##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490977_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27602538
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.957121877705593	31.0	31.0	34.0	28.0	34.0
2	31.21121376592254	33.0	31.0	34.0	27.0	34.0
3	31.33665715812075	34.0	31.0	34.0	28.0	34.0
4	34.68455096411787	37.0	35.0	37.0	32.0	37.0
5	34.5071330759512	37.0	35.0	37.0	30.0	37.0
6	34.400500453979994	37.0	35.0	37.0	30.0	37.0
7	34.386011061736426	37.0	35.0	37.0	30.0	37.0
8	34.276028457962816	37.0	35.0	37.0	30.0	37.0
9	35.7473801865611	39.0	37.0	39.0	30.0	39.0
10-11	35.63729065421448	39.0	36.0	39.0	30.0	39.0
12-13	35.451611007654435	39.0	35.0	39.0	30.0	39.0
14-15	36.45238763913666	40.0	36.5	41.0	28.5	41.0
16-17	36.326546022688206	40.0	36.0	41.0	28.0	41.0
18-19	36.252341560040605	40.0	36.0	41.0	27.5	41.0
20-21	35.923720003573585	39.5	36.0	41.0	27.0	41.0
22-23	35.748356491711014	39.5	36.0	41.0	26.5	41.0
24-25	35.71387747025292	39.0	36.0	41.0	26.5	41.0
26-27	35.666762817245285	39.0	35.0	41.0	27.0	41.0
28-29	35.68607317921273	39.0	35.0	41.0	27.0	41.0
30-31	35.448904336260675	39.0	35.0	41.0	25.5	41.0
32-33	34.796098587021234	38.0	35.0	41.0	24.5	41.0
34-35	34.446234255705036	38.0	35.0	41.0	23.5	41.0
36-37	34.13304742121902	38.0	34.0	40.0	20.5	41.0
38-39	33.807348711919175	38.0	34.0	40.0	17.0	41.0
40-41	33.41241722047444	38.0	33.0	40.0	12.0	41.0
42-43	32.9590481317334	38.0	33.0	40.0	6.5	41.0
44-45	32.474269141482566	38.0	33.0	40.0	2.0	41.0
46-47	31.810672917106395	37.5	31.5	40.0	2.0	41.0
48-49	30.816122742046403	37.0	29.5	40.0	2.0	41.0
50-51	29.886124384648976	36.5	26.5	40.0	2.0	41.0
52-53	29.51460139281395	36.0	25.5	40.0	2.0	41.0
54-55	29.19065877203031	36.0	24.0	40.0	2.0	41.0
56-57	28.736497654672192	35.5	22.5	40.0	2.0	41.0
58-59	28.317325421307274	35.0	21.0	40.0	2.0	41.0
60-61	27.872225372898683	35.0	19.0	39.0	2.0	41.0
62-63	27.3940973109067	35.0	16.0	39.0	2.0	41.0
64-65	26.8519587582852	34.0	9.5	39.0	2.0	40.5
66-67	26.308455820258267	34.0	6.5	38.0	2.0	40.0
68-69	25.70487925784216	33.5	2.0	37.0	2.0	40.0
70-71	25.108124694910302	33.0	2.0	36.5	2.0	39.5
72-73	24.496587831887055	32.5	2.0	36.0	2.0	39.0
74-75	23.86000953970247	32.0	2.0	35.5	2.0	39.0
76-77	23.213984833568567	31.0	2.0	35.0	2.0	37.5
78-79	22.848899836674438	31.0	2.0	35.0	2.0	37.0
80-81	22.26421014256008	31.0	2.0	35.0	2.0	36.5
82-83	21.61522590784949	30.0	2.0	35.0	2.0	36.0
84-85	21.062986236990234	29.5	2.0	35.0	2.0	36.0
86-87	20.439651219753777	29.0	2.0	34.0	2.0	35.0
88-89	19.7579552829526	28.0	2.0	34.0	2.0	35.0
90-91	19.04828099503024	25.5	2.0	34.0	2.0	35.0
92-93	18.387787999784656	24.5	2.0	34.0	2.0	35.0
94-95	17.61424179544649	21.5	2.0	34.0	2.0	35.0
96-97	16.8649754779796	10.0	2.0	33.5	2.0	35.0
98-99	16.14066766251712	2.0	2.0	33.0	2.0	35.0
100	15.218834876705904	2.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1006977450383637
1101	2	0.1233802693577104
1101	3	0.17698683315405717
1101	4	0.208713914499576
1101	5	0.174108161562053
1101	6	0.21134169540175662
1101	7	0.21948642448559497
1101	8	0.2182797503886036
1101	9	0.23502447430328743
1101	10-11	0.2210565256039061
1101	12-13	0.18349156919107656
1101	14-15	0.1886619484204317
1101	16-17	0.13412572015455737
1101	18-19	0.12962633697597425
1101	20-21	0.04413494387139849
1101	22-23	0.05960299304943817
1101	24-25	0.046305074856697104
1101	26-27	0.13952549626334587
1101	28-29	0.21295506626261584
1101	30-31	0.2284115062618568
1101	32-33	0.26396339325928153
1101	34-35	0.21918222969080148
1101	36-37	0.21995286246677637
1101	38-39	0.11615975372782827
1101	40-41	0.12959901761893633
1101	42-43	0.12439619127133739
1101	44-45	0.07632955930690599
1101	46-47	0.07797508712632961
1101	48-49	0.07650265343666973
1101	50-51	0.019901607595766535
1101	52-53	-0.05522213081919247
1101	54-55	-0.10137074543104774
