##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490988_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	48887034
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.776142565736347	33.0	31.0	34.0	30.0	34.0
2	32.05304357388505	34.0	31.0	34.0	30.0	34.0
3	32.125402228329094	34.0	31.0	34.0	30.0	34.0
4	35.47856067520889	37.0	35.0	37.0	33.0	37.0
5	35.28730002315133	37.0	35.0	37.0	33.0	37.0
6	35.21984334332903	37.0	35.0	37.0	33.0	37.0
7	35.19349110031916	37.0	35.0	37.0	33.0	37.0
8	35.139698473014334	37.0	35.0	37.0	33.0	37.0
9	36.81686156292484	39.0	37.0	39.0	33.0	39.0
10-11	36.75770883338924	39.0	37.0	39.0	33.0	39.0
12-13	36.71744046693445	39.0	37.0	39.0	33.0	39.0
14-15	38.01423685265913	40.5	38.0	41.0	33.0	41.0
16-17	37.94228093281339	40.0	38.0	41.0	33.0	41.0
18-19	37.87851355433017	40.0	38.0	41.0	33.0	41.0
20-21	37.793491613747726	40.0	38.0	41.0	32.5	41.0
22-23	37.676867224139635	40.0	38.0	41.0	32.0	41.0
24-25	37.555452167542015	40.0	38.0	41.0	32.0	41.0
26-27	37.30931411179496	40.0	37.5	41.0	31.5	41.0
28-29	37.050267858344604	40.0	37.0	41.0	31.0	41.0
30-31	36.78882580604092	40.0	36.5	41.0	30.0	41.0
32-33	36.57949887080488	40.0	36.0	41.0	30.0	41.0
34-35	36.43066128740803	40.0	36.0	41.0	30.0	41.0
36-37	36.44498558656677	40.0	36.0	41.0	30.0	41.0
38-39	36.37026047642817	40.0	36.0	41.0	30.0	41.0
40-41	36.19066396010034	40.0	36.0	41.0	29.0	41.0
42-43	35.872727418889845	40.0	35.5	41.0	27.5	41.0
44-45	35.51580146997668	40.0	35.0	41.0	25.5	41.0
46-47	35.13066399160154	40.0	35.0	41.0	23.5	41.0
48-49	34.77390207391187	39.0	35.0	41.0	21.0	41.0
50-51	34.49235591793112	39.0	35.0	41.0	18.5	41.0
52-53	34.21641704628675	39.0	34.0	41.0	16.5	41.0
54-55	33.88809773159893	39.0	34.0	41.0	12.0	41.0
56-57	33.51743947076028	38.0	33.0	40.5	7.5	41.0
58-59	33.168218417586964	38.0	33.0	40.0	4.5	41.0
60-61	32.75687885871743	37.5	32.5	40.0	2.0	41.0
62-63	32.36042762381534	37.0	32.0	40.0	2.0	41.0
64-65	31.891823995704055	36.0	31.0	40.0	2.0	41.0
66-67	31.339972598869466	36.0	31.0	39.0	2.0	41.0
68-69	30.88654282033146	35.0	30.0	39.0	2.0	41.0
70-71	30.429729424779588	35.0	30.0	38.5	2.0	40.0
72-73	29.960346019355562	35.0	29.0	37.5	2.0	40.0
74-75	29.377535626317602	35.0	29.0	37.0	2.0	39.0
76-77	28.697391377844685	34.0	28.5	36.0	2.0	39.0
78-79	28.724632425031146	34.0	29.0	36.0	2.0	39.0
80-81	28.33154852470698	34.0	27.5	36.0	2.0	37.5
82-83	27.84262672797863	34.0	26.5	35.0	2.0	37.0
84-85	27.34948923266648	34.0	26.0	35.0	2.0	36.5
86-87	26.84060692861833	34.0	25.0	35.0	2.0	36.0
88-89	26.391005424874006	34.0	23.5	35.0	2.0	36.0
90-91	25.885827334094355	33.0	21.0	35.0	2.0	35.5
92-93	25.40027459019093	33.0	18.5	35.0	2.0	35.0
94-95	24.85154070709219	33.0	8.0	35.0	2.0	35.0
96-97	24.238847605277098	32.5	2.0	35.0	2.0	35.0
98-99	23.614897796826867	32.0	2.0	35.0	2.0	35.0
100	22.65152569902277	31.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.22881610170579236
1101	2	-0.15174383794254354
1101	3	-0.10000854099394019
1101	4	-0.07551042437167865
1101	5	-0.2110578124356337
1101	6	-0.17850277168013662
1101	7	-0.1655033386926945
1101	8	-0.20885745805135514
1101	9	-0.2079070973020336
1101	10-11	-0.1576998985344602
1101	12-13	-0.19800981816378993
1101	14-15	-0.27379636726444545
1101	16-17	-0.21995557886813089
1101	18-19	-0.26237536504206815
1101	20-21	-0.3688754921447739
1101	22-23	-0.2998999741794748
1101	24-25	-0.36553893951411
1101	26-27	-0.3939026882903178
1101	28-29	-0.3916467752805062
1101	30-31	-0.39484649157848395
1101	32-33	-0.5366964357412698
1101	34-35	-0.5591012367558434
1101	36-37	-0.6323022592823122
1101	38-39	-0.5996828402817087
1101	40-41	-0.626625030140346
1101	42-43	-0.7311887766032825
1101	44-45	-0.7653386104193558
1101	46-47	-0.8745456387944799
