582.0
67	221333.0
68	188385.5
69	159270.0
70	133317.0
71	111043.0
72	92981.0
73	78322.0
74	65292.5
75	53754.0
76	44228.0
77	36178.5
78	29299.0
79	23431.0
80	18634.0
81	14593.5
82	11209.5
83	8380.5
84	6003.5
85	4190.0
86	2919.0
87	2056.5
88	1406.5
89	938.0
90	627.5
91	426.0
92	293.5
93	184.5
94	99.5
95	57.0
96	28.5
97	13.5
98	5.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05345330437131151
2	0.0
3	0.05221949090962334
4	0.005838855821806413
5	0.18736814098586763
6	0.16872271099637273
7	0.30943120098711224
8	0.33162448462370214
9	0.33637287460592524
10-11	0.31581528972871026
12-13	0.3139172136253041
14-15	0.31155709741850646
16-17	0.3317166366664838
18-19	0.37824189926588864
20-21	0.4521132805607595
22-23	0.4228319689668781
24-25	0.4238469213269597
26-27	0.4110467466066866
28-29	0.4772759077980927
30-31	0.43344993211850147
32-33	0.3902306240538897
34-35	0.4210119661219397
36-37	0.37410017689864505
38-39	0.39457072929101056
40-41	0.44094880459986135
42-43	0.4396113200900438
44-45	0.4372729620044585
46-47	0.43955628484227144
48-49	0.36626597337271843
50-51	0.4663942874129551
52-53	0.4644040592667672
54-55	0.47386372243620406
56-57	0.45306935800461956
58-59	0.42854795539830887
60-61	0.41415943782953474
62-63	0.4635977288924274
64-65	0.44366984964088735
66-67	0.4389688155695381
68-69	0.4717186276625637
70-71	0.44071074515600867
72-73	0.2615799728832375
74-75	0.6548477746804736
76-77	0.4243588771201913
78-79	0.45839113847526197
80-81	0.2532965281487502
82-83	7.551347950166122E-5
84-85	6.988196577611359E-4
86-87	6.105072834286846E-4
88-89	7.80732584678192E-5
90-91	2.802957967943018E-4
92-93	3.007740285235658E-4
94-95	2.969343600743288E-4
96-97	7.077788841426889E-4
98-99	0.0020324644991294577
100	2.0478231729264056E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.9065873E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.191654951424574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.36507780624818	35.574840206547094
2	9.381780926810526	8.10429289241313
3	3.225894535734298	4.179951710913653
4	1.4611262007418782	2.524338348117165
5	0.7990295942919178	1.7255705266316639
6	0.5119427470861382	1.3266992692217339
7	0.3533036899400677	1.0681839748269548
8	0.23263645639451636	0.8038362842971264
9	0.17424513617370951	0.6773342218720965
>10	1.2061291932602891	10.215783024750156
>50	0.12212473162560401	3.651394229807613
>100	0.1228228298958154	12.841869416935644
>500	0.03922535260648363	11.395468760369026
>1k	0.004448029571921934	2.432364170508197
>5k	4.72821378243522E-5	0.159694447840552
>10k+	1.6548748083596796E-4	3.31837851494836
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	215232	0.5509463464441202	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	202280	0.5177920892744416	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91152	0.23332897232323466	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	83682	0.21420742344603433	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77679	0.19884107031218784	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57146	0.14628112880006547	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51370	0.1314958454915368	No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45380	0.11616276948425036	No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39206	0.10035869414719081	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.0239115864632028E-5	0.0	0.0	0.0
2	0.0	1.2798894830790036E-5	0.0	0.0	0.0
3	2.559778966158007E-6	1.5358673796948044E-5	0.0	0.0	0.0
4	2.559778966158007E-6	2.0478231729264056E-5	0.0	0.0	0.0
5	5.119557932316014E-6	2.8157568627738078E-5	0.0	0.0	0.0
6	5.119557932316014E-6	3.071734759389609E-5	0.0	0.0	0.0
7	5.119557932316014E-6	3.327712656005409E-5	0.0	0.0	0.0
8	7.679336898474022E-6	4.351624242468612E-5	0.0	0.0	0.0
9	1.2798894830790036E-5	4.351624242468612E-5	0.0	0.0	0.0
10-11	2.4317900178501067E-5	5.1195579323160136E-5	0.0	2.559778966158007E-6	0.0
12-13	8.959226381553025E-5	5.6315137255476155E-5	0.0	3.839668449237011E-6	0.0
14-15	2.598175650650377E-4	7.42335900185822E-5	0.0	6.399447415395018E-6	0.0
16-17	6.245860677425536E-4	7.80732584678192E-5	2.559778966158007E-6	8.959226381553025E-6	0.0
18-19	0.001455234342260827	8.703248484937224E-5	2.559778966158007E-6	1.0239115864632028E-5	2.559778966158007E-6
20-21	0.002602015319099614	9.599171123092527E-5	2.559778966158007E-6	1.4078784313869039E-5	2.559778966158007E-6
