FastQCFastQC Report
Wed 25 May 2016
SRR490978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32482770
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT13831064.257968147420925No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT10916043.360563153942844No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2610120.8035398458936847No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA885100.27248291940619596No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA769660.23694407835292372No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT685610.21106882202472266No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA609540.1876502527339879No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG602540.18549526410463146No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT592020.18225662405022724No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC530480.16331119544299946No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA487000.1499256374995113No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG476870.14680706109731406No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381970.11759157239361051No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT331380.10201716171373315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC2029950.088.3162841
TATCTCG2023200.088.042372
TATGGAT2454800.086.551312
ATGGATC2480500.085.434393
ATATGGA2506200.085.150431
GGATCCG2505150.084.035175
TGGATCC2539500.083.094524
ATCTCGA2165250.081.723183
TCTCGAG2223700.079.267234
GATCCGG2669750.078.803886
CTCGAGG2309050.076.308265
ATCCGGC2756250.075.801437
TCGAGGG2399700.073.3453456
TCCGGCG2875500.072.743978
CCGGCGC3016850.069.455619
CGAGGGC2577150.068.180477
AGGGCGC2701800.064.957639
GAGGGCG2741450.064.1518558
CCGTCGA2668800.036.07324216-17
GTCGACT2672100.035.85991718-19
Produced by FastQC (version 0.11.5)