##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490980_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31915232
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.281286753610313	33.0	31.0	34.0	28.0	34.0
2	31.54390019787417	34.0	31.0	34.0	28.0	34.0
3	31.652099223342635	34.0	31.0	34.0	28.0	34.0
4	35.01832930432716	37.0	35.0	37.0	32.0	37.0
5	34.843024954354085	37.0	35.0	37.0	32.0	37.0
6	34.75518470302832	37.0	35.0	37.0	32.0	37.0
7	34.751787798377904	37.0	35.0	37.0	32.0	37.0
8	34.67432823298919	37.0	35.0	37.0	32.0	37.0
9	36.195278511527036	39.0	37.0	39.0	32.0	39.0
10-11	36.124637007182024	39.0	37.0	39.0	31.5	39.0
12-13	35.99774660889196	39.0	37.0	39.0	31.0	39.0
14-15	37.13773558030222	40.0	37.5	41.0	31.0	41.0
16-17	37.01312149321052	40.0	37.0	41.0	31.0	41.0
18-19	36.92473836630735	40.0	37.0	41.0	30.0	41.0
20-21	36.603665610201425	40.0	37.0	41.0	30.0	41.0
22-23	36.430307634924915	40.0	36.5	41.0	29.0	41.0
24-25	36.397098711361394	40.0	36.0	41.0	29.0	41.0
26-27	36.350760242006075	40.0	36.0	41.0	29.0	41.0
28-29	36.38801669998827	40.0	36.0	41.0	30.0	41.0
30-31	36.18917828953899	39.5	36.0	41.0	29.0	41.0
32-33	35.57699096469047	39.0	35.0	41.0	27.5	41.0
34-35	35.306682605346566	39.0	35.0	41.0	26.5	41.0
36-37	35.08364220883621	39.0	35.0	41.0	25.0	41.0
38-39	34.8500253107983	39.0	35.0	41.0	24.0	41.0
40-41	34.549804807936226	38.0	35.0	41.0	22.5	41.0
42-43	34.19380114485773	38.0	34.5	41.0	19.5	41.0
44-45	33.77070207416948	38.0	33.5	41.0	14.5	41.0
46-47	33.082535683901654	38.0	33.0	40.0	5.0	41.0
48-49	32.00810909035535	38.0	31.5	40.0	2.0	41.0
50-51	31.08365759333976	37.5	30.0	40.0	2.0	41.0
52-53	30.73271408461013	38.0	29.5	40.0	2.0	41.0
54-55	30.417918456616576	37.0	28.5	40.0	2.0	41.0
56-57	29.972801028048302	37.0	27.5	40.0	2.0	41.0
58-59	29.55570899500276	36.0	26.0	40.0	2.0	41.0
60-61	29.102695180157237	36.0	25.5	40.0	2.0	41.0
62-63	28.62477423006043	35.0	23.5	39.5	2.0	41.0
64-65	28.083116488076918	35.0	21.5	39.0	2.0	41.0
66-67	27.53390135155527	35.0	19.0	39.0	2.0	41.0
68-69	26.92971329489317	34.0	13.0	38.0	2.0	40.0
70-71	26.329716105463373	34.0	7.5	37.5	2.0	40.0
72-73	25.719028205716945	34.0	2.0	37.0	2.0	39.5
74-75	25.090370861161215	33.0	2.0	36.0	2.0	39.0
76-77	24.446625532911682	32.0	2.0	36.0	2.0	39.0
78-79	24.103521870058785	32.0	2.0	35.0	2.0	38.0
80-81	23.544710860945642	32.0	2.0	35.0	2.0	37.0
82-83	22.92159044935033	31.0	2.0	35.0	2.0	37.0
84-85	22.39950983279708	31.0	2.0	35.0	2.0	36.0
86-87	21.807139409169892	30.5	2.0	35.0	2.0	36.0
88-89	21.163404906472245	29.5	2.0	34.5	2.0	35.5
90-91	20.493203652726073	29.0	2.0	34.0	2.0	35.0
92-93	19.87551950115857	28.5	2.0	34.0	2.0	35.0
94-95	19.138804051306913	26.5	2.0	34.0	2.0	35.0
96-97	18.4130651157416	25.0	2.0	34.0	2.0	35.0
98-99	17.700757964096894	22.0	2.0	34.0	2.0	35.0
100	16.737407862176905	18.0	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1750287415913725
1101	2	0.1949047857193662
1101	3	0.24483366792994232
1101	4	0.2542219176807805
1101	5	0.26846334559783713
1101	6	0.28742784668146726
1101	7	0.30926913918349896
1101	8	0.3121013411040252
1101	9	0.35153952176473524
1101	10-11	0.3310815364847457
1101	12-13	0.3236296711576401
1101	14-15	0.3783031803287926
1101	16-17	0.3344823737241356
1101	18-19	0.30885636734757327
1101	20-21	0.2538974647656218
1101	22-23	0.2524886741500936
1101	24-25	0.25047577034328583
1101	26-27	0.3186454349927388
1101	28-29	0.3788437433145049
1101	30-31	0.38783107586824883
1101	32-33	0.3971383616760704
1101	34-35	0.35486678656337034
1101	36-37	0.3178996709037136
1101	38-39	0.23675809545157023
1101	40-41	0.2635127436634832
1101	42-43	0.22201751425430416
1101	44-45	0.1836866264301591
1101	46-47	0.19957660232044105
1101	48-49	0.20712461607426036
1101	50-51	0.12936980579532786
1101	52-53	0.03116530177944199
