##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR490992_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43263115 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.997289353760127 33.0 31.0 34.0 30.0 34.0 2 32.26741997195533 34.0 31.0 34.0 30.0 34.0 3 32.356247787520616 34.0 31.0 34.0 30.0 34.0 4 35.670674291483635 37.0 35.0 37.0 35.0 37.0 5 35.50280630509384 37.0 35.0 37.0 35.0 37.0 6 35.4564966022442 37.0 35.0 37.0 33.0 37.0 7 35.440881476056454 37.0 36.0 37.0 33.0 37.0 8 35.38313551855894 37.0 35.0 37.0 33.0 37.0 9 37.09152708953112 39.0 38.0 39.0 34.0 39.0 10-11 37.05545288405608 39.0 38.0 39.0 34.0 39.0 12-13 36.99668433491209 39.0 38.0 39.0 34.0 39.0 14-15 38.33094151912085 41.0 39.0 41.0 34.0 41.0 16-17 38.256919271763024 41.0 38.5 41.0 34.0 41.0 18-19 38.21089841773991 41.0 38.5 41.0 34.0 41.0 20-21 38.12216080603535 40.5 38.5 41.0 34.0 41.0 22-23 38.03376378469281 40.0 38.0 41.0 33.0 41.0 24-25 37.90895664817478 40.0 38.0 41.0 33.0 41.0 26-27 37.671084213422915 40.0 38.0 41.0 32.5 41.0 28-29 37.41977463943593 40.0 38.0 41.0 32.0 41.0 30-31 37.16404794245629 40.0 37.0 41.0 31.5 41.0 32-33 36.93418242075264 40.0 37.0 41.0 31.0 41.0 34-35 36.79680484172256 40.0 36.5 41.0 30.5 41.0 36-37 36.845084502121495 40.0 37.0 41.0 30.5 41.0 38-39 36.79195441659714 40.0 37.0 41.0 30.0 41.0 40-41 36.592167993451234 40.0 37.0 41.0 30.0 41.0 42-43 36.28304846981083 40.0 36.0 41.0 29.5 41.0 44-45 35.94045347867346 40.0 36.0 41.0 27.5 41.0 46-47 35.56250862888629 40.0 35.5 41.0 25.5 41.0 48-49 35.22123515609082 40.0 35.0 41.0 24.0 41.0 50-51 34.937959575957485 39.5 35.0 41.0 23.0 41.0 52-53 34.61580996883835 39.0 35.0 41.0 21.0 41.0 54-55 34.29053163416458 39.0 34.0 41.0 18.5 41.0 56-57 33.947925929512934 38.5 34.0 41.0 15.5 41.0 58-59 33.58521558144854 38.0 33.5 40.5 10.5 41.0 60-61 33.16989954375685 38.0 33.0 40.0 7.5 41.0 62-63 32.77250059317273 37.0 32.5 40.0 3.5 41.0 64-65 32.30700874174224 36.5 32.0 40.0 2.0 41.0 66-67 31.839163222528015 36.0 31.0 39.0 2.0 41.0 68-69 31.343371622685975 35.0 31.0 39.0 2.0 41.0 70-71 30.882315374193468 35.0 30.5 39.0 2.0 40.5 72-73 30.382605424967664 35.0 30.0 37.5 2.0 40.0 74-75 29.832434210990122 35.0 29.5 37.0 2.0 39.5 76-77 29.09285469851165 34.0 29.0 36.0 2.0 39.0 78-79 29.099196128156745 34.0 29.0 36.0 2.0 39.0 80-81 28.698749165426484 34.0 29.0 36.0 2.0 37.5 82-83 28.231465695431318 34.0 28.0 35.0 2.0 37.0 84-85 27.74846286496014 34.0 26.5 35.0 2.0 37.0 86-87 27.291412765816794 34.0 26.0 35.0 2.0 36.0 88-89 26.779092062603446 34.0 25.0 35.0 2.0 36.0 90-91 26.306366843903866 34.0 23.5 35.0 2.0 35.5 92-93 25.787866765950625 33.0 20.5 35.0 2.0 35.0 94-95 25.21436280073684 33.0 16.5 35.0 2.0 35.0 96-97 24.619808733143696 33.0 3.5 35.0 2.0 35.0 98-99 24.00160598699377 32.5 2.0 35.0 2.0 35.0 100 23.01299231458484 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.12832193046981288 1101 2 -0.06745788087171434 1101 3 -0.06658315413893945 1101 4 -0.03751350532023423 1101 5 -0.04877840631100128 1101 6 -0.053372465968351435 1101 7 -0.030955123259957418 1101 8 -0.03425634580634096 1101 9 -0.07932622046367754 1101 10-11 -0.10663443038909293 1101 12-13 -0.10272385551663632 1101 14-15 -0.1314629752942693 1101 16-17 -0.12245366779635702 1101 18-19 -0.17767192804733156 1101 20-21 -0.23225529698851943 1101 22-23 -0.2671525018751524 1101 24-25 -0.2415096067892435 1101 26-27 -0.23204067147684526 1101 28-29 -0.3041350093931996 1101 30-31 -0.3365656663016807 1101 32-33 -0.3633860673465463 1101 34-35 -0.3545893910867335 1101 36-37 -0.3736313942142857 1101 38-39 -0.45481981670888416 1101 40-41 -0.48484195603121805 1101 42-43 -0.5557317171808478 1101 44-45 -0.6259580251958567 1101 46-47 -0.8016665023966922 1101 48-49 -0.855385322256268 1101 50-51 -0.8562016869151989 1101 52-53 -0.900437269929192 1101 54-55 -0.9169090409918326 1101 56-57 -1.0128969437624065 1101 58-59 -1.018806879363261 1101 60-61 -1.085189660216308 1101 62-63 -1.1086532223842411 1101 64-65 -1.1365891671990127 1101 66-67 -1.2309114389354754 1101 68-69 -1.2532619001427747 1101 70-71 -1.2637306076348658 1101 72-73 -1.3617869323676821 1101 74-75 -1.391004084421521 1101 76-77 -1.2787117632921898 1101 78-79 -1.23448080962471 1101 80-81 -1.1983924011469576 1101 82-83 -1.1940671328568762 1101 84-85 -1.3238128327135534 1101 86-87 -1.4088851699522742 1101 88-89 -1.4397555308721302 1101 90-91 -1.4985628796743882 1101 92-93 -1.5765287720684569 1101 94-95 -1.729554291617486 1101 96-97 -1.7957304066696054 1101 98-99 -1.7742690229293565 1101 100 -1.7085259709728362 