##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490976_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33261944
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15557740702107	33.0	31.0	34.0	28.0	34.0
2	31.416793347977496	34.0	31.0	34.0	28.0	34.0
3	31.528307004545496	34.0	31.0	34.0	28.0	34.0
4	34.89583368909526	37.0	35.0	37.0	32.0	37.0
5	34.71548920892898	37.0	35.0	37.0	32.0	37.0
6	34.625945975977835	37.0	35.0	37.0	31.0	37.0
7	34.623501530758396	37.0	35.0	37.0	32.0	37.0
8	34.54626957462258	37.0	35.0	37.0	31.0	37.0
9	36.04712986709376	39.0	37.0	39.0	31.0	39.0
10-11	35.97112724379549	39.0	37.0	39.0	30.5	39.0
12-13	35.83984586108377	39.0	37.0	39.0	30.0	39.0
14-15	36.946310564409586	40.0	37.0	41.0	30.5	41.0
16-17	36.816562465501114	40.0	37.0	41.0	30.0	41.0
18-19	36.72965866035972	40.0	37.0	41.0	30.0	41.0
20-21	36.4396446732037	40.0	36.5	41.0	29.0	41.0
22-23	36.25588734380648	40.0	36.0	41.0	28.0	41.0
24-25	36.19595971299813	40.0	36.0	41.0	27.5	41.0
26-27	36.141389661410045	40.0	36.0	41.0	27.5	41.0
28-29	36.18117842120112	40.0	36.0	41.0	28.0	41.0
30-31	35.99035411159372	39.5	36.0	41.0	27.5	41.0
32-33	35.41550137598692	39.0	35.0	41.0	26.5	41.0
34-35	35.151775930474784	39.0	35.0	41.0	25.0	41.0
36-37	34.942801178427814	38.5	35.0	41.0	24.5	41.0
38-39	34.72991142670435	38.0	35.0	41.0	23.5	41.0
40-41	34.437262701783155	38.0	35.0	41.0	21.5	41.0
42-43	34.0827770619781	38.0	34.0	41.0	18.5	41.0
44-45	33.649944362843016	38.0	33.5	40.5	12.0	41.0
46-47	32.94315021094377	38.0	33.0	40.0	4.5	41.0
48-49	31.88722079803874	38.0	31.0	40.0	2.0	41.0
50-51	31.01598962465934	37.5	29.5	40.0	2.0	41.0
52-53	30.700602135581732	38.0	29.0	40.0	2.0	41.0
54-55	30.400187012520977	37.0	28.0	40.0	2.0	41.0
56-57	29.955592884168166	37.0	27.5	40.0	2.0	41.0
58-59	29.533578344067923	36.0	26.0	40.0	2.0	41.0
60-61	29.07461985685503	36.0	25.5	40.0	2.0	41.0
62-63	28.590534771509446	35.0	23.5	39.5	2.0	41.0
64-65	28.04104881843346	35.0	21.5	39.0	2.0	41.0
66-67	27.483661733661748	34.5	19.0	39.0	2.0	40.5
68-69	26.868672032518603	34.0	14.0	38.0	2.0	40.0
70-71	26.2642535715892	34.0	7.5	37.5	2.0	40.0
72-73	25.65282654555609	33.5	2.0	37.0	2.0	39.5
74-75	25.022947681590708	33.0	2.0	36.0	2.0	39.0
76-77	24.387319153685063	32.0	2.0	35.5	2.0	39.0
78-79	24.0511711822977	32.0	2.0	35.0	2.0	37.5
80-81	23.49944946092147	32.0	2.0	35.0	2.0	37.0
82-83	22.880448749477782	31.0	2.0	35.0	2.0	36.5
84-85	22.363673136482944	31.0	2.0	35.0	2.0	36.0
86-87	21.775718295358807	30.5	2.0	35.0	2.0	36.0
88-89	21.128774403564627	29.5	2.0	34.5	2.0	35.0
90-91	20.456277961384338	29.0	2.0	34.0	2.0	35.0
92-93	19.83339754585601	28.0	2.0	34.0	2.0	35.0
94-95	19.08514439204155	26.5	2.0	34.0	2.0	35.0
96-97	18.35156619228269	25.0	2.0	34.0	2.0	35.0
98-99	17.628710095838052	22.0	2.0	34.0	2.0	35.0
100	16.654626350161614	17.0	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15213418824057712
1101	2	0.1714546269876145
1101	3	0.2276558058611471
1101	4	0.2448598043139114
1101	5	0.24166804447067136
1101	6	0.26039568228980414
1101	7	0.2910805292487524
1101	8	0.2882465770398497
1101	9	0.3435184785384138
1101	10-11	0.3434795540053699
1101	12-13	0.31196022993388084
1101	14-15	0.3614474967889336
1101	16-17	0.30657279987179464
1101	18-19	0.3080473125132386
1101	20-21	0.2428724939219009
1101	22-23	0.256163390790789
1101	24-25	0.28475379903603226
1101	26-27	0.30957769994247286
1101	28-29	0.37138807722530487
1101	30-31	0.36635039974314765
1101	32-33	0.37059625419428954
1101	34-35	0.32324845546296643
1101	36-37	0.29195092542612855
1101	38-39	0.17861953268999287
1101	40-41	0.1960042068618577
1101	42-43	0.1916582437958283
1101	44-45	0.1350015283450503
1101	46-47	0.15328571145550995
1101	48-49	0.1678823615882159
