FastQCFastQC Report
Wed 25 May 2016
SRR490994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28690806
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT864060.30116267908263017No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT643820.22439941213223497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC258550.050.3579521
ATGGATC348550.047.1418573
TATCTCG271950.046.2423022
GGATCCG354800.044.9822045
TATGGAT383100.043.2203332
ATCTCGA298650.041.448683
TGGATCC396300.041.254334
ATATGGA426150.039.3577461
TCTCGAG335900.036.189864
GATCCGG508250.031.355066
CTCGAGG391250.030.9266785
TCGAGGG454150.026.5812846
ATCCGGC630050.024.5780417
CGAGGGC590950.020.1338837
TCCGGCG819250.018.8847248
AGGGCGC711500.016.5446939
CCGGCGC951400.016.0003369
GAGGGCG770850.015.3010568
CGGATCC1285750.013.7712456
GCCGGAT1286100.013.7708844
Produced by FastQC (version 0.11.5)