##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490987_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36089791
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.884262560567336	33.0	31.0	34.0	30.0	34.0
2	32.160027997945456	34.0	31.0	34.0	30.0	34.0
3	32.22951964448894	34.0	31.0	34.0	30.0	34.0
4	35.57911549002875	37.0	35.0	37.0	35.0	37.0
5	35.39882591727949	37.0	35.0	37.0	33.0	37.0
6	35.33485760557605	37.0	35.0	37.0	33.0	37.0
7	35.311441703832536	37.0	35.0	37.0	33.0	37.0
8	35.26047230364953	37.0	35.0	37.0	33.0	37.0
9	36.952508176065635	39.0	38.0	39.0	34.0	39.0
10-11	36.896287914219286	39.0	37.0	39.0	33.0	39.0
12-13	36.85864814235139	39.0	37.0	39.0	33.0	39.0
14-15	38.17309554383399	41.0	38.0	41.0	33.0	41.0
16-17	38.10206351707606	40.5	38.0	41.0	33.0	41.0
18-19	38.0341327551606	40.0	38.0	41.0	33.0	41.0
20-21	37.95071722637574	40.0	38.0	41.0	33.0	41.0
22-23	37.836480017299074	40.0	38.0	41.0	33.0	41.0
24-25	37.71459532697211	40.0	38.0	41.0	32.0	41.0
26-27	37.46105991580832	40.0	38.0	41.0	32.0	41.0
28-29	37.193256730137335	40.0	37.0	41.0	31.5	41.0
30-31	36.92569003793898	40.0	36.5	41.0	31.0	41.0
32-33	36.711436968421346	40.0	36.0	41.0	30.0	41.0
34-35	36.56241112618247	40.0	36.0	41.0	30.0	41.0
36-37	36.57521100634803	40.0	36.0	41.0	30.0	41.0
38-39	36.49617861461154	40.0	36.0	41.0	30.0	41.0
40-41	36.30343845715261	40.0	36.0	41.0	29.5	41.0
42-43	35.96452262635714	40.0	35.5	41.0	27.5	41.0
44-45	35.583967139072655	40.0	35.0	41.0	25.5	41.0
46-47	35.172400194836264	40.0	35.0	41.0	23.5	41.0
48-49	34.79627312056199	39.5	35.0	41.0	21.5	41.0
50-51	34.501488093959864	39.0	35.0	41.0	19.0	41.0
52-53	34.21610222126252	39.0	34.0	41.0	16.0	41.0
54-55	33.88055926951752	39.0	34.0	41.0	11.0	41.0
56-57	33.50405570373073	38.0	33.5	40.5	7.5	41.0
58-59	33.15014058961993	38.0	33.0	40.0	4.0	41.0
60-61	32.73719009622417	37.5	32.5	40.0	2.0	41.0
62-63	32.336390850808755	37.0	32.0	40.0	2.0	41.0
64-65	31.867427952131948	36.0	31.5	40.0	2.0	41.0
66-67	31.316589849467405	35.5	31.0	39.0	2.0	41.0
68-69	30.8590119294401	35.0	30.0	39.0	2.0	41.0
70-71	30.40069220683489	35.0	30.0	38.5	2.0	40.0
72-73	29.928487352559067	35.0	29.0	37.5	2.0	40.0
74-75	29.348726486113485	35.0	29.0	37.0	2.0	39.0
76-77	28.66487715320934	34.0	28.5	36.0	2.0	39.0
78-79	28.68710515669099	34.0	29.0	36.0	2.0	39.0
80-81	28.29587070759152	34.0	27.5	35.5	2.0	37.5
82-83	27.808272871405656	34.0	26.5	35.0	2.0	37.0
84-85	27.316263579913777	34.0	25.5	35.0	2.0	36.5
86-87	26.80822772013282	34.0	25.0	35.0	2.0	36.0
88-89	26.35731809862795	34.0	23.5	35.0	2.0	36.0
90-91	25.852851752452654	33.0	20.5	35.0	2.0	35.5
92-93	25.36701172639099	33.0	18.0	35.0	2.0	35.0
94-95	24.819447887631156	33.0	6.0	35.0	2.0	35.0
96-97	24.20716702127757	32.5	2.0	35.0	2.0	35.0
98-99	23.583247184778653	32.0	2.0	35.0	2.0	35.0
100	22.615127336148884	31.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.22147733114616486
1101	2	-0.1419571322853912
1101	3	-0.08763569337265409
1101	4	-0.07149682829991377
1101	5	-0.20800338868249213
1101	6	-0.18073734263411012
1101	7	-0.17256712039857547
1101	8	-0.21274524635680336
1101	9	-0.20632625936322313
1101	10-11	-0.15567552364991144
1101	12-13	-0.19710510811020043
1101	14-15	-0.2688424065565087
1101	16-17	-0.21656434678290282
1101	18-19	-0.24909550417152104
1101	20-21	-0.3403438522856419
1101	22-23	-0.28481743826217354
1101	24-25	-0.36912519717596126
1101	26-27	-0.3778143043658133
1101	28-29	-0.37287344216045426
1101	30-31	-0.3767623874731285
1101	32-33	-0.48932314688058653
1101	34-35	-0.5142849970155368
1101	36-37	-0.5914821453467312
1101	38-39	-0.5308072473123033
1101	40-41	-0.6046617291331629
1101	42-43	-0.6935119456537038