1101	50-51	0.11162893933932949
1101	52-53	0.03676236313763681
1101	54-55	-0.007686529478927895
1101	56-57	-0.05490689127790205
1101	58-59	-0.12363890793577426
1101	60-61	-0.13552971392628166
1101	62-63	-0.2346712800497599
1101	64-65	-0.21958217465575203
1101	66-67	-0.3256571198481666
1101	68-69	-0.36933396759844683
1101	70-71	-0.44958425674346003
1101	72-73	-0.5993352639871112
1101	74-75	-0.5917268307844026
1101	76-77	-0.6845431503322033
1101	78-79	-0.7931652408164318
1101	80-81	-0.7690960459183458
1101	82-83	-0.769071138224561
1101	84-85	-0.8851174964883839
1101	86-87	-1.0040308634625674
1101	88-89	-1.1269804894254278
1101	90-91	-1.2360682542395018
1101	92-93	-1.3020162948795289
1101	94-95	-1.3901821401012953
1101	96-97	-1.60631132391714
1101	98-99	-1.6300595798330342
1101	100	-1.5258022381500211
1102	1	0.16500067401199914
1102	2	0.2057629488022421
1102	3	0.22780907608508016
1102	4	0.2526942552757845
1102	5	0.2593587766895382
1102	6	0.31370748138909477
1102	7	0.289816617559552
1102	8	0.3194159191882875
1102	9	0.3404838364333216
1102	10-11	0.3317459598119825
1102	12-13	0.3338867397844254
1102	14-15	0.41504239138055965
1102	16-17	0.37659273329368403
1102	18-19	0.40719599462178024
1102	20-21	0.3677521475350787
1102	22-23	0.34949105959238835
1102	24-25	0.3499705429844653
1102	26-27	0.3999666405471487
1102	28-29	0.3991547295680391
1102	30-31	0.37320769321714664
1102	32-33	0.38545492093820144
1102	34-35	0.40534111372059556
1102	36-37	0.3694875654673737
1102	38-39	0.3686118018277469
1102	40-41	0.36979919626405433
1102	42-43	0.3592768811689666
1102	44-45	0.29744154307979187
1102	46-47	0.28091249696571197
1102	48-49	0.2946547599900775
1102	50-51	0.3247510568952947
1102	52-53	0.2366263971861926
1102	54-55	0.18400731698688588
1102	56-57	0.17442414488192526
1102	58-59	0.113670470496114
1102	60-61	0.05775154751350087
1102	62-63	-0.01403193717771245
1102	64-65	-0.026482599182234168
1102	66-67	-0.04423876249055425
1102	68-69	-0.07221983082976635
1102	70-71	-0.13108943311773302
1102	72-73	-0.18859771845251316
1102	74-75	-0.21404658974861945
1102	76-77	-0.30250624647403157
1102	78-79	-0.37071231091044154
1102	80-81	-0.43862799475738257
1102	82-83	-0.4634309615662815
1102	84-85	-0.5510873264841969
1102	86-87	-0.6426440918908227
1102	88-89	-0.7055505366945987
1102	90-91	-0.7574435626000451
1102	92-93	-0.845382935526569
1102	94-95	-0.9205746521157963
1102	96-97	-1.0707806597637877
1102	98-99	-1.0929190168045402
1102	100	-1.0201733635820638
1103	1	0.03560011302256427
1103	2	0.026721035725014275
1103	3	0.0069564972635696165
1103	4	0.011876886383575425
1103	5	0.06748144811014356
1103	6	0.08549115432329302
1103	7	0.06598495407700966
1103	8	0.10720519897007819
1103	9	0.050002542936447014
1103	10-11	0.09995530922908813
1103	12-13	0.07881238469642682
1103	14-15	0.1437479764331826
1103	16-17	0.11719434346321833
1103	18-19	0.11460265179510998
1103	20-21	0.1367155869115635
1103	22-23	0.1544664425538116
1103	24-25	0.1318307378123862
1103	26-27	0.06362868519329368
1103	28-29	0.12799225407164272
1103	30-31	0.0785638347878006
1103	32-33	0.04222169879339788
1103	34-35	0.07898523656177758
1103	36-37	0.05972116762008994
1103	38-39	0.023129829815978553
1103	40-41	0.051166607149767174
1103	42-43	0.09333548399352054
1103	44-45	0.02208207810050311
1103	46-47	-0.03733878168877425
1103	48-49	-0.10949802116990881
1103	50-51	-0.06957852896626449
1103	52-53	-0.010004925823643873
1103	54-55	-0.06960288233305079
1103	56-57	-0.04692166562101008
1103	58-59	-0.1344817993430496
1103	60-61	-0.16772503425432816
1103	62-63	-0.24747685332911473
1103	64-65	-0.2968601076458448
1103	66-67	-0.2910513098790837
1103	68-69	-0.2810816424677789
1103	70-71	-0.28758609590039796
