FastQCFastQC Report
Wed 25 May 2016
SRR490985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47990468
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660590.1376502517124859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA422750.049.503561
TCTCGAG506100.041.271822
CTCGAGG634250.032.8583073
TCGAGGG769000.027.008954
TATCTCG208750.026.0959342
GATCCGG702350.025.504481
ATCCGGC828700.021.609442
CGAGGGC1043900.019.9144385
ATATCTC285700.019.5367281
TATGGAT367450.018.5603032
TCCGGCG967550.018.5372353
AGGGCGC1155650.017.8695417
GGGCGCG1258050.016.5005468
GAGGGCG1268300.016.408576
GCGCCGT1089900.016.3123079
GGATCCG485950.016.2395882
ATGGATC468000.016.2342511
CGGCGCG1129450.015.8009635
CCGGCGC1136700.015.7208584
TGGATCC526150.015.3710821
Produced by FastQC (version 0.11.5)