##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490979_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28271452
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.055795683928793	31.0	31.0	34.0	28.0	34.0
2	31.336779448045327	33.0	31.0	34.0	28.0	34.0
3	31.459768886295617	34.0	31.0	34.0	28.0	34.0
4	34.82448305095897	37.0	35.0	37.0	32.0	37.0
5	34.64216089785555	37.0	35.0	37.0	32.0	37.0
6	34.5164613052064	37.0	35.0	37.0	30.0	37.0
7	34.504216161235725	37.0	35.0	37.0	31.0	37.0
8	34.373949346499785	37.0	35.0	37.0	30.0	37.0
9	35.84365100879856	39.0	37.0	39.0	30.0	39.0
10-11	35.74954487657726	39.0	37.0	39.0	30.0	39.0
12-13	35.57541384857063	39.0	36.0	39.0	30.0	39.0
14-15	36.59852422861054	40.0	37.0	41.0	29.5	41.0
16-17	36.47781829528954	40.0	36.5	41.0	29.5	41.0
18-19	36.41242092907007	40.0	36.5	41.0	28.5	41.0
20-21	35.90012034047632	39.5	36.0	41.0	27.0	41.0
22-23	35.739541145605116	39.0	36.0	41.0	26.5	41.0
24-25	35.859103045008084	39.0	36.0	41.0	27.0	41.0
26-27	35.911381364494474	39.0	35.5	41.0	27.5	41.0
28-29	35.923195508317015	40.0	35.0	41.0	28.0	41.0
30-31	35.61709432186221	39.5	35.0	41.0	27.0	41.0
32-33	34.74052241816233	38.0	35.0	40.0	25.5	41.0
34-35	34.3670457215993	38.0	35.0	40.0	24.0	41.0
36-37	34.05414092279378	38.0	34.0	40.0	21.5	41.0
38-39	33.720349471261684	37.5	34.0	40.0	18.5	41.0
40-41	33.327238763682885	37.0	33.0	40.0	14.0	41.0
42-43	32.86825549320919	37.0	33.0	40.0	8.0	41.0
44-45	32.34530964309863	37.0	33.0	40.0	2.0	41.0
46-47	31.530479474488963	36.0	31.5	40.0	2.0	41.0
48-49	30.21440936249047	36.0	28.0	40.0	2.0	41.0
50-51	29.03832273984371	35.5	23.5	40.0	2.0	41.0
52-53	28.539390813743843	35.0	21.0	40.0	2.0	41.0
54-55	28.115716447814567	35.0	16.5	40.0	2.0	41.0
56-57	27.60180297778834	35.0	11.0	40.0	2.0	41.0
58-59	27.16088789850624	35.0	7.5	39.5	2.0	41.0
60-61	26.725063555278307	35.0	7.0	39.0	2.0	41.0
62-63	26.253319638481955	34.0	4.0	39.0	2.0	41.0
64-65	25.735461305630853	34.0	2.0	38.5	2.0	40.5
66-67	25.25554260531083	33.0	2.0	38.0	2.0	40.0
68-69	24.729718516049335	33.0	2.0	37.0	2.0	40.0
70-71	24.203627903511993	32.5	2.0	36.5	2.0	39.5
72-73	23.65921928947972	32.0	2.0	36.0	2.0	39.0
74-75	23.08819301180569	31.0	2.0	35.0	2.0	39.0
76-77	22.49912673392226	31.0	2.0	35.0	2.0	37.5
78-79	22.19333152750697	31.0	2.0	35.0	2.0	37.0
80-81	21.66007982186412	30.0	2.0	35.0	2.0	37.0
82-83	21.055568405188385	29.5	2.0	35.0	2.0	36.0
84-85	20.536724785129536	29.0	2.0	35.0	2.0	36.0
86-87	19.933432195134515	28.5	2.0	34.0	2.0	35.0
88-89	19.2673261352123	26.5	2.0	34.0	2.0	35.0
90-91	18.5634384643562	25.0	2.0	34.0	2.0	35.0
92-93	17.90269150307526	22.0	2.0	34.0	2.0	35.0
94-95	17.138411939365547	16.0	2.0	34.0	2.0	35.0
96-97	16.40279121496837	2.0	2.0	33.5	2.0	35.0
98-99	15.713107360032303	2.0	2.0	33.0	2.0	35.0
100	14.875593054081552	2.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11197603529438993
1101	2	0.13733433800957684
1101	3	0.18262205900926531
1101	4	0.19376821923970766
1101	5	0.18666916379496
1101	6	0.21828011924992552
1101	7	0.2399948976455164
1101	8	0.2108938412887582
1101	9	0.2424202323916802
1101	10-11	0.19522781390262622
1101	12-13	0.13752639826729052
1101	14-15	0.12409420251932346
1101	16-17	0.10522800251408881
1101	18-19	0.09976265090104164
1101	20-21	-0.027185336920979353
1101	22-23	-7.321301414364711E-4
1101	24-25	0.016989905499741553
1101	26-27	0.10563651855601819
1101	28-29	0.19717321244559827
1101	30-31	0.19999506101503783
1101	32-33	0.22306051115465664
1101	34-35	0.18889354964483118
1101	36-37	0.1797153676302301
1101	38-39	0.08082215313712737
1101	40-41	0.11171045883719444
1101	42-43	0.03921506566227606
1101	44-45	0.018807701331880367
1101	46-47	0.027845186074678452
1101	48-49	0.05541542490500717
1101	50-51	-0.02627340254385757
1101	52-53	-0.1174852381000484
1101	54-55	-0.1317834066557637
1101	56-57	-0.16955109281643033
1101	58-59	-0.24512618363557692