1101	48-49	-0.9661080641235742
1101	50-51	-1.0126963016869368
1101	52-53	-1.035481945025623
1101	54-55	-1.0872426307858305
1101	56-57	-1.2153080640228993
1101	58-59	-1.240860161576471
1101	60-61	-1.2191598002228794
1101	62-63	-1.3840118456201012
1101	64-65	-1.4730426814773416
1101	66-67	-1.418608472468872
1101	68-69	-1.4689216563720109
1101	70-71	-1.5304006485186434
1101	72-73	-1.5875197268577779
1101	74-75	-1.6655651403035172
1101	76-77	-1.5008412414650643
1101	78-79	-1.4518926258611238
1101	80-81	-1.474446512216133
1101	82-83	-1.4913184765353336
1101	84-85	-1.603565604270912
1101	86-87	-1.7230614712185783
1101	88-89	-1.7521543240709043
1101	90-91	-1.7725152096603622
1101	92-93	-1.9444629838717198
1101	94-95	-2.136030527937443
1101	96-97	-2.216451392062279
1101	98-99	-2.330905481449836
1101	100	-2.288484892126238
1102	1	0.024773878790611548
1102	2	0.044861517663683514
1102	3	0.03950525912739522
1102	4	0.062013996910856406
1102	5	0.05897641195365111
1102	6	0.08226253163316954
1102	7	0.1019087937464036
1102	8	0.04745288892693367
1102	9	0.06925157677443394
1102	10-11	0.07647336597741372
1102	12-13	0.05336734398419907
1102	14-15	0.04583899434569361
1102	16-17	-0.0013369356510111174
1102	18-19	0.023844761129694803
1102	20-21	0.0060319963406669785
1102	22-23	0.016239110333550855
1102	24-25	-0.012017558526082439
1102	26-27	-0.03700822257751213
1102	28-29	-0.07957111568639874
1102	30-31	-0.069088327146126
1102	32-33	-0.14331791478652178
1102	34-35	-0.213430196882328
1102	36-37	-0.13853678313118678
1102	38-39	-0.18943358781874053
1102	40-41	-0.17221712381935106
1102	42-43	-0.2576591853825434
1102	44-45	-0.2224454505543889
1102	46-47	-0.31192335269062
1102	48-49	-0.38116802308523745
1102	50-51	-0.4828440398356122
1102	52-53	-0.5271595098068076
1102	54-55	-0.5703996015136354
1102	56-57	-0.5688560794562889
1102	58-59	-0.6011482329627569
1102	60-61	-0.6389501795268302
1102	62-63	-0.6919829312009433
1102	64-65	-0.7418631765548689
1102	66-67	-0.8438902162189414
1102	68-69	-0.8027597539884788
1102	70-71	-0.861783578916441
1102	72-73	-0.9532130949842639
1102	74-75	-1.0710048786976074
1102	76-77	-0.9724853996425296
1102	78-79	-0.898009121578081
1102	80-81	-0.9710907161794147
1102	82-83	-1.0119350361925505
1102	84-85	-1.0691835268192733
1102	86-87	-1.0893238354966996
1102	88-89	-1.1714129776048061
1102	90-91	-1.2338647481713068
1102	92-93	-1.4697254775928812
1102	94-95	-1.5014647888579908
1102	96-97	-1.5941210738267237
1102	98-99	-1.7136783816694
1102	100	-1.754645038660037
1103	1	0.07790021001430603
1103	2	0.08206333910758445
1103	3	0.07102899198125101
1103	4	0.08006211371086636
1103	5	0.10159058991462899
1103	6	0.10447733390116554
1103	7	0.11292117847847294
1103	8	0.10428212025330197
1103	9	0.10098960649724376
1103	10-11	0.1505534117855376
1103	12-13	0.13132438707043548
1103	14-15	0.1449926642569963
1103	16-17	0.18454937652376913
1103	18-19	0.16983634347432286
1103	20-21	0.19100831523154937
1103	22-23	0.21064413688231554
1103	24-25	0.1748499739681293
1103	26-27	0.18370670403580647
1103	28-29	0.13370796457728318
1103	30-31	0.13148525781275566
1103	32-33	0.10350021988139702
1103	34-35	0.040034463909158546
1103	36-37	0.1541139699047278
1103	38-39	0.1564208154343376
1103	40-41	0.178311019441189
1103	42-43	0.11914746567453705
1103	44-45	0.07358559850002422
1103	46-47	-1.535116854114449E-4
1103	48-49	4.963105680815261E-4
1103	50-51	0.0328477151942792
1103	52-53	-0.07790859232916603
1103	54-55	-0.1025062735080482
1103	56-57	-0.09489731077243135
1103	58-59	-0.005180718824419728
1103	60-61	0.009192261308896832
1103	62-63	-0.132845551556386
1103	64-65	-0.18063515225146887
1103	66-67	-0.17932149720848045
1103	68-69	-0.15580908519778802
