##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490991_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33545010
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.990512955578193	33.0	31.0	34.0	30.0	34.0
2	32.261265356605946	34.0	31.0	34.0	30.0	34.0
3	32.350595513311816	34.0	31.0	34.0	30.0	34.0
4	35.67113827063996	37.0	35.0	37.0	35.0	37.0
5	35.50717200561276	37.0	35.0	37.0	35.0	37.0
6	35.46121619877293	37.0	35.0	37.0	33.0	37.0
7	35.44629409858575	37.0	35.0	37.0	33.0	37.0
8	35.38925491451635	37.0	35.0	37.0	33.0	37.0
9	37.09527592926638	39.0	38.0	39.0	34.0	39.0
10-11	37.059441135954344	39.0	38.0	39.0	34.0	39.0
12-13	36.99945161739406	39.0	38.0	39.0	34.0	39.0
14-15	38.32505724398353	41.0	38.5	41.0	34.0	41.0
16-17	38.25329011677147	41.0	38.0	41.0	34.0	41.0
18-19	38.206952762869946	41.0	38.5	41.0	34.0	41.0
20-21	38.11948195871756	40.0	38.5	41.0	33.5	41.0
22-23	38.032480255632656	40.0	38.0	41.0	33.0	41.0
24-25	37.90542749875466	40.0	38.0	41.0	33.0	41.0
26-27	37.6535580403762	40.0	38.0	41.0	32.5	41.0
28-29	37.385762740866674	40.0	37.5	41.0	32.0	41.0
30-31	37.114082496919806	40.0	37.0	41.0	31.0	41.0
32-33	36.874688232318306	40.0	36.0	41.0	31.0	41.0
34-35	36.73234445301999	40.0	36.0	41.0	30.0	41.0
36-37	36.770674386443766	40.0	36.5	41.0	30.0	41.0
38-39	36.6959970797445	40.0	37.0	41.0	30.0	41.0
40-41	36.45881584176007	40.0	36.0	41.0	30.0	41.0
42-43	36.10041576973744	40.0	36.0	41.0	28.5	41.0
44-45	35.708951703994124	40.0	35.5	41.0	26.5	41.0
46-47	35.28265618343831	40.0	35.0	41.0	23.5	41.0
48-49	34.914346053258	39.5	35.0	41.0	22.5	41.0
50-51	34.619587697246175	39.0	35.0	41.0	19.5	41.0
52-53	34.28866786744139	39.0	34.5	41.0	16.5	41.0
54-55	33.95299952213459	39.0	34.0	41.0	12.5	41.0
56-57	33.601230555602754	38.5	33.5	41.0	8.0	41.0
58-59	33.23063085985069	38.0	33.0	40.0	4.5	41.0
60-61	32.80802000059025	37.5	33.0	40.0	2.0	41.0
62-63	32.40214784553649	37.0	32.0	40.0	2.0	41.0
64-65	31.92979204656669	36.0	31.5	40.0	2.0	41.0
66-67	31.456177222782166	36.0	31.0	39.0	2.0	41.0
68-69	30.95484113732564	35.0	30.5	39.0	2.0	41.0
70-71	30.48910407240898	35.0	30.0	38.5	2.0	40.0
72-73	29.98449699374065	35.0	29.5	37.5	2.0	40.0
74-75	29.43021462804751	35.0	29.0	37.0	2.0	39.0
76-77	28.699879997054705	34.0	28.5	36.0	2.0	39.0
78-79	28.706019732890226	34.0	29.0	36.0	2.0	39.0
80-81	28.307638557865985	34.0	27.5	35.5	2.0	37.0
82-83	27.8396902102578	34.0	26.5	35.0	2.0	37.0
84-85	27.35378892419469	34.0	26.0	35.0	2.0	36.5
86-87	26.89490345956075	34.0	25.0	35.0	2.0	36.0
88-89	26.3782671998011	34.0	24.0	35.0	2.0	36.0
90-91	25.89910661824218	33.0	21.5	35.0	2.0	35.5
92-93	25.373068006239976	33.0	18.5	35.0	2.0	35.0
94-95	24.789806293096945	33.0	6.0	35.0	2.0	35.0
96-97	24.185047865539467	32.5	2.0	35.0	2.0	35.0
98-99	23.555861095286602	32.0	2.0	35.0	2.0	35.0
100	22.57032542246969	31.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.11696711649819491
1101	2	-0.060303875431444
1101	3	-0.06284788469046276
1101	4	-0.025084514346417564
1101	5	-0.05217337034127212
1101	6	-0.07093290783014083
1101	7	-0.021364571507419328
1101	8	-0.029605723830307795
1101	9	-0.06971737328122884
1101	10-11	-0.11368908446871018
1101	12-13	-0.08132955682277299
1101	14-15	-0.12291978757700406
1101	16-17	-0.12989155855400014
1101	18-19	-0.16367384478989067
1101	20-21	-0.22273635667846747
1101	22-23	-0.24179338885047486
1101	24-25	-0.22154343628439932
1101	26-27	-0.21664197218220949
1101	28-29	-0.26531774974459665
1101	30-31	-0.2902887787030437
1101	32-33	-0.3318017330477332
1101	34-35	-0.3107279935536198
1101	36-37	-0.3447745159382478
1101	38-39	-0.43115919213987297
1101	40-41	-0.4832851789014043
1101	42-43	-0.5648565601078204
1101	44-45	-0.6444260003148514