1101	56-57	-0.12329525402503805
1101	58-59	-0.2146226382344203
1101	60-61	-0.21813529401214637
1101	62-63	-0.3497921551902863
1101	64-65	-0.3256809392778237
1101	66-67	-0.3804617031461568
1101	68-69	-0.4319361393469521
1101	70-71	-0.47202592806592136
1101	72-73	-0.5851724254122423
1101	74-75	-0.6224026536235776
1101	76-77	-0.7094394444387113
1101	78-79	-0.8047945317106233
1101	80-81	-0.788762196840807
1101	82-83	-0.8081174357004848
1101	84-85	-0.957915853541401
1101	86-87	-1.08329368083119
1101	88-89	-1.2111248625690791
1101	90-91	-1.297371546167362
1101	92-93	-1.3843360178303463
1101	94-95	-1.4595743708021764
1101	96-97	-1.6283288905762916
1101	98-99	-1.6380316772792742
1101	100	-1.5199610916036512
1102	1	0.11976867563530291
1102	2	0.17278036580027845
1102	3	0.17965329045973633
1102	4	0.2050560803290935
1102	5	0.2086183885218844
1102	6	0.23459163020536522
1102	7	0.23105176194736288
1102	8	0.2594326156164328
1102	9	0.25177052508511366
1102	10-11	0.26544743747644617
1102	12-13	0.2107929572113747
1102	14-15	0.2636317141399971
1102	16-17	0.23643363290855746
1102	18-19	0.263101885916754
1102	20-21	0.22263667583548852
1102	22-23	0.20154298283381422
1102	24-25	0.2076241300290178
1102	26-27	0.25099724580739036
1102	28-29	0.2840742980199096
1102	30-31	0.2596509876019937
1102	32-33	0.2584678889061891
1102	34-35	0.2839737277490002
1102	36-37	0.27522109459010835
1102	38-39	0.2230214811930864
1102	40-41	0.2187736773847675
1102	42-43	0.18516677895771494
1102	44-45	0.13126408292907144
1102	46-47	0.15192531658064823
1102	48-49	0.17609267366260895
1102	50-51	0.23320126315747913
1102	52-53	0.160121399581179
1102	54-55	0.06587528481606952
1102	56-57	0.07805052899313125
1102	58-59	-0.009538852678684151
1102	60-61	-0.04609787356590189
1102	62-63	-0.11817935968998938
1102	64-65	-0.10969711885937983
1102	66-67	-0.146725420151256
1102	68-69	-0.19893255623199124
1102	70-71	-0.2134135163144073
1102	72-73	-0.2599283313769156
1102	74-75	-0.27430835398479303
1102	76-77	-0.34343895563771554
1102	78-79	-0.37128823258456123
1102	80-81	-0.41127948513602774
1102	82-83	-0.4216932018241195
1102	84-85	-0.526314978093545
1102	86-87	-0.6058016585388657
1102	88-89	-0.6571899552482598
1102	90-91	-0.7255949107208224
1102	92-93	-0.8129339891060745
1102	94-95	-0.8443171634799995
1102	96-97	-0.9469015637089306
1102	98-99	-1.0060488832864731
1102	100	-0.9471090033184595
1103	1	0.027614161468275
1103	2	0.019473859474413047
1103	3	0.011363388664847207
1103	4	0.02654240356858395
1103	5	0.06880660724505105
1103	6	0.09832773639404024
1103	7	0.07072012573370756
1103	8	0.10984848143441184
1103	9	0.06368720141055206
1103	10-11	0.07629667171337218
1103	12-13	0.05312834273684075
1103	14-15	0.09789203891806864
1103	16-17	0.07312874944258851
1103	18-19	0.07991846150599713
1103	20-21	0.08142804793195069
1103	22-23	0.11784891634501093
1103	24-25	0.10048610422714432
1103	26-27	0.07418375281018541
1103	28-29	0.13960999099600002
1103	30-31	0.06559618265628586
1103	32-33	0.0510240063631926
1103	34-35	0.07610798273074693
1103	36-37	0.032898492036487426
1103	38-39	-0.008243687772861108
1103	40-41	0.04023229488651481
1103	42-43	0.05142169836183541
1103	44-45	-0.001464853867375382
1103	46-47	-0.05309038259276022
1103	48-49	-0.1654657298500375
1103	50-51	-0.08090805308745885
1103	52-53	-0.02641791803982585
1103	54-55	-0.06113124150856564
1103	56-57	-0.04241548800078476
1103	58-59	-0.11488381739678033
1103	60-61	-0.1546036978543306
1103	62-63	-0.23347084856164457
1103	64-65	-0.27208316298843016
1103	66-67	-0.2569664929370781
1103	68-69	-0.2654295775370379
1103	70-71	-0.2963435321421173
1103	72-73	-0.3685123393226455
1103	74-75	-0.3751498238764768
1103	76-77	-0.36161591297216944
1103	78-79	-0.41799629752349077
1103	80-81	-0.4113929411541264
1103	82-83	-0.37093633613589105