1103	72-73	-0.38035751416216357
1103	74-75	-0.36132386982181686
1103	76-77	-0.320002357048331
1103	78-79	-0.3794155201821994
1103	80-81	-0.359203287779156
1103	82-83	-0.3921166065355095
1103	84-85	-0.45214423592915054
1103	86-87	-0.5268755139333301
1103	88-89	-0.4882948092091084
1103	90-91	-0.49563913222085887
1103	92-93	-0.5302483105842413
1103	94-95	-0.6473428210128418
1103	96-97	-0.7693984866343762
1103	98-99	-0.7647478936551444
1103	100	-0.7318098064095899
1104	1	-0.0395374076189654
1104	2	-0.008082672198423069
1104	3	-0.03241859990459872
1104	4	0.04289728834488926
1104	5	-0.05774890967597912
1104	6	-0.012294835151728023
1104	7	-0.02609806599363651
1104	8	-0.03196293967355501
1104	9	-0.05506382931245213
1104	10-11	-0.04179516201244837
1104	12-13	8.566123141449111E-4
1104	14-15	0.08582282277726705
1104	16-17	0.06859074934116194
1104	18-19	0.11252711681080996
1104	20-21	0.11203448596605625
1104	22-23	0.07276862732540934
1104	24-25	-0.06311497526010612
1104	26-27	0.02369509880358578
1104	28-29	0.07530222254266761
1104	30-31	0.03013089939463498
1104	32-33	0.08735156267661637
1104	34-35	0.12833645244950276
1104	36-37	0.047039197408153655
1104	38-39	0.10499063704664024
1104	40-41	0.07988521283633787
1104	42-43	0.03919806303871809
1104	44-45	0.06081540903375071
1104	46-47	-0.03361743863612077
1104	48-49	0.092874106771756
1104	50-51	-0.019161705019993036
1104	52-53	0.0019330284786249763
1104	54-55	0.04900019920582821
1104	56-57	0.030592658564380315
1104	58-59	0.007989234862602501
1104	60-61	0.024256314688226865
1104	62-63	-0.0185786026875725
1104	64-65	0.04314220714564598
1104	66-67	-0.007870495927960519
1104	68-69	0.019163293506615275
1104	70-71	0.10449717556955562
1104	72-73	0.02632846569426306
1104	74-75	0.1117070854243778
1104	76-77	0.09331174328960046
1104	78-79	0.02340156028220619
1104	80-81	0.06968140224843822
1104	82-83	0.011777767571590658
1104	84-85	0.050695364272502985
1104	86-87	0.008738551410189643
1104	88-89	0.03827483923883079
1104	90-91	-0.02951099419327008
1104	92-93	-0.03068071514349313
1104	94-95	0.023126101069603777
1104	96-97	-0.09223229179537284
1104	98-99	-0.15752778031700032
1104	100	-0.09101391089327393
1105	1	-0.04247497949412349
1105	2	-0.02987125585104522
1105	3	-0.03159355878377568
1105	4	-0.026075538456360903
1105	5	-0.057647260778153964
1105	6	0.001878063707806632
1105	7	-0.034407784893318194
1105	8	-0.02694478135990863
1105	9	0.0042861988818287955
1105	10-11	0.001984074880894582
1105	12-13	0.04647008068186409
1105	14-15	0.020981104441155196
1105	16-17	-0.009949148812715691
1105	18-19	-0.01894483331949459
1105	20-21	-0.005368727722732558
1105	22-23	-0.0167717351191925
1105	24-25	-0.018288363128476703
1105	26-27	-0.01845829124173548
1105	28-29	-0.0907558451793875
1105	30-31	-0.027579959632220152
1105	32-33	0.0037408514427355044
1105	34-35	-0.08940099884772224
1105	36-37	-0.0839027950417588
1105	38-39	-0.045120568079191514
1105	40-41	0.04283052285937572
1105	42-43	0.023272734173339416
1105	44-45	0.07649796686288113
1105	46-47	0.07098353745995212
1105	48-49	0.1437596253975677
1105	50-51	0.2692055379187863
1105	52-53	0.20406914144428612
1105	54-55	0.17646790427351533
1105	56-57	0.10580993945185924
1105	58-59	0.24663788737686687
1105	60-61	0.21867797956150525
1105	62-63	0.23482741839186616
1105	64-65	0.2271246820156847
1105	66-67	0.2620909753250018
1105	68-69	0.29752798299457694
1105	70-71	0.32116963720617875
1105	72-73	0.22670780947077773
1105	74-75	0.24553968947309812
1105	76-77	0.33900347148083654
1105	78-79	0.3445741270176619
1105	80-81	0.327932465690175
1105	82-83	0.36118194067861253
1105	84-85	0.41950118640816925
1105	86-87	0.4072942726794224
1105	88-89	0.46969699635678097
1105	90-91	0.449155492739429