1101	60-61	-0.26422720518541354
1101	62-63	-0.3688837114902874
1101	64-65	-0.3380071348845597
1101	66-67	-0.4084960897233785
1101	68-69	-0.44133609209223224
1101	70-71	-0.4953537484968358
1101	72-73	-0.6004238008879064
1101	74-75	-0.6198027539173196
1101	76-77	-0.6720644973449765
1101	78-79	-0.7654086115307273
1101	80-81	-0.7510057537802233
1101	82-83	-0.6947948400711077
1101	84-85	-0.7866670678083345
1101	86-87	-0.9160767354502077
1101	88-89	-1.0731012453584405
1101	90-91	-1.1714908997674875
1101	92-93	-1.2029187371753096
1101	94-95	-1.2983301227526916
1101	96-97	-1.4989980428478002
1101	98-99	-1.512888185485135
1101	100	-1.3715227659942073
1102	1	0.16542512718367774
1102	2	0.21301783239550076
1102	3	0.2286287914523264
1102	4	0.2381310053098602
1102	5	0.26298628752240205
1102	6	0.3212473553615425
1102	7	0.2687295771320706
1102	8	0.2905477234769407
1102	9	0.32971784714479213
1102	10-11	0.3039763681132541
1102	12-13	0.2557404377084893
1102	14-15	0.311434959065231
1102	16-17	0.26517431925937984
1102	18-19	0.29355816747531094
1102	20-21	0.2292473947044158
1102	22-23	0.23061820264701538
1102	24-25	0.2655366971725712
1102	26-27	0.29781528930092094
1102	28-29	0.3205224320221518
1102	30-31	0.27870653469415885
1102	32-33	0.30261746981561544
1102	34-35	0.3244503102840781
1102	36-37	0.3301011074882183
1102	38-39	0.2907469860317846
1102	40-41	0.27644314273348414
1102	42-43	0.2670534810684231
1102	44-45	0.21614866147633194
1102	46-47	0.1973784017526441
1102	48-49	0.1977357321906723
1102	50-51	0.22188958751432253
1102	52-53	0.11988319641322676
1102	54-55	0.09590332488792086
1102	56-57	0.09732558667787927
1102	58-59	0.02890920093484084
1102	60-61	0.008531621560003089
1102	62-63	-0.04873268010213394
1102	64-65	-0.05205708693046418
1102	66-67	-0.0996313967094764
1102	68-69	-0.10588647061068457
1102	70-71	-0.13996372222569065
1102	72-73	-0.20479683098905
1102	74-75	-0.1810469634752785
1102	76-77	-0.21339445095239284
1102	78-79	-0.2896436664426538
1102	80-81	-0.3276004604365532
1102	82-83	-0.3520163195930728
1102	84-85	-0.49386419288778427
1102	86-87	-0.5781443546708367
1102	88-89	-0.6453995879763319
1102	90-91	-0.6860555152826358
1102	92-93	-0.7530279292111786
1102	94-95	-0.8297416626470806
1102	96-97	-0.9520911043320357
1102	98-99	-0.9816763111373596
1102	100	-0.9179465114734899
1103	1	0.022681850445327
1103	2	-0.005045140843041196
1103	3	-0.006691124756493139
1103	4	-0.005182448724141864
1103	5	0.049461521198153946
1103	6	0.08308782891376154
1103	7	0.0750711162442741
1103	8	0.11622077061455371
1103	9	0.059104504531816815
1103	10-11	0.07436641210745876
1103	12-13	0.037122152154246635
1103	14-15	0.056753531722954165
1103	16-17	0.035675512767106454
1103	18-19	0.03346863695703206
1103	20-21	0.043890039250470636
1103	22-23	0.0794695486130621
1103	24-25	0.06093878262400665
1103	26-27	0.011936798450676633
1103	28-29	0.08435509716880318
1103	30-31	0.028208003315633334
1103	32-33	1.5663948214950096E-4
1103	34-35	0.04362096744441146
1103	36-37	0.025584866542182283
1103	38-39	-5.357670284595883E-4
1103	40-41	0.005249884315631448
1103	42-43	0.04711126194284532
1103	44-45	-0.0223980443267493
1103	46-47	-0.06424989489848087
1103	48-49	-0.08729069956760327
1103	50-51	-0.04926644558926796
1103	52-53	0.013642830956271723
1103	54-55	-0.04298256431933822
1103	56-57	-0.04779019199628465
1103	58-59	-0.1366892512069171
1103	60-61	-0.16452529506442914
1103	62-63	-0.25546581376103106
1103	64-65	-0.2989253468485167
1103	66-67	-0.2969574253943641
1103	68-69	-0.29413170799422517
1103	70-71	-0.2840640702248898
1103	72-73	-0.3539037327780683
1103	74-75	-0.33193888703400987
1103	76-77	-0.3043604309463461
1103	78-79	-0.3772845009512409
1103	80-81	-0.3560345060421284
1103	82-83	-0.2904619048157251
1103	84-85	-0.3463109892029159
1103	86-87	-0.4715171107438465
1103	88-89	-0.4434578578646544
1103	90-91	-0.4688189563055367
