FastQCFastQC Report
Wed 25 May 2016
SRR490993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44585235
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT744420.16696558849583276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGATC349050.038.70673
GGATCCG349600.037.5789645
ATCTCGA257400.034.8367731
TGGATCC406550.033.076554
TATGGAT432900.031.4379332
GATCCGG542900.030.842381
TCTCGAG296500.030.061782
ATATGGA512600.027.0148431
CTCGAGG342800.025.8365653
ATCCGGC725000.025.8196281
ATATCTC298500.023.4870821
GCCGGAT1974250.022.7480414
CGGATCC1987750.022.5845036
CCGGATC1996350.022.468375
TATCTCG306200.022.1004942
GATCCTT2073650.021.6964788
CGCCGGA2092350.021.5866053
TCGAGGG418850.021.0970754
GCGCCGG2172450.020.9575842
GGATCCT2169950.020.7985257
Produced by FastQC (version 0.11.5)