1103	84-85	-0.39125965944181473
1103	86-87	-0.5116194784758825
1103	88-89	-0.4747859075033638
1103	90-91	-0.49765299394429974
1103	92-93	-0.5332283024931499
1103	94-95	-0.6241298196724934
1103	96-97	-0.7240926421291718
1103	98-99	-0.6945053044933864
1103	100	-0.6822462188039893
1104	1	-0.05381659452547538
1104	2	-0.006740475387477574
1104	3	-0.03470192832306296
1104	4	0.040632512574724444
1104	5	-0.06333238965615351
1104	6	-0.029641059242550227
1104	7	-0.013223730756536156
1104	8	-0.04835697920896109
1104	9	-0.0502254111337308
1104	10-11	-0.05378943450747897
1104	12-13	-0.022513542988590984
1104	14-15	0.06785429818046396
1104	16-17	0.03355773115690397
1104	18-19	0.08076646314110292
1104	20-21	0.07471584854719993
1104	22-23	0.05732573768395355
1104	24-25	-0.05628509171024376
1104	26-27	0.04383657730095791
1104	28-29	0.08068797634542335
1104	30-31	0.02932979125233004
1104	32-33	0.08240050542541866
1104	34-35	0.10409074372445559
1104	36-37	0.08780450210593216
1104	38-39	0.12336789862302311
1104	40-41	0.08963559754712236
1104	42-43	0.044679798792238046
1104	44-45	0.09382126861734719
1104	46-47	0.013392843002517196
1104	48-49	0.11484916809168055
1104	50-51	-0.010211366309437864
1104	52-53	0.01421294412633145
1104	54-55	0.0973673790576619
1104	56-57	0.04977831898694873
1104	58-59	0.024442995601066997
1104	60-61	0.023924553474401478
1104	62-63	-0.03373239171484599
1104	64-65	0.039351471900562984
1104	66-67	-0.023204705262958925
1104	68-69	0.012569614036650023
1104	70-71	0.12146378024462834
1104	72-73	0.057946630564583046
1104	74-75	0.16701233027534457
1104	76-77	0.1556177539066823
1104	78-79	0.079471169561252
1104	80-81	0.1083872119750211
1104	82-83	0.05057295647211646
1104	84-85	0.11484353650296342
1104	86-87	0.05697832236604938
1104	88-89	0.11489898844213187
1104	90-91	0.047528422621461175
1104	92-93	0.0298367369721646
1104	94-95	0.027147760055871828
1104	96-97	-0.09792871671387005
1104	98-99	-0.11638850368732534
1104	100	-0.048659672651174546
1105	1	-0.02144299981592468
1105	2	-0.010202940625344326
1105	3	-0.019395818957161026
1105	4	0.00783347746450147
1105	5	-0.03591109054696773
1105	6	0.03889937884075323
1105	7	-0.008547648079769488
1105	8	-0.0023337982441802296
1105	9	0.018769625649007082
1105	10-11	0.022313736981963928
1105	12-13	0.06611029423987702
1105	14-15	0.043991348699584876
1105	16-17	0.013574340363916804
1105	18-19	0.020532949183021287
1105	20-21	0.01625526808362565
1105	22-23	0.0020845882074809197
1105	24-25	0.0058126360194137305
1105	26-27	0.035412126129898525
1105	28-29	-0.05647072021685773
1105	30-31	0.016923118200146803
1105	32-33	0.039799548822657016
1105	34-35	-0.04680235263330701
1105	36-37	-0.04737075258616841
1105	38-39	0.013500142935114923
1105	40-41	0.09769430217490793
1105	42-43	0.09953893129571867
1105	44-45	0.14303403820098737
1105	46-47	0.09713443334549154
1105	48-49	0.14639407321385178
1105	50-51	0.2629040685261721
1105	52-53	0.20066512916804058
1105	54-55	0.2235034080330216
1105	56-57	0.08008577043202436
1105	58-59	0.21373963405757834
1105	60-61	0.2066860703053095
1105	62-63	0.21371150318654486
1105	64-65	0.2090614928930279
1105	66-67	0.26973190924981694
1105	68-69	0.3108602084461225
1105	70-71	0.3211612540998168
1105	72-73	0.22264935937637986
1105	74-75	0.22198345081633164
1105	76-77	0.29858421339368135
1105	78-79	0.30421360046273804
1105	80-81	0.33272570701434745
1105	82-83	0.35210873936487985
1105	84-85	0.42503146993427166
1105	86-87	0.40553795348451516
1105	88-89	0.45615118739976523
1105	90-91	0.4487249005366323
1105	92-93	0.410186406579637
1105	94-95	0.4067629507763968
1105	96-97	0.34257255441752577
1105	98-99	0.37958073807312687
1105	100	0.38378862374870515
1106	1	-0.22660364110737063
1106	2	-0.19187361980876005
1106	3	-0.17297004588706244
1106	4	-0.09188918160396753