1105	92-93	0.4333146383051769
1105	94-95	0.43862591445186894
1105	96-97	0.3818627004259447
1105	98-99	0.4289414029862648
1105	100	0.4485849466495182
1106	1	-0.2212698337968142
1106	2	-0.191756559768141
1106	3	-0.17357962979689745
1106	4	-0.10575442884500319
1106	5	-0.1448739426439687
1106	6	-0.08345719448755062
1106	7	-0.16230614360419082
1106	8	-0.13127881993620605
1106	9	-0.22265535887569143
1106	10-11	-0.1577647729027163
1106	12-13	-0.15260908188857059
1106	14-15	-0.1619843103851153
1106	16-17	-0.1717551857901256
1106	18-19	-0.14745750434637728
1106	20-21	-0.14902327709084773
1106	22-23	-0.14928341182445592
1106	24-25	-0.1600022037674762
1106	26-27	-0.15261381169727173
1106	28-29	-0.22759583895685864
1106	30-31	-0.2449088636209069
1106	32-33	-0.2207685999719189
1106	34-35	-0.1435664555898768
1106	36-37	-0.20735971481530413
1106	38-39	-0.16256650808397666
1106	40-41	-0.13007536195641478
1106	42-43	-0.14475587007713386
1106	44-45	-0.12863366174216395
1106	46-47	-0.1487858482382336
1106	48-49	-0.17401465914737102
1106	50-51	-0.05207701317341318
1106	52-53	-0.06580008362819001
1106	54-55	-0.08110298781432235
1106	56-57	-0.0055498753625649044
1106	58-59	0.014261907696834442
1106	60-61	0.027777615685955936
1106	62-63	-0.06441948022177257
1106	64-65	0.018601246280304906
1106	66-67	-0.033045330204114265
1106	68-69	0.004544810453648296
1106	70-71	0.08098471754036396
1106	72-73	0.054698308532984186
1106	74-75	0.159801919352887
1106	76-77	0.17834449870665736
1106	78-79	0.189498338044654
1106	80-81	0.21387622491052483
1106	82-83	0.25469521876732415
1106	84-85	0.23558889856874643
1106	86-87	0.22956955764917808
1106	88-89	0.28955085926599367
1106	90-91	0.3298096482473518
1106	92-93	0.23908845169742676
1106	94-95	0.31678224497866836
1106	96-97	0.17755281073254636
1106	98-99	0.2043374614685085
1106	100	0.20526883595466572
1107	1	-0.273264627253738
1107	2	-0.26489804388226545
1107	3	-0.2831369157787478
1107	4	-0.19760409753393304
1107	5	-0.24350014009350218
1107	6	-0.3505725529042394
1107	7	-0.29062755106445337
1107	8	-0.2240183516805203
1107	9	-0.29941222009595947
1107	10-11	-0.253326367089862
1107	12-13	-0.257346674670913
1107	14-15	-0.29744889845022016
1107	16-17	-0.29814516247977707
1107	18-19	-0.23168225441193613
1107	20-21	-0.2611161925870178
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2208	1	-0.9078006229854658
2208	2	-0.6647922602767693
2208	3	-0.6541878185684453
2208	4	-0.5486980290561405
2208	5	-0.7799695962049995
2208	6	-0.6944536065981097
2208	7	-0.6620591863312839
2208	8	-0.6406438985716676
2208	9	-0.7521073913552812
2208	10-11	-0.7538944335881865
2208	12-13	-0.7871227446172
2208	14-15	-0.9040823062327661
2208	16-17	-0.8549737968962674
2208	18-19	-0.8881431252626868
2208	20-21	-0.8822996198961093
2208	22-23	-0.8451631093922174
2208	24-25	-0.9585287806744205
2208	26-27	-0.9851374524327525
2208	28-29	-1.0654091515218198
2208	30-31	-1.13892598237436
2208	32-33	-1.1309184886614077
2208	34-35	-1.0514095479196754
2208	36-37	-1.071489608229932
2208	38-39	-1.0474558081687917
2208	40-41	-0.9785047024326659
2208	42-43	-1.0482142210932466
2208	44-45	-1.0569116072378648
2208	46-47	-0.993358036666173
2208	48-49	-0.8844435621117093
2208	50-51	-0.8596051853877178
2208	52-53	-0.7656959054637014
2208	54-55	-0.6713920452986173
2208	56-57	-0.6701495720056201
2208	58-59	-1.158922235447264
2208	60-61	-0.7856665121290263
2208	62-63	-0.702952133136101
2208	64-65	-0.6104430701771584
2208	66-67	-0.5148903722339568
2208	68-69	-0.49191925801724423
2208	70-71	-0.33158762943957854
2208	72-73	-0.2181244689116646
2208	74-75	-0.2179931657945282
2208	76-77	-0.15929927359783136
2208	78-79	-0.1212883386944057
2208	80-81	-0.04904228554218193
