FastQCFastQC Report
Wed 25 May 2016
SRR490976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33261944
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT8976482.698723802793968No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT5728011.7220911682131388No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA686300.20633189689694625No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA614390.1847125952710401No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG610010.1833957750635381No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC503990.15152151058879781No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA474730.14272467057247165No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG404320.12155633477105246No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA382970.1151375878691877No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG358280.10771469039813188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG1385150.086.5325242
ATATCTC1419500.084.9826351
TATGGAT1618800.082.93252
GGATCCG1625650.081.841655
ATGGATC1636000.081.7695163
ATCTCGA1473050.080.707353
TGGATCC1676000.079.497264
ATATGGA1714450.078.913751
TCTCGAG1516400.078.259764
GATCCGG1719900.077.429646
ATCCGGC1790700.074.308347
CTCGAGG1600650.074.180435
TCCGGCG1859950.071.795218
TCGAGGG1675650.070.8218466
CCGGCGC1979450.067.961859
CGAGGGC1828950.065.004797
AGGGCGC1872150.063.7867139
GAGGGCG1941500.061.467118
CCGTCGA1841600.036.0706416-17
GTCGACT1850550.035.84412818-19
Produced by FastQC (version 0.11.5)