1106	5	-0.12764689573641164
1106	6	-0.09256131369013332
1106	7	-0.16207119214300292
1106	8	-0.14904663508145433
1106	9	-0.20680904684308388
1106	10-11	-0.1584158878283901
1106	12-13	-0.12120709967667409
1106	14-15	-0.13278794315290554
1106	16-17	-0.13728614536708506
1106	18-19	-0.0988125058518392
1106	20-21	-0.0816041122289306
1106	22-23	-0.09344689331476985
1106	24-25	-0.10178511480400232
1106	26-27	-0.12114161305153459
1106	28-29	-0.19114422564734213
1106	30-31	-0.20349594118790293
1106	32-33	-0.17902463982419192
1106	34-35	-0.11601826898421308
1106	36-37	-0.17053297287794322
1106	38-39	-0.11982693938819011
1106	40-41	-0.10926453791936552
1106	42-43	-0.06683758827291086
1106	44-45	-0.042990898902267816
1106	46-47	-0.07062878595533206
1106	48-49	-0.06808473070891097
1106	50-51	0.03841076974682878
1106	52-53	0.024543445047797263
1106	54-55	-0.0232782933489446
1106	56-57	0.04875071255134955
1106	58-59	0.06300460442927758
1106	60-61	0.08309151198531595
1106	62-63	-0.02110518442066578
1106	64-65	0.05272077567185107
1106	66-67	0.07034643777675953
1106	68-69	0.11771983769085281
1106	70-71	0.1685903084299305
1106	72-73	0.13303624831968008
1106	74-75	0.2021675292395244
1106	76-77	0.24335608914588747
1106	78-79	0.24669441239241507
1106	80-81	0.27453046232815126
1106	82-83	0.34495019510782754
1106	84-85	0.335262769495678
1106	86-87	0.3310296179261094
1106	88-89	0.357417718498386
1106	90-91	0.3610765355565526
1106	92-93	0.2777650120609998
1106	94-95	0.30976178741005356
1106	96-97	0.18925395088466956
1106	98-99	0.19425929664451402
1106	100	0.19905923971891148
1107	1	-0.23150685923515013
1107	2	-0.22054462792034002
1107	3	-0.2160940912395617
1107	4	-0.15348855434584152
1107	5	-0.19023981865885986
1107	6	-0.28458386438754246
1107	7	-0.21955648675808703
1107	8	-0.14953522426648647
1107	9	-0.19152079303758285
1107	10-11	-0.16870950778384497
1107	12-13	-0.11999298736431285
1107	14-15	-0.1690432055255613
1107	16-17	-0.16574805065882003
1107	18-19	-0.11311496650107244
1107	20-21	-0.1165555880729201
1107	22-23	-0.12473672069505426
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2208	2	-0.6379749146215161
2208	3	-0.6119710010444166
2208	4	-0.5072131725160318
2208	5	-0.7075839021911889
2208	6	-0.6272590640404232
2208	7	-0.6090413987992775
2208	8	-0.5394569302170282
2208	9	-0.6934063384436797
2208	10-11	-0.6900229093618293
2208	12-13	-0.6851576943953859
2208	14-15	-0.7370348696708717
2208	16-17	-0.7118665277624459
2208	18-19	-0.7195224936687907
2208	20-21	-0.7432760639552214
2208	22-23	-0.6923018718540419
2208	24-25	-0.8150069840153549
2208	26-27	-0.8581094231672495
2208	28-29	-0.9726888755946774
2208	30-31	-1.0736074285207238
2208	32-33	-1.0378732132221913
2208	34-35	-0.93797227994515
2208	36-37	-0.9738201123890065
2208	38-39	-0.9140785359243893
2208	40-41	-0.9027501446493673
2208	42-43	-0.966530674703499
2208	44-45	-0.957845769433824
2208	46-47	-0.8813285326551323
2208	48-49	-0.7478964312427259
2208	50-51	-0.7018067715374485
2208	52-53	-0.6270793860620572
2208	54-55	-0.546772436156111
2208	56-57	-0.5565889534963766
2208	58-59	-1.041753851606952
2208	60-61	-0.664250100431282
2208	62-63	-0.5745761709698023
2208	64-65	-0.5108140050594798
2208	66-67	-0.40973545611670303
2208	68-69	-0.3798561146406989
2208	70-71	-0.2344560708695944
2208	72-73	-0.13451803834400167
2208	74-75	-0.11155122436896292
2208	76-77	-0.05232632921576652
2208	78-79	0.006017324965895909
2208	80-81	0.07196256215079799
2208	82-83	0.1529524180405133
2208	84-85	0.15704078641474695
2208	86-87	0.2027395952663369
2208	88-89	0.2833572672577134
2208	90-91	-0.5324247111742935
2208	92-93	-0.1473644672575425
2208	94-95	0.1583092664203214
2208	96-97	0.5664818279316641
2208	98-99	0.688998028120615
2208	100	0.5958912290907925
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	505622.0