1103	70-71	-0.1633867514411378
1103	72-73	-0.3199128617403524
1103	74-75	-0.25145815922057224
1103	76-77	-0.21054749106330561
1103	78-79	-0.19736164932421119
1103	80-81	-0.24066394227615362
1103	82-83	-0.27240786137445383
1103	84-85	-0.39488186836035766
1103	86-87	-0.4290866157988802
1103	88-89	-0.41952439436433053
1103	90-91	-0.4074523973662707
1103	92-93	-0.47798841540172887
1103	94-95	-0.6391301248497712
1103	96-97	-0.6954554454252495
1103	98-99	-0.7253138497324692
1103	100	-0.7371091698243895
1104	1	0.09812826983484513
1104	2	0.08985768469757716
1104	3	0.05892450209420019
1104	4	0.05423438994824181
1104	5	0.06625066383735856
1104	6	0.0690971521646091
1104	7	0.06338613995038855
1104	8	0.09578305093217665
1104	9	0.09352165867016993
1104	10-11	0.1338519047305624
1104	12-13	0.06931462383520426
1104	14-15	0.15918035116128948
1104	16-17	0.11936561058881523
1104	18-19	0.15505135475802234
1104	20-21	0.1727436412850949
1104	22-23	0.09908878877442362
1104	24-25	0.12861509567206042
1104	26-27	0.18538653611822298
1104	28-29	0.17205535019142815
1104	30-31	0.217977233960724
1104	32-33	0.1535576684131712
1104	34-35	0.18741290030991564
1104	36-37	0.22822839682605434
1104	38-39	0.26984575400427246
1104	40-41	0.25547768282989836
1104	42-43	0.19915355867664886
1104	44-45	0.21088816718866354
1104	46-47	0.18532961584935492
1104	48-49	0.1927812100623072
1104	50-51	0.22896174296366212
1104	52-53	0.24065629981571135
1104	54-55	0.2198634939654056
1104	56-57	0.18592824723646828
1104	58-59	0.013833877212142909
1104	60-61	0.09292739834195629
1104	62-63	0.08669084883466383
1104	64-65	0.20747931314989287
1104	66-67	0.2393696151717215
1104	68-69	0.25808569796819114
1104	70-71	0.18973046181423214
1104	72-73	0.142657468718685
1104	74-75	0.08215288439879131
1104	76-77	0.13836710055084467
1104	78-79	0.1998734387407488
1104	80-81	0.1933776313101987
1104	82-83	0.20834240739493737
1104	84-85	0.16277830707680607
1104	86-87	0.16195198930896382
1104	88-89	0.1859438827705624
1104	90-91	0.1815223105303332
1104	92-93	0.12497069376103198
1104	94-95	0.03382245608362666
1104	96-97	0.038567392240697274
1104	98-99	-0.03118620293092178
1104	100	-0.050995365949741966
1105	1	0.001475962651035445
1105	2	0.014916215135279742
1105	3	-0.011062440312066713
1105	4	-0.027335525386966708
1105	5	-0.06892758015218448
1105	6	0.01903655855480224
1105	7	0.03316180752499065
1105	8	0.029833718440272605
1105	9	-0.01755728502310916
1105	10-11	0.0478717240700135
1105	12-13	0.02774682180005783
1105	14-15	0.03254191988629884
1105	16-17	0.05686927511322892
1105	18-19	0.07520850961299885
1105	20-21	0.06411742218309513
1105	22-23	0.0798873258306827
1105	24-25	0.05915984286485809
1105	26-27	0.03003829700693217
1105	28-29	0.056478109934573695
1105	30-31	0.1107797160291426
1105	32-33	0.11193331632850345
1105	34-35	0.040199551840480297
1105	36-37	0.11430799805055614
1105	38-39	0.12168119004245881
1105	40-41	0.10420706782818456
1105	42-43	0.14991361447445684
1105	44-45	0.1632569749970756
1105	46-47	0.18677319303551343
1105	48-49	0.1730777117691673
1105	50-51	0.1476082663074294
1105	52-53	0.1978161916308494
1105	54-55	0.19425738059672426
1105	56-57	0.18024261026006627
1105	58-59	0.1789380940439429
1105	60-61	0.26629533553976614
1105	62-63	0.22020214456592413
1105	64-65	0.23981178041211493
1105	66-67	0.1734043907272742
1105	68-69	0.3091941727533509
1105	70-71	0.3282983193634337
1105	72-73	0.20925066336087284
1105	74-75	0.15996916833903896
1105	76-77	0.17603157352369436
1105	78-79	0.25471320125180696
1105	80-81	0.27444755991907854
1105	82-83	0.22837550084499725
1105	84-85	0.2585092276282843
1105	86-87	0.23653107681222352
1105	88-89	0.26526451032178855
1105	90-91	0.2687766144270185
