FastQCFastQC Report
Wed 25 May 2016
SRR490990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47500857
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA345350.045.3599241
TCTCGAG409050.038.1566852
CTCGAGG520150.029.9344313
TCGAGGG615700.025.2278654
TATCTCG141400.024.8284342
GATCCGG579250.023.8744951
ATCCGGC667750.020.6642822
CGAGGGC834750.018.7146955
TCCGGCG770400.017.9902273
AGGGCGC878600.017.6776717
GGGCGCG958400.016.2742738
TATGGAT327450.016.1142121
GAGGGCG974150.016.0305826
GCGCCGT864650.015.8484389
GGATCCG402350.015.6406472
CGGCGCG890450.015.4275675
ATATCTC240450.015.1322381
CCGGCGC940700.014.6734074
CGTCGAC815650.013.8938412-13
ATGGATC441750.013.8094661
Produced by FastQC (version 0.11.5)