3	153637.0
4	135149.0
5	125383.0
6	118795.0
7	135630.0
8	159714.0
9	175860.0
10	189403.0
11	205302.0
12	234553.0
13	293634.0
14	398116.0
15	483020.0
16	544245.0
17	598601.0
18	805952.0
19	665118.0
20	485421.0
21	447435.0
22	458960.0
23	487715.0
24	525010.0
25	567921.0
26	613522.0
27	658867.0
28	706643.0
29	763560.0
30	833998.0
31	911309.0
32	1012223.0
33	1164377.0
34	1360002.0
35	1689557.0
36	2254077.0
37	3125251.0
38	3022009.0
39	586411.0
40	536.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.42225278221744	33.18656769418582	25.53330907421009	21.85787044938665
2	22.16602580563716	24.74316342694041	32.2579637039139	20.832847063508524
3	22.892487277395663	32.38741799379065	22.78159160039476	21.938503128418926
4	23.089898496206114	22.09522810804672	32.242953224507445	22.57192017123972
5	28.947766263532916	22.330608887542972	22.928414117404056	25.793210731520062
6	28.731693765972683	22.468679820100256	25.93168644501937	22.867939968907695
7	24.155709947763064	27.835780158517824	22.18695077629114	25.821559117427967
8	27.803814962338592	22.781052287037873	27.158145292056012	22.256987458567522
9	23.192232182109244	26.809206651688783	22.43945753428828	27.559103631913693
10-11	29.559493190118214	22.52727957354386	22.556553312997252	25.356673923340672
12-13	27.089960998831724	22.617109866372928	22.80709841411484	27.485830720680504
14-15	26.43734085988625	23.06504900959231	23.932681761375154	26.564928369146283
16-17	25.810726383152517	22.95939319550097	24.585174740228	26.64470568111852
18-19	25.076083861161457	22.884818286248397	26.0614214144622	25.977676438127943
20-21	22.298385453569754	24.654519853549107	29.973617726530744	23.07347696635039
22-23	22.14469077642672	24.70722077178293	30.544941525691172	22.60314692609918
24-25	19.528271522816297	22.219504284819255	33.71509482095397	24.537129371410476
26-27	19.55657371290294	22.236834881858137	38.752158546521606	19.454432858717322
28-29	19.509845703103696	22.295698775306366	38.78761791060222	19.40683761098771
30-31	19.492323021926047	22.252825014984786	38.77530018538687	19.4795517777023
32-33	19.53491484972662	22.309586140570648	38.66842041507803	19.487078594624705
34-35	19.595351185710705	22.37296681408674	38.623559927610714	19.40812207259184
36-37	19.65852797454869	22.399251473851546	38.42370876820665	19.518511783393116
38-39	19.748541500844052	22.514057741328863	38.11760071793	19.61980003989708
40-41	19.884492842400586	22.715894307347053	37.68366616269207	19.71594668756029
42-43	20.10287973604271	22.885385063890872	37.01835326683786	19.993381933228555
44-45	20.303528490846727	23.245204111770878	36.26806963120539	20.183197766177006
46-47	20.622371157892147	23.630005077368857	35.38410401399883	20.363519750740164
48-49	21.06464977054365	24.326460629166675	33.73439559305815	20.874494007231526
50-51	21.57013130217547	25.443208553556858	31.59529386372362	21.39136628054405
52-53	21.861593165851232	26.309072693714686	30.144852333757612	21.684481806676466
54-55	21.99823542403375	26.857108575236545	29.207321694722776	21.937334306006928
56-57	22.10251171795136	27.394121706089365	28.529202780694696	21.97416379526458
58-59	22.197174913345304	27.7949292688364	28.064071934487682	21.943823883330612
60-61	22.19728996451426	28.1087254087462	27.683519322536966	22.01046530420258
62-63	22.30883639164672	28.444073553143497	27.3307438574676	21.91634619774219
64-65	22.370690671820228	28.817441172375446	26.95825574363631	21.853612412168015
66-67	22.431333969119514	29.075234360105558	26.607320222109227	21.8861114486657
68-69	22.501260229142947	29.40363828266797	26.20776058390934	21.887340904279743
70-71	22.67631191925938	29.66737532009796	25.867636641712515	21.78867611893014
72-73	22.761273955336	29.875825605554283	25.42994011468331	21.9329603244264