1101	54-55	-0.011954423987006635
1101	56-57	-0.06758104618691618
1101	58-59	-0.12296038680573318
1101	60-61	-0.11731369405414327
1101	62-63	-0.23729911135418646
1101	64-65	-0.22666561585029754
1101	66-67	-0.30679041558629194
1101	68-69	-0.3539973932301663
1101	70-71	-0.43209901697981223
1101	72-73	-0.5370851772956087
1101	74-75	-0.5457213023046634
1101	76-77	-0.6293135600902744
1101	78-79	-0.7223840516157054
1101	80-81	-0.714404166590775
1101	82-83	-0.6997017431822918
1101	84-85	-0.8031403651737747
1101	86-87	-0.916532902656968
1101	88-89	-1.0411870703957007
1101	90-91	-1.1252077559012221
1101	92-93	-1.2241103059564473
1101	94-95	-1.3245437464160474
1101	96-97	-1.5246924111741365
1101	98-99	-1.5663410263400337
1101	100	-1.470578300976845
1102	1	0.1438326213347274
1102	2	0.20128435110962783
1102	3	0.2214547220921972
1102	4	0.23209406868257787
1102	5	0.24210260806267314
1102	6	0.2842983487189059
1102	7	0.27241244282317467
1102	8	0.2856736555525714
1102	9	0.30254556259582444
1102	10-11	0.30122961871984444
1102	12-13	0.2942311351917084
1102	14-15	0.35047360912770387
1102	16-17	0.3235511679642258
1102	18-19	0.34052863611076134
1102	20-21	0.30941773142321694
1102	22-23	0.2835904199537751
1102	24-25	0.3106303937132395
1102	26-27	0.3480870069605686
1102	28-29	0.3593096264226219
1102	30-31	0.33845385989815924
1102	32-33	0.355489148235435
1102	34-35	0.3811751249351758
1102	36-37	0.3557234430115983
1102	38-39	0.3300345151857016
1102	40-41	0.3123300203634969
1102	42-43	0.27147614988060553
1102	44-45	0.22084798072945233
1102	46-47	0.21132866734037492
1102	48-49	0.20467267892675522
1102	50-51	0.24212212170154856
1102	52-53	0.18680921203452883
1102	54-55	0.10270928748610686
1102	56-57	0.10333863415663203
1102	58-59	0.03429349394741621
1102	60-61	0.02040150287424325
1102	62-63	-0.06452500716398646
1102	64-65	-0.04897141103312208
1102	66-67	-0.08492501040436196
1102	68-69	-0.12557805646225617
1102	70-71	-0.14814247203535302
1102	72-73	-0.20967535786585145
1102	74-75	-0.22203665602340905
1102	76-77	-0.3084855723890989
1102	78-79	-0.3550737106790933
1102	80-81	-0.4108765879130054
1102	82-83	-0.42812954420691796
1102	84-85	-0.5340583057429669
1102	86-87	-0.6201340953061596
1102	88-89	-0.678344083449069
1102	90-91	-0.7570092686071774
1102	92-93	-0.8227389618842871
1102	94-95	-0.9279266064215399
1102	96-97	-1.0702933833882682
1102	98-99	-1.0857921658412728
1102	100	-1.012328931657878
1103	1	0.010493338321531098
1103	2	-0.003091054071600041
1103	3	-0.005800154331421226
1103	4	0.01261561060436378
1103	5	0.03894893935520827
1103	6	0.07596337716671542
1103	7	0.07407112415889827
1103	8	0.10639636712599554
1103	9	0.03152933255331902
1103	10-11	0.09270827197923381
1103	12-13	0.0667967911030587
1103	14-15	0.12278121328409242
1103	16-17	0.09585692102585597
1103	18-19	0.10699794262653484
1103	20-21	0.09803736176092315
1103	22-23	0.1234223689831353
1103	24-25	0.09673077730197122
1103	26-27	0.05557241138986768
1103	28-29	0.1037441202600462
1103	30-31	0.04406056158644134
1103	32-33	0.026339406530965448
1103	34-35	0.04797521132494609
1103	36-37	0.04520900885375312
1103	38-39	-0.039735768110773506
1103	40-41	-0.0075119797445069025
1103	42-43	0.052007478649230166
1103	44-45	-0.01405235888579881
1103	46-47	-0.07984554834287394
1103	48-49	-0.1603001288528496
1103	50-51	-0.11085780468985007
1103	52-53	-0.062263437950004885
1103	54-55	-0.11069403147573453
1103	56-57	-0.09144567135681214
1103	58-59	-0.14831223543004768
1103	60-61	-0.19026670239973242
1103	62-63	-0.27273607416454126
1103	64-65	-0.3142861126596159
1103	66-67	-0.2726023443560237
1103	68-69	-0.2862976135655124
1103	70-71	-0.2704310819778222
1103	72-73	-0.35492231169365596
1103	74-75	-0.3318455505779454
1103	76-77	-0.3121019441597781
1103	78-79	-0.3739573149291182
