FastQCFastQC Report
Wed 25 May 2016
SRR490995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27850586
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT818290.2938142845540126No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT619340.22237952192460153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA404840.14536139383207233No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301280.10817725702432258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG237900.050.8683362
ATGGATC320800.047.2378163
GGATCCG315350.046.9008185
ATATCTC285050.043.7800371
TGGATCC347250.043.537614
ATCTCGA282900.042.0794683
TATGGAT381000.039.9960672
TCTCGAG320900.036.5342144
ATATGGA425150.036.254591
CTCGAGG364400.032.0578735
GATCCGG465850.031.7891586
TCGAGGG434700.026.7654866
ATCCGGC586800.024.8287667
TCCGGCG818200.017.8469648
CGAGGGC657000.017.6449017
AATTCGT22850.016.6684441
GCCGGAT1302100.016.5620775
CGGATCC1309250.016.4644877
CCGGATC1313250.016.4071876
GATCCTT1346200.016.0757339
Produced by FastQC (version 0.11.5)