1101	44-45	-0.7179379921906772
1101	46-47	-0.8103538904379448
1101	48-49	-0.9032519243584218
1101	50-51	-0.9737329292133694
1101	52-53	-0.9751994424232038
1101	54-55	-1.0351086759601458
1101	56-57	-1.1231244556093145
1101	58-59	-1.178271552521835
1101	60-61	-1.1931752325219875
1101	62-63	-1.325877378665865
1101	64-65	-1.3788940023153984
1101	66-67	-1.3555353125553289
1101	68-69	-1.4049253737852325
1101	70-71	-1.4592621890439759
1101	72-73	-1.5024245186373513
1101	74-75	-1.5406922790960387
1101	76-77	-1.3957142485563274
1101	78-79	-1.3833641986760767
1101	80-81	-1.4032171598373324
1101	82-83	-1.4108874553839783
1101	84-85	-1.5205559163430635
1101	86-87	-1.6245453905046503
1101	88-89	-1.6443017761961087
1101	90-91	-1.6793755517892421
1101	92-93	-1.8145830464957697
1101	94-95	-1.9491202814523056
1101	96-97	-2.0560865347515076
1101	98-99	-2.174018507651315
1101	100	-2.1372189850797945
1102	1	0.03676143968406009
1102	2	0.055080939811603
1102	3	0.04708375319955849
1102	4	0.06441146877767778
1102	5	0.07620278865195473
1102	6	0.08919466616982419
1102	7	0.11543645375985534
1102	8	0.06569929839587729
1102	9	0.08545711478801366
1102	10-11	0.09592385279846383
1102	12-13	0.054662678161655265
1102	14-15	0.06543169120333658
1102	16-17	0.015307405227723336
1102	18-19	0.05376237385421234
1102	20-21	0.038387543701304594
1102	22-23	0.049573107727518106
1102	24-25	0.022133917196811126
1102	26-27	-0.01526796340942127
1102	28-29	-0.04913018964336402
1102	30-31	-0.04068463137458167
1102	32-33	-0.09038849462825738
1102	34-35	-0.17190396591340118
1102	36-37	-0.10810290799954458
1102	38-39	-0.169952609872702
1102	40-41	-0.14924435328912722
1102	42-43	-0.2119827107637562
1102	44-45	-0.19367753705895296
1102	46-47	-0.29357858663330916
1102	48-49	-0.3443131626342293
1102	50-51	-0.4258839960507288
1102	52-53	-0.4641177553283313
1102	54-55	-0.49870739375460715
1102	56-57	-0.4991012515379438
1102	58-59	-0.5345942168769682
1102	60-61	-0.5600196025608284
1102	62-63	-0.5998373780607125
1102	64-65	-0.6541072638595296
1102	66-67	-0.7151933696631758
1102	68-69	-0.6998297582349124
1102	70-71	-0.7495081177189746
1102	72-73	-0.8063989357905541
1102	74-75	-0.9107831894965379
1102	76-77	-0.8612164292259941
1102	78-79	-0.7688386437311152
1102	80-81	-0.8448074149577565
1102	82-83	-0.8900712929633592
1102	84-85	-0.9482724619257539
1102	86-87	-0.9726136129884182
1102	88-89	-1.0602206428235519
1102	90-91	-1.10149410718752
1102	92-93	-1.3131944125142994
1102	94-95	-1.3591578842842615
1102	96-97	-1.4510696067229212
1102	98-99	-1.5178853249551594
1102	100	-1.5365934534903758
1103	1	0.0785572114270714
1103	2	0.08265718450846293
1103	3	0.072015140345151
1103	4	0.07133462309168692
1103	5	0.09000912823874074
1103	6	0.10888069651879562
1103	7	0.10933121030387127
1103	8	0.0999431555420287
1103	9	0.10124229370865834
1103	10-11	0.15531832540266777
1103	12-13	0.12257593933751565
1103	14-15	0.15634356901296087
1103	16-17	0.20271882249984685
1103	18-19	0.19051914801774927
1103	20-21	0.19556589450380102
1103	22-23	0.22657941782834
1103	24-25	0.21038463900563897
1103	26-27	0.20997282526776928
1103	28-29	0.17382774888834973
1103	30-31	0.1697064423119201
1103	32-33	0.12352953617468643
1103	34-35	0.08266474219311704
1103	36-37	0.18877972535933907
1103	38-39	0.18515391132523717
1103	40-41	0.2115613712797071
1103	42-43	0.1427861909471133
1103	44-45	0.12057047475752114
1103	46-47	0.05521135445327019
1103	48-49	0.049256261862588246
1103	50-51	0.07743610247381838
1103	52-53	-0.04193360442846483
1103	54-55	-0.07076067962758259
1103	56-57	-0.03875256752030509
1103	58-59	0.022504321518944437
1103	60-61	0.07830761140669296
1103	62-63	-0.03584214037561395