1103	80-81	-0.3767909540362808
1103	82-83	-0.3349709264865659
1103	84-85	-0.3964883838944715
1103	86-87	-0.5209763770863169
1103	88-89	-0.4627391226958011
1103	90-91	-0.4816672585768771
1103	92-93	-0.49927519841992307
1103	94-95	-0.5833355898047543
1103	96-97	-0.7246180594920482
1103	98-99	-0.7282644952348356
1103	100	-0.7149075706295136
1104	1	-0.047478400518478736
1104	2	-0.01830731554141707
1104	3	-0.04073064204228416
1104	4	0.03210067391778182
1104	5	-0.04683123799722466
1104	6	-0.03890007034711829
1104	7	-0.006842388563384816
1104	8	-0.03823655682788285
1104	9	-0.055466363488832826
1104	10-11	-0.040778468954783875
1104	12-13	-0.012736343456609234
1104	14-15	0.07730295344249072
1104	16-17	0.05283560792729247
1104	18-19	0.10340499296556516
1104	20-21	0.07914388184864407
1104	22-23	0.05062120755990662
1104	24-25	-0.0824283384472082
1104	26-27	0.01317293097428518
1104	28-29	0.040593144205566034
1104	30-31	0.0110887996364184
1104	32-33	0.052205206384485336
1104	34-35	0.09220079912976331
1104	36-37	0.03224091549640917
1104	38-39	0.08263525001474648
1104	40-41	0.036903860722532045
1104	42-43	0.008174352153538678
1104	44-45	0.07105343022212907
1104	46-47	-0.027779689485029735
1104	48-49	0.12509987897981745
1104	50-51	0.024588893170587767
1104	52-53	0.045491760741832366
1104	54-55	0.10657586259160468
1104	56-57	0.08799578320287438
1104	58-59	0.07265214750549376
1104	60-61	0.09048307842562764
1104	62-63	0.05832579636752655
1104	64-65	0.12939495097452536
1104	66-67	0.0702442508732588
1104	68-69	0.08469958122777754
1104	70-71	0.15169942833301064
1104	72-73	0.06478381605946382
1104	74-75	0.168637668509934
1104	76-77	0.13460979885783786
1104	78-79	0.08148501623384874
1104	80-81	0.1018845174717562
1104	82-83	0.037447696285966
1104	84-85	0.10189180200704584
1104	86-87	0.04302981813607332
1104	88-89	0.1002430683766633
1104	90-91	0.01850474690685644
1104	92-93	0.03965707086604553
1104	94-95	0.046332675275991164
1104	96-97	-0.08364287639674828
1104	98-99	-0.09365689822158885
1104	100	-0.025963562572698606
1105	1	-0.001945053895891391
1105	2	6.306587765187999E-4
1105	3	0.0011473285961436375
1105	4	0.007881845000817123
1105	5	-0.022082069823994743
1105	6	0.049016742270914904
1105	7	-0.0025276345509723797
1105	8	-8.690480218263019E-4
1105	9	0.05172476165025586
1105	10-11	0.03471785331083055
1105	12-13	0.09654462713534429
1105	14-15	0.0697575786509006
1105	16-17	0.04683141715464956
1105	18-19	0.04390523503359134
1105	20-21	0.027458907167364544
1105	22-23	0.03354538592093803
1105	24-25	0.04564823978637378
1105	26-27	0.04021905511091717
1105	28-29	-0.00907200231040406
1105	30-31	0.03660063019494686
1105	32-33	0.05674056420730267
1105	34-35	-0.022579096367145723
1105	36-37	0.008386904983304078
1105	38-39	0.03499207316431807
1105	40-41	0.11702153737265064
1105	42-43	0.11466614027671085
1105	44-45	0.14956910602304418
1105	46-47	0.14524728496810013
1105	48-49	0.15782767809658793
1105	50-51	0.27003193286513394
1105	52-53	0.19606085248647886
1105	54-55	0.20030948387162084
1105	56-57	0.08608082517541504
1105	58-59	0.22754899493676817
1105	60-61	0.19169888475699892
1105	62-63	0.21489757112078323
1105	64-65	0.2339144668327151
1105	66-67	0.26342205838222554
1105	68-69	0.315547887298937
1105	70-71	0.35103188639023486
1105	72-73	0.24148308269225538
1105	74-75	0.24520119845128008
1105	76-77	0.3024843364704637
1105	78-79	0.3097907570772236
1105	80-81	0.3334522300720053
1105	82-83	0.3581791231583793
1105	84-85	0.40183734510360125
1105	86-87	0.3782955216389432
1105	88-89	0.44579152312617154
1105	90-91	0.451178834720416
1105	92-93	0.4217795419705581
1105	94-95	0.3940399451807792
1105	96-97	0.311043818590246
1105	98-99	0.3411922535993277
1105	100	0.3862738862819093
1106	1	-0.22547645387102477
1106	2	-0.1854230824998524
1106	3	-0.17458875900010185
