##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490975_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33565816
Sequences flagged as poor quality	0
Sequence length	100
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.741139169683823	31.0	31.0	34.0	27.0	34.0
2	31.07143535554148	33.0	31.0	34.0	27.0	34.0
3	31.217349430742278	34.0	31.0	34.0	27.0	34.0
4	34.56527787079569	37.0	35.0	37.0	31.0	37.0
5	34.359182151269614	37.0	35.0	37.0	30.0	37.0
6	34.154554085620916	37.0	35.0	37.0	30.0	37.0
7	34.134107033179234	37.0	35.0	37.0	30.0	37.0
8	33.864338617598335	37.0	35.0	37.0	29.0	37.0
9	35.25838546573693	39.0	35.0	39.0	29.0	39.0
10-11	35.0888744072243	39.0	35.0	39.0	27.5	39.0
12-13	34.796092220728376	39.0	35.0	39.0	26.0	39.0
14-15	35.60459829130923	40.0	36.0	41.0	25.5	41.0
16-17	35.49818881209383	39.5	35.5	41.0	25.0	41.0
18-19	35.41611581854587	39.5	35.5	41.0	24.0	41.0
20-21	34.350623428907554	38.5	33.5	40.5	19.0	41.0
22-23	34.17074737584214	39.0	33.5	40.5	17.5	41.0
24-25	34.74294806358945	39.0	34.0	41.0	22.5	41.0
26-27	35.2172117311255	39.0	35.0	41.0	25.5	41.0
28-29	35.302647416645556	39.0	35.0	41.0	26.0	41.0
30-31	34.825806916179246	39.0	35.0	40.5	24.5	41.0
32-33	33.38864128910198	36.0	34.0	40.0	21.0	41.0
34-35	32.868395676720624	35.0	33.0	40.0	17.0	41.0
36-37	32.54466422028888	35.0	33.0	40.0	13.5	41.0
38-39	32.20211896531876	35.0	33.0	40.0	8.5	41.0
40-41	31.844004388274072	35.0	33.0	40.0	2.0	41.0
42-43	31.41048467285884	35.0	33.0	40.0	2.0	41.0
44-45	30.899544897106033	35.0	31.5	40.0	2.0	41.0
46-47	29.986114816931607	35.0	29.5	39.0	2.0	41.0
48-49	28.321492735347178	35.0	21.5	39.0	2.0	41.0
50-51	26.86792206988205	34.5	10.5	39.0	2.0	41.0
52-53	26.298990720201765	34.0	7.0	39.0	2.0	41.0
54-55	25.8944836615919	34.0	6.0	39.0	2.0	41.0
56-57	25.484980701794946	33.5	2.0	38.0	2.0	41.0
58-59	25.178098217543706	33.0	2.0	38.0	2.0	41.0
60-61	24.856894153861774	33.0	2.0	38.0	2.0	40.0
62-63	24.496131749038963	33.0	2.0	37.0	2.0	40.0
64-65	24.08848156708003	33.0	2.0	36.5	2.0	40.0
66-67	23.673749939521805	32.5	2.0	36.0	2.0	40.0
68-69	23.181593931754854	31.5	2.0	35.0	2.0	39.0
70-71	22.68696679383573	31.0	2.0	35.0	2.0	39.0
72-73	22.15371264920239	31.0	2.0	35.0	2.0	38.5
74-75	21.57011024251578	30.0	2.0	35.0	2.0	37.5
76-77	20.955941127723513	29.0	2.0	35.0	2.0	37.0
78-79	20.578383883174475	29.0	2.0	35.0	2.0	36.5
80-81	19.934408938546287	28.0	2.0	35.0	2.0	36.0
82-83	19.217317731825737	26.5	2.0	34.5	2.0	35.5
84-85	18.584572724226337	24.5	2.0	34.0	2.0	35.0
86-87	17.86358091219948	21.5	2.0	34.0	2.0	35.0
88-89	17.099016466633792	12.0	2.0	34.0	2.0	35.0
90-91	16.32289709566423	2.0	2.0	33.0	2.0	35.0
92-93	15.596729556641794	2.0	2.0	33.0	2.0	35.0
94-95	14.810278483919474	2.0	2.0	33.0	2.0	35.0