1102 1 -0.1015198366638721 1102 2 -0.028374658671758368 1102 3 -0.018898066603441066 1102 4 -0.006592381395066127 1102 5 0.009076431117897243 1102 6 0.0020253313813327622 1102 7 -0.03858910517715941 1102 8 -0.05370363089058827 1102 9 -0.036365811998038566 1102 10-11 -0.08508963950174575 1102 12-13 -0.032549032554094026 1102 14-15 -0.031093676978720453 1102 16-17 -0.040962317155099015 1102 18-19 -0.06274439992272818 1102 20-21 -0.06254330766370941 1102 22-23 -0.0732945508388596 1102 24-25 -0.12112414019497919 1102 26-27 -0.17687736447087588 1102 28-29 -0.2165157484408553 1102 30-31 -0.23728817055587115 1102 32-33 -0.2526450006198644 1102 34-35 -0.24053029051867725 1102 36-37 -0.20345035347988016 1102 38-39 -0.19789571882767376 1102 40-41 -0.24609815592333462 1102 42-43 -0.3206909808242173 1102 44-45 -0.33172595856993325 1102 46-47 -0.42097004301843555 1102 48-49 -0.4718644708364934 1102 50-51 -0.4629913658960092 1102 52-53 -0.5281118903873221 1102 54-55 -0.5771335595124469 1102 56-57 -0.5747717680278228 1102 58-59 -0.5164659156478635 1102 60-61 -0.6318601391056688 1102 62-63 -0.6320866229773259 1102 64-65 -0.7029563414993092 1102 66-67 -0.8016828572181822 1102 68-69 -0.8119412365132135 1102 70-71 -0.8459945109022691 1102 72-73 -0.8844633097233796 1102 74-75 -0.9076672313165268 1102 76-77 -0.8449419095428077 1102 78-79 -0.8804691072405681 1102 80-81 -0.9325771831428433 1102 82-83 -0.9458792817587529 1102 84-85 -1.0192789508472728 1102 86-87 -1.119325585863855 1102 88-89 -1.1300154845764148 1102 90-91 -1.212539290709767 1102 92-93 -1.3104507894036317 1102 94-95 -1.4269093370093522 1102 96-97 -1.5137090805747135 1102 98-99 -1.4678279991658734 1102 100 -1.4118348121300528 1103 1 0.07408353626238551 1103 2 0.10141064553566537 1103 3 0.0978366304774454 1103 4 0.09991627416374627 1103 5 0.10735494638321086 1103 6 0.1308456514391665 1103 7 0.14708050515460513 1103 8 0.11661575429364035 1103 9 0.13478188693353843 1103 10-11 0.11516425215479842 1103 12-13 0.14110948197907902 1103 14-15 0.19877058321149832 1103 16-17 0.23498796877859007 1103 18-19 0.2107705696919524 1103 20-21 0.19902097880119385 1103 22-23 0.1921113963470944 1103 24-25 0.20522057961535722 1103 26-27 0.1753739450734173 1103 28-29 0.1648385680812794 1103 30-31 0.10925765243415242 1103 32-33 0.15921041970623406 1103 34-35 0.17052377885850944 1103 36-37 0.1325003648132821 1103 38-39 0.18263473888622883 1103 40-41 0.16889892348355318 1103 42-43 0.15107367012004147 1103 44-45 0.1344643560123444 1103 46-47 0.06366218175923422 1103 48-49 0.054438645844300027 1103 50-51 0.03943019033625461 1103 52-53 0.02282036561761913 1103 54-55 0.0720059094932779 1103 56-57 -0.0317076261873126 1103 58-59 0.02988734566175566 1103 60-61 0.04498826832456615 1103 62-63 0.0249832612490124 1103 64-65 0.0036788231207367517 1103 66-67 -0.043890988728726654 1103 68-69 -0.03690743963665355 1103 70-71 -0.0718709972612821 1103 72-73 -0.10906091119639427 1103 74-75 -0.15931296686456164 1103 76-77 -0.15390530740053876 1103 78-79 -0.09501027018931651 1103 80-81 -0.032860559771243913 1103 82-83 -0.04968712176418322 1103 84-85 -0.16671157396609715 1103 86-87 -0.2779475793047652 1103 88-89 -0.29143635414744296 1103 90-91 -0.3385228605739101 1103 92-93 -0.3269686088132815 1103 94-95 -0.36625764691131124 1103 96-97 -0.3992168704285355 1103 98-99 -0.4066247238508147 1103 100 -0.39432165504458183 1104 1 0.041181926771173494 1104 2 0.06139621098004966 1104 3 0.05426685778952134 1104 4 0.05700349038806252 1104 5 0.04242187608054593 1104 6 0.06595417097433653 1104 7 0.08642296420619999 1104 8 0.07576655790251152 1104 9 0.04231336992017276 1104 10-11 0.06170844123790431 1104 12-13 0.1087568065256832 1104 14-15 0.16084869470410013 1104 16-17 0.13977757147405612 1104 18-19 0.12876633119964254 1104 20-21 0.1588619395492259 1104 22-23 0.0832797795666167 1104 24-25 0.10808611663962608 1104 26-27 0.11192158059072455 1104 28-29 0.13682963471813991 1104 30-31 0.15468072822078938 1104 32-33 0.1328441890979306 1104 34-35 0.1485353906249145 1104 36-37 0.16363416944737708 1104 38-39 0.22437057299516994 1104 40-41 0.24373521922845498 1104 42-43 0.1563597906815204 1104 44-45 0.1403650068150526 1104 46-47 0.13884706606032893 1104 48-49 0.16934750429429357 1104 50-51 0.1816826181606288 1104 52-53 0.15164789914339138 1104 54-55 0.17985977452685376 1104 56-57 0.1308944631789828 1104 58-59 0.1937319920779288 1104 60-61 0.09834980026825946 1104 62-63 0.0920966181241667 1104 64-65 0.11756269833541921 1104 66-67 0.14036123818108948 1104 68-69 0.18096022389008226 