22-23	0.00405852955084352	1.0111126916324128E-4	2.559778966158007E-6	1.6638563280027045E-5	2.559778966158007E-6
24-25	0.005820937369043308	1.0495093761247829E-4	2.559778966158007E-6	1.791845276310605E-5	2.559778966158007E-6
26-27	0.007697255351237126	1.075107165786363E-4	2.559778966158007E-6	1.791845276310605E-5	5.119557932316014E-6
28-29	0.011062084802251827	1.1646994296018931E-4	2.559778966158007E-6	1.791845276310605E-5	5.119557932316014E-6
30-31	0.013761371722065447	1.1774983244326832E-4	3.839668449237011E-6	1.791845276310605E-5	5.119557932316014E-6
32-33	0.014903033140971918	1.1902972192634733E-4	5.119557932316014E-6	1.791845276310605E-5	7.679336898474022E-6
34-35	0.01607925157592152	1.3438839572329537E-4	7.679336898474022E-6	2.0478231729264056E-5	7.679336898474022E-6
36-37	0.017418015975222162	1.407878431386904E-4	7.679336898474022E-6	2.0478231729264056E-5	7.679336898474022E-6
38-39	0.01888092965438146	1.420677326217694E-4	7.679336898474022E-6	2.0478231729264056E-5	7.679336898474022E-6
40-41	0.02049743007151024	1.459074010710064E-4	7.679336898474022E-6	2.3038010695422062E-5	7.679336898474022E-6
42-43	0.02227135689505774	1.5486662745255942E-4	7.679336898474022E-6	2.559778966158007E-5	8.959226381553025E-6
44-45	0.024292302388839487	1.6126607486795444E-4	8.959226381553025E-6	2.559778966158007E-5	1.4078784313869039E-5
46-47	0.026657538153569485	1.779046381479815E-4	1.4078784313869039E-5	3.071734759389609E-5	1.5358673796948044E-5
48-49	0.029615362748965063	1.8686386452953453E-4	1.791845276310605E-5	3.327712656005409E-5	1.5358673796948044E-5
50-51	0.03328992545488488	1.9454320142800853E-4	2.0478231729264056E-5	3.455701604313309E-5	1.6638563280027045E-5
52-53	0.037685065939778176	2.1630132264035157E-4	2.0478231729264056E-5	3.71167950092911E-5	1.791845276310605E-5
54-55	0.042752148403287955	2.2142088057266763E-4	2.3038010695422062E-5	3.967657397544911E-5	1.791845276310605E-5
56-57	0.04897241129105191	2.2526054902190462E-4	2.3038010695422062E-5	4.095646345852811E-5	1.791845276310605E-5
58-59	0.05665558785797517	2.3293988592037863E-4	2.559778966158007E-5	4.095646345852811E-5	1.791845276310605E-5
60-61	0.06611653091689516	2.4061922281885266E-4	2.559778966158007E-5	4.223635294160711E-5	1.791845276310605E-5
62-63	0.07713765925568847	2.4701867023424766E-4	2.559778966158007E-5	4.479613190776512E-5	1.9198342246185054E-5
64-65	0.09013877662480499	2.546980071327217E-4	2.559778966158007E-5	4.6076021390844124E-5	2.0478231729264056E-5
66-67	0.1058647787033967	2.585376755819587E-4	2.9437458110817082E-5	4.8635800357002133E-5	2.6877679144659076E-5
68-69	0.12418383687470648	2.610974545481167E-4	3.071734759389609E-5	5.119557932316014E-5	2.8157568627738078E-5
70-71	0.1456693416271537	2.6493712299735376E-4	3.327712656005409E-5	5.119557932316014E-5	2.9437458110817082E-5
72-73	0.17132856598392154	2.7261645989582774E-4	3.327712656005409E-5	5.119557932316014E-5	3.071734759389609E-5
74-75	0.20077114365267096	2.764561283450648E-4	3.327712656005409E-5	5.2475468806239144E-5	3.58369055262121E-5
76-77	0.2351732930683515	2.8413546524353877E-4	3.327712656005409E-5	5.5035247772397154E-5	3.58369055262121E-5
78-79	0.2740870529118855	2.8669524420969674E-4	3.83966844923701E-5	6.0154805704713166E-5	3.58369055262121E-5
80-81	0.31777992008523653	2.918148021420128E-4	3.83966844923701E-5	6.655425312010818E-5	3.71167950092911E-5
82-83	0.366890559440461	2.9437458110817083E-4	3.83966844923701E-5	7.42335900185822E-5	3.83966844923701E-5
84-85	0.4210887594909245	2.9437458110817083E-4	3.83966844923701E-5	7.42335900185822E-5	3.967657397544911E-5
86-87	0.48055114498529194	2.956544705912498E-4	3.83966844923701E-5	8.063303743397721E-5	4.351624242468612E-5
88	0.528945046229992	3.0461369697280284E-4	3.83966844923701E-5	8.447270588321423E-5	4.6076021390844124E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	52805	0.0	65.04083	1
TATCTCG	54850	0.0	61.006027	2
TATGGAT	63010	0.0	50.876667	2
ATATGGA	63775	0.0	50.648438	1
ATGGATC	64080	0.0	49.881046	3
ATCTCGA	70035	0.0	47.021317	3
GGATCCG	66795	0.0	46.033936	5
TGGATCC	72005	0.0	44.13154	4
TCTCGAG	78560	0.0	41.226414	4
CTCGAGG	89275	0.0	36.104713	5
GATCCGG	91530	0.0	33.466187	6
TCGAGGG	98920	0.0	32.466152	6
ATCCGGC	106780	0.0	28.078777	7
CGAGGGC	121110	0.0	26.051449	7
TCCGGCG	131800	0.0	22.630896	8
AGGGCGC	142275	0.0	21.94167	9
GAGGGCG	148545	0.0	21.116655	8
CCGGCGC	151320	0.0	19.509869	9
GCGCCGT	155835	0.0	14.035947	9
CGGCGCG	158840	0.0	13.855498	5
>>END_MODULE