FastQCFastQC Report
Wed 25 May 2016
SRR490979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28271452
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT13548114.792152168201336No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT11142753.941343373520398No Hit
ATATGGATCCGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1121290.39661563898451346No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT994440.3517470556517578No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA768140.27170164447160333No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT664410.23501092197174733No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA647880.22916403444718722No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC641640.22695686093519357No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG552410.195394987141092No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC492420.17417570204742225No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA488620.17283158997281073No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450710.1594223034600416No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA443790.15697460462943327No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436070.15424393483574877No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434350.15363554726513515No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG433310.15326768501313623No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT402200.14226365168651403No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376020.13300342691984832No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA375470.1328088843827335No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343080.12135209751518952No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT338580.11976038584788641No Hit
TCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323850.11455018299024755No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318710.11273209455248355No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302130.10686752134273117No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG291120.10297313346339622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC2026300.089.416311
TATCTCG2045900.087.888222
TATGGAT2514900.086.660152
ATATGGA2532750.086.3753741
ATGGATC2545050.085.368533
TGGATCC2616450.082.6947254
GGATCCG2611800.082.6419755
ATCTCGA2226750.080.179263
TCTCGAG2298850.077.397794
GATCCGG2813150.076.7282266
CTCGAGG2417400.073.51985
ATCCGGC2931200.072.9530947
TCGAGGG2522950.070.334236
TCCGGCG3092300.069.11168
CCGGCGC3261400.065.525369
CGAGGGC2711150.065.195867
GAGGGCG2907250.060.8143128
AGGGCGC2916550.060.4785969
CCGTCGA2899900.033.35939816-17
GTCGACT2900350.033.20657318-19
Produced by FastQC (version 0.11.5)