2208	82-83	0.052482091987236856
2208	84-85	0.07717662287243243
2208	86-87	0.11371784202984259
2208	88-89	0.13889770898397202
2208	90-91	-0.6735313537754841
2208	92-93	-0.2755220078775906
2208	94-95	0.004734744410320957
2208	96-97	0.4488971103370609
2208	98-99	0.5577438594363535
2208	100	0.45878534291158246
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	519722.0
3	147639.0
4	120691.0
5	118564.0
6	124796.0
7	137933.0
8	148841.0
9	164023.0
10	186371.0
11	210421.0
12	237631.0
13	281691.0
14	353929.0
15	466451.0
16	579602.0
17	737089.0
18	1102279.0
19	813190.0
20	550434.0
21	490083.0
22	492067.0
23	512254.0
24	544083.0
25	581945.0
26	627816.0
27	683309.0
28	749502.0
29	828290.0
30	925243.0
31	1039125.0
32	1188245.0
33	1391121.0
34	1690337.0
35	2150486.0
36	2907972.0
37	4136773.0
38	4316110.0
39	1004446.0
40	1440.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.413626810457917	34.12619224818333	26.358422260935438	21.101758680423323
2	21.431211987963415	26.00886271190801	32.569652410017085	19.990272890111488
3	21.792441721196447	32.84345035235436	24.002255342267535	21.361852584181666
4	22.143544325181477	23.714016550142862	32.631998834616496	21.51044029005916
5	26.787105765168345	24.098353665008666	24.393309691549444	24.72123087827354
6	26.791728472774402	24.206571689455636	27.26738950099564	21.734310336774325
7	22.657062025994307	28.741369906438365	23.867328557179036	24.734239510388292
8	26.03469137288713	24.387869312234535	28.25655023570861	21.32088907916972
9	21.815076715789385	27.89791335254483	24.150791038669684	26.136218892996112
10-11	27.952418066812722	24.11341510123142	24.02233336406033	23.911833467895526
12-13	25.739773686502375	24.218743480127856	24.17043167393529	25.871051159434476
14-15	25.450004972696483	24.464726890998282	24.53834294836603	25.546925187939205
16-17	25.161196938658097	24.366694412731647	24.907711102413778	25.564397546196478
18-19	24.817763254963506	24.329388146117086	25.578515314647287	25.27433328427212
20-21	22.360840067690933	26.05815200866858	28.503393719251047	23.077614204389445
22-23	22.460459696165795	26.200314984470687	28.757513187394046	22.581712131969468
24-25	20.161922769811714	23.917064149617655	31.399292561897376	24.52172051867326
26-27	20.227661435461112	23.95787676754345	35.67438431156461	20.14007748543083
28-29	20.18084667649599	24.040880566265486	35.689245016291515	20.08902774094701
30-31	20.18990424874694	23.98714123012502	35.65535821646145	20.167596304666585
32-33	20.235232900767244	24.020313723454823	35.575260253681876	20.16919312209605
34-35	20.256186344283844	24.073282347167417	35.60968745772373	20.060843850825005
36-37	20.280993756492848	24.045863722744702	35.53489328344028	20.138249237322164
38-39	20.285502402224395	24.102240157002655	35.46871925366061	20.14353818711234
40-41	20.34616340084834	24.209473712414585	35.30433025115768	20.140032635579395
42-43	20.469337409610002	24.256109757286037	34.941995204723	20.332557628380965
44-45	20.579936446865798	24.47834279761395	34.52237611148476	20.41934464403549
46-47	20.88751640886502	24.749272061260204	33.793480278129906	20.56973125174487
48-49	21.336600873292056	25.348478535417136	32.26161862016607	21.053301971124736
50-51	21.796856668413152	26.188097526325688	30.47264702165246	21.5423987836087
52-53	22.090071365732868	26.56509137441283	29.48855623196533	21.856281027888976
54-55	22.208917069228328	26.629874934686153	28.970522942785408	22.190685053300115
56-57	22.32678789413393	26.75068551977296	28.660380877815655	22.26214570827746
58-59	22.440552597623267	26.832043844456155	28.4648892249622	22.26251433295838
60-61	22.41431418536696	26.81612267944392	28.370042357404667	22.399520777784453
62-63	22.497454768652034	26.845664997567788	28.331989000974467	22.324891232805715