1103	64-65	-0.1005527478514523
1103	66-67	-0.1258661466266453
1103	68-69	-0.08318351861377948
1103	70-71	-0.08979613875316872
1103	72-73	-0.20940059909936082
1103	74-75	-0.17989289023820376
1103	76-77	-0.1255924085688811
1103	78-79	-0.11374670002458487
1103	80-81	-0.16620116371758087
1103	82-83	-0.19906941980186232
1103	84-85	-0.30938049450768546
1103	86-87	-0.35558625999117766
1103	88-89	-0.3659074784916143
1103	90-91	-0.3707494380686782
1103	92-93	-0.4485903531612365
1103	94-95	-0.5721609417845777
1103	96-97	-0.6150160835780447
1103	98-99	-0.6420097887610119
1103	100	-0.6575096591512413
1104	1	0.09632896206506558
1104	2	0.0953796809377323
1104	3	0.053480391863622856
1104	4	0.07098924381129024
1104	5	0.051864267422516264
1104	6	0.07043384107217321
1104	7	0.07573829608282523
1104	8	0.09574433562659834
1104	9	0.0904200765418608
1104	10-11	0.1473240893576886
1104	12-13	0.08002560638482947
1104	14-15	0.15974214592210956
1104	16-17	0.11194473045599551
1104	18-19	0.1509873139831086
1104	20-21	0.14799731796721005
1104	22-23	0.08414056299197625
1104	24-25	0.10751764556489718
1104	26-27	0.15512057003773805
1104	28-29	0.1418058648003182
1104	30-31	0.19366280230554622
1104	32-33	0.14734661397709203
1104	34-35	0.16990270775998795
1104	36-37	0.18905704861244033
1104	38-39	0.2543509112852149
1104	40-41	0.24248620188209458
1104	42-43	0.20724552268558227
1104	44-45	0.22186423488974327
1104	46-47	0.19506278125511756
1104	48-49	0.19174618288811018
1104	50-51	0.21439116628736343
1104	52-53	0.24680369310224393
1104	54-55	0.21529114834487473
1104	56-57	0.18011428785078465
1104	58-59	0.01822200766260096
1104	60-61	0.08626440949502978
1104	62-63	0.06980008247237635
1104	64-65	0.17465761181604478
1104	66-67	0.22532882405610977
1104	68-69	0.2590818847238765
1104	70-71	0.19541995747903584
1104	72-73	0.13488951145960115
1104	74-75	0.10061217974175563
1104	76-77	0.13771053429970337
1104	78-79	0.187852809624367
1104	80-81	0.20502542647846766
1104	82-83	0.2198452813611489
1104	84-85	0.17938479352375225
1104	86-87	0.1654208897360263
1104	88-89	0.1727220701232035
1104	90-91	0.19833466540862332
1104	92-93	0.14662987377501935
1104	94-95	0.08938084395663992
1104	96-97	0.10099972575292782
1104	98-99	0.028067031964301492
1104	100	0.010252110738427689
1105	1	0.016490994339001475
1105	2	0.021563356587506632
1105	3	-0.002623276338546532
1105	4	-0.017175097046845167
1105	5	-0.04058242797630385
1105	6	0.022281871158845945
1105	7	0.051524386041087666
1105	8	0.03623852878442335
1105	9	0.015909310032569124
1105	10-11	0.07120582630668792
1105	12-13	0.04485207917617373
1105	14-15	0.04632007232061852
1105	16-17	0.06640391350162389
1105	18-19	0.09437080471953152
1105	20-21	0.09144249134996585
1105	22-23	0.1002589480675411
1105	24-25	0.09359894642156519
1105	26-27	0.06732091254094286
1105	28-29	0.08891424208986365
1105	30-31	0.14232795959868128
1105	32-33	0.14057642955180683
1105	34-35	0.09593757192656938
1105	36-37	0.1511704249835546
1105	38-39	0.15318375036833487
1105	40-41	0.14958661343204227
1105	42-43	0.1976313624076127
1105	44-45	0.18093127323185598
1105	46-47	0.20414234425837918
1105	48-49	0.1945096066688663
1105	50-51	0.1505295599300922
1105	52-53	0.1998982075204907
1105	54-55	0.22641578971067133
1105	56-57	0.18707048474745136
1105	58-59	0.17167825584771634
1105	60-61	0.24739584007922844
1105	62-63	0.2017264653850077
1105	64-65	0.23421374481709023
1105	66-67	0.15600289090140151
1105	68-69	0.3047150874568558
1105	70-71	0.3045201664419288
1105	72-73	0.19241298805738438
1105	74-75	0.13287993213070948
1105	76-77	0.15906707823191013
1105	78-79	0.2265244317838544
1105	80-81	0.2458634031673128