1105	92-93	0.2599903526669429
1105	94-95	0.19263567276000515
1105	96-97	0.19212947434647987
1105	98-99	0.15072193127752698
1105	100	0.10685841024264775
1106	1	-0.09017930302125876
1106	2	-0.09033343335783073
1106	3	-0.09815052221983223
1106	4	-0.09102631742466372
1106	5	-0.14059231928769123
1106	6	-0.10022856756017973
1106	7	-0.07520729270341775
1106	8	-0.07909765945814229
1106	9	-0.0962898074307077
1106	10-11	-0.08943035821340573
1106	12-13	-0.10820903488770028
1106	14-15	-0.07730374746454771
1106	16-17	-0.054037049571320495
1106	18-19	-0.020061348522411038
1106	20-21	-0.012529043305427479
1106	22-23	-0.010439904349340168
1106	24-25	-0.038400456568922436
1106	26-27	-0.02026917420700869
1106	28-29	-0.011304757953197964
1106	30-31	0.02390421355740102
1106	32-33	0.043883519321447295
1106	34-35	0.05741539191173928
1106	36-37	0.007116397396799812
1106	38-39	0.06507878050972948
1106	40-41	0.10714809014973525
1106	42-43	0.12162809084892245
1106	44-45	0.11687721556547359
1106	46-47	0.09827137608731107
1106	48-49	0.18473926642684546
1106	50-51	0.2077210071073452
1106	52-53	0.1983646935726
1106	54-55	0.2566549566709426
1106	56-57	0.2518907008060651
1106	58-59	0.29738128040384737
1106	60-61	0.344489596632485
1106	62-63	0.1535050437199601
1106	64-65	0.29274799469555646
1106	66-67	0.3068869982205662
1106	68-69	0.3908054760546342
1106	70-71	0.3806061700269474
1106	72-73	0.39725363064534847
1106	74-75	0.41312146953161033
1106	76-77	0.36025797651841884
1106	78-79	0.4468372328324399
1106	80-81	0.4608611498511941
1106	82-83	0.4721875354850198
1106	84-85	0.4645994808108611
1106	86-87	0.45230001338303794
1106	88-89	0.44334518427916336
1106	90-91	0.5146364486138673
1106	92-93	0.6046959862574006
1106	94-95	0.5439898127778129
1106	96-97	0.5246094253933329
1106	98-99	0.5665527652004059
1106	100	0.5889388698812859
1107	1	-0.28762277132131686
1107	2	-0.2689541417263648
1107	3	-0.25363608413400485
1107	4	-0.1977846409648336
1107	5	-0.2227217779817181
1107	6	-0.18578832417028934
1107	7	-0.2029240666580705
1107	8	-0.2522965210969801
1107	9	-0.23470902644105252
1107	10-11	-0.25476634432039447
1107	12-13	-0.2689968394175466
1107	14-15	-0.24310214030727906
1107	16-17	-0.23964826484101565
1107	18-19	-0.29724083653933775
1107	20-21	-0.2979198159176448
1107	22-23	-0.3516631989182102
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2207	88-89	-0.25306417330939013
2207	90-91	-0.27069772649176116
2207	92-93	-0.23000666578512252
2207	94-95	-0.2891176547444161
2207	96-97	-0.2837379600313348
2207	98-99	-0.1791761175334834
2207	100	-0.08670232816671941
2208	1	-0.2519556421503495
2208	2	-0.24208742372419678
2208	3	-0.24420370147328185
2208	4	-0.2338021222335911
2208	5	-0.29293409007636484
2208	6	-0.3761441999241626
2208	7	-0.343220490486857
2208	8	-0.31932359137597643
2208	9	-0.3507642198857539
2208	10-11	-0.43797986116674537
2208	12-13	-0.38034959649513667
2208	14-15	-0.4708498993003545
2208	16-17	-0.6356919550505182
2208	18-19	-0.5759069659986196
2208	20-21	-0.5076926005084559
2208	22-23	-0.5141127960206759
2208	24-25	-0.43624564970630786
2208	26-27	-0.47360405371799885
2208	28-29	-0.40858619818313
2208	30-31	-0.3937196462893624
2208	32-33	-0.45075409792450927
2208	34-35	-0.30020665711696637
2208	36-37	-0.7146896668382823
2208	38-39	-0.6059508354548981
2208	40-41	-0.480244382518336
2208	42-43	-0.26230614131180374
2208	44-45	-0.18174863685721476
2208	46-47	-0.07871324783420164
2208	48-49	-0.02718470268219164
2208	50-51	-0.02374086924901775
2208	52-53	0.020860553354573597
2208	54-55	0.1119315044703626
2208	56-57	0.19620047019365217
2208	58-59	0.18985361911514076
2208	60-61	0.19892989845828168
2208	62-63	0.23488862144695588
2208	64-65	0.3736133475290693