1103	92-93	-0.4619705728086103
1103	94-95	-0.5355942097394468
1103	96-97	-0.6461544795302601
1103	98-99	-0.6351877907904502
1103	100	-0.6110091897772847
1104	1	-0.03396196881559632
1104	2	-0.005813307981938465
1104	3	-0.02267254090890347
1104	4	0.03863228316524214
1104	5	-0.07616397187126722
1104	6	-0.016281641500611954
1104	7	-0.00202214441563342
1104	8	-0.04008747581607963
1104	9	-0.06888486014756978
1104	10-11	-0.06938456757315237
1104	12-13	-0.0326217339725261
1104	14-15	0.03581763845596697
1104	16-17	0.04978126442362196
1104	18-19	0.07280841117543702
1104	20-21	0.05099271634166769
1104	22-23	0.033458002173382795
1104	24-25	-0.10692886340610386
1104	26-27	0.008220651136319646
1104	28-29	0.023995784775202367
1104	30-31	-0.02480343106596905
1104	32-33	0.041080862648250616
1104	34-35	0.07579600293725264
1104	36-37	0.037606228530712826
1104	38-39	0.08571657311652103
1104	40-41	0.03094023984598948
1104	42-43	0.0022844255667493485
1104	44-45	0.056811553585063734
1104	46-47	-0.024563410963434507
1104	48-49	0.10668382781810593
1104	50-51	0.012299204995912305
1104	52-53	0.03132366384425822
1104	54-55	0.08483523976390472
1104	56-57	0.06391113105774338
1104	58-59	0.06016343667262802
1104	60-61	0.06364694185641895
1104	62-63	0.013416637422942301
1104	64-65	0.06562340777044184
1104	66-67	0.022894731404765167
1104	68-69	0.06747732637822068
1104	70-71	0.13688553823243055
1104	72-73	0.03981460379198509
1104	74-75	0.15895475039197038
1104	76-77	0.14731428391975498
1104	78-79	0.08026006104064365
1104	80-81	0.11930214011060158
1104	82-83	0.07670934884984248
1104	84-85	0.14802489529067486
1104	86-87	0.09195749448342383
1104	88-89	0.15200130886085006
1104	90-91	0.09086189982443216
1104	92-93	0.08502004682411979
1104	94-95	0.0752094011043205
1104	96-97	-0.03924995934540476
1104	98-99	-0.05801215504812163
1104	100	-0.010879250167953103
1105	1	-0.039185492675272826
1105	2	-0.02376271957888676
1105	3	-0.02162784203649437
1105	4	0.0018843409352342633
1105	5	-0.04026494279839454
1105	6	0.008576534814245917
1105	7	-0.014289230051616642
1105	8	-0.004746497021734797
1105	9	0.03365306983101135
1105	10-11	0.011648097250741785
1105	12-13	0.0514340002450453
1105	14-15	0.04449208142258243
1105	16-17	0.016605912032353842
1105	18-19	0.0030898101320175897
1105	20-21	0.02445236782927651
1105	22-23	0.02730846105928464
1105	24-25	0.019277139897205586
1105	26-27	0.01174473876369575
1105	28-29	-0.07056043847398286
1105	30-31	-0.011531060052000441
1105	32-33	0.007584838023703355
1105	34-35	-0.08110345428557508
1105	36-37	-0.045335416771784764
1105	38-39	-0.04203764721744818
1105	40-41	0.030160351456515855
1105	42-43	0.044678252210445635
1105	44-45	0.08094846274035916
1105	46-47	0.0642565124230643
1105	48-49	0.15368695318245784
1105	50-51	0.2887157313229878
1105	52-53	0.2296995665538475
1105	54-55	0.21905953574932013
1105	56-57	0.08672524628354594
1105	58-59	0.2523639416569523
1105	60-61	0.23904958047314295
1105	62-63	0.2505987091466899
1105	64-65	0.24300636798355058
1105	66-67	0.2821303769678387
1105	68-69	0.28542339578434195
1105	70-71	0.28763057707649153
1105	72-73	0.21527640070108944
1105	74-75	0.21958104636451026
1105	76-77	0.2925104465809554
1105	78-79	0.30751102654320306
1105	80-81	0.29389577599095773
1105	82-83	0.3403420361324585
1105	84-85	0.41593447516750714
1105	86-87	0.406085921265003
1105	88-89	0.4589594734249154
1105	90-91	0.4474507238504053
1105	92-93	0.42599735178298914
1105	94-95	0.40229055896291044
1105	96-97	0.3567146563547752
1105	98-99	0.38497944645977
1105	100	0.4033240981519004
1106	1	-0.22324980461874233
1106	2	-0.193554564965023
1106	3	-0.18689761053695264
1106	4	-0.1199263758316178
1106	5	-0.14851257031257603
1106	6	-0.10966607818978957
1106	7	-0.1620002107361529
1106	8	-0.1572750543755035
1106	9	-0.20480548180891134
1106	10-11	-0.12598092935589023
1106	12-13	-0.10703784126003058