1105	82-83	0.22088235698256398
1105	84-85	0.275955815568814
1105	86-87	0.24901794341712247
1105	88-89	0.26835019792584447
1105	90-91	0.2710246472271223
1105	92-93	0.2470590376475741
1105	94-95	0.17973020665052886
1105	96-97	0.14548889247300067
1105	98-99	0.09691539325728371
1105	100	0.08132409230574922
1106	1	-0.09138008739899917
1106	2	-0.09066659084951567
1106	3	-0.09575260649392447
1106	4	-0.0899496690461632
1106	5	-0.1316146919570187
1106	6	-0.09098793319623866
1106	7	-0.06941640417716144
1106	8	-0.06021890073489544
1106	9	-0.08370907018515794
1106	10-11	-0.07417749354720371
1106	12-13	-0.10277220669838272
1106	14-15	-0.07409864500490215
1106	16-17	-0.054021497893977255
1106	18-19	-0.01993227215483273
1106	20-21	-0.02635522474325569
1106	22-23	-0.028287962228240815
1106	24-25	-0.05516428657228545
1106	26-27	-0.026076720084745375
1106	28-29	-0.023127161112462602
1106	30-31	0.00819611064419945
1106	32-33	0.019003375540307843
1106	34-35	0.02367712532068822
1106	36-37	-0.003017418675000272
1106	38-39	0.07401618284499989
1106	40-41	0.08860101314652269
1106	42-43	0.11689826494792044
1106	44-45	0.09617440746541916
1106	46-47	0.05083518346982885
1106	48-49	0.14968354214416735
1106	50-51	0.16209173691783718
1106	52-53	0.16488813270790104
1106	54-55	0.19794531444500763
1106	56-57	0.2011987520257179
1106	58-59	0.2412792476432486
1106	60-61	0.3037585151416806
1106	62-63	0.11583859850031786
1106	64-65	0.2654073803652608
1106	66-67	0.26246625799548795
1106	68-69	0.3247188913523118
1106	70-71	0.3185988790682863
1106	72-73	0.3196338025366394
1106	74-75	0.3426290048291385
1106	76-77	0.32337521959853177
1106	78-79	0.40143599635266725
1106	80-81	0.4050533925914941
1106	82-83	0.42268657013485367
1106	84-85	0.3953439709943751
1106	86-87	0.36125766957965055
1106	88-89	0.3486530397781884
1106	90-91	0.43847786968875724
1106	92-93	0.5257790811415646
1106	94-95	0.4747244867388467
1106	96-97	0.448413825552624
1106	98-99	0.47789867343424675
1106	100	0.4745426675497484
1107	1	-0.2745733798131482
1107	2	-0.2477720006640105
1107	3	-0.23916853154468143
1107	4	-0.17816727523365472
1107	5	-0.20259784698980354
1107	6	-0.17877631251361947
1107	7	-0.16702941181038256
1107	8	-0.22843837354469798
1107	9	-0.21387631530200224
1107	10-11	-0.23072610045310427
1107	12-13	-0.24345461755989817
1107	14-15	-0.2059630199262159
1107	16-17	-0.20563567244960979
1107	18-19	-0.2688811524230559
1107	20-21	-0.2782549285472342
1107	22-23	-0.34059397007850833
1107	24-25	-0.21583296534422658
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2207	90-91	-0.2997823153670893
2207	92-93	-0.2523791040709824
2207	94-95	-0.3176190273601911
2207	96-97	-0.28544344090128604
2207	98-99	-0.1563471001562995
2207	100	-0.08799900770496905
2208	1	-0.23750072936508104
2208	2	-0.2099789308308857
2208	3	-0.21528408432730117
2208	4	-0.20427828509942003
2208	5	-0.26456293991969204
2208	6	-0.3429150647743242
2208	7	-0.33646096330707564
2208	8	-0.2994926482251046
2208	9	-0.33036269004264085
2208	10-11	-0.41287165623614186
2208	12-13	-0.36619568372464784
2208	14-15	-0.44786015196098816
2208	16-17	-0.5936988851877416
2208	18-19	-0.5506142986238629
2208	20-21	-0.4771762472946932
2208	22-23	-0.484157666326972
2208	24-25	-0.39741384922673006
2208	26-27	-0.42342222741270064
2208	28-29	-0.37402443327799517
2208	30-31	-0.35436188153101256
2208	32-33	-0.3999382804772722
2208	34-35	-0.26417794665221095
2208	36-37	-0.6848629061695064
2208	38-39	-0.5593684370010976
2208	40-41	-0.4339439122434996
2208	42-43	-0.21252720887334675
2208	44-45	-0.11709891274515627
2208	46-47	-0.007474965304496095
2208	48-49	0.020406912024164114
2208	50-51	0.0535699592394252
