FastQCFastQC Report
Wed 25 May 2016
SRR490988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48887034
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT589550.12059434818647413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA414800.047.123641
TCTCGAG491050.039.602082
CTCGAGG624000.031.0439283
TCGAGGG736150.026.314494
TATCTCG188600.026.1638052
GATCCGG633500.024.433061
ATCCGGC744350.020.8158912
CGAGGGC988050.019.6152115
TCCGGCG867450.017.9702573
AGGGCGC1086400.017.7399857
ATATCTC286800.017.5682871
TATGGAT362750.017.0750222
GAGGGCG1170050.016.5640876
GGGCGCG1166700.016.5467078
GCGCCGT971200.015.9289479
GGATCCG448200.015.8957352
CGGCGCG1001750.015.4437855
ATGGATC460750.015.3405231
CCGGCGC1031050.015.0231414
TGGATCC514450.014.3616571
Produced by FastQC (version 0.11.5)