FastQCFastQC Report
Wed 25 May 2016
SRR490984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22364554
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT297510.1330274683769683No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT259150.11587532664411729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG130900.047.073322
ATCTCGA212400.037.2802731
ATATCTC173000.036.355321
TCTCGAG238000.033.0195432
TATGGAT171500.030.42082
CTCGAGG296450.026.4774883
ATGGATC206950.025.2552173
TCGAGGG343800.022.803884
ATATGGA235250.022.5528741
TGGATCC236900.021.8047524
GGATCCG237600.021.3250435
GATCCGG308750.017.1077731
CGAGGGC471200.016.6781775
AGGGCGC484800.016.0729587
ATCCGGC343350.015.2496522
GGGCGCG515200.015.069828
GAGGGCG532500.014.7126476
GCGCCGT366300.014.1903499
TCCGGCG384200.013.7261033
CGTCGAC350600.012.18487412-13
Produced by FastQC (version 0.11.5)