2208	52-53	0.09391120669988595
2208	54-55	0.1574872420640716
2208	56-57	0.25218356138222475
2208	58-59	0.24843779693378565
2208	60-61	0.24882554502278253
2208	62-63	0.28045955321920957
2208	64-65	0.41314106743001133
2208	66-67	0.20096262132872056
2208	68-69	0.2903628966310592
2208	70-71	0.3899044044186333
2208	72-73	0.6388963879196439
2208	74-75	0.8018233362617195
2208	76-77	0.7623258670768003
2208	78-79	0.791974509047229
2208	80-81	0.7465497140432973
2208	82-83	0.8940807343540413
2208	84-85	1.0367950369972263
2208	86-87	1.126925452767395
2208	88-89	1.205819520784896
2208	90-91	1.3664404376728712
2208	92-93	1.4639712871762072
2208	94-95	1.6400269443354603
2208	96-97	1.8599159121302868
2208	98-99	1.9175368680469838
2208	100	1.845510225100746
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	446493.0
3	136827.0
4	89653.0
5	77686.0
6	77772.0
7	85916.0
8	93962.0
9	102080.0
10	113348.0
11	122037.0
12	134638.0
13	167414.0
14	236466.0
15	348633.0
16	468423.0
17	464563.0
18	396743.0
19	307953.0
20	282581.0
21	287512.0
22	303687.0
23	327139.0
24	359399.0
25	399687.0
26	450945.0
27	510472.0
28	588486.0
29	677822.0
30	790799.0
31	934722.0
32	1127861.0
33	1402339.0
34	1838943.0
35	2562908.0
36	3865241.0
37	6225047.0
38	7262041.0
39	2015173.0
40	6380.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.837466725210362	28.045727722346292	27.738506614362745	22.378298938080604
2	24.16527079627068	27.200655764450598	27.203817323615205	21.430256115663525
3	23.889639593645747	25.87960678409027	26.712210663674945	23.518542958589038
4	24.731949819271605	25.57470615443575	26.581032292484043	23.1123117338086
5	24.997626059956957	25.85492667441604	25.96941611548817	23.178031150138832
6	25.186585180905723	25.625737417382723	25.51912839804299	23.668549003668566
7	24.91854501229113	26.166737256925003	25.657010377488472	23.25770735329539
8	24.596664401982274	25.7170957143485	25.928983827670578	23.75725605599865
9	24.40716509408291	25.49233877363406	26.050597046837453	24.04989908544557
10-11	24.223673952709984	25.69182234267005	26.062386171809205	24.02211753281076
12-13	23.870879868339664	25.766162807191034	26.316692717375588	24.04626460709371
14-15	23.51914585349866	26.076105320454566	26.824498772097073	23.5802500539497
16-17	23.256547814311254	25.97260538305694	27.143922778605173	23.62692402402663
18-19	22.74484059304043	26.003803153981327	28.08645632596982	23.164899927008424
20-21	22.30475510373557	25.648177070352112	29.101990366195253	22.94507745971707
22-23	22.25850656768835	25.562902262304593	29.64385828668279	22.534732883324263
24-25	22.16308207492806	25.395248201908398	30.24023054054262	22.201439182620923
26-27	22.222107077317236	25.430245633730603	30.311015101195792	22.036632187756368
28-29	22.270721885080132	25.513726383938355	30.24809632384852	21.967455407132988
30-31	22.248088494100003	25.452864576579167	30.22130202968099	22.077744899639846
32-33	22.291321662078182	25.497194538419187	30.124532501579708	22.08695129792292
34-35	22.34498053747053	25.563956296234995	30.117406939942725	21.973656226351746
36-37	22.313671282720886	25.53243015773447	30.056831813402002	22.097066746142637
38-39	22.350304107734495	25.58798431449786	29.94652402712742	22.11518755064023
40-41	22.46082311711551	25.764044829093137	29.64708250970513	22.128049544086224
42-43	22.626999446913036	25.894503272115998	29.126977286581496	22.351519994389466
44-45	22.74355252378951	26.14620109824496	28.602028299679883	22.508218078285648
46-47	23.009449715222793	26.400387831254058	27.99840999161491	22.591752461908243
48-49	23.10799667337882	26.4877833292586	27.54664680647332	22.857573190889262
50-51	23.131967667752235	26.60811601388049	27.298424782584952	22.961491535782322