1106	14-15	-0.11329249071118142
1106	16-17	-0.12514264387670693
1106	18-19	-0.09623673358662188
1106	20-21	-0.07392583848650247
1106	22-23	-0.10244772532264079
1106	24-25	-0.10999985943698931
1106	26-27	-0.13902696412962712
1106	28-29	-0.18552665306263094
1106	30-31	-0.22771031232534966
1106	32-33	-0.18519692833651646
1106	34-35	-0.10534197017395996
1106	36-37	-0.1640253078786813
1106	38-39	-0.10373778300544956
1106	40-41	-0.10495285346546979
1106	42-43	-0.08909304638770266
1106	44-45	-0.07914577533801292
1106	46-47	-0.11619212908284737
1106	48-49	-0.11934742085429306
1106	50-51	0.00974978546459937
1106	52-53	0.007960479446790458
1106	54-55	-0.0326295444673903
1106	56-57	0.025622311941607023
1106	58-59	0.05388128577817497
1106	60-61	0.05881844288201421
1106	62-63	-0.00993661275330382
1106	64-65	0.06599282559368191
1106	66-67	0.0501195077595078
1106	68-69	0.08180355091934643
1106	70-71	0.1576884253353974
1106	72-73	0.14034211576229794
1106	74-75	0.22648894506151507
1106	76-77	0.2586707929206966
1106	78-79	0.29497863849861616
1106	80-81	0.3114599926286168
1106	82-83	0.3710784894645549
1106	84-85	0.36500280608562363
1106	86-87	0.31328867502236335
1106	88-89	0.32127428882557396
1106	90-91	0.3590006058406239
1106	92-93	0.24601383063153648
1106	94-95	0.28117695283984645
1106	96-97	0.16118314019195523
1106	98-99	0.18640112323471314
1106	100	0.18183430836542414
1107	1	-0.23897239031941453
1107	2	-0.2447556534461377
1107	3	-0.22818930143955996
1107	4	-0.18066311251336487
1107	5	-0.22219542768986145
1107	6	-0.3233757426598416
1107	7	-0.2708458523247401
1107	8	-0.22425966302321854
1107	9	-0.2652124440748054
1107	10-11	-0.23007394501331646
1107	12-13	-0.176500831250884
1107	14-15	-0.21246252997262616
1107	16-17	-0.19356938988959627
1107	18-19	-0.15113310955505455
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2208	3	-0.617332541809386
2208	4	-0.5014822958469267
2208	5	-0.742140066829144
2208	6	-0.6323418276036676
2208	7	-0.6248351988630247
2208	8	-0.5882220782807153
2208	9	-0.7114623951235899
2208	10-11	-0.6715259235091438
2208	12-13	-0.6705234287815003
2208	14-15	-0.7215443667620747
2208	16-17	-0.707700839004751
2208	18-19	-0.7026150382296024
2208	20-21	-0.6961401259847833
2208	22-23	-0.6631395075223381
2208	24-25	-0.8000995930879427
2208	26-27	-0.8091285935418782
2208	28-29	-0.9119805430782293
2208	30-31	-1.0195297014546796
2208	32-33	-0.991460639591125
2208	34-35	-0.913278718971057
2208	36-37	-0.9423770965440283
2208	38-39	-0.9239836967688078
2208	40-41	-0.8704310135867956
2208	42-43	-0.8834442497194317
2208	44-45	-0.9060377637378565
2208	46-47	-0.8906132843205015
2208	48-49	-0.8243799588191756
2208	50-51	-0.7599731658656026
2208	52-53	-0.6754744255197416
2208	54-55	-0.6055363283126312
2208	56-57	-0.6211564464919839
2208	58-59	-1.1153112712903699
2208	60-61	-0.7490308237930137
2208	62-63	-0.6902216037407172
2208	64-65	-0.60643740115054
2208	66-67	-0.5205792658025317
2208	68-69	-0.510157869672863
2208	70-71	-0.4072675948099551
2208	72-73	-0.3461069048763683
2208	74-75	-0.35492382377675114
2208	76-77	-0.3306512727706732
2208	78-79	-0.29439895770996216
2208	80-81	-0.2280110857865978
2208	82-83	-0.1211140262043564
2208	84-85	-0.10440579966426711
2208	86-87	-0.08619948581085168
2208	88-89	-0.014532505688880093
2208	90-91	-0.8575361429587325
2208	92-93	-0.45235486943071734
2208	94-95	-0.16029447354843995
2208	96-97	0.24565467574083755
2208	98-99	0.3715175145164231
2208	100	0.3190156971395677
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	457478.0
3	153134.0
4	137409.0
5	124755.0
6	116684.0
7	137855.0
8	168048.0
9	184278.0
10	192744.0
11	203131.0
12	233004.0
13	299345.0
14	410936.0
15	512947.0
16	600442.0
17	713579.0
18	1067120.0
19	850138.0
20	562797.0
21	482297.0
22	481989.0
23	508603.0
24	548679.0
25	598317.0
26	655706.0
27	710060.0
28	767946.0
29	828002.0
