FastQCFastQC Report
Wed 25 May 2016
SRR490977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27602538
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT7993312.895860518333495No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT6632372.4028116544935108No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT684080.2478322826690792No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA488590.17700908517905128No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470400.1704191114599679No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG407670.14769294041004488No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC389390.1410703609936159No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA387880.14052330984926095No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG368970.13367249055141234No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA344740.1248943122549093No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA312940.11337363252611048No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312720.11329392971037663No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293420.1063018190573635No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC284660.10312819784905286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC1305400.085.528781
TATCTCG1301000.084.97392
TATGGAT1545650.083.238252
ATGGATC1582050.081.188293
ATATGGA1598000.080.859491
GGATCCG1615650.078.986365
TGGATCC1645300.077.8528064
ATCTCGA1448050.075.76573
TCTCGAG1511800.072.359274
GATCCGG1779600.071.6354456
CTCGAGG1608900.067.916075
ATCCGGC1863750.067.4960257
TCGAGGG1703600.063.98936
TCCGGCG1993400.063.05368
CCGGCGC2137250.058.7613339
CGAGGGC1876250.057.8364947
AGGGCGC2009500.053.8016939
GAGGGCG2034050.053.2932748
CCGTCGA1918400.030.45962316-17
GTCGACT1919700.030.28964418-19
Produced by FastQC (version 0.11.5)