1106	4	-0.11438947750278317
1106	5	-0.13853398959773244
1106	6	-0.093994470539009
1106	7	-0.16108946071523178
1106	8	-0.14059337311784503
1106	9	-0.20585356144302835
1106	10-11	-0.14485883211166595
1106	12-13	-0.12658620684887012
1106	14-15	-0.13524516552211452
1106	16-17	-0.14546401403765685
1106	18-19	-0.13178491152987704
1106	20-21	-0.13569189143579052
1106	22-23	-0.13147072358784584
1106	24-25	-0.1419399934569867
1106	26-27	-0.1542603654362651
1106	28-29	-0.21299529852257137
1106	30-31	-0.22828139879658949
1106	32-33	-0.1983502621735198
1106	34-35	-0.11077505179590474
1106	36-37	-0.17013713735649105
1106	38-39	-0.13529104660113944
1106	40-41	-0.12615467380278744
1106	42-43	-0.09981267875054556
1106	44-45	-0.08457325762832824
1106	46-47	-0.09239186727073445
1106	48-49	-0.07199602191004573
1106	50-51	0.03820777710192047
1106	52-53	0.0130020806356832
1106	54-55	-0.02218240483342271
1106	56-57	0.028386170612279216
1106	58-59	0.05062085714935449
1106	60-61	0.05468911954216438
1106	62-63	-0.008500467906767284
1106	64-65	0.050995511998046084
1106	66-67	0.05642003531544049
1106	68-69	0.09721589258321828
1106	70-71	0.1850705220589397
1106	72-73	0.15311681413010803
1106	74-75	0.24121471964395624
1106	76-77	0.2655980131280913
1106	78-79	0.29123344541778096
1106	80-81	0.293476339634811
1106	82-83	0.33602707269448473
1106	84-85	0.30920918253034557
1106	86-87	0.3162773010551483
1106	88-89	0.33444719537030565
1106	90-91	0.36632182661162105
1106	92-93	0.25179294650287076
1106	94-95	0.33353532222294646
1106	96-97	0.2181359976939241
1106	98-99	0.22427722876232892
1106	100	0.20207072191628228
1107	1	-0.28379800947077527
1107	2	-0.28112713885386853
1107	3	-0.29198679557226725
1107	4	-0.23152430603947494
1107	5	-0.2686862502361862
1107	6	-0.3712562223214704
1107	7	-0.300472574338535
1107	8	-0.23248646518894844
1107	9	-0.314379670772297
1107	10-11	-0.2796158474520922
1107	12-13	-0.26021216699044913
1107	14-15	-0.330213181720417
1107	16-17	-0.323189608102993
1107	18-19	-0.2640233678287629
1107	20-21	-0.28658668083663486
1107	22-23	-0.30799550186845437
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2208	3	-0.6812116769927812
2208	4	-0.5789369574747809
2208	5	-0.7983953181284136
2208	6	-0.6911868647619386
2208	7	-0.6964899398413777
2208	8	-0.6594132288133636
2208	9	-0.7972625132545232
2208	10-11	-0.7666541564380012
2208	12-13	-0.8011260409416963
2208	14-15	-0.8949353410733991
2208	16-17	-0.8819836587373828
2208	18-19	-0.8900110425952974
2208	20-21	-0.9169929087236781
2208	22-23	-0.8692705759008206
2208	24-25	-0.9936512623045957
2208	26-27	-0.9672312461602743
2208	28-29	-1.080284491736876
2208	30-31	-1.1659284321561927
2208	32-33	-1.160110421821237
2208	34-35	-1.077884892563894
2208	36-37	-1.1116835562002194
2208	38-39	-1.0742720542468192
2208	40-41	-1.0505831955813107
2208	42-43	-1.113786298795226
2208	44-45	-1.0928716430569025
2208	46-47	-1.0752765313060522
2208	48-49	-0.9732010651418719
2208	50-51	-0.9471852837093984
2208	52-53	-0.8642869443222274
2208	54-55	-0.7877549387505454
2208	56-57	-0.8025498773217166
2208	58-59	-1.2692397969610738
2208	60-61	-0.894506485023836
2208	62-63	-0.7975690449455435
2208	64-65	-0.7026050128144199
2208	66-67	-0.6029945063633235
2208	68-69	-0.5628826111864456
2208	70-71	-0.41303840125192437
2208	72-73	-0.30190219015121755
2208	74-75	-0.3139741474024582
2208	76-77	-0.23464689766925417
2208	78-79	-0.20010218125138834
2208	80-81	-0.15482485806073143
2208	82-83	-0.0416633027676383
2208	84-85	0.00694300509475454
2208	86-87	0.03919772059235882
2208	88-89	0.09098219083875136
2208	90-91	-0.7158849153495126
2208	92-93	-0.31298121144899227
2208	94-95	-0.03311262564698225
2208	96-97	0.4062421928954407
2208	98-99	0.5203619895537095
2208	100	0.44019234854016176