64-65	22.567536302561912	26.91287099509752	28.228885373457164	22.290707328883403
66-67	22.6550382335192	26.89454759946333	28.114188788913445	22.33622537810403
68-69	22.743267979213503	26.98846960761835	27.88627884915245	22.3819835640157
70-71	22.913633058072318	27.128001002188405	27.684629338235702	22.273736601503572
72-73	23.01396786982945	27.240513264951595	27.353853709683047	22.39166515553591
74-75	23.147242300467617	27.429745836731577	27.152037745327235	22.270974117473575
76-77	23.03618559038991	27.67967116275062	26.8968330145107	22.387310232348767
78-79	23.075390756117738	27.759155230811388	26.737595844865037	22.427858168205837
80-81	23.146795757431146	27.911999511330233	26.514578976282245	22.42662575495638
82-83	23.16596629018828	28.08154487344549	26.270873709527265	22.481615126838964
84-85	23.206011162866503	28.11135021730799	26.106967221802996	22.57567139802251
86-87	23.28157087504012	28.203209385281852	25.91195852347552	22.603261216202508
88-89	23.40461970603019	28.21758847609641	25.69241768526986	22.685374132603542
90-91	23.443097843680963	28.207966596157075	25.44247209753989	22.906463462622074
92-93	23.576028587497667	28.23257922655758	25.344145350251278	22.847246835693475
94-95	23.660520537499952	28.283079189578704	25.098174435082676	22.958225837838672
96-97	23.676687483972664	28.360551242246775	24.83616083467746	23.126600439103107
98-99	24.010934916924363	28.23757313307477	24.654764562159933	23.096727387840932
100	24.91864658608716	27.940903159116555	23.798736791456555	23.34171346333973
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2403.0
1	2103.5
2	1896.0
3	1910.5
4	1724.5
5	1579.0
6	1624.0
7	1794.0
8	2067.5
9	2464.0
10	2967.5
11	3654.0
12	4569.5
13	6311.0
14	10317.0
15	15089.0
16	59990.5
17	86172.0
18	72007.0
19	79515.5
20	93401.0
21	106510.0
22	118802.5
23	136584.5
24	157699.0
25	182817.5
26	212609.0
27	246912.0
28	285305.5
29	327553.0
30	372562.5
31	425532.0
32	490215.0
33	558810.5
34	622781.5
35	697870.0
36	788943.0
37	894002.5
38	997246.0
39	1068412.0
40	1123885.5
41	1188353.5
42	1276940.0
43	1356475.0
44	1404523.0
45	1422453.0
46	1401209.5
47	1351730.0
48	1295328.5
49	1262524.0
50	1207340.5
51	1115192.5
52	1014244.0
53	908411.0
54	818269.5
55	740190.5
56	662144.5
57	590728.0
58	531285.5
59	477515.0
60	425495.5
61	373704.5
62	326581.5
63	285629.5
64	250177.0
65	217350.5
66	186697.0
67	159576.0
68	135723.5
69	114351.5
70	94881.5
71	78203.0
72	64278.5
73	52945.0
74	43511.0
75	35190.5
76	28136.5
77	22567.0
78	18002.0
79	14224.0
80	11254.0
81	8704.5
82	6598.5
83	4835.0
84	3425.5
85	2379.5
86	1667.5
87	1163.5
88	772.5
89	537.0
90	376.5
91	250.5
92	176.0
93	116.5
94	72.5
95	52.0
96	32.0
97	17.5
98	10.0
99	4.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17681167402602807
2	0.07497757797920651
3	0.07074150566785874
4	0.0900879395383505
5	0.0989809855972339
6	0.04051777611074085
7	0.038299024254264874
8	0.0315886527858985
9	0.0404155571905238
10-11	0.03649516095631692
12-13	0.030778417521236882
14-15	0.03116775134971065
16-17	0.031212847932159348
18-19	0.03296860820882869
20-21	0.03251162950668187
22-23	0.02954878403980236
24-25	0.03130604753588666
26-27	0.02273920009004886
28-29	0.022143925201726034
30-31	0.020968407619229953
32-33	0.021005988104603867
34-35	0.018023600785329923
36-37	0.02127656759929606
38-39	0.02553518820186818
40-41	0.02421085189729139
42-43	0.0321222956782081
44-45	0.03267848686174205
46-47	0.032658945009347615
48-49	0.028888870716636404
50-51	0.02852058195997203
52-53	0.02856267210359082
54-55	0.03005987864088762
56-57	0.026731750856173648
58-59	0.021931971264217146
60-61	0.01168452451245784
62-63	0.009558972259709173
64-65	0.006731416540175764
