FastQCFastQC Report
Wed 25 May 2016
SRR490986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42243898
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453480.10734804823172332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA328600.046.0967861
TCTCGAG393600.038.3784642
CTCGAGG484900.031.113413
TATCTCG143100.028.2130722
TCGAGGG591450.025.4209044
GATCCGG572700.024.4341741
ATCCGGC667700.020.884932
CGAGGGC812550.018.5442055
TCCGGCG776050.018.0052833
TATGGAT312500.017.100442
AGGGCGC872300.017.094947
ATATCTC245050.016.7603841
GGATCCG397850.016.6569942
ATGGATC400950.015.99959851
GCGCCGT879550.015.8370549
GGGCGCG960900.015.6163118
GAGGGCG972450.015.4745866
CGGCGCG911200.015.2676715
CCGGCGC935400.014.9379954
TGGATCC458050.014.879141
Produced by FastQC (version 0.11.5)