2208	66-67	0.16323376089544084
2208	68-69	0.2270893160284615
2208	70-71	0.33986237870929514
2208	72-73	0.6187294162408143
2208	74-75	0.7957703033866395
2208	76-77	0.7196748552699859
2208	78-79	0.7476638551164392
2208	80-81	0.7139439084852022
2208	82-83	0.8611161714373736
2208	84-85	0.9842158235158465
2208	86-87	1.0879428816686953
2208	88-89	1.1439717406849113
2208	90-91	1.3058871959352132
2208	92-93	1.4036659476267594
2208	94-95	1.5995279574267158
2208	96-97	1.837126232216832
2208	98-99	1.8830273718200061
2208	100	1.8261597386910111
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	681981.0
3	206777.0
4	131055.0
5	110057.0
6	107883.0
7	118669.0
8	128817.0
9	140646.0
10	156279.0
11	166797.0
12	186521.0
13	229338.0
14	320330.0
15	460078.0
16	602863.0
17	591199.0
18	505253.0
19	401880.0
20	377253.0
21	386464.0
22	408965.0
23	443360.0
24	487481.0
25	543874.0
26	612020.0
27	694396.0
28	799678.0
29	924572.0
30	1080256.0
31	1274000.0
32	1537614.0
33	1915750.0
34	2503455.0
35	3472106.0
36	5239950.0
37	8458511.0
38	9830825.0
39	2644052.0
40	6029.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.70365326123732	28.10488218620838	27.918367818803304	22.273096733751004
2	23.988308392295117	27.337495145951994	27.26361870362676	21.410577758126124
3	23.686043215466906	26.021435458735336	26.914621165194845	23.377900160602913
4	24.526865344295587	25.731507867709873	26.708832448292934	23.032794339701606
5	24.83492657787339	25.986465041016803	26.19453657180348	22.98407180930633
6	24.928728678028566	25.782955596622443	25.730078457572482	23.55823726777651
7	24.74078432162749	26.249406876216103	25.891463385071354	23.118345417085052
8	24.32195973176439	25.876520415904547	26.176036708251367	23.6254831440797
9	24.21435152901409	25.6247332646375	26.316809697064315	23.844105509284095
10-11	24.02013394935694	25.8462842851938	26.301844079881782	23.83173768556748
12-13	23.70014926739231	25.924608056626262	26.52611137153898	23.849131304442448
14-15	23.370809280045325	26.17094967510808	27.023687979715476	23.434553065131116
16-17	23.15139327127107	26.096832751195336	27.27502143001762	23.47675254751597
18-19	22.681325374480473	26.116630304089767	28.11213408072041	23.08991024070935
20-21	22.322153968268967	25.784484082221066	29.03235037740273	22.861011572107238
22-23	22.274841832294427	25.726123004312353	29.51152242126205	22.487512742131173
24-25	22.19233324729825	25.557288871319134	30.035777993813245	22.21459988756937
26-27	22.247127939895066	25.589510093821605	30.106516586790683	22.05684537949265
28-29	22.27602449657015	25.679602207751845	30.06080940802874	21.983563887649265
30-31	22.259212830456164	25.61794883008894	30.018791596279666	22.104046743175232
32-33	22.313533135865562	25.65627285077675	29.928886176080212	22.101307837277478
34-35	22.35350201780599	25.72598391533883	29.92751853714627	21.99299552970891
36-37	22.32781629527086	25.698277089302184	29.870235002692592	22.103671612734363
38-39	22.355434766333364	25.751925931386744	29.76140014821119	22.1312391540687
40-41	22.459570043528878	25.917711284468055	29.494322279357576	22.128396392645495
42-43	22.608773250108978	26.043932935853437	28.999215118009474	22.34807869602811
44-45	22.710296393154543	26.290777796063846	28.51605249970296	22.48287331107865
46-47	22.953265321650544	26.515981976293435	27.982157414629377	22.54859528742664
48-49	23.042049321888747	26.597721127577973	27.577141004749112	22.783088545784164
50-51	23.04978050357306	26.700952711790954	27.369941446584097	22.87932533805189
52-53	23.144528314534387	26.771364824602202	27.228893178993875	22.85521368186954
54-55	23.155210465715616	26.751978902237454	27.15103741364296	22.94177321840397