30	913871.0
31	963029.0
32	971394.0
33	1101204.0
34	1294088.0
35	1578899.0
36	2108786.0
37	2964037.0
38	3014777.0
39	653249.0
40	695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.029218730240924	38.257235190840646	23.954338740536148	19.75920733838228
2	20.195866673038157	23.346086931897624	37.52260197480784	18.93544442025638
3	21.1013705942026	36.73935419747571	21.866750578189563	20.29252463013212
4	21.552383846625844	20.812647018048093	36.84708267903472	20.787886456291343
5	30.515425395549585	21.119453643125617	21.38153123229645	26.983589729028353
6	30.48618230138705	21.212244738076578	27.231165613979442	21.070407346556934
7	22.51252820467111	29.81050487789939	20.79353428824975	26.883432629179755
8	28.877680540633143	21.350884422108418	28.98659378515877	20.784841252099664
9	21.930845398031174	27.91231252747231	20.93842756089343	29.218414513603086
10-11	32.27867478378239	21.07563300787486	21.210222388455342	25.43546981988741
12-13	28.329122402684725	21.20852910576972	21.54566774146428	28.916680750081277
14-15	27.58265784879159	21.689595017097414	22.80461110966635	27.923136024444638
16-17	26.854786865157664	21.625521045413013	23.512970300422513	28.00672178900681
18-19	26.106958053388713	21.630182910685974	25.33708657400009	26.925772461925224
20-21	21.67891914433286	24.727815466020804	30.922898819466027	22.670366570180313
22-23	21.454044448891484	24.6769819905994	31.69909993862406	22.16987362188506
24-25	17.426365242620115	20.789144842318485	36.5894159558965	25.195073959164898
26-27	17.424906153919743	20.806443007840812	44.422506968936474	17.34614386930297
28-29	17.398318324165892	20.860937250469778	44.43963962714555	17.301104798218784
30-31	17.381116393132746	20.844288965401176	44.421818046994645	17.35277659447144
32-33	17.410107547302246	20.910204554691507	44.31856165283043	17.36112624517581
34-35	17.461568974460683	20.979827420680774	44.26082445915846	17.297779145700087
36-37	17.51204155088839	21.040736776582463	44.04803215450658	17.399189518022563
38-39	17.598906080087765	21.19023865619571	43.71911153007268	17.491743733643847
40-41	17.71711195837491	21.417159651679842	43.25791602104279	17.60781236890245
42-43	17.90168701284073	21.68509406182866	42.52397584645509	17.889243078875523
44-45	18.10446099039628	22.132468073174984	41.670965481228194	18.092105455200542
46-47	18.445212648395515	22.69271220209022	40.51064897433639	18.351426175177878
48-49	19.008913457691108	23.731566749636556	38.25051788219837	19.009001910473966
50-51	19.653491319981732	25.376812966879957	35.273948962955224	19.695746750183087
52-53	20.084767067822728	26.593434540576684	33.257449748641264	20.064348642959324
54-55	20.384691543397796	27.176174405133125	31.99211437283579	20.44701967863329
56-57	20.5959639711484	27.636467461550644	31.16214035783843	20.605428209462524
58-59	20.732586305090734	27.975840100747206	30.69875082777304	20.59282276638902
60-61	20.760846890527805	28.293970305478755	30.301728283835335	20.643454520158112
62-63	20.947877485070933	28.769921283418043	29.760406907747605	20.52179432376342
64-65	21.048030515131742	29.44331044976724	29.04548277669531	20.463176258405706
66-67	21.007504161977764	30.121379853153485	28.402415163085415	20.46870082178334
68-69	21.034652666151157	30.75361262539682	27.73223094320215	20.479503765249866
70-71	21.159905472506217	31.330800519718665	27.142626022684023	20.366667985091098
72-73	21.23341791218019	31.83077372150806	26.446806476067742	20.48900189024401
74-75	21.377174319285057	32.29938620763544	25.926683652785464	20.396755820294025
76-77	21.24220849952409	32.85537509363722	25.363760497376724	20.538655909461962
78-79	21.34847851900721	33.079383465909515	24.977366810712457	20.59477120437082
80-81	21.44075238527511	33.322682918878385	24.575037762128122	20.66152693371838
82-83	21.54094268461761	33.50684162527819	24.144320583763598	20.807895106340595