66-67	0.008348880630669091
68-69	0.013497407126895532
70-71	0.02020326893701703
72-73	0.017865762746759477
74-75	0.017440351652326755
76-77	0.022730180773559115
78-79	0.020162682012813203
80-81	0.012959254576341058
82-83	0.015595901430174978
84-85	0.018486592365136566
86-87	0.023038340753625226
88-89	0.026358952441264405
90-91	0.03138872460370927
92-93	0.0862096334477624
94-95	0.13543405640993203
96-97	0.08916496281756713
98-99	0.07109776866920346
100	0.07752102522931312
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.3261944E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.939970595929815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.44038362145714	34.027281792643514
2	11.74457303130478	10.321123873145648
3	4.0720310248315865	5.367747704904416
4	2.036317993633583	3.5790301105688966
5	1.1568071570171257	2.541503623224683
6	0.7267515902312515	1.9160066103183855
7	0.5013139815737815	1.541940512677632
8	0.35900095009828586	1.2619592952983638
9	0.2590522415488888	1.024447308882109
>10	1.4571164338387466	11.145604428841107
>50	0.08724831811805896	2.781732682322003
>100	0.1487582351246598	14.373479971699037
>500	0.009390535982495266	2.720624246603749
>1k	9.485765085377073E-4	0.6158037900128511
>5k	9.12408988271425E-5	0.2870617555256946
>10k+	2.1506783253617652E-4	6.494652293332058
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	897648	2.698723802793968	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	572801	1.7220911682131388	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA	68630	0.20633189689694625	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	61439	0.1847125952710401	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	61001	0.1833957750635381	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	50399	0.15152151058879781	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA	47473	0.14272467057247165	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG	40432	0.12155633477105246	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	38297	0.1151375878691877	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG	35828	0.10771469039813188	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	6.0128776598264975E-6	0.0	3.0064388299132487E-6	0.0
2	0.0	9.019316489739746E-6	0.0	3.0064388299132487E-6	0.0
3	0.0	1.2025755319652995E-5	0.0	3.0064388299132487E-6	0.0
4	3.0064388299132487E-6	2.104507180939274E-5	0.0	3.0064388299132487E-6	0.0
5	3.0064388299132487E-6	2.104507180939274E-5	0.0	3.0064388299132487E-6	0.0
6	3.0064388299132487E-6	2.405151063930599E-5	0.0	3.0064388299132487E-6	0.0
7	3.0064388299132487E-6	2.405151063930599E-5	0.0	3.0064388299132487E-6	0.0
8	3.0064388299132487E-6	2.405151063930599E-5	0.0	6.0128776598264975E-6	0.0
9	6.0128776598264975E-6	3.0064388299132487E-5	0.0	6.0128776598264975E-6	0.0
10-11	1.052253590469637E-5	3.156760771408911E-5	0.0	1.052253590469637E-5	0.0
12-13	1.9541852394436117E-5	4.209014361878548E-5	0.0	1.2025755319652995E-5	0.0
14-15	3.457404654400236E-5	4.810302127861198E-5	0.0	1.2025755319652995E-5	0.0
16-17	7.215453191791797E-5	5.110946010852523E-5	0.0	1.9541852394436117E-5	0.0
18-19	1.3078008910122633E-4	6.463843484313485E-5	0.0	2.405151063930599E-5	0.0
20-21	1.9541852394436117E-4	7.065131250296135E-5	0.0	2.8561168884175863E-5	0.0
22-23	3.32211490705414E-4	7.666419016278784E-5	0.0	3.0064388299132487E-5	0.0
24-25	4.509658244869873E-4	8.117384840765772E-5	0.0	3.3070827129045736E-5	0.0
26-27	6.644229814108278E-4	8.418028723757096E-5	0.0	3.3070827129045736E-5	0.0
28-29	9.665700838171094E-4	8.718672606748421E-5	0.0	3.6077265958958985E-5	0.0
30-31	0.0012341431396793885	9.019316489739746E-5	0.0	3.6077265958958985E-5	0.0