74-75	22.9342878761296	30.135628010212574	25.11308010896679	21.817004004691043
76-77	22.84550574281953	30.393680152715476	24.759585659571833	22.00122844489316
78-79	22.960805636530786	30.44359224225855	24.5199731611027	22.07562896010796
80-81	23.10021687148168	30.491289723161408	24.255387682910015	22.153105722446895
82-83	23.230861016496167	30.49581627620818	23.961754235760022	22.311568471535626
84-85	23.37835010785934	30.348416634479563	23.769298652413667	22.50393460524743
86-87	23.534653366682942	30.268544680897325	23.51433740298824	22.682464549431494
88-89	23.738011840178874	30.10648413379177	23.26279138530477	22.892712640724586
90-91	23.847541888554307	29.91157109903792	22.99601700755802	23.24487000484975
92-93	24.046342709626824	29.778463512414245	22.88621219596383	23.2889815819951
94-95	24.1833081778629	29.653999037796908	22.633402653263058	23.529290131077136
96-97	24.246803806080134	29.566773108259493	22.4052754128623	23.781147672798074
98-99	24.67106540386235	29.29659503909076	22.24156721091124	23.790772346135647
100	25.768997728798432	28.8193377419288	21.27507570551039	24.136588823762388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2973.0
1	2188.5
2	1438.0
3	1496.0
4	1481.5
5	1367.0
6	1389.0
7	1503.0
8	1722.0
9	2062.5
10	2663.0
11	3841.0
12	5077.5
13	7695.0
14	14499.0
15	25183.0
16	96921.0
17	149589.5
18	143064.0
19	152978.0
20	152112.0
21	147132.5
22	149006.0
23	154415.0
24	161241.5
25	172545.5
26	189074.5
27	209498.5
28	233352.0
29	260189.0
30	286527.5
31	316340.0
32	354372.0
33	394831.5
34	434308.5
35	483392.0
36	546730.0
37	623653.5
38	700681.0
39	760886.0
40	814875.0
41	878151.5
42	965357.5
43	1047967.0
44	1109078.0
45	1144699.0
46	1141410.5
47	1114533.0
48	1078785.5
49	1061008.0
50	1031679.0
51	972042.0
52	897614.5
53	812280.0
54	739570.0
55	675212.0
56	605811.0
57	541022.0
58	486154.5
59	435694.5
60	388686.0
61	342504.0
62	299840.5
63	261555.5
64	227692.0
65	198031.5
66	170057.0
67	145106.5
68	123424.5
69	103530.0
70	85458.0
71	70380.0
72	57931.5
73	47507.5
74	38673.0
75	30994.0
76	24885.5
77	19883.0
78	15729.0
79	12407.5
80	9640.5
81	7434.0
82	5641.0
83	4159.0
84	3050.5
85	2193.0
86	1538.5
87	1099.5
88	771.5
89	547.0
90	394.5
91	289.5
92	209.0
93	145.5
94	98.5
95	65.5
96	39.5
97	25.5
98	22.5
99	14.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17124874531465184
2	0.07287373356754367
3	0.06867484432047517
4	0.08824188558313008
5	0.09479200789434652
6	0.03805084880238187
7	0.03689515797424135
8	0.029859573058100674
9	0.038938448341235866
10-11	0.034591022028481584
12-13	0.028910384979815986
14-15	0.02950453324255907
16-17	0.029519024663601588
18-19	0.03131596087287336
20-21	0.03058233268259607
22-23	0.027684048474093215
24-25	0.029285350499291044
26-27	0.02116834328785273
28-29	0.02056694931458839
30-31	0.019503641295593906
32-33	0.019088824368251933
34-35	0.01642240289642931
36-37	0.01946378988772699
38-39	0.023668113417686445
40-41	0.02260480539869196
42-43	0.03034865851828553
44-45	0.031113080978278158
46-47	0.030872161103446358
48-49	0.027276477257272502
50-51	0.026716746119505388
52-53	0.027062728796895415
54-55	0.028281819592096927
56-57	0.02479482140374193
58-59	0.02046913222255142
60-61	0.010370423183549282
62-63	0.008542692704562167
64-65	0.006111756824680397
66-67	0.007477573257937368
68-69	0.012283290761161164
70-71	0.018574378921242675
72-73	0.016438705745102134
74-75	0.01595505456780822
76-77	0.021224497544392477
78-79	0.01912505292085822
80-81	0.01174891961021845
82-83	0.014413529654410765
84-85	0.017348042415519904
86-87	0.02178422868215959
88-89	0.025086461252222532
90-91	0.030198310024969442
92-93	0.08461721889487119
94-95	0.13271243390734577
96-97	0.08706808047868642
98-99	0.06943926678046779
100	0.0748554353951075