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	466279.0
3	133130.0
4	109346.0
5	107096.0
6	113113.0
7	125496.0
8	136013.0
9	149593.0
10	170970.0
11	193463.0
12	222080.0
13	270214.0
14	352699.0
15	454997.0
16	555213.0
17	698365.0
18	1072905.0
19	831843.0
20	549834.0
21	475163.0
22	471323.0
23	487889.0
24	517706.0
25	555381.0
26	596425.0
27	646463.0
28	708018.0
29	780321.0
30	866335.0
31	972326.0
32	1110076.0
33	1296725.0
34	1572946.0
35	2017481.0
36	2740042.0
37	3957079.0
38	4363043.0
39	1066453.0
40	1388.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.78914450227534	35.115115798841735	25.572526332386435	20.52321336649649
2	21.258573293787304	25.81904850839755	33.46646764460577	19.455910553209375
3	21.455173290027133	33.74170888419303	23.895897550129234	20.907220275650594
4	21.95005826939809	23.69054115129818	33.27997577834628	21.07942480095745
5	27.1963567495839	24.05864286494371	23.92663503806642	24.818365347405972
6	27.203542046061408	24.082337919918412	27.37705964775701	21.337060386263172
7	22.303737353670066	29.328646253327378	23.47627949378884	24.891336899213716
8	26.108713105491194	24.252721008958318	28.604993859110106	21.033572026440382
9	21.575855831117956	28.251246080578575	23.789319989981827	26.383578098321642
10-11	28.334724281025586	24.08317463375311	23.763475816309146	23.818625268912157
12-13	25.855180121197176	24.179046970640268	23.940168025159096	26.02560488300346
14-15	25.515281886395925	24.36090897904345	24.490585503447438	25.63322363111319
16-17	25.224991593438258	24.295514297517578	24.838694860308777	25.640799248735384
18-19	24.87923926496281	24.24816915996829	25.598243351424653	25.274348223644242
20-21	22.204886580279254	26.272307628415543	28.829971109294938	22.692834682010265
22-23	22.16640959107539	26.29791465533342	29.161379856696907	22.374295896894285
24-25	19.685642165292368	23.83246075530174	32.00707647078948	24.47482060861642
26-27	19.71563884497359	23.88910435199432	36.81380211210256	19.581454690929537
28-29	19.710456054787656	23.95751792657596	36.798094663979484	19.533931354656897
30-31	19.696143758569985	23.92888546160066	36.780645937903465	19.59432484192589
32-33	19.726690594441454	23.97111273554638	36.69880698026625	19.603389689745924
34-35	19.762993766707485	24.040963548190238	36.69204512675449	19.503997558347795
36-37	19.782528380816164	24.0267392339887	36.59009501684878	19.60063736834636
38-39	19.819612996242395	24.10739564337801	36.45163955839708	19.621351801982513
40-41	19.873802046575868	24.239654635327714	36.23244986941233	19.654093448684094
42-43	20.008798041612984	24.323315962137706	35.837951168988454	19.82993482726086
44-45	20.117906539500567	24.549899448126357	35.39186175418516	19.94033225818792
46-47	20.42458188215205	24.840435684352403	34.65105506769766	20.083927365797887
48-49	20.85342682590015	25.454648570425263	33.11523384487586	20.57669075879873
50-51	21.330603363990274	26.374122162293933	31.205327307818315	21.089947165897478
52-53	21.65356820184614	26.837816583234254	30.0835967493289	21.425018465590707
54-55	21.814659510591575	26.93325849979025	29.47708181214082	21.77500017747736
56-57	21.936269691444156	27.089719572770644	29.089794447431196	21.884216288354008
58-59	22.06022151691554	27.194573393105102	28.845347990268227	21.89985709971113
60-61	22.06394270049144	27.200492109182495	28.703886601799482	22.031678588526578
62-63	22.15193338215256	27.233947398759145	28.65869790788119	21.9554213112071
64-65	22.233201481944683	27.305205851797638	28.545056368834445	21.916536297423242
66-67	22.2914038825413	27.313145945230143	28.43028024549677	21.96516992673179
68-69	22.393339499024677	27.43643546285131	28.19356169831506	21.97666333980895
70-71	22.579359442025417	27.570661694752456	27.966807439581704	21.883171423640416