1104 70-71 0.09723617324206657 1104 72-73 0.06140676286405622 1104 74-75 0.08392771097479468 1104 76-77 0.06610760073769839 1104 78-79 0.08826111698164141 1104 80-81 0.16293239208306431 1104 82-83 0.13631058820779174 1104 84-85 0.05940004256309095 1104 86-87 0.007851964912767073 1104 88-89 0.023102853501491438 1104 90-91 0.030850712021507576 1104 92-93 0.0039944701352894185 1104 94-95 -0.03558992317639209 1104 96-97 -0.02374243357143513 1104 98-99 -0.01331557720888199 1104 100 0.036652725774246164 1105 1 0.04787370568104876 1105 2 0.07083481610150244 1105 3 0.05517401625329654 1105 4 0.03476565868909631 1105 5 0.021636671947803165 1105 6 0.06456572232007574 1105 7 0.06258897956013953 1105 8 0.06800003700085 1105 9 0.07299675476482292 1105 10-11 0.08663601709847057 1105 12-13 0.10380581770035491 1105 14-15 0.14669002901918304 1105 16-17 0.159672778100024 1105 18-19 0.1114627333491569 1105 20-21 -0.018841871341294336 1105 22-23 0.052410723869307674 1105 24-25 0.12570727637704238 1105 26-27 0.12152183116733539 1105 28-29 0.10956759147529738 1105 30-31 0.1429031824332796 1105 32-33 0.11763855268534229 1105 34-35 0.1630969040369692 1105 36-37 0.10976033202092594 1105 38-39 0.09949457190658251 1105 40-41 0.1456610127654585 1105 42-43 0.1705988229794997 1105 44-45 0.18928580214344493 1105 46-47 0.17192884414691179 1105 48-49 0.15866098817984664 1105 50-51 0.22445778839439612 1105 52-53 0.1819317035787762 1105 54-55 0.2083758854069515 1105 56-57 0.20658821122965065 1105 58-59 0.1705484424571324 1105 60-61 0.16973407507434501 1105 62-63 0.28128919811987174 1105 64-65 0.30773160158747714 1105 66-67 0.26186171210953546 1105 68-69 0.24594844429809015 1105 70-71 0.26898389535834966 1105 72-73 0.24446944668269666 1105 74-75 0.2782502928048416 1105 76-77 0.32167463984403 1105 78-79 0.3634788795625603 1105 80-81 0.36853468863227334 1105 82-83 0.3940774702932863 1105 84-85 0.3791176410964283 1105 86-87 0.31048063806020565 1105 88-89 0.339245339896344 1105 90-91 0.35847558624647036 1105 92-93 0.2445842396937259 1105 94-95 0.2468531627958832 1105 96-97 0.23754385654017796 1105 98-99 0.24146197881470144 1105 100 0.22694953960840536 1106 1 -0.05691544354096223 1106 2 -0.026804144287595477 1106 3 -0.03540604004277981 1106 4 -0.05726456000301283 1106 5 -0.06582623378231034 1106 6 -0.03617431287802475 1106 7 -0.017471987158820923 1106 8 -0.023035746184596917 1106 9 -0.020828860972152086 1106 10-11 -0.028669088988110047 1106 12-13 0.003660058489948881 1106 14-15 0.032774358395094794 1106 16-17 0.07062071906497636 1106 18-19 0.052551245182925754 1106 20-21 0.021459030945806035 1106 22-23 0.07437635842659773 1106 24-25 0.08288078375916541 1106 26-27 0.09071862784364271 1106 28-29 0.047765545301516 1106 30-31 0.10126439184482905 1106 32-33 0.127592161578427 1106 34-35 0.17235336546315239 1106 36-37 0.1601474008848669 1106 38-39 0.1808943996852932 1106 40-41 0.24830310767394792 1106 42-43 0.17421213720660234 1106 44-45 0.21274713530857525 1106 46-47 0.18964258886020247 1106 48-49 0.2191213554158864 1106 50-51 0.20019227545494545 1106 52-53 0.2166460385197908 1106 54-55 0.23258168227192044 1106 56-57 0.2330276923466741 1106 58-59 0.24803405131252987 1106 60-61 0.2767940515635914 1106 62-63 0.3562710906940936 1106 64-65 0.40468001666085485 1106 66-67 0.3719114887812438 1106 68-69 0.4507934038706125 1106 70-71 0.4348758725488757 1106 72-73 0.4121530904833719 1106 74-75 0.45258593217716836 1106 76-77 0.41689813844519463 1106 78-79 0.37230132063697496 1106 80-81 0.4439361050803754 1106 82-83 0.47558287807637 1106 84-85 0.4997344324818016 1106 86-87 0.4871665374621017 1106 88-89 0.5240713601916767 1106 90-91 0.5942144320230085 1106 92-93 0.5279174420009269 1106 94-95 0.5193437899670172 1106 96-97 0.5105792596040644 1106 98-99 0.5653158716339348 1106 100 0.5689660101144369 1107 1 -0.17367122677828917 1107 2 -0.14148321145395926 1107 3 -0.14338664667808132 1107 4 -0.15257847314068584 1107 5 -0.17402493668561902 1107 6 -0.17174262688968156 1107 7 -0.1693298660415934 1107 8 -0.18382953503320465 1107 9 -0.21946064202057158 1107 10-11 -0.21950063158591604 1107 12-13 -0.22118107172141066 1107 14-15 -0.2211115016936347 1107 16-17 -0.2297560203331983 1107 18-19 -0.27245818709784686 1107 20-21 -0.3488625962707488 1107 22-23 -0.3238675364784456 1107 24-25 -0.2256479331624064 1107 26-27 -0.2749861667170208 1107 28-29 -0.26201397719059116 1107 30-31 -0.3779119766469776 1107 32-33 -0.3662420837759299 1107 34-35 -0.38878989504449635 1107 36-37 -0.36656692691660453 1107 38-39 -0.2091682686996137 