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.7602538E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.27111663859212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.93866846186329	32.945545452079834
2	11.45240092397714	9.682115504987156
3	3.923243095623769	4.975195994899907
4	1.9126531751120945	3.2339994181734757
5	1.1151159470917418	2.3568598132534566
6	0.7275404622113391	1.8452368642458437
7	0.5024255494560486	1.4866662302285751
8	0.3468157769390553	1.1728232127275802
9	0.26175653818613753	0.995826704092228
>10	1.5112013745283293	11.386173770180083
>50	0.13653980720848735	4.20298569135089
>100	0.1600583668534152	13.516668311805539
>500	0.00992123799142796	2.7285670177745978
>1k	0.0012150335700074379	0.8504356738931238
>5k	1.3162944826805974E-4	0.3629930014112936
>10k+	3.1261993958817835E-4	8.257907338896205
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	799331	2.895860518333495	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	663237	2.4028116544935108	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68408	0.2478322826690792	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA	48859	0.17700908517905128	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47040	0.1704191114599679	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	40767	0.14769294041004488	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	38939	0.1410703609936159	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	38788	0.14052330984926095	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG	36897	0.13367249055141234	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34474	0.1248943122549093	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA	31294	0.11337363252611048	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31272	0.11329392971037663	No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29342	0.1063018190573635	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC	28466	0.10312819784905286	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.086856578188571E-5	3.6228552606285696E-6	0.0	0.0	0.0
2	1.086856578188571E-5	3.6228552606285696E-6	0.0	7.245710521257139E-6	0.0
3	1.086856578188571E-5	2.173713156377142E-5	0.0	7.245710521257139E-6	0.0
4	1.086856578188571E-5	2.535998682439999E-5	0.0	7.245710521257139E-6	0.0
5	1.086856578188571E-5	2.535998682439999E-5	0.0	7.245710521257139E-6	0.0
6	1.086856578188571E-5	3.985140786691427E-5	0.0	7.245710521257139E-6	0.0
7	1.086856578188571E-5	5.071997364879998E-5	0.0	1.086856578188571E-5	0.0
8	1.086856578188571E-5	5.4342828909428546E-5	0.0	1.086856578188571E-5	0.0
9	1.4491421042514278E-5	5.4342828909428546E-5	0.0	1.086856578188571E-5	0.0
10-11	1.4491421042514278E-5	6.521139469131426E-5	0.0	1.4491421042514278E-5	3.6228552606285696E-6
12-13	1.6302848672828564E-5	6.521139469131426E-5	0.0	1.4491421042514278E-5	7.245710521257139E-6
14-15	5.071997364879997E-5	7.06456775822571E-5	0.0	1.4491421042514278E-5	9.057138151571424E-6
16-17	8.875995388539997E-5	7.789138810351425E-5	0.0	1.6302848672828564E-5	1.4491421042514278E-5
18-19	1.6302848672828564E-4	7.970281573382854E-5	0.0	1.8114276303142848E-5	1.4491421042514278E-5
20-21	3.351141116081427E-4	8.33256709944571E-5	0.0	1.8114276303142848E-5	1.4491421042514278E-5
22-23	8.731081178114852E-4	8.513709862477139E-5	0.0	2.535998682439999E-5	1.4491421042514278E-5
24-25	0.0017117991106469993	9.057138151571424E-5	0.0	2.8982842085028557E-5	1.4491421042514278E-5
26-27	0.002822204248029656	9.238280914602852E-5	0.0	2.8982842085028557E-5	1.4491421042514278E-5
28-29	0.005729545594684083	9.781709203697139E-5	1.8114276303142848E-6	3.260569734565713E-5	1.4491421042514278E-5
30-31	0.008022812974661966	9.781709203697139E-5	3.6228552606285696E-6	3.260569734565713E-5	1.6302848672828564E-5
32-33	0.008566241263756252	1.0868565781885709E-4	7.245710521257139E-6	3.6228552606285695E-5	1.8114276303142848E-5