1101	46-47	-0.7930569079687118
1101	48-49	-0.8218936139539252
1101	50-51	-0.822672954544835
1101	52-53	-0.8873093915386363
1101	54-55	-0.8970511777309866
1101	56-57	-0.9701734021507562
1101	58-59	-1.0006325037763872
1101	60-61	-1.0597408117198341
1101	62-63	-1.0950982101784952
1101	64-65	-1.1349968718461056
1101	66-67	-1.2308019871390705
1101	68-69	-1.2615868349481154
1101	70-71	-1.2890570623281583
1101	72-73	-1.338816256344149
1101	74-75	-1.3887188108334634
1101	76-77	-1.2405529839756824
1101	78-79	-1.1940529559165363
1101	80-81	-1.1907557654657133
1101	82-83	-1.1850720793008414
1101	84-85	-1.2916188243664095
1101	86-87	-1.3863691469018775
1101	88-89	-1.4206447360894963
1101	90-91	-1.4489487180363092
1101	92-93	-1.5580824652024745
1101	94-95	-1.713616714825882
1101	96-97	-1.7560088618825525
1101	98-99	-1.7158256672243013
1101	100	-1.6187757952595554
1102	1	-0.11263860332251596
1102	2	-0.029905641482692147
1102	3	-0.028658659369881434
1102	4	-6.035188281785508E-4
1102	5	-0.0010814921457154014
1102	6	0.0019394325680437419
1102	7	-0.05112786387019952
1102	8	-0.06050484297976766
1102	9	-0.04749768974894408
1102	10-11	-0.08380101080119573
1102	12-13	-0.037033192016259875
1102	14-15	-0.04627632361707157
1102	16-17	-0.051751846096870224
1102	18-19	-0.05836554544185191
1102	20-21	-0.09127110678917916
1102	22-23	-0.10580992043632165
1102	24-25	-0.14678843404342956
1102	26-27	-0.21370233435859376
1102	28-29	-0.26845162808421463
1102	30-31	-0.26166015441384616
1102	32-33	-0.26668444400640823
1102	34-35	-0.2770505258204281
1102	36-37	-0.2340410999133482
1102	38-39	-0.24676059232994163
1102	40-41	-0.32139166002794184
1102	42-43	-0.38387290971201793
1102	44-45	-0.4183397714737822
1102	46-47	-0.5213629846304073
1102	48-49	-0.5755729694253304
1102	50-51	-0.5698750980941725
1102	52-53	-0.6470261126102415
1102	54-55	-0.6488776650694845
1102	56-57	-0.635627197251317
1102	58-59	-0.6175171827499426
1102	60-61	-0.708328170186391
1102	62-63	-0.7087203404855735
1102	64-65	-0.7772412931959138
1102	66-67	-0.8879969819136058
1102	68-69	-0.9008273366011643
1102	70-71	-0.9476411263332309
1102	72-73	-0.974330153910433
1102	74-75	-0.9924957165079427
1102	76-77	-0.9468899816798668
1102	78-79	-0.9645673086239235
1102	80-81	-1.0175791988969465
1102	82-83	-1.0018675820084546
1102	84-85	-1.0929973858279993
1102	86-87	-1.188505437426226
1102	88-89	-1.2039726991411541
1102	90-91	-1.2634310179277506
1102	92-93	-1.3901181248310905
1102	94-95	-1.5150003810526371
1102	96-97	-1.6068655013361592
1102	98-99	-1.5669348758644652
1102	100	-1.505476822379773
1103	1	0.05838602843938645
1103	2	0.10180488048513325
1103	3	0.11018214031048501
1103	4	0.09153663544864088
1103	5	0.1086798259421613
1103	6	0.1406373044612934
1103	7	0.1399435258628685
1103	8	0.11884114487734365
1103	9	0.14397431277937045
1103	10-11	0.11512744249318274
1103	12-13	0.1427310033598772
1103	14-15	0.20692873642782672
1103	16-17	0.24932470819872066
1103	18-19	0.23424272418423442
1103	20-21	0.20222824401622574
1103	22-23	0.20285781833576522
1103	24-25	0.2127945745765416
1103	26-27	0.1904562975243138
1103	28-29	0.19255666187123666
1103	30-31	0.1448375916389537
1103	32-33	0.1827245290633428
1103	34-35	0.18833650184801343
1103	36-37	0.15009722748890653
1103	38-39	0.21950253739322534
1103	40-41	0.21742772211328543
1103	42-43	0.18288880938883523
1103	44-45	0.184910178532391
1103	46-47	0.1391762808502932
1103	48-49	0.1433017195126709
1103	50-51	0.10502274384663934
1103	52-53	0.10103316984346833
1103	54-55	0.12071869094201304
1103	56-57	0.04263890075122134
1103	58-59	0.12514752896846915
1103	60-61	0.14472813716721333
1103	62-63	0.10413003074863525
1103	64-65	0.06828455084686524