1107 40-41 -0.23789624904173934 1107 42-43 -0.23473932419605603 1107 44-45 -0.1667484996997075 1107 46-47 -0.2542443506783414 1107 48-49 -0.38353331885352304 1107 50-51 -0.34626109672282723 1107 52-53 -0.3866023341990612 1107 54-55 -0.37515686762079525 1107 56-57 -0.4171679414157623 1107 58-59 -0.28208581878232053 1107 60-61 -0.3357754822560324 1107 62-63 -0.38915173033615247 1107 64-65 -0.20210264954866375 1107 66-67 -0.1652526449981444 1107 68-69 -0.07060030878900392 1107 70-71 -0.03230472877143242 1107 72-73 -0.019834627289057494 1107 74-75 0.06405173948898124 1107 76-77 0.11243136489138905 1107 78-79 0.14028573386207555 1107 80-81 0.04846048171405215 1107 82-83 0.13931494300888403 1107 84-85 0.16563826138647642 1107 86-87 0.23268674885380491 1107 88-89 0.10962707626982393 1107 90-91 0.10560127682575171 1107 92-93 0.10049340163037712 1107 94-95 0.16962513892474007 1107 96-97 0.24136023445785426 1107 98-99 0.20245367197018993 1107 100 0.1771311834901148 1108 1 -0.24103970067558933 1108 2 -0.21228553957270435 1108 3 -0.20900149416314662 1108 4 -0.2155517768566284 1108 5 -0.2181466580242457 1108 6 -0.2434889319286384 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0.37760616750520626 2204 36-37 0.3944453413930731 2204 38-39 0.41013863352960556 2204 40-41 0.39415478005530247 2204 42-43 0.3636382967402838 2204 44-45 0.3781286080434896 2204 46-47 0.36661528934482845 2204 48-49 0.3884207484190938 2204 50-51 0.38256020350939224 2204 52-53 0.4063712225594074 2204 54-55 0.4125801419211328 2204 56-57 0.42807513560088495 2204 58-59 0.36901375656919555 2204 60-61 0.425685837561538 2204 62-63 0.3816732509022813 2204 64-65 0.409555232441285 2204 66-67 0.37028387838267207 2204 68-69 0.3517584706435244 2204 70-71 0.334505463361328 2204 72-73 0.3673615627711371 2204 74-75 0.41521979562626754 2204 76-77 0.3506166978013532 2204 78-79 0.3480444644040581 2204 80-81 0.31987741507779077 2204 82-83 0.3078412975126632 2204 84-85 0.2861381029967518 2204 86-87 0.23634496745346922 2204 88-89 0.23925470408471483 2204 90-91 0.2752116804558895 2204 92-93 0.24955376397278073 2204 94-95 0.2828033603467084 2204 96-97 0.2695553278883338 2204 98-99 0.31400330148319 2204 100 0.3929614843328757 2205 1 0.14847972828845712 2205 2 0.1372102754931248 2205 3 0.10759322270365601 2205 4 0.1060144197210775 2205 5 0.0942102774736071 2205 6 0.12045221833107433 2205 7 0.1282886199613742 2205 8 0.13384796343027716 2205 9 0.15607995876811032 2205 10-11 0.16978003404385333 2205 12-13 0.1363748827341098 2205 14-15 0.17313328423222174 2205 16-17 0.23956770582912412 2205 18-19 0.19780779325759568 2205 20-21 0.23974405032071644 2205 22-23 0.24421631686310974 2205 24-25 0.2308936045533443 2205 26-27 0.25255174190581187 2205 28-29 0.25399659254753004 2205 30-31 0.26504154161001026 2205 32-33 0.26312647040972337 2205 34-35 0.2994522948046594 2205 36-37 0.3685102048754487 2205 38-39 0.37888154709896327 2205 40-41 0.3638481665430078 2205 42-43 0.33711394175644216 2205 44-45 0.3333134290682338 2205 46-47 0.39303261594718464 2205 48-49 0.39104820334913626 2205 50-51 0.39100864284996106 2205 52-53 0.41741169747466955 2205 54-55 0.46415683934387175 2205 56-57 0.5032232634077545 2205 58-59 0.43837767876392064 2205 60-61 0.4745757962832613 2205 62-63 0.5145304735143128 2205 64-65 0.49527618741292656 2205 66-67 0.5166839517529311 2205 68-69 0.4836697284623952 2205 70-71 0.4713917754141512 2205 72-73 0.5184725373425394 2205 74-75 0.5190249097244894 2205 76-77 0.44058326012599736 2205 78-79 0.34434154408507567 2205 80-81 0.37256771104501496 2205 82-83 0.4341679571545214 2205 84-85 0.3824495141800739 2205 86-87 0.3663475637103275 2205 88-89 0.3774700397035389 2205 90-91 0.39244003495606705 2205 92-93 0.391596390782162 2205 94-95 0.43231679022536085 2205 96-97 0.2772259848362921 2205 98-99 0.3578534015671231 2205 100 0.4341831248556822 2206 1 0.03437803883680601 2206 2 0.0472049502350913 2206 3 0.013367069945445564 2206 4 0.013017079405074128 2206 5 -0.004482231520462676 2206 6 0.017353746124051384 2206 7 -0.007694549231729297 2206 8 0.013196446449015298 2206 9 0.005922750995765114 2206 10-11 0.020729576699658026 2206 12-13 0.024035763956128164 2206 14-15 0.028193240961890353 2206 16-17 0.06184506042641402 2206 18-19 0.02936605202911835 2206 20-21 0.051852288940679614 2206 22-23 0.0731151967940562 2206 24-25 0.03488069093751989 2206 26-27 0.1162163337125861 2206 28-29 0.09962933571985388 2206 30-31 0.14187695658187494 2206 32-33 0.13963795498941778 2206 34-35 0.1631758819796829 2206 36-37 0.24937786646883353 