FastQCFastQC Report
Wed 25 May 2016
SRR490980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31915232
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT9567642.997828748354391No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT6860072.149465809930506No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA993030.3111461010216062No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA690870.2164703048375146No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA582090.1823862662192147No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG562760.1763295971027251No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC485240.15204025463452686No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA473930.1484964922078586No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG388030.12158144424580715No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA363620.11393305867242326No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340790.10677973451673484No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT334890.1049310874506568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG1451550.087.8188252
ATATCTC1483800.086.461541
TATGGAT1762700.084.245482
GGATCCG1759750.083.521935
ATGGATC1779950.083.130353
ATCTCGA1550000.081.679483
ATATGGA1829700.081.672331
TGGATCC1814250.081.1697544
TCTCGAG1593600.079.276444
GATCCGG1867250.078.787716
CTCGAGG1664250.075.890845
ATCCGGC1944150.075.5194557
TCGAGGG1726650.073.11276
TCCGGCG2027350.072.703718
CCGGCGC2143750.069.207699
CGAGGGC1883050.066.961447
AGGGCGC1958000.064.550059
GAGGGCG2003550.063.0520368
CCGTCGA1902300.036.37823516-17
GTCGACT1913300.036.11159518-19
Produced by FastQC (version 0.11.5)