FastQCFastQC Report
Wed 25 May 2016
SRR490983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19792800
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT518770.2621003597267693No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT416750.21055636393031812No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT232860.11764884200315265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG175800.059.2732162
ATATCTC201050.052.265821
TATGGAT190900.039.3443642
ATCTCGA268250.038.652493
ATGGATC222050.033.8673253
TCTCGAG304050.033.854064
ATATGGA240200.031.7518981
TGGATCC260200.028.7572574
CTCGAGG359250.028.6652095
GGATCCG284400.025.781285
TCGAGGG418750.024.52316
GATCCGG376050.019.5340426
CGAGGGC555650.018.5318477
ATCCGGC408150.017.801947
AGGGCGC611850.016.66839
GCGCCGT424050.016.2699249
TCCGGCG452550.015.993078
GAGGGCG645100.015.8966418
CGGCGCG495200.013.94282155
GGGCGCG654550.013.800298
Produced by FastQC (version 0.11.5)