2206 38-39 0.19922498953844325 2206 40-41 0.23418238912361744 2206 42-43 0.21929466154934119 2206 44-45 0.26857557369422125 2206 46-47 0.29813757794027396 2206 48-49 0.31651489186020854 2206 50-51 0.30639868193658515 2206 52-53 0.3495187669857671 2206 54-55 0.3463451136424922 2206 56-57 0.3438164230970244 2206 58-59 0.36852975346621264 2206 60-61 0.4759003745662156 2206 62-63 0.45363693881780875 2206 64-65 0.4004758653248146 2206 66-67 0.4607552206306238 2206 68-69 0.3400023774553631 2206 70-71 0.3886073301102577 2206 72-73 0.4423921734368257 2206 74-75 0.46551332964061487 2206 76-77 0.35565258436659164 2206 78-79 0.37913709086888403 2206 80-81 0.4471657022072506 2206 82-83 0.45781514318263206 2206 84-85 0.48041616152756816 2206 86-87 0.5001093568448844 2206 88-89 0.4794649654852563 2206 90-91 0.488724372202352 2206 92-93 0.5237069760871336 2206 94-95 0.579102263204021 2206 96-97 0.5376991284033679 2206 98-99 0.6239289703380528 2206 100 0.6394354074285715 2207 1 -0.07116595064622544 2207 2 -0.09078539059756707 2207 3 -0.1010830958140545 2207 4 -0.07730188943672545 2207 5 -0.08907598093425406 2207 6 -0.07504375072948477 2207 7 -0.12609208858651755 2207 8 -0.10938427157067565 2207 9 -0.11686978297304762 2207 10-11 -0.11605141074735315 2207 12-13 -0.11260671981880677 2207 14-15 -0.13542455857143665 2207 16-17 -0.07575221719817904 2207 18-19 -0.1249315593996343 2207 20-21 -0.10869550202311729 2207 22-23 -0.10848971674807473 2207 24-25 -0.14792373898875155 2207 26-27 -0.11161667770725359 2207 28-29 -0.10147685386165506 2207 30-31 -0.09544645563490661 2207 32-33 -0.16825411782274102 2207 34-35 -0.13527838408850812 2207 36-37 -0.07550089350097977 2207 38-39 -0.15653220818840907 2207 40-41 -0.03472676553572285 2207 42-43 -0.0684807122385962 2207 44-45 -0.12691060861992298 2207 46-47 -0.11643648892576408 2207 48-49 -0.09675999047930617 2207 50-51 -0.13084975006059096 2207 52-53 -0.09904923246994457 2207 54-55 -0.061368428489316784 2207 56-57 -0.0695041720816647 2207 58-59 0.06767604649316894 2207 60-61 0.17106295413938 2207 62-63 0.1804192657725423 2207 64-65 0.15134923929004174 2207 66-67 0.10543798110994373 2207 68-69 0.12636607642901865 2207 70-71 0.09594592049792183 2207 72-73 0.21262931363545334 2207 74-75 0.2176872009481734 2207 76-77 0.1341828540353056 2207 78-79 0.2064828367987417 2207 80-81 0.11270440716323549 2207 82-83 0.06212358477835167 2207 84-85 0.19186854651493945 2207 86-87 0.2371114129987646 2207 88-89 0.32600875080763814 2207 90-91 0.37850821025928383 2207 92-93 0.44791496852162993 2207 94-95 0.3199790452092124 2207 96-97 0.44690969576718587 2207 98-99 0.48713657897760143 2207 100 0.5949297876732516 2208 1 -0.23648520520401206 2208 2 -0.24560149778142915 2208 3 -0.26249819137954944 2208 4 -0.21735774925843998 2208 5 -0.2704064787950671 2208 6 -0.27574351493612426 2208 7 -0.29911655462987596 2208 8 -0.3048811752127065 2208 9 -0.3646139595381044 2208 10-11 -0.36701707036489495 2208 12-13 -0.38176996285235987 2208 14-15 -0.6213055739783897 2208 16-17 -0.6545942392334965 2208 18-19 -0.46475842050323024 2208 20-21 -0.5088415425206918 2208 22-23 -0.390881226518097 2208 24-25 -0.4259655690808728 2208 26-27 -0.2728186661530856 2208 28-29 -0.3206380192019296 2208 30-31 -0.3379029317155826 2208 32-33 -0.34143353405134746 2208 34-35 -0.5774982657140981 2208 36-37 -0.5210581944972006 2208 38-39 -0.6219529383271833 2208 40-41 -0.44928681994595365 2208 42-43 -0.21461593366719 2208 44-45 -0.1011893523779861 2208 46-47 -0.06007193944014233 2208 48-49 -0.03353303960538767 2208 50-51 -0.03409380996833278 2208 52-53 0.04116941396110718 2208 54-55 0.12229303724530638 2208 56-57 0.15799381119855838 2208 58-59 -0.15875732505562468 2208 60-61 -0.3442962563186285 2208 62-63 0.13011816536371157 2208 64-65 0.26474135848344815 2208 66-67 0.3318826450579806 2208 68-69 0.3804660470176735 2208 70-71 0.41883735347440876 2208 72-73 0.5945871854910934 2208 74-75 0.6117954222864732 2208 76-77 0.576517493370762 2208 78-79 0.6419394618324041 2208 80-81 0.7479616499642248 2208 82-83 0.8789807399966314 2208 84-85 0.9983853821452833 2208 86-87 0.993813711145286 2208 88-89 1.0702955480550713 2208 90-91 1.1920845405475085 2208 92-93 1.326374002558559 2208 94-95 1.4692624531368388 2208 96-97 1.5710610839055974 2208 98-99 1.7907000934198116 2208 100 1.6670980660273855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 488893.0 3 157079.0 4 102350.0 5 86986.0 6 85204.0 7 93638.0 8 101263.0 9 110456.0 10 122968.0 11 133221.0 12 147546.0 13 180414.0 14 252264.0 15 372534.0 16 505648.0 17 500397.0 18 433350.0 19 344890.0 20 319382.0 21 327532.0 22 346293.0 23 373955.0 24 411829.0 25 457105.0 26 518493.0 27 586705.0 28 676410.0 29 785202.0 30 918698.0 31 1088783.0 32 1316261.0 33 1646932.0 34 2166039.0 35 3046311.0 36 4679324.0 37 7708150.0 38 9152241.0 39 2512836.0 40 5533.