72-73	22.68018624885089	27.702445091230636	27.596758915492615	22.020609744425855
74-75	22.827389645987996	27.882200972073196	27.359091051110823	21.931318330827985
76-77	22.738756628058685	28.15852714891072	27.036117376537668	22.066598846492923
78-79	22.829736443258167	28.25010466174548	26.82845836104713	22.091700533949226
80-81	22.91320540372312	28.38730972386343	26.60151779528458	22.097967077128875
82-83	22.96639787705143	28.565120046530378	26.313247729928253	22.155234346489937
84-85	23.027023118991586	28.571189277159796	26.119625537464664	22.282162066383954
86-87	23.136432616055274	28.676507873807715	25.86637991256221	22.320679597574795
88-89	23.289710085554123	28.711727472808096	25.604864480867345	22.393697960770435
90-91	23.359504899805124	28.70280217867881	25.30463630344545	22.63305661807061
92-93	23.511209955674243	28.715547747173765	25.196868000108637	22.576374297043362
94-95	23.62234147906462	28.783075830330013	24.9032148752843	22.691367815321065
96-97	23.676059576897618	28.82237802196527	24.64770490701763	22.853857494119485
98-99	24.02061502352261	28.696181663404154	24.45648654912171	22.826716763951527
100	24.96324050382687	28.39649638751776	23.57786429702557	23.062398811629794
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10362.0
1	7090.5
2	3595.5
3	3312.0
4	3280.0
5	3364.0
6	3569.0
7	3958.0
8	4599.0
9	5430.0
10	6549.5
11	7992.0
12	9721.0
13	12979.5
14	19695.0
15	27874.0
16	109542.5
17	140442.5
18	93519.5
19	97224.5
20	110390.5
21	122696.0
22	133781.5
23	151034.5
24	171360.0
25	195969.0
26	224801.0
27	258606.0
28	297020.0
29	339418.0
30	383894.5
31	434850.0
32	495440.5
33	559584.5
34	622406.5
35	695179.0
36	780942.0
37	877679.5
38	969906.5
39	1034897.5
40	1083533.0
41	1137576.5
42	1213416.0
43	1277860.5
44	1316782.5
45	1329233.0
46	1305734.0
47	1256391.5
48	1195868.0
49	1151918.5
50	1097158.0
51	1021451.0
52	937687.5
53	845440.0
54	766473.0
55	698891.0
56	625845.0
57	556492.0
58	498101.0
59	444870.5
60	396119.5
61	347261.0
62	301820.0
63	263223.5
64	229198.0
65	198873.5
66	170975.5
67	146207.5
68	123519.0
69	103351.5
70	86149.0
71	70741.5
72	58024.5
73	47835.5
74	39111.5
75	31626.0
76	25419.0
77	20236.5
78	16127.5
79	12673.5
80	9686.5
81	7497.0
82	5754.0
83	4254.5
84	3047.5
85	2177.0
86	1542.5
87	1021.0
88	714.0
89	516.0
90	335.5
91	239.5
92	171.5
93	106.0
94	66.0
95	43.5
96	24.5
97	18.0
98	14.0
99	7.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1810702801721761
2	0.0771731817584782
3	0.07289309380549075
4	0.09309974622775732
5	0.10112412781458083
6	0.04071096835517285
7	0.039159984799734496
8	0.03210692624762997
9	0.04136582807858016
10-11	0.037359903885392405
12-13	0.031177902764422956
14-15	0.031564865328254546
16-17	0.031632231280662476
18-19	0.03325998068884475
20-21	0.032921584276749113
22-23	0.02998881537192022
24-25	0.03152883237696658
26-27	0.022613026908280036
28-29	0.021774869128320922
30-31	0.02075184664175401
32-33	0.02101504385116173
34-35	0.0178331775874291
36-37	0.021427072815889292
38-39	0.02610822318321233
40-41	0.024256442817022293
42-43	0.03260042101526945
44-45	0.03307354933218095
46-47	0.03327721383946073
48-49	0.029390355050528852
50-51	0.02862269652308967
52-53	0.02903472548781723
54-55	0.030560642642359612
56-57	0.026872748410539517
58-59	0.02183596848050486
60-61	0.011325313254811998
62-63	0.009221302229606227
64-65	0.006360599227353258
66-67	0.007994615235759527
68-69	0.013282059174753922
70-71	0.020037454216218765
72-73	0.017753278434573184
74-75	0.017217484115421754
76-77	0.022942023420039686
78-79	0.02032415117646646
80-81	0.012569233399274678
82-83	0.01534377064844774
84-85	0.018564803163580324
86-87	0.022841757816455793
88-89	0.02617402248556426
90-91	0.031121503362407016
92-93	0.0881397321504666
94-95	0.13863129680523706
96-97	0.09153779612192699