1103	66-67	7.453340933949448E-5
1103	68-69	0.040430744227709425
1103	70-71	-0.0062690831021612325
1103	72-73	-0.06606997346289134
1103	74-75	-0.09583904801156962
1103	76-77	-0.0802139046970538
1103	78-79	-0.039555580270093316
1103	80-81	0.018817932984728003
1103	82-83	0.039918804541233754
1103	84-85	-0.06757631609477244
1103	86-87	-0.216332794363705
1103	88-89	-0.2486521671593458
1103	90-91	-0.2732455135870211
1103	92-93	-0.2423094300393096
1103	94-95	-0.317166413777791
1103	96-97	-0.3741477777910305
1103	98-99	-0.357615360527447
1103	100	-0.34855398118542524
1104	1	0.021683748665722646
1104	2	0.03655501413508233
1104	3	0.03536015820473892
1104	4	0.04063082593135903
1104	5	0.041326137355234493
1104	6	0.05299098406453595
1104	7	0.08356082292710454
1104	8	0.06559759252127861
1104	9	0.03176305662439205
1104	10-11	0.05948051334795679
1104	12-13	0.09729835446774615
1104	14-15	0.15488233900490656
1104	16-17	0.1346552285534912
1104	18-19	0.12867290689266753
1104	20-21	0.16422100483261914
1104	22-23	0.06929266731953732
1104	24-25	0.09215069619527583
1104	26-27	0.09624347721894821
1104	28-29	0.11944865299273033
1104	30-31	0.14766268795274584
1104	32-33	0.13921513463143498
1104	34-35	0.14811499176870768
1104	36-37	0.16674847458976672
1104	38-39	0.20237809043279498
1104	40-41	0.22027140532561873
1104	42-43	0.11941357821562093
1104	44-45	0.13598284616121958
1104	46-47	0.13342235176497041
1104	48-49	0.17746294500370396
1104	50-51	0.21129745411094092
1104	52-53	0.17149063109039986
1104	54-55	0.19347165152942125
1104	56-57	0.12845814486482965
1104	58-59	0.18858200809192027
1104	60-61	0.06150557756236452
1104	62-63	0.07794094278816033
1104	64-65	0.09439646690230319
1104	66-67	0.09865542258560467
1104	68-69	0.14407378089303435
1104	70-71	0.06598587486646323
1104	72-73	0.06404117881595539
1104	74-75	0.09672230083848277
1104	76-77	0.07922637862441917
1104	78-79	0.10663393022953827
1104	80-81	0.1930731944850983
1104	82-83	0.16456223186817098
1104	84-85	0.08708190975636398
1104	86-87	0.05125269211656658
1104	88-89	0.06069919322753137
1104	90-91	0.0868077794042783
1104	92-93	0.026176973174692364
1104	94-95	-0.012306688517149666
1104	96-97	-3.966951235732097E-4
1104	98-99	-8.521076192522514E-4
1104	100	0.013558424063919716
1105	1	0.04300987498439923
1105	2	0.06976047785214234
1105	3	0.05260396921977417
1105	4	0.03708099273007548
1105	5	0.019485206194922
1105	6	0.05804385320410432
1105	7	0.06112284759763753
1105	8	0.07367301408388016
1105	9	0.07609265768292772
1105	10-11	0.08992886153166779
1105	12-13	0.09930259691503807
1105	14-15	0.14726306756454477
1105	16-17	0.16029313632196107
1105	18-19	0.11739731993195335
1105	20-21	-0.00619899561061743
1105	22-23	0.06571850870970053
1105	24-25	0.12394196547690228
1105	26-27	0.13882618417972736
1105	28-29	0.13342323457246152
1105	30-31	0.13773429586525765
1105	32-33	0.10156527189722198
1105	34-35	0.17086095975638216
1105	36-37	0.12716105025955216
1105	38-39	0.12170506033974249
1105	40-41	0.19595239552166532
1105	42-43	0.2124537018053232
1105	44-45	0.22214338491092178
1105	46-47	0.20302942045496053
1105	48-49	0.20107554848757303
1105	50-51	0.25367971143491275
1105	52-53	0.227850112102864
1105	54-55	0.2389055035113472
1105	56-57	0.24473070840702604
1105	58-59	0.20684442325907781
1105	60-61	0.1974040880571124
1105	62-63	0.31944345101928207
1105	64-65	0.3326574764066166
1105	66-67	0.3051771982019922
1105	68-69	0.2598854038830005
1105	70-71	0.267279385290891
1105	72-73	0.2284408285807622
1105	74-75	0.2756801483321887
1105	76-77	0.3365585777534008
1105	78-79	0.37500258713426504
1105	80-81	0.3788890121204851
1105	82-83	0.39896446962282184
1105	84-85	0.378981647559705
1105	86-87	0.34044030860325947