32-33	0.0014656389295827086	9.319960372731071E-5	0.0	3.6077265958958985E-5	0.0
34-35	0.0018895468046004767	9.770926197218058E-5	0.0	3.6077265958958985E-5	0.0
36-37	0.002496847448242953	1.0071570080209383E-4	0.0	3.758048537391561E-5	0.0
38-39	0.0032785215440203977	1.0221892021705046E-4	0.0	3.9083704788872234E-5	0.0
40-41	0.004463058443006217	1.052253590469637E-4	0.0	3.9083704788872234E-5	1.5032194149566244E-6
42-43	0.006092548288819198	1.112382367067902E-4	0.0	4.509658244869873E-5	3.0064388299132487E-6
44-45	0.008383454677213093	1.1574789495166008E-4	0.0	4.810302127861198E-5	3.0064388299132487E-6
46-47	0.011631911832934359	1.2025755319652995E-4	1.5032194149566244E-6	4.810302127861198E-5	3.0064388299132487E-6
48-49	0.016060396229396576	1.232639920264432E-4	3.0064388299132487E-6	5.110946010852523E-5	6.0128776598264975E-6
50-51	0.022222092611303777	1.292768696862697E-4	6.0128776598264975E-6	5.110946010852523E-5	9.019316489739746E-6
52-53	0.030220723118287975	1.3679296676105283E-4	7.516097074783122E-6	5.411589893843848E-5	1.052253590469637E-5
54-55	0.040668098052236513	1.4430906383583594E-4	1.2025755319652995E-5	5.7122337768351726E-5	1.5032194149566244E-5
56-57	0.0542812530740837	1.4430906383583594E-4	1.352897473460962E-5	6.0128776598264975E-5	1.5032194149566244E-5
58-59	0.07230335063999868	1.4430906383583594E-4	1.8038632979479492E-5	6.0128776598264975E-5	1.5032194149566244E-5
60-61	0.094922293176851	1.473155026657492E-4	1.9541852394436117E-5	6.313521542817822E-5	1.5032194149566244E-5
62-63	0.12300543828707064	1.5032194149566244E-4	2.104507180939274E-5	6.313521542817822E-5	1.5032194149566244E-5
64-65	0.1578846383723092	1.5332838032557569E-4	2.104507180939274E-5	6.313521542817822E-5	1.5032194149566244E-5
66-67	0.20148401428371113	1.5783803857044555E-4	2.104507180939274E-5	6.614165425809147E-5	1.6535413564522868E-5
68-69	0.25559239712507487	1.6084447740035882E-4	2.104507180939274E-5	6.914809308800472E-5	1.9541852394436117E-5
70-71	0.32172202562784663	1.6685735506018532E-4	2.104507180939274E-5	7.065131250296135E-5	2.405151063930599E-5
72-73	0.4006245094995049	1.7136701330505518E-4	2.104507180939274E-5	7.215453191791797E-5	2.405151063930599E-5
74-75	0.494918456960904	1.7888311037983831E-4	2.2548291224349366E-5	7.36577513328746E-5	2.405151063930599E-5
76-77	0.6068767357674585	1.8790242686957803E-4	2.705794946921924E-5	7.816740957774447E-5	2.405151063930599E-5
78-79	0.7363204628087883	1.8940564628453467E-4	3.0064388299132487E-5	7.816740957774447E-5	2.705794946921924E-5
80-81	0.8838133453655024	1.8940564628453467E-4	3.3070827129045736E-5	8.267706782261434E-5	3.3070827129045736E-5
82-83	1.0508616092913872	1.9391530452940453E-4	3.6077265958958985E-5	8.418028723757096E-5	3.3070827129045736E-5
84-85	1.239399897973492	1.9541852394436117E-4	3.6077265958958985E-5	8.418028723757096E-5	3.3070827129045736E-5
86-87	1.449479320871925	1.969217433593178E-4	3.6077265958958985E-5	8.418028723757096E-5	3.758048537391561E-5
88	1.620329226698235	2.0143140160418767E-4	3.6077265958958985E-5	8.418028723757096E-5	4.209014361878548E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	138515	0.0	86.532524	2
ATATCTC	141950	0.0	84.982635	1
TATGGAT	161880	0.0	82.9325	2
GGATCCG	162565	0.0	81.84165	5
ATGGATC	163600	0.0	81.769516	3
ATCTCGA	147305	0.0	80.70735	3
TGGATCC	167600	0.0	79.49726	4
ATATGGA	171445	0.0	78.91375	1
TCTCGAG	151640	0.0	78.25976	4
GATCCGG	171990	0.0	77.42964	6
ATCCGGC	179070	0.0	74.30834	7
CTCGAGG	160065	0.0	74.18043	5
TCCGGCG	185995	0.0	71.79521	8
TCGAGGG	167565	0.0	70.821846	6
CCGGCGC	197945	0.0	67.96185	9
CGAGGGC	182895	0.0	65.00479	7
AGGGCGC	187215	0.0	63.786713	9
GAGGGCG	194150	0.0	61.46711	8
CCGTCGA	184160	0.0	36.07064	16-17
GTCGACT	185055	0.0	35.844128	18-19
>>END_MODULE