FastQCFastQC Report
Wed 25 May 2016
SRR490991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33545010
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT355590.10600384379077545No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342160.10200026770002453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA328300.049.2463951
TCTCGAG391750.041.2114832
TATCTCG133150.033.92212
CTCGAGG494850.032.5397573
TCGAGGG577850.027.865884
GATCCGG483850.025.43781
ATATCTC185950.024.8055291
ATCCGGC558500.022.098972
CGAGGGC762050.021.1919145
TCCGGCG649800.019.0011943
AGGGCGC848150.018.9382427
TATGGAT255200.017.9013632
GAGGGCG904350.017.8445936
GGGCGCG925000.017.4207178
ATGGATC329400.016.9607771
GCGCCGT747100.016.4487639
GGATCCG335950.016.2287043
CGGCGCG758400.016.2059275
CCGGCGC773700.015.9461994
ATATGGA304550.015.1764951
Produced by FastQC (version 0.11.5)