FastQCFastQC Report
Wed 25 May 2016
SRR490987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36089791
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT479270.13279932820891094No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT430860.11938556252653278No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA406720.11269669031887716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA336000.051.430841
TCTCGAG403150.042.722682
CTCGAGG520600.033.0209923
TATCTCG137250.031.0218012
TCGAGGG618000.027.7710864
GATCCGG487000.025.7380661
ATATCTC203750.021.6309221
ATCCGGC581000.021.6292
CGAGGGC816200.021.1137285
AGGGCGC910650.018.7845347
TCCGGCG679550.018.4922853
TATGGAT275650.017.798922
GAGGGCG979000.017.5978816
GGGCGCG988900.017.3689488
GGATCCG337650.016.7575423
ATGGATC359350.016.1563131
GCGCCGT785250.015.8590019
CGGCGCG803150.015.5879195
CCGGCGC816150.015.3684184
ATATGGA328250.015.2914721
Produced by FastQC (version 0.11.5)