52-53	23.239905463318546	26.67845904488802	27.139507551948	22.942127939845435
54-55	23.267335950883687	26.62592427813423	27.05945189940875	23.04728787157333
56-57	23.24622774078709	26.66480779948342	27.053853691450445	23.035110768279043
58-59	23.319957462105133	26.724752942022185	27.015032824648767	22.94025677122391
60-61	23.26985617576367	26.71186189846994	26.958821360012596	23.05946056575379
62-63	23.289935336105135	26.744171625549278	26.969266348286986	22.9966266900586
64-65	23.34969267563139	26.875583002246238	26.86726838016154	22.907455941960833
66-67	23.37527155151711	26.922127992194028	26.721862429270775	22.980738027018084
68-69	23.397973608858507	27.00489818417714	26.629091340049115	22.968036866915234
70-71	23.485431871038678	27.138688261785642	26.51375262076412	22.86212724641156
72-73	23.493204773429717	27.16629703320016	26.383834749367	22.956663444003123
74-75	23.510038068384535	27.285711019740834	26.33653976050518	22.86771115136946
76-77	23.3517442245115	27.48752957532422	26.2041712802429	22.956554919921388
78-79	23.391311295317195	27.529757158111483	26.10424831622956	22.974683230341757
80-81	23.38357585873153	27.645531692001303	25.991052431443805	22.979840017823367
82-83	23.390827835535756	27.786843898998608	25.907313607612593	22.915014657853046
84-85	23.391431540140914	27.81965039255068	25.77690082394516	23.012017243363243
86-87	23.37406012185571	27.940456039470906	25.683403036356555	23.002080802316822
88-89	23.47056776378315	28.009478429379815	25.54093066396707	22.979023142869963
90-91	23.43989944245148	28.085417111893822	25.394251607926698	23.080431837727993
92-93	23.456428680383095	28.165691784135973	25.332715632081037	23.045163903399903
94-95	23.529203211578313	28.28821631154515	25.148930139610883	23.033650337265655
96-97	23.471273188607242	28.37961674031912	24.978829062142314	23.17028100893133
98-99	23.51971405460034	28.43859861660346	24.873964242637726	23.167723086158475
100	23.95054443062609	28.35767688207424	24.333582381479044	23.35819630582063
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8926.0
1	5874.0
2	2521.0
3	2239.5
4	2383.5
5	2608.5
6	2861.5
7	3260.0
8	3630.5
9	4157.0
10	4984.5
11	6035.0
12	7638.0
13	11105.0
14	20448.5
15	28690.0
16	39968.5
17	42811.5
18	38575.5
19	46128.0
20	68019.5
21	82323.5
22	87862.5
23	105669.0
24	127619.0
25	154745.5
26	188381.0
27	227968.5
28	272742.5
29	322453.0
30	368583.0
31	415156.5
32	470908.0
33	534599.0
34	602828.0
35	680022.5
36	764197.0
37	859774.5
38	964649.0
39	1044640.0
40	1105911.5
41	1179720.0
42	1279296.5
43	1369953.0
44	1443638.0
45	1488154.0
46	1478168.0
47	1447101.5
48	1408893.5
49	1385346.5
50	1355198.0
51	1294463.5
52	1212680.0
53	1114821.5
54	1030928.0
55	961434.5
56	874786.5
57	785110.5
58	714960.0
59	649886.5
60	580781.5
61	512688.5
62	448593.5
63	391471.0
64	342669.0
65	295181.0
66	251261.0
67	211616.5
68	174613.0
69	141504.0
70	113687.0
71	91689.0
72	74620.5
73	60886.5
74	48793.5
75	37621.5
76	28752.0
77	22666.0
78	18029.5
79	13441.5
80	9035.5
81	5843.0
82	3767.5
83	2446.0
84	1550.5
85	928.0
86	597.0
87	407.0
88	265.5
89	157.5
90	97.5
91	59.0
92	35.0
93	23.0
94	13.0
95	7.5
96	4.0
97	2.5
98	2.5
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20965485779621168
2	8.312600092364071E-6
3	0.0
4	0.0
5	0.0
6	0.005126103390291177
7	0.009983432710929248
8	0.0065087658723210675
9	0.0050845403898293565
10-11	0.0031490900016572553
12-13	0.001557227083969536
14-15	0.001715166485724453
16-17	0.0
18-19	2.3552366928364867E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.175130357501932E-4
30-31	0.0026794280964386854
32-33	0.0024910091610117666
34-35	8.714375763495001E-4