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.602930837222367 28.014717152341166 27.781718954448543 22.600633055987927 2 24.016566999394286 27.274866823620997 27.150187867887 21.558378309097716 3 23.62866381674089 25.97957867804942 26.872863870296904 23.51889363491279 4 24.367177444342598 25.79678324133618 26.64686072651033 23.189178587810886 5 24.770789476227154 26.006063579413052 26.160480355553435 23.062666588806362 6 24.805085061923293 25.843609733443007 25.656240553355897 23.695064651277807 7 24.631145579101403 26.308592378936762 25.81623482068575 23.244027221276088 8 24.21721135891394 25.94980042468047 26.106039752338685 23.726948464066908 9 24.06531060003423 25.721058227083276 26.227394860494908 23.986236312387586 10-11 23.938965097635712 25.947576821502565 26.20340213597657 23.91005594488515 12-13 23.612728877685498 26.016904039324146 26.414590598547495 23.955776484442865 14-15 23.322309315914953 26.267397759037927 26.88000852458266 23.53028440046446 16-17 23.103267067107858 26.19971076978623 27.124994351423844 23.572027811682077 18-19 22.65014849891229 26.19741069710162 27.910935162395802 23.241505641590297 20-21 22.313850955947114 25.9067267810004 28.784842469156462 22.994579793896026 22-23 22.26145412784077 25.861838658635655 29.234313109446695 22.642394104076878 24-25 22.18232436568657 25.7013023680796 29.72249455454144 22.3938787116924 26-27 22.227872403547455 25.73833506903051 29.798031186612427 22.235761340809603 28-29 22.26045173563387 25.83433691151239 29.73577595149272 22.169435401361024 30-31 22.247383122892707 25.769851559225522 29.705379730765095 22.277385587116676 32-33 22.282861635310987 25.81345849024658 29.617169626950496 22.28651024749194 34-35 22.32320946217657 25.911826753376253 29.597897737891554 22.167066046555618 36-37 22.295733523663294 25.868021495956366 29.548369189841132 22.287875790539207 38-39 22.315483986763322 25.926295413541073 29.465363277702032 22.292857321993573 40-41 22.420009557335067 26.09700861514659 29.201723288198494 22.281258539319854 42-43 22.556164760674307 26.217007258954883 28.73766949051172 22.489158489859086 44-45 22.65515092937714 26.45682817799874 28.27795340210708 22.61006749051704 46-47 22.87226256155831 26.707433364588017 27.75437445225805 22.66592962159562 48-49 22.953442398565223 26.78769237660927 27.358251166733723 22.90061405809178 50-51 22.985181487740768 26.914134592481375 27.14150957461107 22.959174345166776 52-53 23.059814347626148 26.99212712723067 27.015402150307484 22.932656374835698 54-55 23.085306576831098 26.963907200113912 26.92122467075624 23.029561552298755 56-57 23.050841616210924 27.018927900348594 26.934946749045647 22.99528373439484 58-59 23.10503338814925 27.077136955048687 26.912342475899514 22.905487180902554 60-61 23.054816590277714 27.061833919095196 26.863388591096893 23.0199608995302 62-63 23.077777983010563 27.109572707730173 26.87438248595092 22.938266823308343 64-65 23.136836843244467 27.24901654314897 26.755787217080247 22.85835939652632 66-67 23.157530675779984 27.311686863162077 26.615436783856467 22.915345677201472 68-69 23.16199498039601 27.42539198564287 26.517084122854932 22.895528911106183 70-71 23.23284906604389 27.565592862385728 26.397166320523702 22.804391751046683 72-73 23.229446560579 27.617230718275536 26.269898025630972 22.88342469551449 74-75 23.243177304135035 27.735914202731188 26.22728144515773 22.79362704797605 76-77 23.111526803799023 27.933845918246465 26.077388186275783 22.87723909167873 78-79 23.138069315731375 28.01628422149584 25.96153093771733 22.884115525055453 80-81 23.12433122944118 28.119693981017868 25.86664926140545 22.8893255281355 82-83 23.133303394757963 28.256530931614147 25.771134078197566 22.839031595430317 84-85 23.135613738332655 28.283257181448235 25.64178374233072 22.93934533788839 86-87 23.14261982210328 28.40338550641451 25.56142498272363 22.89256968875858 88-89 23.206524403776754 28.521163022090988 25.38342577083967 22.88888680329259 90-91 23.193644716605466 28.5735155011177 25.257999076844012 22.974840705432822 92-93 23.196297614589014 28.688113987031006 25.156689798889936 22.958898599490045 94-95 23.257178497291633 28.805735835700695 24.996606769365673 22.940478897642 96-97 23.236466773251564 28.873148198722227 