84-85	21.64680258564276	33.49638869866058	23.85427116878486	21.002537546911807
86-87	21.80648689150953	33.52325802864007	23.491919454456664	21.178335625393736
88-89	21.9974035162775	33.40930823210373	23.176090300798755	21.417197950820004
90-91	22.131636044606513	33.22960922915429	22.831683081438257	21.807071644800942
92-93	22.395431989856263	33.01906503613603	22.684888715926142	21.90061425808157
94-95	22.57305609504584	32.817823432813434	22.389038305118785	22.220082167021943
96-97	22.71430317762286	32.61775181043549	22.096593637263204	22.571351374678446
98-99	23.197712394038298	32.21283203548817	21.89059292609892	22.698862644374614
100	24.307527996787584	31.705629204898596	20.948670998595983	23.038171799717837
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3149.0
1	2501.5
2	1963.0
3	2015.0
4	2008.5
5	2024.0
6	2177.5
7	2417.0
8	2829.5
9	3595.0
10	5039.0
11	7643.0
12	10190.0
13	16739.0
14	34228.5
15	57517.0
16	234960.5
17	342193.5
18	281210.0
19	284030.0
20	292907.5
21	293085.0
22	283435.5
23	270767.5
24	262531.5
25	266766.5
26	280633.5
27	299644.5
28	322953.5
29	349281.0
30	374574.0
31	401508.0
32	435721.5
33	473514.0
34	510425.0
35	558065.0
36	618416.0
37	690025.5
38	761000.0
39	808833.0
40	847525.5
41	895928.0
42	967670.0
43	1034097.0
44	1076712.5
45	1095374.0
46	1079475.0
47	1039567.0
48	990561.5
49	959172.5
50	920664.5
51	857095.5
52	782356.5
53	702909.0
54	637645.5
55	581825.0
56	519565.5
57	459714.0
58	410322.0
59	365714.5
60	323970.0
61	283445.0
62	245805.5
63	212530.0
64	184392.0
65	160033.0
66	137484.0
67	117077.5
68	98951.5
69	82852.5
70	68517.0
71	56111.0
72	45804.5
73	37423.5
74	30488.0
75	24802.5
76	19907.5
77	15773.5
78	12541.0
79	9818.5
80	7626.5
81	5869.5
82	4396.0
83	3234.5
84	2338.5
85	1674.5
86	1180.5
87	782.5
88	556.0
89	409.5
90	281.5
91	202.0
92	130.0
93	78.5
94	51.0
95	30.5
96	21.5
97	12.5
98	4.5
99	2.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1721347739762358
2	0.07287209726617508
3	0.06887513241272504
4	0.08814545499820808
5	0.09466439856007396
6	0.03862907359692739
7	0.03756085821131507
8	0.030384007160297252
9	0.03953811781580939
10-11	0.03514322504553356
12-13	0.02936354312470403
14-15	0.02973671108226065
16-17	0.02985520517304877
18-19	0.03163084796635136
20-21	0.030889817756795796
22-23	0.028067182400111607
24-25	0.029674811184087747
26-27	0.021293564971477233
28-29	0.020649806030479088
30-31	0.01962757342636664
32-33	0.01968063048194341
34-35	0.016764260993740256
36-37	0.020085632672846094
38-39	0.024264760083776385
40-41	0.022915342303607186
42-43	0.030721803747469353
44-45	0.031181631562468032
46-47	0.03129481994769848
48-49	0.0275366118443439
50-51	0.026984818466345487
52-53	0.027460563398017192
54-55	0.028765766965205747
56-57	0.02532413262679257
58-59	0.020706400223094307
60-61	0.01062909680054636
62-63	0.008630614373821337
64-65	0.0059211674023675895
66-67	0.007567704693766701
68-69	0.012523233684637067
70-71	0.019065168637252872
72-73	0.01673419532891342
74-75	0.01643177011212583
76-77	0.02163489869568779
78-79	0.01924202548917544
80-81	0.011960828895523301
82-83	0.014569467461381184
84-85	0.017613173882968586
86-87	0.021670270066072306
88-89	0.025018170272966524
90-91	0.030044442004605917
92-93	0.0844632953411802
94-95	0.13263202753081094
96-97	0.08672706304578909
98-99	0.06918109476655109
100	0.07452040312609341
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.8271452E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.368759707524234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.67139761325654	32.16235505812173
2	10.434249256345534	8.424354019156526
3	3.6267478019448243	4.392219316095068
4	1.8582561332563456	3.000619812738342
5	1.0290299876159315	2.0770332150952084
6	0.6777886988238345	1.6416893469176932
7	0.4478871590418697	1.265645436961285
8	0.32581875677470185	1.0522319280353778
