FastQCFastQC Report
Wed 25 May 2016
SRR490981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34098900
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT13033983.8224048283082444No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT7971512.337761628674239No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA1068260.3132828331705715No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG1036060.3038397133045348No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC1003100.29417371234849216No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA914370.26815234509031083No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA824830.241893433512518No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG761530.22332978483176877No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA606160.17776526515518096No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG547940.16069140060236548No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC527820.15479091700905306No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA434210.12733841854136058No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC383870.11257547897439507No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTGG382010.11203000683306501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG2200500.089.814732
ATATCTC2244900.088.3776551
TATGGAT2349200.087.192552
ATCTCGA2274650.086.500533
ATGGATC2394200.085.282843
GGATCCG2385900.085.074095
TCTCGAG2310750.084.995544
ATATGGA2430550.084.579591
TGGATCC2447400.083.036154
CTCGAGG2382450.082.443515
GATCCGG2492150.081.43766
TCGAGGG2448250.080.2162556
ATCCGGC2535800.079.759757
TCCGGCG2596500.077.972998
CGAGGGC2599450.075.604737
CCGGCGC2686400.075.556069
AGGGCGC2613050.075.288579
GAGGGCG2685550.073.2962958
CCGTCGA2609500.039.3614816-17
GTCGACT2621000.039.15152718-19
Produced by FastQC (version 0.11.5)