24.831733754692483 23.058651273333727 98-99 23.25510166673316 28.97113334461646 24.734844945825035 23.038920042825342 100 23.64376838580696 28.95684547856302 24.115165758103913 23.2842203775261 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7146.0 1 4788.5 2 2216.5 3 2019.0 4 2128.0 5 2373.5 6 2762.5 7 3261.5 8 3784.5 9 4472.0 10 5383.5 11 6492.5 12 8180.5 13 11698.5 14 20405.5 15 28555.5 16 40087.5 17 45209.5 18 45165.5 19 56732.5 20 79150.0 21 95302.5 22 107639.0 23 131375.0 24 159765.5 25 194988.0 26 237096.0 27 287034.0 28 343551.0 29 402926.5 30 461311.5 31 522289.5 32 589649.5 33 665923.0 34 749677.5 35 844320.5 36 946268.0 37 1060092.0 38 1180327.5 39 1272297.0 40 1345430.0 41 1430336.0 42 1546698.5 43 1652276.0 44 1729542.0 45 1771068.5 46 1759013.5 47 1723487.0 48 1672279.5 49 1636347.5 50 1597287.0 51 1525233.0 52 1430250.5 53 1322956.0 54 1229658.0 55 1145103.0 56 1041931.0 57 935727.5 58 848744.5 59 769202.5 60 685768.0 61 602967.5 62 525950.0 63 457368.0 64 399712.5 65 343695.0 66 290999.0 67 244855.5 68 201700.5 69 162751.5 70 130551.0 71 105257.0 72 85226.5 73 68515.0 74 54365.0 75 42318.0 76 32362.5 77 24850.0 78 19312.5 79 14375.5 80 9791.0 81 6352.5 82 4108.5 83 2627.5 84 1629.5 85 1034.0 86 687.0 87 434.0 88 278.0 89 174.0 90 103.0 91 58.0 92 32.0 93 20.0 94 15.0 95 9.0 96 3.0 97 4.5 98 6.0 99 3.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1510663298285387 2 0.0 3 0.0 4 0.0 5 0.014231522626144695 6 9.176408125027521E-4 7 1.017032638542093E-4 8 0.0 9 0.0 10-11 0.0 12-13 5.431878864940724E-5 14-15 0.0 16-17 0.0 18-19 8.090032352039376E-6 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.4671567223025898E-6 30-31 4.622875629736786E-6 32-33 2.0802940333815537E-4 34-35 8.090032352039376E-6 36-37 3.4671567223025898E-6 38-39 0.0 40-41 2.080294033381554E-5 42-43 0.0 44-45 0.0 46-47 6.240882100144661E-4 48-49 8.124703919262401E-4 50-51 0.0 52-53 0.0 54-55 0.004287717146580869 56-57 0.0023726909169624055 58-59 0.004523483803697445 60-61 0.01762355761946406 62-63 0.013873249764840095 64-65 0.013295390311122998 66-67 0.02937144031353267 68-69 0.009994657111490932 70-71 0.005384494389735922 72-73 0.007907428764664772 74-75 9.9969685493058E-4 76-77 0.004599761251588102 78-79 0.007118072750887217 80-81 0.009625982780019422 82-83 0.013075803718710501 84-85 0.00509209750615507 86-87 0.0022894791556271436 88-89 0.007946723207517535 90-91 0.001511680330923929 92-93 2.034065277084186E-4 94-95 0.001176521847768012 96-97 0.0013313881813641945 98-99 0.0020363767148990543 100 0.0010725071460989345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.3263115E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.93346113300475 #Duplication Level Percentage of deduplicated Percentage of total 1 72.79944592995949 29.799332904782773 2 13.136825653150291 10.75471484568574 3 4.987048509150175 6.124114689531238 4 2.5153853814303715 4.118537189812336 5 1.4684836749280847 3.0055059716060364 6 0.9827320970209946 2.413597565853908 7 0.6576841948411762 1.8844903299125964 8 0.47331319216250406 1.549947772409781 9 0.35077670701583513 1.2922654232696469 >10 2.256942896653862 17.32417947293611 >50 0.2087368882577652 5.922559689062993 >100 0.15639044469500196 13.018295703044727 >500 0.005550135687555497 1.39792922176878 >1k 5.769303762682867E-4 0.430581380591291 >5k 5.65077213352489E-6 0.021402712858512404 >10k+ 1.0171389839139418E-4 0.9425451268737246 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43768 0.10116701028115983 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.311437814868393E-6 0.0 0.0 0.0 3 0.0 4.622875629736786E-6 0.0 0.0 0.0 4 0.0 4.622875629736786E-6 0.0 2.311437814868393E-6 2.311437814868393E-6 5 4.622875629736786E-6 6.9343134446051795E-6 0.0 2.311437814868393E-6 4.622875629736786E-6 6 4.622875629736786E-6 6.9343134446051795E-6 0.0 2.311437814868393E-6 9.245751259473573E-6 7 6.9343134446051795E-6 6.9343134446051795E-6 0.0 4.622875629736786E-6 9.245751259473573E-6 8 6.9343134446051795E-6 9.245751259473573E-6 0.0 4.622875629736786E-6 9.245751259473573E-6 9 9.245751259473573E-6 9.245751259473573E-6 0.0 4.622875629736786E-6 9.245751259473573E-6 10-11 3.35158483155917E-5 9.245751259473573E-6 0.0 1.040147016690777E-5 9.245751259473573E-6 12-13 1.4562058233670875E-4 1.1557189074341966E-5 0.0 1.7335783611512947E-5 1.1557189074341966E-5 14-15 5.29319259604862E-4 1.1557189074341966E-5 0.0 2.5425815963552325E-5 1.2712907981776162E-5 16-17 0.0011857675990274857 1.2712907981776162E-5 0.0 3.004869159328911E-5 1.5024345796644556E-5 