56-57	23.145008184203313	26.783990525360657	27.157242850658548	22.913758439777478
58-59	23.220409731393453	26.8364436190406	27.127245174410714	22.815901475155233
60-61	23.172663994837663	26.823420183222414	27.078586925119648	22.925328896820282
62-63	23.18141788274457	26.874964215502178	27.07686422669236	22.866753675060895
64-65	23.230866989823973	27.00532608844211	26.98644639657014	22.777360525163772
66-67	23.261648128780685	27.040784153927405	26.844208796719105	22.853358920572813
68-69	23.281134757571014	27.147144423063157	26.74297174076823	22.8287490785976
70-71	23.343006674188835	27.280675889838225	26.649898392223907	22.726419043749036
72-73	23.353259487460775	27.300702294270955	26.531815297869173	22.8142229203991
74-75	23.35601170514075	27.429428426045334	26.47661747376105	22.73794239505287
76-77	23.215630978161098	27.630486007709738	26.34327107586842	22.81061193826075
78-79	23.265910954571144	27.673781655552126	26.22953106981954	22.830776320057193
80-81	23.243186329653387	27.803286594834393	26.11986252589269	22.833664549619527
82-83	23.258365437916638	27.941359353346208	26.030948012965627	22.76932719577153
84-85	23.25891205482153	27.954965282096566	25.933565010049968	22.85255765303194
86-87	23.24376632951785	28.097931234424117	25.818903145354405	22.83939929070363
88-89	23.343115127039475	28.163601407461407	25.68085114543538	22.812432320063742
90-91	23.319663948387266	28.232836575563837	25.53911988018516	22.90837959586374
92-93	23.332309520171556	28.334127446572676	25.466210207199012	22.867352826056756
94-95	23.400730921321745	28.441407926973245	25.29866491559859	22.85919623610642
96-97	23.33548664503536	28.54866859469205	25.134340262251275	22.981504498021312
98-99	23.39356632119864	28.613364824930475	25.018106880800854	22.97496197307003
100	23.798235754118725	28.55370205492931	24.501779653534815	23.146282537417157
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7246.0
1	4773.0
2	2178.5
3	2079.0
4	2190.5
5	2394.5
6	2689.5
7	3024.0
8	3492.5
9	4123.0
10	4900.5
11	6096.5
12	7846.0
13	11331.5
14	20399.0
15	29161.0
16	41272.0
17	46638.0
18	46410.0
19	56806.5
20	80235.5
21	98471.5
22	111451.0
23	136692.0
24	166278.5
25	202666.5
26	247903.0
27	302376.0
28	365668.5
29	432757.5
30	494991.5
31	563973.0
32	645264.0
33	735016.0
34	833209.0
35	945017.5
36	1068511.5
37	1208481.5
38	1355822.0
39	1468843.0
40	1560665.0
41	1665721.5
42	1800278.5
43	1926842.5
44	2026274.5
45	2081975.0
46	2070195.0
47	2024940.5
48	1961026.5
49	1911892.0
50	1852773.0
51	1756840.5
52	1633262.5
53	1485792.0
54	1359050.0
55	1252586.0
56	1129051.5
57	1005689.0
58	908156.5
59	820910.5
60	730996.5
61	644630.0
62	565028.5
63	492957.5
64	431596.0
65	371395.0
66	315029.0
67	265417.5
68	219142.5
69	177542.0
70	142275.5
71	114546.5
72	92866.0
73	76043.5
74	61484.5
75	47795.0
76	36701.0
77	28821.5
78	23191.5
79	17610.0
80	11907.0
81	7555.0
82	4900.0
83	3233.0
84	2072.0
85	1281.0
86	780.5
87	485.0
88	289.0
89	182.0
90	119.5
91	78.0
92	47.5
93	19.0
94	11.0
95	10.0
96	8.0
97	4.0
98	2.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20655783699211533
2	4.0910643096081465E-6
3	0.0
4	0.0
5	0.0
6	0.004119701759775404
7	0.007975529871581082
8	0.005212015930440779
9	0.004031743877118829
10-11	0.0025466875327310717
12-13	0.0012344786554242583
14-15	0.0014308497422854493
16-17	0.0
18-19	1.1250426851422403E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.2194023879624198E-4
30-31	0.0027655594732951074
32-33	0.002557937959582494
34-35	8.11053499379815E-4
36-37	1.7080193492614013E-4
38-39	7.773022188255479E-5
40-41	6.024092195897996E-4
42-43	0.0014400546369820676
44-45	8.090079672250111E-4
46-47	0.005397136590450547
48-49	0.004141179847400847
50-51	0.0023697490013405193
52-53	2.423955603442827E-4