36-37	1.5932483510364468E-4
38-39	8.728230096982275E-5
40-41	6.553099739480342E-4
42-43	0.0014671739163022585
44-45	8.354163092825891E-4
46-47	0.005350543592785007
48-49	0.0042172591135260385
50-51	0.002405112293390671
52-53	2.660032029556503E-4
54-55	0.003011932100133248
56-57	0.004165998079623126
58-59	0.013271066047459238
60-61	0.017563138561816554
62-63	0.01934480584827992
64-65	0.024318511570211088
66-67	0.024106540267855806
68-69	0.014675895463068765
70-71	0.022154464679498977
72-73	0.027445434638288704
74-75	0.00946528063850522
76-77	0.008059065789546967
78-79	0.015095681767733153
80-81	0.00646581743851052
82-83	0.010180164246448532
84-85	0.01886128960957408
86-87	0.014941898666024417
88-89	0.0024951654610579485
90-91	0.0027805647308957815
92-93	0.009161870735133934
94-95	0.0022347039914972075
96-97	0.0022541000583793905
98-99	0.0033887699709870866
100	0.004771432453016976
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.6089791E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.495996249803156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.38106024445428	35.20528426687484
2	11.470533372378986	10.437266865859925
3	4.048433771522976	5.525625830603574
4	2.0133998864873353	3.6640653473992764
5	1.1428457137249035	2.599745215286591
6	0.7400840171751333	2.020251580196347
7	0.510356874004987	1.6253436098054501
8	0.37560752352461513	1.3670910785338992
9	0.2788700971548818	1.141872560490667
>10	1.7194053559142413	14.760656052224508
>50	0.17000114545370407	5.403552943923232
>100	0.14333992649012997	13.003870782298744
>500	0.005308779273340247	1.4999441441631696
>1k	6.137538511616481E-4	0.48193144109803693
>5k	4.246826940717637E-5	0.13648057878944378
>10k+	9.707031989433277E-5	1.1270177024524457
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47927	0.13279932820891094	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43086	0.11938556252653278	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40672	0.11269669031887716	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	2.77086669745469E-6
4	0.0	0.0	0.0	0.0	2.77086669745469E-6
5	0.0	0.0	0.0	2.77086669745469E-6	2.77086669745469E-6
6	2.77086669745469E-6	0.0	0.0	2.77086669745469E-6	2.77086669745469E-6
7	2.77086669745469E-6	0.0	0.0	2.77086669745469E-6	2.77086669745469E-6
8	2.77086669745469E-6	0.0	0.0	8.31260009236407E-6	2.77086669745469E-6
9	8.31260009236407E-6	2.77086669745469E-6	0.0	8.31260009236407E-6	2.77086669745469E-6
10-11	3.879213376436566E-5	4.156300046182035E-6	0.0	1.5239766836000795E-5	2.77086669745469E-6
12-13	2.2444020249382993E-4	5.54173339490938E-6	0.0	1.939606688218283E-5	2.77086669745469E-6
14-15	6.497682405531248E-4	5.54173339490938E-6	0.0	2.3552366928364867E-5	2.77086669745469E-6
16-17	0.0015225912502513522	5.54173339490938E-6	0.0	2.4937800277092213E-5	2.77086669745469E-6
18-19	0.002806887964521601	5.54173339490938E-6	0.0	2.9094100323274248E-5	2.77086669745469E-6
20-21	0.004433386715927504	8.31260009236407E-6	0.0	3.602126706691097E-5	6.927166743636725E-6
22-23	0.006661163540681075	8.31260009236407E-6	0.0	4.1563000461820356E-5	1.108346678981876E-5
24-25	0.009551177506126317	8.31260009236407E-6	0.0	4.710473385672973E-5	1.108346678981876E-5
26-27	0.012869290376328309	1.108346678981876E-5	0.0	4.710473385672973E-5	1.108346678981876E-5
28-29	0.0161874032465303	1.385433348727345E-5	0.0	4.849016720545708E-5	1.108346678981876E-5
30-31	0.018940259310451533	1.385433348727345E-5	0.0	4.9875600554184425E-5	1.108346678981876E-5
32-33	0.021352298770585844	1.5239766836000795E-5	0.0	5.1261033902911765E-5	1.108346678981876E-5
34-35	0.023833609898156517	1.662520018472814E-5	0.0	5.403190060036646E-5	1.2468900138546105E-5