1105	88-89	0.35076073292389864
1105	90-91	0.35916127394899533
1105	92-93	0.2502461553016815
1105	94-95	0.23977338807332416
1105	96-97	0.22400245404118024
1105	98-99	0.22428125027213497
1105	100	0.2177139375951569
1106	1	-0.058349648510056085
1106	2	-0.022895913155274172
1106	3	-0.025419134052306447
1106	4	-0.039178642977589107
1106	5	-0.05962427004845239
1106	6	-0.028288023223026926
1106	7	-0.01501408737866683
1106	8	-0.004830958711103506
1106	9	-0.0338238995145872
1106	10-11	-0.042357077239472574
1106	12-13	0.009484667511351574
1106	14-15	0.04362761392454928
1106	16-17	0.10297485759094371
1106	18-19	0.07291851492604451
1106	20-21	0.04036607680710347
1106	22-23	0.07782345903438426
1106	24-25	0.08145289266751377
1106	26-27	0.07808213534924136
1106	28-29	0.052826762960457074
1106	30-31	0.10002885576867016
1106	32-33	0.1210589386296661
1106	34-35	0.16638751267612406
1106	36-37	0.15815983192900518
1106	38-39	0.16367019961114693
1106	40-41	0.22498644822079683
1106	42-43	0.18614751472038193
1106	44-45	0.21995815676778818
1106	46-47	0.199161204868318
1106	48-49	0.23091221099464576
1106	50-51	0.19756639094657658
1106	52-53	0.20567998238947638
1106	54-55	0.23022164940753953
1106	56-57	0.2433449680775226
1106	58-59	0.2458977957011328
1106	60-61	0.2425097373266425
1106	62-63	0.31440790049730083
1106	64-65	0.3602901864621657
1106	66-67	0.3686177859903772
1106	68-69	0.43387974120676276
1106	70-71	0.39089438635499363
1106	72-73	0.4095532203567309
1106	74-75	0.42623066326703807
1106	76-77	0.39563241089462053
1106	78-79	0.3681727584921646
1106	80-81	0.4303130615777526
1106	82-83	0.4738939512955831
1106	84-85	0.5003783008394649
1106	86-87	0.4627454115841054
1106	88-89	0.5074109167812715
1106	90-91	0.5304825975454044
1106	92-93	0.47948179393433676
1106	94-95	0.5130575436289391
1106	96-97	0.49731197510098113
1106	98-99	0.5398886823649036
1106	100	0.5196516564804661
1107	1	-0.18706024990981618
1107	2	-0.13755781916619014
1107	3	-0.15533458498841668
1107	4	-0.15799430293190397
1107	5	-0.16909381647977284
1107	6	-0.1692628166744612
1107	7	-0.1866218023723647
1107	8	-0.20443156166223275
1107	9	-0.23365840546838967
1107	10-11	-0.23516657146169706
1107	12-13	-0.21611900320201727
1107	14-15	-0.22564973856363935
1107	16-17	-0.23507821414083452
1107	18-19	-0.26962897895648297
1107	20-21	-0.35658737945735197
1107	22-23	-0.3243226308364413
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2207	82-83	-0.058490207564805274
2207	84-85	0.07591002287621507
2207	86-87	0.13848880486579773
2207	88-89	0.2180501264677872
2207	90-91	0.256270789647818
2207	92-93	0.31645918019869157
2207	94-95	0.19905890401077642
2207	96-97	0.3346595434543431
2207	98-99	0.39076417135347796
2207	100	0.4768219493199908
2208	1	-0.21719817115836193
2208	2	-0.23705667556462373
2208	3	-0.25227090628467863
2208	4	-0.2067072616698482
2208	5	-0.26198617754734244
2208	6	-0.2614089645873463
2208	7	-0.2885121500829584
2208	8	-0.3020098832667699
2208	9	-0.3554689023186697
2208	10-11	-0.3508984571879026
2208	12-13	-0.390266265568755
2208	14-15	-0.6316622188534069
2208	16-17	-0.6688408266007997
2208	18-19	-0.4840183242670335
2208	20-21	-0.5383830999949879
2208	22-23	-0.40670434315437376
2208	24-25	-0.44554178197664385
2208	26-27	-0.2804004942704523
2208	28-29	-0.30543501360409664
2208	30-31	-0.34752469332579494
2208	32-33	-0.35760341209039126
2208	34-35	-0.6038660948854613
2208	36-37	-0.5409021653494435
2208	38-39	-0.6297273537717913
2208	40-41	-0.4665302070241424
2208	42-43	-0.21285615633410515
2208	44-45	-0.0990398209414991
2208	46-47	-0.04880387033288258
2208	48-49	-0.022075274113753096
2208	50-51	-0.016986764836858015
2208	52-53	0.06853414877171105
2208	54-55	0.14185996378245846