98-99	0.07253903089283513
100	0.07780924168121353
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.1915232E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.893219723414006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.50561106356544	33.580775964871314
2	11.976111376119865	10.513401761282148
3	4.381305059211596	5.7692875691784
4	2.178497327858274	3.82485047514214
5	1.2466624450743333	2.7360014311288126
6	0.8000372071386195	2.106972535190514
7	0.5292759424348413	1.626213766492666
8	0.3927136642443595	1.3789973722451765
9	0.2847354614326561	1.1248160554540123
>10	1.4447607962742155	11.118465859384818
>50	0.10633544041730833	3.3367250997676177
>100	0.14896710782112524	13.644552372653404
>500	0.00431426056220035	1.154231565886449
>1k	3.551934071066658E-4	0.28358892829367705
>5k	8.786186600745085E-5	0.27368085772722045
>10k+	2.297925721409712E-4	7.527438385301596
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	956764	2.997828748354391	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	686007	2.149465809930506	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	99303	0.3111461010216062	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA	69087	0.2164703048375146	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	58209	0.1823862662192147	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	56276	0.1763295971027251	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	48524	0.15204025463452686	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA	47393	0.1484964922078586	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG	38803	0.12158144424580715	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	36362	0.11393305867242326	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34079	0.10677973451673484	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33489	0.1049310874506568	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.399900335990038E-6	0.0	0.0	0.0	0.0
2	9.399900335990038E-6	0.0	0.0	3.1333001119966793E-6	0.0
3	9.399900335990038E-6	3.1333001119966793E-6	0.0	6.266600223993359E-6	0.0
4	9.399900335990038E-6	3.1333001119966793E-6	0.0	9.399900335990038E-6	0.0
5	9.399900335990038E-6	9.399900335990038E-6	0.0	9.399900335990038E-6	0.0
6	9.399900335990038E-6	9.399900335990038E-6	0.0	1.2533200447986717E-5	0.0
7	1.2533200447986717E-5	9.399900335990038E-6	0.0	1.5666500559983395E-5	0.0
8	1.2533200447986717E-5	1.2533200447986717E-5	0.0	1.5666500559983395E-5	0.0
9	1.5666500559983395E-5	1.5666500559983395E-5	0.0	1.8799800671980076E-5	0.0
10-11	1.8799800671980073E-5	1.5666500559983395E-5	0.0	2.0366450727978413E-5	1.5666500559983397E-6
12-13	2.8199701007970112E-5	1.8799800671980076E-5	0.0	2.3499750839975094E-5	3.1333001119966793E-6
14-15	4.073290145595683E-5	2.3499750839975094E-5	0.0	2.5066400895973435E-5	6.266600223993359E-6
16-17	7.206590257592362E-5	3.603295128796181E-5	0.0	3.133300111996679E-5	6.266600223993359E-6
18-19	1.3786520492785388E-4	4.073290145595683E-5	0.0	3.446630123196347E-5	6.266600223993359E-6
20-21	2.099311075037775E-4	5.013280179194687E-5	0.0	3.759960134396015E-5	6.266600223993359E-6
22-23	4.684283667435035E-4	5.4832751959941884E-5	0.0	4.386620156795351E-5	7.8332502799917E-6
24-25	8.271912295671232E-4	5.6399402015940225E-5	0.0	4.543285162395185E-5	9.399900335990038E-6
26-27	0.001259586645022665	5.6399402015940225E-5	3.1333001119966793E-6	4.856615173594853E-5	9.399900335990038E-6
28-29	0.0020397783729098383	5.6399402015940225E-5	3.1333001119966793E-6	5.013280179194687E-5	9.399900335990038E-6
30-31	0.0027087379468211294	6.73659524079286E-5	3.1333001119966793E-6	5.4832751959941884E-5	9.399900335990038E-6
32-33	0.003123900211660689	8.146580291191367E-5	3.1333001119966793E-6	5.6399402015940225E-5	9.399900335990038E-6