36-37	0.026464547827389745	1.662520018472814E-5	0.0	5.8188200646548495E-5	1.662520018472814E-5
38-39	0.02885719122064187	1.662520018472814E-5	2.77086669745469E-6	5.8188200646548495E-5	1.662520018472814E-5
40-41	0.030989373144333254	1.8010633533455486E-5	2.77086669745469E-6	6.234450069273053E-5	1.662520018472814E-5
42-43	0.03329889053666174	2.216693357963752E-5	5.54173339490938E-6	6.650080073891256E-5	2.216693357963752E-5
44-45	0.03580652489785824	2.3552366928364867E-5	5.54173339490938E-6	7.06571007850946E-5	2.216693357963752E-5
46-47	0.03861479829572856	2.6323233625819555E-5	5.54173339490938E-6	7.204253413382194E-5	2.216693357963752E-5
48-49	0.041507583127871256	2.77086669745469E-5	5.54173339490938E-6	7.758426752873133E-5	2.216693357963752E-5
50-51	0.044627579029205236	3.047953367200159E-5	5.54173339490938E-6	8.312600092364071E-5	2.216693357963752E-5
52-53	0.048208924235665425	3.602126706691097E-5	5.54173339490938E-6	8.589686762109539E-5	2.493780027709221E-5
54-55	0.052482986116489286	3.879213376436566E-5	5.54173339490938E-6	8.728230096982274E-5	3.047953367200159E-5
56-57	0.057707455274540104	3.879213376436566E-5	5.54173339490938E-6	9.282403436473212E-5	3.047953367200159E-5
58-59	0.06377011160857096	3.879213376436566E-5	5.54173339490938E-6	9.698033441091416E-5	3.047953367200159E-5
60-61	0.0709812921886968	4.017756711309301E-5	5.54173339490938E-6	9.975120110836885E-5	3.047953367200159E-5
62-63	0.07986053452069035	4.1563000461820356E-5	5.54173339490938E-6	1.0390750115455089E-4	3.047953367200159E-5
64-65	0.09085671900953929	4.1563000461820356E-5	5.54173339490938E-6	1.0529293450327822E-4	3.325040036945628E-5
66-67	0.10421368192461963	4.1563000461820356E-5	5.54173339490938E-6	1.0529293450327822E-4	3.325040036945628E-5
68-69	0.12075159980837795	4.1563000461820356E-5	5.54173339490938E-6	1.0529293450327822E-4	3.325040036945628E-5
70-71	0.1401490521239095	4.1563000461820356E-5	8.31260009236407E-6	1.0667836785200557E-4	3.4635833718183625E-5
72-73	0.16385658758733185	4.1563000461820356E-5	8.31260009236407E-6	1.0944923454946026E-4	3.602126706691097E-5
74-75	0.19292020837693408	4.1563000461820356E-5	1.108346678981876E-5	1.1499096794436964E-4	3.879213376436566E-5
76-77	0.22784698309835044	4.433386715927504E-5	1.2468900138546105E-5	1.2191813468800637E-4	4.571930050800239E-5
78-79	0.2692797528253904	4.433386715927504E-5	1.385433348727345E-5	1.288453014316431E-4	4.849016720545708E-5
80-81	0.31802761063371077	4.987560055418442E-5	1.385433348727345E-5	1.3300160147782512E-4	4.9875600554184425E-5
82-83	0.37555080327287016	5.54173339490938E-5	1.385433348727345E-5	1.357724681752798E-4	5.1261033902911765E-5
84-85	0.4435547991951519	6.234450069273053E-5	1.385433348727345E-5	1.357724681752798E-4	5.264646725163911E-5
86-87	0.5222598823030037	6.372993404145788E-5	1.385433348727345E-5	1.357724681752798E-4	5.403190060036646E-5
88	0.588726047208198	6.372993404145788E-5	1.385433348727345E-5	1.385433348727345E-4	5.8188200646548495E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	33600	0.0	51.43084	1
TCTCGAG	40315	0.0	42.72268	2
CTCGAGG	52060	0.0	33.020992	3
TATCTCG	13725	0.0	31.021801	2
TCGAGGG	61800	0.0	27.771086	4
GATCCGG	48700	0.0	25.738066	1
ATATCTC	20375	0.0	21.630922	1
ATCCGGC	58100	0.0	21.629	2
CGAGGGC	81620	0.0	21.113728	5
AGGGCGC	91065	0.0	18.784534	7
TCCGGCG	67955	0.0	18.492285	3
TATGGAT	27565	0.0	17.79892	2
GAGGGCG	97900	0.0	17.597881	6
GGGCGCG	98890	0.0	17.368948	8
GGATCCG	33765	0.0	16.757542	3
ATGGATC	35935	0.0	16.156313	1
GCGCCGT	78525	0.0	15.859001	9
CGGCGCG	80315	0.0	15.587919	5
CCGGCGC	81615	0.0	15.368418	4
ATATGGA	32825	0.0	15.291472	1
>>END_MODULE