2208	56-57	0.16727935640926717
2208	58-59	-0.1344076616662946
2208	60-61	-0.31731374986335226
2208	62-63	0.15365308610516593
2208	64-65	0.29869556001777653
2208	66-67	0.37549441774459424
2208	68-69	0.40176134852867307
2208	70-71	0.44022793308133146
2208	72-73	0.6263464657276963
2208	74-75	0.6488875939067036
2208	76-77	0.6015344731034027
2208	78-79	0.6662490914901618
2208	80-81	0.7951374003833038
2208	82-83	0.9232121427061166
2208	84-85	1.0455400471616052
2208	86-87	1.054661663013242
2208	88-89	1.1440569318599536
2208	90-91	1.2686378475567714
2208	92-93	1.405216966474697
2208	94-95	1.5451704109132223
2208	96-97	1.640768205195318
2208	98-99	1.84027019589859
2208	100	1.7314606532255397
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	362576.0
3	117621.0
4	78414.0
5	66727.0
6	65618.0
7	73871.0
8	81095.0
9	89090.0
10	99809.0
11	107916.0
12	119782.0
13	149892.0
14	217556.0
15	333500.0
16	454119.0
17	448505.0
18	377076.0
19	287409.0
20	261286.0
21	264549.0
22	280993.0
23	300333.0
24	328950.0
25	364596.0
26	412699.0
27	466262.0
28	537652.0
29	619220.0
30	727100.0
31	859756.0
32	1039376.0
33	1299401.0
34	1706259.0
35	2382667.0
36	3641440.0
37	5895706.0
38	6815431.0
39	1803873.0
40	6885.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.10317213294013	27.57381652399197	27.41111586320247	22.91189547986544
2	24.44410062778339	26.870011366817298	27.044961381737554	21.64092662366176
3	24.034149937650938	25.586658045414207	26.623870435572982	23.755321581361876
4	24.765635783086665	25.416555249201	26.47247086824538	23.345338099466957
5	25.097327622714676	25.650661573811867	25.85528988926881	23.396720914204643
6	25.313020909040716	25.424618190330023	25.393224281708203	23.869136618921054
7	25.02631541134826	25.980631022547573	25.51644833197406	23.476605234130098
8	24.717691245285067	25.515341924178887	25.791889762441567	23.975077068094482
9	24.537217308923147	25.29514225811827	25.90854496689672	24.25909546606187
10-11	24.35790002745565	25.510293483293044	25.91916204526396	24.212644443987347
12-13	24.00675009799152	25.60037086904277	26.138551013922513	24.254328019043196
14-15	23.670621949434505	25.910882125240086	26.64861182035719	23.76988410496822
16-17	23.402981546286618	25.800254642940935	26.985967510517956	23.810796300254495
18-19	22.87575201393984	25.84914985781863	27.90882005045967	23.36627807778186
20-21	22.440907008225665	25.479856765581527	28.953307809417854	23.125928416774954
22-23	22.36174620308654	25.38188839413075	29.511867785998575	22.744497616784134
24-25	22.26941652424608	25.220299531882684	30.114699622984165	22.39558432088707
26-27	22.328969345962335	25.237284174307895	30.22470108072706	22.209045399002715
28-29	22.347858067350646	25.33306817714671	30.17191022366397	22.14716353183867
30-31	22.332416460708306	25.283077510622782	30.13098506145855	22.253520967210367
32-33	22.386201579341	25.310407094029575	30.045234392351954	22.258156934277466
34-35	22.41429998807274	25.401497158727675	30.040056333868108	22.144146519331475
36-37	22.39493030997651	25.357557284340608	29.9841426487001	22.26336975698278
38-39	22.430863785701657	25.430634839578225	29.859435129099676	22.279066245620434
40-41	22.53125335137477	25.63861636319747	29.533250146575483	22.296880138852277
42-43	22.7104761632207	25.77121455620374	28.983501569980152	22.534807710595405
44-45	22.85158209820179	26.058202099209392	28.43377152071202	22.656444281876798
46-47	23.108079902598362	26.331616689571103	27.789350107191673	22.770953300638862
48-49	23.205005410833575	26.452136056268017	27.307805256734895	23.035053276163513
50-51	23.2299394157283	26.580339370893018	27.068019952893142	23.121701260485537