18-19 0.002289479155627143 1.3868626889210359E-5 0.0 3.004869159328911E-5 1.8491502518947145E-5 20-21 0.003842765367218703 1.3868626889210359E-5 0.0 3.004869159328911E-5 1.9647221426381344E-5 22-23 0.005784373131708154 1.6180064704078752E-5 0.0 3.2360129408157504E-5 2.080294033381554E-5 24-25 0.008024156374315626 1.8491502518947145E-5 0.0 3.4671567223025894E-5 2.080294033381554E-5 26-27 0.010555180781596517 2.080294033381554E-5 0.0 3.813872394532848E-5 2.080294033381554E-5 28-29 0.013102385253581487 2.1958659241249733E-5 0.0 3.929444285276268E-5 2.080294033381554E-5 30-31 0.01544965035458034 2.3114378148683932E-5 0.0 3.929444285276268E-5 2.080294033381554E-5 32-33 0.017462912691330712 2.427009705611813E-5 2.311437814868393E-6 4.160588066763108E-5 2.3114378148683932E-5 34-35 0.019484265060433117 2.7737253778420718E-5 2.311437814868393E-6 4.160588066763108E-5 2.7737253778420718E-5 36-37 0.02155762478037007 3.35158483155917E-5 2.311437814868393E-6 4.160588066763108E-5 3.2360129408157504E-5 38-39 0.023484208199062873 3.813872394532848E-5 2.311437814868393E-6 4.391731848249947E-5 3.698300503789429E-5 40-41 0.025409635898848246 4.2761599575065275E-5 2.311437814868393E-6 4.391731848249947E-5 3.698300503789429E-5 42-43 0.027521134342730524 4.391731848249947E-5 2.311437814868393E-6 4.391731848249947E-5 3.698300503789429E-5 44-45 0.029816392092894835 4.391731848249947E-5 2.311437814868393E-6 4.7384475204802055E-5 3.929444285276268E-5 46-47 0.03255660162242131 4.5073037389933665E-5 3.4671567223025898E-6 5.085163192710465E-5 4.160588066763107E-5 48-49 0.035458611798988585 4.854019411223625E-5 4.622875629736786E-6 5.7785945371709826E-5 4.391731848249947E-5 50-51 0.03875356640408348 4.854019411223625E-5 4.622875629736786E-6 6.472025881631501E-5 4.391731848249947E-5 52-53 0.042752353823805794 5.431878864940724E-5 4.622875629736786E-6 6.472025881631501E-5 4.391731848249947E-5 54-55 0.04773465803375462 5.663022646427563E-5 4.622875629736786E-6 6.70316966311834E-5 4.5073037389933665E-5 56-57 0.05378831367089494 5.8941664279144024E-5 4.622875629736786E-6 7.0498853353486E-5 5.085163192710465E-5 58-59 0.06121611908897452 6.934313444605179E-5 6.9343134446051795E-6 7.627744789065696E-5 5.316306974197304E-5 60-61 0.07027117672872146 7.165457226092019E-5 6.9343134446051795E-6 7.858888570552536E-5 5.316306974197304E-5 62-63 0.08143195421781349 7.165457226092019E-5 6.9343134446051795E-6 7.858888570552536E-5 5.5474507556841436E-5 64-65 0.09548549613221333 7.165457226092019E-5 6.9343134446051795E-6 7.858888570552536E-5 5.5474507556841436E-5 66-67 0.11276233807944712 7.512172898322279E-5 6.9343134446051795E-6 8.321176133526215E-5 5.778594537170983E-5 68-69 0.13425061972537114 7.743316679809117E-5 8.090032352039376E-6 9.014607477986733E-5 6.240882100144661E-5 70-71 0.16037102275229143 7.858888570552536E-5 1.040147016690777E-5 9.361323150216992E-5 6.356453990888082E-5 72-73 0.19213826836093517 7.974460461295955E-5 1.1557189074341966E-5 9.476895040960411E-5 6.472025881631501E-5 74-75 0.23114493720574675 8.090032352039376E-5 1.3868626889210359E-5 1.0517042057651187E-4 6.70316966311834E-5 76-77 0.2784889622487886 8.090032352039376E-5 1.3868626889210359E-5 1.0863757729881448E-4 6.70316966311834E-5 78-79 0.3352624978575861 8.205604242782796E-5 1.3868626889210359E-5 1.1094901511368287E-4 6.70316966311834E-5 80-81 0.4024201678496798 8.552319915013055E-5 1.3868626889210359E-5 1.1210473402111706E-4 6.70316966311834E-5 82-83 0.48112578116485605 8.783463696499893E-5 1.5024345796644556E-5 1.1326045292855126E-4 6.70316966311834E-5 84-85 0.574422345686389 9.014607477986733E-5 1.7335783611512947E-5 1.1788332855828805E-4 6.70316966311834E-5 86-87 0.683077258768815 9.130179368730152E-5 1.8491502518947145E-5 1.2250620418802484E-4 6.70316966311834E-5 88 0.7758941999437627 9.245751259473573E-5 1.8491502518947145E-5 1.2481764200289322E-4 6.70316966311834E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGA 34140 0.0 45.313564 1 TCTCGAG 40400 0.0 38.066483 2 CTCGAGG 51155 0.0 29.99894 3 TATCTCG 15520 0.0 27.95251 2 TCGAGGG 59935 0.0 25.518072 4 GATCCGG 56045 0.0 25.427933 1 ATCCGGC 65730 0.0 21.652252 2 CGAGGGC 81650 0.0 18.846615 5 TCCGGCG 75725 0.0 18.84401 3 ATATCTC 23690 0.0 18.813818 1 AGGGCGC 89005 0.0 17.085894 7 GCGCCGT 83970 0.0 16.851173 9 TATGGAT 32265 0.0 16.7524 2 CGGCGCG 87465 0.0 16.244812 5 GGATCCG 38170 0.0 16.106325 2 GAGGGCG 96970 0.0 15.891014 6 GGGCGCG 97055 0.0 15.722005 8 CCGGCGC 91460 0.0 15.596901 4 ATGGATC 39435 0.0 14.834786 1 ATATGGA 37895 0.0 14.419239 1 >>END_MODULE