FastQCFastQC Report
Wed 25 May 2016
SRR490992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43263115
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437680.10116701028115983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA341400.045.3135641
TCTCGAG404000.038.0664832
CTCGAGG511550.029.998943
TATCTCG155200.027.952512
TCGAGGG599350.025.5180724
GATCCGG560450.025.4279331
ATCCGGC657300.021.6522522
CGAGGGC816500.018.8466155
TCCGGCG757250.018.844013
ATATCTC236900.018.8138181
AGGGCGC890050.017.0858947
GCGCCGT839700.016.8511739
TATGGAT322650.016.75242
CGGCGCG874650.016.2448125
GGATCCG381700.016.1063252
GAGGGCG969700.015.8910146
GGGCGCG970550.015.7220058
CCGGCGC914600.015.5969014
ATGGATC394350.014.8347861
ATATGGA378950.014.4192391
Produced by FastQC (version 0.11.5)