9	0.2460144153193811	0.8938167134953646
>10	1.3777586381582267	10.00437104562562
>50	0.1198853320367528	3.4598204653216946
>100	0.17277809908532973	14.188309495882635
>500	0.010307243302779302	2.6104367762801126
>1k	0.0015332917388172801	1.1600944669207465
>5k	1.9614565935456948E-4	0.5614897090981035
>10k+	3.514276396709047E-4	13.105513194254526
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	1354811	4.792152168201336	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	1114275	3.941343373520398	No Hit
ATATGGATCCGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	112129	0.39661563898451346	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99444	0.3517470556517578	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA	76814	0.27170164447160333	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66441	0.23501092197174733	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	64788	0.22916403444718722	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	64164	0.22695686093519357	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	55241	0.195394987141092	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC	49242	0.17417570204742225	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA	48862	0.17283158997281073	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45071	0.1594223034600416	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44379	0.15697460462943327	No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43607	0.15424393483574877	No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43435	0.15363554726513515	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG	43331	0.15326768501313623	No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT	40220	0.14226365168651403	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37602	0.13300342691984832	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	37547	0.1328088843827335	No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34308	0.12135209751518952	No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33858	0.11976038584788641	No Hit
TCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32385	0.11455018299024755	No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31871	0.11273209455248355	No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30213	0.10686752134273117	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG	29112	0.10297313346339622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.5371370384513676E-6	0.0
3	0.0	0.0	0.0	3.5371370384513676E-6	0.0
4	0.0	0.0	0.0	7.074274076902735E-6	0.0
5	0.0	0.0	0.0	7.074274076902735E-6	0.0
6	0.0	0.0	0.0	7.074274076902735E-6	0.0
7	3.5371370384513676E-6	0.0	0.0	7.074274076902735E-6	0.0
8	3.5371370384513676E-6	7.074274076902735E-6	0.0	7.074274076902735E-6	0.0
9	3.5371370384513676E-6	7.074274076902735E-6	0.0	7.074274076902735E-6	0.0
10-11	5.305705557677052E-6	7.074274076902735E-6	0.0	1.2379979634579787E-5	0.0
12-13	1.414854815380547E-5	8.84284259612842E-6	0.0	1.768568519225684E-5	0.0
14-15	2.299139074993389E-5	1.0611411115354103E-5	0.0	1.945425371148252E-5	1.7685685192256838E-6
16-17	8.312272040360714E-5	1.5917116673031154E-5	0.0	2.4759959269159574E-5	3.5371370384513676E-6
18-19	1.892368315571482E-4	1.768568519225684E-5	0.0	3.183423334606231E-5	3.5371370384513676E-6
20-21	4.103078964603587E-4	1.945425371148252E-5	0.0	3.360280186528799E-5	3.5371370384513676E-6
22-23	9.567955689010949E-4	2.1222822230708207E-5	0.0	3.713993890373937E-5	3.5371370384513676E-6
24-25	0.0017155114636489134	2.1222822230708207E-5	0.0	4.067707594219073E-5	3.5371370384513676E-6
26-27	0.002656389915876977	2.1222822230708207E-5	0.0	5.1288487057544836E-5	3.5371370384513676E-6
28-29	0.00540474539475369	2.1222822230708207E-5	0.0	5.659419261522188E-5	3.5371370384513676E-6
30-31	0.007580084673401282	2.1222822230708207E-5	0.0	5.659419261522188E-5	3.5371370384513676E-6
