FastQCFastQC Report
Wed 25 May 2016
SRR490982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32376268
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT9832643.03698993349079No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT7283182.2495427823861602No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA913100.28202756414049945No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC874200.2700125907037834No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG864340.2669671501360194No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA854620.2639649511178991No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA705350.21786019315135396No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG629990.19458388471456933No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA564160.17425109033567426No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC462620.1428886121155162No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG442620.13671124788070077No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA359220.11095163902152033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG1937200.088.76192
ATATCTC1968900.087.659011
TATGGAT1865300.084.599442
ATCTCGA2037250.084.061363
ATGGATC1887000.083.3750153
GGATCCG1880000.083.055565
TCTCGAG2073050.082.39874
ATATGGA1940600.081.762331
TGGATCC1931900.080.921594
CTCGAGG2153950.079.310435
GATCCGG1993200.078.346316
TCGAGGG2224300.076.815146
ATCCGGC2061000.075.591587
TCCGGCG2136650.073.0868768
CGAGGGC2396500.071.292127
CCGGCGC2216900.070.655199
AGGGCGC2463750.069.384299
GAGGGCG2506800.068.241598
CCGTCGA2163500.036.82296816-17
GTCGACT2172000.036.6514218-19
Produced by FastQC (version 0.11.5)