34-35	0.0038382926371959324	8.146580291191367E-5	3.1333001119966793E-6	6.266600223993358E-5	9.399900335990038E-6
36-37	0.004949047526898755	8.773240313590701E-5	3.1333001119966793E-6	6.579930235193026E-5	9.399900335990038E-6
38-39	0.006500031082337111	1.0339890369589042E-4	3.1333001119966793E-6	6.73659524079286E-5	9.399900335990038E-6
40-41	0.00903017092277443	1.112321539758821E-4	3.1333001119966793E-6	6.893260246392694E-5	9.399900335990038E-6
42-43	0.012411001743618845	1.1906540425587381E-4	3.1333001119966793E-6	6.893260246392694E-5	9.399900335990038E-6
44-45	0.017220617415533747	1.2533200447986716E-4	3.1333001119966793E-6	7.363255263192196E-5	9.399900335990038E-6
46-47	0.02385538040268672	1.347319048158572E-4	3.1333001119966793E-6	7.51992026879203E-5	9.399900335990038E-6
48-49	0.032575354614373477	1.4413180515184725E-4	3.1333001119966793E-6	7.833250279991698E-5	1.2533200447986717E-5
50-51	0.044251597481729105	1.4883175531984225E-4	6.266600223993359E-6	7.989915285591532E-5	1.2533200447986717E-5
52-53	0.059852298739360564	1.582316556558323E-4	6.266600223993359E-6	8.459910302391033E-5	1.2533200447986717E-5
54-55	0.08000098510955521	1.6763155599182236E-4	6.266600223993359E-6	8.459910302391033E-5	1.4099850503985056E-5
56-57	0.10650087080676712	1.7233150615981736E-4	6.266600223993359E-6	8.459910302391033E-5	1.7233150615981735E-5
58-59	0.13998331580356363	1.7233150615981736E-4	6.266600223993359E-6	8.459910302391033E-5	1.8799800671980076E-5
60-61	0.18206823625784704	1.738981562158157E-4	6.266600223993359E-6	9.08657032479037E-5	1.8799800671980076E-5
62-63	0.23373635510467228	1.8643135666380242E-4	6.266600223993359E-6	9.399900335990038E-5	1.8799800671980076E-5
64-65	0.2973345141279249	1.973979070557908E-4	6.266600223993359E-6	9.713230347189706E-5	1.8799800671980076E-5
66-67	0.37586754813500967	2.0366450727978414E-4	1.0966550391988377E-5	1.0026560358389374E-4	1.8799800671980076E-5
68-69	0.46816203623398384	2.0366450727978414E-4	1.4099850503985056E-5	1.0026560358389374E-4	1.8799800671980076E-5
70-71	0.57554806432239	2.0366450727978414E-4	1.7233150615981735E-5	1.0183225363989207E-4	1.8799800671980076E-5
72-73	0.70210832244616	2.1149775755977584E-4	2.3499750839975094E-5	1.0653220380788709E-4	1.8799800671980076E-5
74-75	0.847266283384686	2.1306440761577417E-4	2.8199701007970112E-5	1.0653220380788709E-4	2.0366450727978413E-5
76-77	1.015184536336756	2.1619770772777087E-4	2.8199701007970112E-5	1.0966550391988377E-4	2.1933100783976754E-5
78-79	1.2065273409261135	2.2403095800776256E-4	2.8199701007970112E-5	1.0966550391988377E-4	2.3499750839975094E-5
80-81	1.4226639493017001	2.3499750839975093E-4	2.8199701007970112E-5	1.1279880403188045E-4	2.5066400895973435E-5
82-83	1.6610939879741435	2.44397408735741E-4	2.8199701007970112E-5	1.1906540425587381E-4	2.6633050951971775E-5
84-85	1.9231914090425537	2.4753070884773765E-4	2.8199701007970112E-5	1.2376535442386883E-4	3.133300111996679E-5
86-87	2.2081681875287638	2.553639591277294E-4	2.8199701007970112E-5	1.2533200447986716E-4	3.289965117596513E-5
88	2.4354233113517707	2.6946380963171443E-4	2.8199701007970112E-5	1.2533200447986716E-4	3.446630123196347E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	145155	0.0	87.818825	2
ATATCTC	148380	0.0	86.46154	1
TATGGAT	176270	0.0	84.24548	2
GGATCCG	175975	0.0	83.52193	5
ATGGATC	177995	0.0	83.13035	3
ATCTCGA	155000	0.0	81.67948	3
ATATGGA	182970	0.0	81.67233	1
TGGATCC	181425	0.0	81.169754	4
TCTCGAG	159360	0.0	79.27644	4
GATCCGG	186725	0.0	78.78771	6
CTCGAGG	166425	0.0	75.89084	5
ATCCGGC	194415	0.0	75.519455	7
TCGAGGG	172665	0.0	73.1127	6
TCCGGCG	202735	0.0	72.70371	8
CCGGCGC	214375	0.0	69.20769	9
CGAGGGC	188305	0.0	66.96144	7
AGGGCGC	195800	0.0	64.55005	9
GAGGGCG	200355	0.0	63.052036	8
CCGTCGA	190230	0.0	36.378235	16-17
GTCGACT	191330	0.0	36.111595	18-19
>>END_MODULE