34-35	0.009404932256591766	1.1774279597042851E-4	7.245710521257139E-6	3.985140786691427E-5	1.8114276303142848E-5
36-37	0.010600474492599194	1.2861136175231424E-4	7.245710521257139E-6	3.985140786691427E-5	1.9925703933457135E-5
38-39	0.012252496491445823	1.3223421701294278E-4	9.057138151571424E-6	3.985140786691427E-5	2.3548559194085702E-5
40-41	0.014360998253131651	1.3404564464325708E-4	1.086856578188571E-5	4.347426312754284E-5	2.535998682439999E-5
42-43	0.01741325381021122	1.3947992753419992E-4	1.2679993412199993E-5	4.347426312754284E-5	2.535998682439999E-5
44-45	0.021604897346758474	1.412913551645142E-4	1.4491421042514278E-5	4.347426312754284E-5	2.535998682439999E-5
46-47	0.0274666771584555	1.4853706568577135E-4	1.6302848672828564E-5	4.347426312754284E-5	2.535998682439999E-5
48-49	0.03539348446871081	1.5397134857671424E-4	1.8114276303142848E-5	4.528569075785712E-5	2.8982842085028557E-5
50-51	0.04624574740192369	1.6121705909797137E-4	1.8114276303142848E-5	4.7097118388171404E-5	2.8982842085028557E-5
52-53	0.06010135734619766	1.684627696192285E-4	1.8114276303142848E-5	5.071997364879998E-5	3.0794269715342844E-5
54-55	0.07942747873402076	1.7933133540111418E-4	1.8114276303142848E-5	5.4342828909428546E-5	3.260569734565713E-5
56-57	0.10434728864425438	1.9382275644362848E-4	1.9925703933457135E-5	5.796568417005711E-5	3.8039980236599986E-5
58-59	0.13561615239873956	1.9925703933457135E-4	2.173713156377142E-5	6.158853943068569E-5	3.985140786691427E-5
60-61	0.17552733737745418	2.0469132222551418E-4	2.173713156377142E-5	7.06456775822571E-5	3.985140786691427E-5
62-63	0.22417322638954434	2.1374846037708562E-4	2.7171414454714273E-5	7.245710521257139E-5	4.347426312754284E-5
64-65	0.2855226573730285	2.2099417089834275E-4	2.8982842085028557E-5	7.245710521257139E-5	4.347426312754284E-5
66-67	0.3607892868402174	2.2823988141959989E-4	2.8982842085028557E-5	7.607996047319997E-5	4.347426312754284E-5
68-69	0.4526504048287154	2.3005130904991418E-4	3.260569734565713E-5	8.151424336414282E-5	4.7097118388171404E-5
70-71	0.5620081022984191	2.3729701957117132E-4	3.260569734565713E-5	8.33256709944571E-5	5.071997364879998E-5
72-73	0.6911665151950883	2.4454273009242845E-4	3.260569734565713E-5	8.513709862477139E-5	5.071997364879998E-5
74-75	0.8400948492490075	2.535998682439999E-4	3.260569734565713E-5	9.60056644066571E-5	5.071997364879998E-5
76-77	1.008956132946905	2.590341511349427E-4	3.260569734565713E-5	9.962851966728567E-5	5.071997364879998E-5
78-79	1.2032317462981121	2.6627986165619986E-4	3.260569734565713E-5	1.0143994729759996E-4	5.071997364879998E-5
80-81	1.4193133254630426	2.771484274380856E-4	3.8039980236599986E-5	1.0143994729759996E-4	5.253140127911426E-5
82-83	1.6578493615333487	2.843941379593427E-4	3.985140786691427E-5	1.0325137492791424E-4	5.4342828909428546E-5
84-85	1.9206675849880182	2.9163984848059986E-4	3.985140786691427E-5	1.0868565781885709E-4	5.4342828909428546E-5
86-87	2.2074165788667695	2.952627037412284E-4	4.347426312754284E-5	1.1411994070979994E-4	5.4342828909428546E-5
88	2.437638162113933	2.970741313715427E-4	5.071997364879998E-5	1.1955422360074281E-4	5.4342828909428546E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	130540	0.0	85.52878	1
TATCTCG	130100	0.0	84.9739	2
TATGGAT	154565	0.0	83.23825	2
ATGGATC	158205	0.0	81.18829	3
ATATGGA	159800	0.0	80.85949	1
GGATCCG	161565	0.0	78.98636	5
TGGATCC	164530	0.0	77.852806	4
ATCTCGA	144805	0.0	75.7657	3
TCTCGAG	151180	0.0	72.35927	4
GATCCGG	177960	0.0	71.635445	6
CTCGAGG	160890	0.0	67.91607	5
ATCCGGC	186375	0.0	67.496025	7
TCGAGGG	170360	0.0	63.9893	6
TCCGGCG	199340	0.0	63.0536	8
CCGGCGC	213725	0.0	58.761333	9
CGAGGGC	187625	0.0	57.836494	7
AGGGCGC	200950	0.0	53.801693	9
GAGGGCG	203405	0.0	53.293274	8
CCGTCGA	191840	0.0	30.459623	16-17
GTCGACT	191970	0.0	30.289644	18-19
>>END_MODULE