52-53	23.333397426323618	26.649436086022927	26.913023725436364	23.10414276221709
54-55	23.360443779199375	26.60536482626395	26.81291604150881	23.221275353027863
56-57	23.335197044614	26.644368710160748	26.857116495320497	23.163317749904753
58-59	23.394231090141005	26.696927654206636	26.816611376867083	23.092229878785275
60-61	23.346903816352295	26.69846819861465	26.762446250572342	23.192181734460718
62-63	23.375404933342388	26.74965006588727	26.76757480212017	23.107370198650173
64-65	23.424287382789036	26.888788747407617	26.64950513522909	23.03741873457426
66-67	23.453316205220194	26.946385823103814	26.504904721018935	23.095393250657057
68-69	23.4690146257752	27.071294132308456	26.39390432202286	23.065786919893494
70-71	23.552001626561246	27.212502281575606	26.264222442386348	22.9712736494768
72-73	23.532403605260466	27.26844704831456	26.142915930194103	23.056233416230874
74-75	23.564552733841342	27.402031630060247	26.068533958502254	22.964881677596157
76-77	23.403152968472448	27.612577977510362	25.925523572733244	23.058745481283943
78-79	23.425978058583755	27.679877888490367	25.820845888255086	23.073298164670796
80-81	23.41205126509567	27.797869593120467	25.716688191198184	23.073390950585683
82-83	23.433831402388687	27.930519534486248	25.629068558814772	23.006580504310293
84-85	23.428493354413213	27.96276252768426	25.50186736056984	23.10687675733268
86-87	23.42774417055082	28.082923528345745	25.426040031293628	23.063292269809807
88-89	23.490769814762764	28.189958591785302	25.247438995422417	23.071832598029516
90-91	23.499708364890306	28.250593432097997	25.10436849286693	23.145329710144765
92-93	23.49168503753918	28.38190994471597	24.98317928630092	23.143225731443927
94-95	23.543840933527513	28.49615478262256	24.83230493856797	23.127699345281954
96-97	23.51956068445049	28.568065665640503	24.664018019383153	23.24835563052585
98-99	23.54145650000895	28.679387263619944	24.559398096455194	23.219758139915914
100	23.933041560841232	28.65377941726528	23.914985018913164	23.498194002980327
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1907.0
1	1305.0
2	675.5
3	643.5
4	705.5
5	835.5
6	984.5
7	1136.5
8	1330.5
9	1608.5
10	2022.0
11	2658.5
12	3504.5
13	5251.5
14	9515.5
15	14079.5
16	20803.5
17	25185.0
18	27676.5
19	35406.5
20	62044.0
21	76285.5
22	76303.0
23	92431.5
24	111243.0
25	135345.5
26	165744.5
27	202122.0
28	244928.0
29	291002.5
30	334247.5
31	380440.0
32	432059.5
33	488570.5
34	551548.0
35	624494.5
36	704155.5
37	792884.0
38	888563.5
39	963159.5
40	1024782.5
41	1096819.0
42	1186193.5
43	1270011.0
44	1340283.5
45	1385141.5
46	1384678.0
47	1357269.5
48	1318889.0
49	1295755.5
50	1270524.5
51	1219257.0
52	1144388.0
53	1053723.5
54	976608.5
55	910801.0
56	829539.0
57	745681.5
58	676983.0
59	613987.5
60	549771.5
61	485969.0
62	425689.0
63	371638.5
64	324922.0
65	280024.0
66	238258.5
67	200402.0
68	164669.0
69	133134.5
70	106360.5
71	85353.5
72	68762.5
73	55560.0
74	44781.5
75	34824.5
76	26631.5
77	20806.0
78	16581.5
79	12383.0
80	8328.5
81	5379.5
82	3535.0
83	2273.0
84	1421.0
85	898.0
86	561.0
87	333.0
88	212.5
89	153.5
90	94.5
91	54.0
92	34.5
93	18.5
94	6.5
95	4.5
96	4.5
97	3.0
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15198981905207362
2	0.0
3	0.0
4	0.0
5	0.013969290812553044
6	8.525858242403266E-4
7	8.34699408347173E-5
8	0.0
9	0.0
10-11	0.0
12-13	4.620657439064708E-5
14-15	0.0
16-17	0.0
18-19	1.0433742604339662E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.9810693155256177E-6
30-31	4.471603973288426E-6
32-33	2.0718431742903045E-4
34-35	8.943207946576852E-6
36-37	1.4905346577628089E-6
38-39	0.0
40-41	2.235801986644213E-5
42-43	0.0
44-45	0.0
46-47	5.76836912554207E-4
48-49	8.078697845074424E-4
50-51	0.0
52-53	0.0