54-55	0.003008977799716792
56-57	0.004105383034691775
58-59	0.013192659632408872
60-61	0.017196788825437844
62-63	0.019175841185210787
64-65	0.024067731333424727
66-67	0.023789538960371375
68-69	0.01444247977899416
70-71	0.02169798233208421
72-73	0.026749423988372868
74-75	0.009019774036608563
76-77	0.007977575403735887
78-79	0.01476874215768541
80-81	0.006269556054474486
82-83	0.009951513933121816
84-85	0.018431267480862105
86-87	0.014696125766189866
88-89	0.00234520261548287
90-91	0.0025968030705237712
92-93	0.009035115527769592
94-95	0.0021703096162471215
96-97	0.0022378121773556563
98-99	0.0032984205996215684
100	0.004577900962451516
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4.8887034E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.6210103869355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.044947855099	33.26354533180014
2	10.57227946723341	9.012024659730802
3	3.8358463524140083	4.904629416867784
4	2.0100165297156636	3.4267574156369345
5	1.2176175391967827	2.5948044892710467
6	0.7897774823319808	2.0196668566703475
7	0.5320037014105703	1.5872174698595631
8	0.39822432877494607	1.3578178602437916
9	0.3152893327178028	1.2094154932189896
>10	1.9141867783923805	15.65248078549763
>50	0.19504124161971256	5.786747227099614
>100	0.1615608918400252	14.138184040365351
>500	0.012058772855548005	3.3266030414657886
>1k	0.0010446204640744707	0.6970320978794785
>5k	2.3887796798775096E-5	0.06990825465969691
>10k+	8.12181374038111E-5	0.9531655597332211
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58955	0.12059434818647413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	2.0455321548040732E-6	0.0	2.0455321548040732E-6	0.0
2	2.0455321548040732E-6	1.4318725083628514E-5	0.0	4.0910643096081465E-6	0.0
3	2.0455321548040732E-6	1.6364257238432586E-5	0.0	4.0910643096081465E-6	0.0
4	2.0455321548040732E-6	2.454638585764888E-5	0.0	6.13659646441222E-6	0.0
5	4.0910643096081465E-6	2.454638585764888E-5	0.0	8.182128619216293E-6	0.0
6	4.0910643096081465E-6	3.272851447686517E-5	0.0	8.182128619216293E-6	0.0
7	8.182128619216293E-6	3.4774046631669246E-5	0.0	8.182128619216293E-6	2.0455321548040732E-6
8	1.0227660774020367E-5	3.681957878647332E-5	0.0	8.182128619216293E-6	2.0455321548040732E-6
9	1.0227660774020367E-5	4.295617525088554E-5	0.0	1.0227660774020367E-5	2.0455321548040732E-6
10-11	2.3523619780246844E-5	4.295617525088554E-5	0.0	1.227319292882444E-5	2.0455321548040732E-6
12-13	1.1659533282383218E-4	4.909277171529776E-5	0.0	1.227319292882444E-5	2.0455321548040732E-6
14-15	3.323989751556619E-4	5.420660210230794E-5	0.0	1.534149116103055E-5	2.0455321548040732E-6
16-17	8.151445636894232E-4	5.522936817970998E-5	0.0	1.6364257238432586E-5	2.0455321548040732E-6
18-19	0.001505511665935798	5.522936817970998E-5	0.0	1.840978939323666E-5	2.0455321548040732E-6
20-21	0.002344179849405468	5.829766641191609E-5	0.0	2.2500853702844807E-5	2.0455321548040732E-6
22-23	0.0036042276567647773	6.034319856672016E-5	2.0455321548040732E-6	2.6591918012452954E-5	2.0455321548040732E-6
24-25	0.005217129760827789	6.647979503113238E-5	2.0455321548040732E-6	3.3751280554267205E-5	2.0455321548040732E-6
26-27	0.007057085934074054	7.670745580515276E-5	2.0455321548040732E-6	3.681957878647332E-5	3.0682982322061097E-6
28-29	0.008891928276933306	8.386681834696701E-5	2.0455321548040732E-6	3.784234486387535E-5	4.0910643096081465E-6
30-31	0.010324823551373562	8.693511657917312E-5	2.0455321548040732E-6	3.988787701867943E-5	9.20489469661833E-6
32-33	0.011623736469674146	8.898064873397719E-5	2.0455321548040732E-6	4.0910643096081467E-5	1.227319292882444E-5
34-35	0.012986060884773661	9.409447912098738E-5	2.0455321548040732E-6	4.0910643096081467E-5	1.4318725083628514E-5