32-33	0.00794971549391945	2.2991390749933892E-5	0.0	6.189989817289893E-5	3.5371370384513676E-6
34-35	0.008386551918168193	2.4759959269159574E-5	0.0	6.7205603730576E-5	3.5371370384513676E-6
36-37	0.009044459407320148	2.4759959269159574E-5	3.5371370384513676E-6	6.7205603730576E-5	3.5371370384513676E-6
38-39	0.010045469189201885	2.829709630761094E-5	3.5371370384513676E-6	6.7205603730576E-5	5.305705557677052E-6
40-41	0.011271087173025284	3.183423334606231E-5	3.5371370384513676E-6	6.7205603730576E-5	7.074274076902735E-6
42-43	0.012899938779232139	3.183423334606231E-5	3.5371370384513676E-6	7.427987780747874E-5	7.074274076902735E-6
44-45	0.015158400778283338	3.537137038451368E-5	5.305705557677052E-6	8.312272040360714E-5	8.84284259612842E-6
46-47	0.018518680964812136	3.537137038451368E-5	7.074274076902735E-6	8.842842596128419E-5	1.0611411115354103E-5
48-49	0.022986085044376214	3.890850742296504E-5	7.074274076902735E-6	8.842842596128419E-5	1.0611411115354103E-5
50-51	0.02942898015991538	4.7751350019093466E-5	7.074274076902735E-6	8.842842596128419E-5	1.0611411115354103E-5
52-53	0.037960554696660084	5.305705557677052E-5	8.84284259612842E-6	9.550270003818693E-5	1.0611411115354103E-5
54-55	0.04897873657143609	5.659419261522188E-5	1.0611411115354103E-5	9.90398370766383E-5	1.2379979634579787E-5
56-57	0.06363309532174011	5.8362761134447564E-5	1.0611411115354103E-5	1.0257697411508967E-4	1.768568519225684E-5
58-59	0.08220837047916746	6.013132965367325E-5	1.0611411115354103E-5	1.0257697411508967E-4	1.768568519225684E-5
60-61	0.10582760305342648	6.013132965367325E-5	1.0611411115354103E-5	1.0611411115354104E-4	1.768568519225684E-5
62-63	0.1351769976299767	6.013132965367325E-5	1.0611411115354103E-5	1.0611411115354104E-4	1.768568519225684E-5
64-65	0.17290586985061823	6.013132965367325E-5	1.0611411115354103E-5	1.0611411115354104E-4	1.768568519225684E-5
66-67	0.2201496407046939	6.013132965367325E-5	1.0611411115354103E-5	1.0788267967276673E-4	1.768568519225684E-5
68-69	0.2768216503347617	6.189989817289893E-5	1.0611411115354103E-5	1.1141981671121808E-4	2.1222822230708207E-5
70-71	0.34635999594219635	6.7205603730576E-5	1.0611411115354103E-5	1.1495695374966945E-4	2.1222822230708207E-5
72-73	0.4290529541956317	7.074274076902736E-5	1.0611411115354103E-5	1.1849409078812083E-4	2.2991390749933892E-5
74-75	0.5288709614207292	7.604844632670441E-5	1.0611411115354103E-5	1.202626593073465E-4	2.6528527788385256E-5
76-77	0.6462455483361802	8.312272040360714E-5	1.0611411115354103E-5	1.2379979634579788E-4	3.183423334606231E-5
78-79	0.7810759065363888	8.842842596128419E-5	1.0611411115354103E-5	1.2733693338424923E-4	3.183423334606231E-5
80-81	0.9343931114680633	9.373413151896124E-5	1.0611411115354103E-5	1.326426389419263E-4	3.183423334606231E-5
82-83	1.1097219201900206	9.90398370766383E-5	1.0611411115354103E-5	1.34411207461152E-4	3.183423334606231E-5
84-85	1.3045668825216334	9.90398370766383E-5	1.0611411115354103E-5	1.34411207461152E-4	3.183423334606231E-5
86-87	1.5208415896007037	1.0434554263431535E-4	1.0611411115354103E-5	1.39716913018829E-4	3.183423334606231E-5
88	1.695211834185241	1.0611411115354104E-4	1.0611411115354103E-5	1.4148548153805472E-4	3.183423334606231E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	202630	0.0	89.41631	1
TATCTCG	204590	0.0	87.88822	2
TATGGAT	251490	0.0	86.66015	2
ATATGGA	253275	0.0	86.375374	1
ATGGATC	254505	0.0	85.36853	3
TGGATCC	261645	0.0	82.694725	4
GGATCCG	261180	0.0	82.641975	5
ATCTCGA	222675	0.0	80.17926	3
TCTCGAG	229885	0.0	77.39779	4
GATCCGG	281315	0.0	76.728226	6
CTCGAGG	241740	0.0	73.5198	5
ATCCGGC	293120	0.0	72.953094	7
TCGAGGG	252295	0.0	70.33423	6
TCCGGCG	309230	0.0	69.1116	8
CCGGCGC	326140	0.0	65.52536	9
CGAGGGC	271115	0.0	65.19586	7
GAGGGCG	290725	0.0	60.814312	8
AGGGCGC	291655	0.0	60.478596	9
CCGTCGA	289990	0.0	33.359398	16-17
GTCGACT	290035	0.0	33.206573	18-19
>>END_MODULE