54-55	0.004417944725608965
56-57	0.002474287531886263
58-59	0.004774182508814277
60-61	0.01837680179555767
62-63	0.014212247961768381
64-65	0.01396780027789528
66-67	0.030642411494287827
68-69	0.010552985376960686
70-71	0.005522430907011207
72-73	0.008349975152787254
74-75	0.0011015051120867158
76-77	0.004783125716760854
78-79	0.0076121604971946645
80-81	0.010242954168146022
82-83	0.013854519643905307
84-85	0.005394244926443605
86-87	0.002393798660367071
88-89	0.008205393290984263
90-91	0.0015605897866776608
92-93	1.7886415893153705E-4
94-95	0.0012445964392319455
96-97	0.001387687766377175
98-99	0.002082276916894644
100	0.0011357874092152603
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.354501E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.91497556145046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.60814191566891	32.4472156255761
2	11.987779438293748	10.28910523260869
3	4.224064563553531	5.43826882544666
4	2.197098006236754	3.771536289750473
5	1.2646870452474224	2.713700681983806
6	0.8477605653395466	2.1828974366104843
7	0.5961937207056356	1.7909947267780804
8	0.43963736058008024	1.5093621268155781
9	0.34116655721387545	1.317703901869589
>10	2.107719052483562	17.32852469296943
>50	0.2244317605866284	6.719501433016611
>100	0.158184339596294	12.37405674891698
>500	0.0024636559301958903	0.6657386201308523
>1k	5.262469516718491E-4	0.46961668986627014
>5k	4.859053746188757E-5	0.16009150068055805
>10k+	9.718107480114788E-5	0.8216854669799368
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35559	0.10600384379077545	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34216	0.10200026770002453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1924277262102471E-5	0.0	0.0	2.9810693155256177E-6	0.0
2	1.1924277262102471E-5	0.0	0.0	5.9621386310512354E-6	0.0
3	1.7886415893153705E-5	2.9810693155256177E-6	0.0	5.9621386310512354E-6	0.0
4	1.7886415893153705E-5	2.9810693155256177E-6	0.0	5.9621386310512354E-6	0.0
5	2.0867485208679325E-5	5.9621386310512354E-6	0.0	5.9621386310512354E-6	0.0
6	2.3848554524204942E-5	5.9621386310512354E-6	0.0	5.9621386310512354E-6	0.0
7	2.682962383973056E-5	5.9621386310512354E-6	0.0	8.943207946576852E-6	0.0
8	4.471603973288426E-5	5.9621386310512354E-6	0.0	1.4905346577628088E-5	0.0
9	4.7697109048409884E-5	5.9621386310512354E-6	0.0	1.7886415893153705E-5	0.0
10-11	1.1775223796326191E-4	5.9621386310512354E-6	0.0	2.3848554524204942E-5	0.0
12-13	3.547472485475485E-4	5.9621386310512354E-6	0.0	2.3848554524204942E-5	0.0
14-15	0.0010239973098830496	1.341481191986528E-5	0.0	2.682962383973056E-5	1.4905346577628089E-6
16-17	0.0024310620268111413	1.7886415893153705E-5	0.0	2.832015849749337E-5	2.9810693155256177E-6
18-19	0.004382171893822658	1.7886415893153705E-5	2.9810693155256177E-6	2.9810693155256176E-5	2.9810693155256177E-6
20-21	0.007102397644239785	1.9376950550916515E-5	2.9810693155256177E-6	3.726336644407022E-5	2.9810693155256177E-6
22-23	0.010526155753120957	2.0867485208679325E-5	2.9810693155256177E-6	4.471603973288426E-5	2.9810693155256177E-6
24-25	0.014803990220900218	2.0867485208679325E-5	2.9810693155256177E-6	4.918764370617269E-5	2.9810693155256177E-6
26-27	0.019585625403003307	2.3848554524204942E-5	2.9810693155256177E-6	5.06781783639355E-5	2.9810693155256177E-6
28-29	0.02428677171358721	2.3848554524204942E-5	2.9810693155256177E-6	5.06781783639355E-5	2.9810693155256177E-6
30-31	0.0282948194083114	2.682962383973056E-5	2.9810693155256177E-6	5.514978233722393E-5	2.9810693155256177E-6
32-33	0.032137417756023925	2.682962383973056E-5	4.471603973288427E-6	5.962138631051235E-5	2.9810693155256177E-6
34-35	0.036152918124036926	2.682962383973056E-5	5.9621386310512354E-6	5.962138631051235E-5	2.9810693155256177E-6