36-37	0.014449639141535974	9.716277735319348E-5	2.0455321548040732E-6	4.19334091734835E-5	1.4318725083628514E-5
38-39	0.015800713129784066	1.0023107558539959E-4	2.0455321548040732E-6	4.704723956049369E-5	1.4318725083628514E-5
40-41	0.017176333503889807	1.0534490597240978E-4	2.0455321548040732E-6	5.2161069947503875E-5	1.4318725083628514E-5
42-43	0.018722755812921682	1.1045873635941996E-4	4.0910643096081465E-6	5.522936817970998E-5	1.7387023315834623E-5
44-45	0.020439980056879702	1.1659533282383218E-4	5.113830387010183E-6	6.034319856672016E-5	1.9432555470638696E-5
46-47	0.022589834351578783	1.2580022752045053E-4	6.13659646441222E-6	6.238873072152424E-5	2.0455321548040733E-5
48-49	0.024976970376235138	1.3295959006226477E-4	6.13659646441222E-6	6.750256110853442E-5	2.0455321548040733E-5
50-51	0.027591160470074746	1.3909618652667698E-4	6.13659646441222E-6	6.954809326333849E-5	2.147808762544277E-5
52-53	0.030690141684602913	1.4421001691368717E-4	6.13659646441222E-6	7.159362541814257E-5	2.2500853702844807E-5
54-55	0.03432812062192196	1.5034661337809938E-4	6.13659646441222E-6	7.363915757294664E-5	2.2500853702844807E-5
56-57	0.039092165010460644	1.5443767768770754E-4	6.13659646441222E-6	7.568468972775072E-5	2.3523619780246844E-5
58-59	0.04472760609694587	1.5955150807471773E-4	6.13659646441222E-6	7.670745580515276E-5	2.5569151935050917E-5
60-61	0.05167014223035089	1.67733636693934E-4	6.13659646441222E-6	7.773022188255479E-5	2.6591918012452954E-5
62-63	0.05964158103762237	1.7080193492614013E-4	6.13659646441222E-6	7.773022188255479E-5	2.761468408985499E-5
64-65	0.06971684966610983	1.7796129746795437E-4	7.159362541814256E-6	7.977575403735887E-5	2.8637450167257028E-5
66-67	0.0821557306994734	1.810295957001605E-4	9.20489469661833E-6	7.977575403735887E-5	2.8637450167257028E-5
68-69	0.0975186999481294	1.861434260871707E-4	1.0227660774020367E-5	7.977575403735887E-5	2.8637450167257028E-5
70-71	0.11569836697395061	1.8818895824197475E-4	1.0227660774020367E-5	8.591235050177108E-5	2.8637450167257028E-5
72-73	0.13737589398448677	1.9125725647418086E-4	1.0227660774020367E-5	8.795788265657516E-5	3.272851447686517E-5
74-75	0.16427157352192812	1.9943938509339715E-4	1.0227660774020367E-5	8.795788265657516E-5	3.3751280554267205E-5
76-77	0.19717088993371945	2.086442797900155E-4	1.0227660774020367E-5	8.898064873397719E-5	3.681957878647332E-5
78-79	0.23671204925215958	2.1068981194481955E-4	1.4318725083628514E-5	9.000341481137923E-5	3.681957878647332E-5
80-81	0.2835025745272254	2.1273534409962363E-4	1.534149116103055E-5	9.307171304358535E-5	3.681957878647332E-5
82-83	0.33875035249632857	2.1682640840923177E-4	1.6364257238432586E-5	9.716277735319348E-5	3.681957878647332E-5
84-85	0.4035344013711284	2.1682640840923177E-4	1.6364257238432586E-5	1.0432213989500775E-4	3.784234486387535E-5
86-87	0.4810334781201903	2.198947066414379E-4	1.6364257238432586E-5	1.1352703459162607E-4	4.19334091734835E-5
88	0.5464250500449669	2.22963004873644E-4	1.6364257238432586E-5	1.1659533282383218E-4	4.295617525088554E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	41480	0.0	47.12364	1
TCTCGAG	49105	0.0	39.60208	2
CTCGAGG	62400	0.0	31.043928	3
TCGAGGG	73615	0.0	26.31449	4
TATCTCG	18860	0.0	26.163805	2
GATCCGG	63350	0.0	24.43306	1
ATCCGGC	74435	0.0	20.815891	2
CGAGGGC	98805	0.0	19.615211	5
TCCGGCG	86745	0.0	17.970257	3
AGGGCGC	108640	0.0	17.739985	7
ATATCTC	28680	0.0	17.568287	1
TATGGAT	36275	0.0	17.075022	2
GAGGGCG	117005	0.0	16.564087	6
GGGCGCG	116670	0.0	16.546707	8
GCGCCGT	97120	0.0	15.928947	9
GGATCCG	44820	0.0	15.895735	2
CGGCGCG	100175	0.0	15.443785	5
ATGGATC	46075	0.0	15.340523	1
CCGGCGC	103105	0.0	15.023141	4
TGGATCC	51445	0.0	14.361657	1
>>END_MODULE