FastQCFastQC Report
Wed 25 May 2016
SRR490989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42851497
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA494500.11539853555174513No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435810.10170239793489595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA338250.046.6727871
TCTCGAG395650.039.8168032
CTCGAGG494500.031.7434223
TCGAGGG578700.027.0517084
TATCTCG148700.026.1377452
GATCCGG501650.024.367421
ATCCGGC583900.020.8385312
CGAGGGC783000.019.993395
AGGGCGC852950.018.225637
TCCGGCG685250.017.8250523
GAGGGCG921400.017.0246726
ATATCTC238750.016.7971131
GGGCGCG931450.016.7853878
GGATCCG349900.016.3866233
GCGCCGT767100.015.7624189
CGGCGCG784450.015.4511095
ATGGATC383300.015.3132061
CCGGCGC827600.014.7534034
TATGGAT314600.014.4457912
CGTCGAC731850.013.558310512-13
Produced by FastQC (version 0.11.5)