36-37	0.04024294522493807	2.832015849749337E-5	5.9621386310512354E-6	5.962138631051235E-5	2.9810693155256177E-6
38-39	0.043654779056557146	3.130122781301898E-5	5.9621386310512354E-6	6.260245562603796E-5	2.9810693155256177E-6
40-41	0.046604547144269745	3.2791762470781796E-5	5.9621386310512354E-6	6.260245562603796E-5	2.9810693155256177E-6
42-43	0.04986136537148148	3.2791762470781796E-5	5.9621386310512354E-6	6.260245562603796E-5	2.9810693155256177E-6
44-45	0.05324487904460305	3.42822971285446E-5	8.943207946576852E-6	6.558352494156359E-5	2.9810693155256177E-6
46-47	0.0571068543428665	3.875390110183303E-5	8.943207946576852E-6	6.70740595993264E-5	2.9810693155256177E-6
48-49	0.06085554900713996	4.471603973288426E-5	8.943207946576852E-6	7.154566357261482E-5	2.9810693155256177E-6
50-51	0.06478757943431826	4.7697109048409884E-5	8.943207946576852E-6	7.154566357261482E-5	4.471603973288427E-6
52-53	0.06948276360627109	4.918764370617269E-5	8.943207946576852E-6	7.154566357261482E-5	5.9621386310512354E-6
54-55	0.07483080195832406	5.06781783639355E-5	8.943207946576852E-6	7.154566357261482E-5	5.9621386310512354E-6
56-57	0.0813936260564537	5.06781783639355E-5	8.943207946576852E-6	7.154566357261482E-5	7.452673288814044E-6
58-59	0.08880307384019262	5.06781783639355E-5	8.943207946576852E-6	8.048887151919167E-5	8.943207946576852E-6
60-61	0.09763300115277951	5.06781783639355E-5	8.943207946576852E-6	8.943207946576853E-5	8.943207946576852E-6
62-63	0.10843341528292882	5.664031699498674E-5	8.943207946576852E-6	8.943207946576853E-5	1.043374260433966E-5
64-65	0.12151732850877076	5.962138631051235E-5	8.943207946576852E-6	8.943207946576853E-5	1.1924277262102471E-5
66-67	0.1375629340995874	5.962138631051235E-5	8.943207946576852E-6	8.943207946576853E-5	1.1924277262102471E-5
68-69	0.15699652496749888	6.260245562603796E-5	8.943207946576852E-6	8.943207946576853E-5	1.1924277262102471E-5
70-71	0.180542500956178	6.409299028380078E-5	8.943207946576852E-6	8.943207946576853E-5	1.1924277262102471E-5
72-73	0.2097331316938048	6.558352494156359E-5	8.943207946576852E-6	9.092261412353133E-5	1.1924277262102471E-5
74-75	0.24526896846952795	6.558352494156359E-5	8.943207946576852E-6	9.241314878129415E-5	1.1924277262102471E-5
76-77	0.28785056257249586	6.558352494156359E-5	8.943207946576852E-6	9.837528741234538E-5	1.4905346577628088E-5
78-79	0.33868226600618095	6.558352494156359E-5	8.943207946576852E-6	1.0284689138563381E-4	1.4905346577628088E-5
80-81	0.3987940382190973	6.558352494156359E-5	1.043374260433966E-5	1.0582796070115943E-4	1.7886415893153705E-5
82-83	0.4711177608830643	6.70740595993264E-5	1.1924277262102471E-5	1.0880903001668504E-4	1.7886415893153705E-5
84-85	0.5559992380386829	6.85645942570892E-5	1.1924277262102471E-5	1.1477116864773627E-4	1.7886415893153705E-5
86-87	0.6551734520275891	6.85645942570892E-5	1.1924277262102471E-5	1.192427726210247E-4	1.9376950550916515E-5
88	0.7385360743669476	6.85645942570892E-5	1.4905346577628088E-5	1.192427726210247E-4	2.0867485208679325E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	32830	0.0	49.246395	1
TCTCGAG	39175	0.0	41.211483	2
TATCTCG	13315	0.0	33.9221	2
CTCGAGG	49485	0.0	32.539757	3
TCGAGGG	57785	0.0	27.86588	4
GATCCGG	48385	0.0	25.4378	1
ATATCTC	18595	0.0	24.805529	1
ATCCGGC	55850	0.0	22.09897	2
CGAGGGC	76205	0.0	21.191914	5
TCCGGCG	64980	0.0	19.001194	3
AGGGCGC	84815	0.0	18.938242	7
TATGGAT	25520	0.0	17.901363	2
GAGGGCG	90435	0.0	17.844593	6
GGGCGCG	92500	0.0	17.420717	8
ATGGATC	32940	0.0	16.960777	1
GCGCCGT	74710	0.0	16.448763	9
GGATCCG	33595	0.0	16.228704	3
CGGCGCG	75840	0.0	16.205927	5
CCGGCGC	77370	0.0	15.946199	4
ATATGGA	30455	0.0	15.176495	1
>>END_MODULE