96-97	14.093950106858717	2.0	2.0	32.0	2.0	35.0
98-99	13.481863095477852	2.0	2.0	31.5	2.0	35.0
100	12.873832889985454	2.0	2.0	31.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.07273452936386704
1101	2	0.09060306433994825
1101	3	0.1348724185821979
1101	4	0.16054997123819703
1101	5	0.16148194455083598
1101	6	0.181139402779408
1101	7	0.17410067748022584
1101	8	0.1423806487624404
1101	9	0.14654537689479952
1101	10-11	0.065595102584183
1101	12-13	-0.1714795867797818
1101	14-15	-0.3081678528039191
1101	16-17	-0.3623687477864692
1101	18-19	-0.4041641118472299
1101	20-21	-0.600036191639056
1101	22-23	-0.5793082802507925
1101	24-25	-0.49300374886559695
1101	26-27	-0.37277148127184745
1101	28-29	-0.1988572737652845
1101	30-31	-0.13307852689504074
1101	32-33	-0.0630610751819134
1101	34-35	-0.07595313420648608
1101	36-37	-0.062141548977116656
1101	38-39	-0.10632723701102265
1101	40-41	-0.09152139088849154
1101	42-43	-0.09807273849515497
1101	44-45	-0.12571504388747456
1101	46-47	-0.11593615740016006
1101	48-49	-0.0721867575167181
1101	50-51	-0.13553240089493457
1101	52-53	-0.23091228801401797
1101	54-55	-0.24993775314129252
1101	56-57	-0.2625960563553065
1101	58-59	-0.302238176750798
1101	60-61	-0.2812576695226561
1101	62-63	-0.39861883436157086
1101	64-65	-0.3780973841609949
1101	66-67	-0.4123223364249249
1101	68-69	-0.4330939521193038
1101	70-71	-0.4688938691830664
1101	72-73	-0.5182886882974493
1101	74-75	-0.5225457909201587
1101	76-77	-0.5737871767166602
1101	78-79	-0.6569737399932158
1101	80-81	-0.6142052556182023
1101	82-83	-0.5610331692342072
1101	84-85	-0.6085275756293633
1101	86-87	-0.711897991027751
1101	88-89	-0.8044542186331007
1101	90-91	-0.8734789709982209
1101	92-93	-0.8988763079355486
1101	94-95	-0.9225409494233432
1101	96-97	-1.1011489337168516
1101	98-99	-1.1048341692268924
1101	100	-1.0150657374349024
1102	1	0.09636770422893548
1102	2	0.13274961040896116
1102	3	0.15289057776072568
1102	4	0.17734177277220908
1102	5	0.14965303927223772
1102	6	0.1956294655154025
1102	7	0.16644970114380442
1102	8	0.19331207394452576
1102	9	0.19471588175991172
1102	10-11	0.1512998698197663
1102	12-13	0.013051112341223359
1102	14-15	-0.018819474173511708
1102	16-17	-0.03669000169123393
1102	18-19	-0.024133833496918555
1102	20-21	-0.1665958011366726
1102	22-23	-0.1433252573581072
1102	24-25	-0.10536688566573815
1102	26-27	-0.010041575902349109
1102	28-29	0.07834801456633045
1102	30-31	0.09222317725441798
1102	32-33	0.11190489574243401
1102	34-35	0.1541863513010142
1102	36-37	0.13197726753518424
1102	38-39	0.14253417745526775
1102	40-41	0.13839448416304734
1102	42-43	0.14226346988171557
1102	44-45	0.1230101744758656
1102	46-47	0.1071443675796857
1102	48-49	0.14948189304632464
1102	50-51	0.1968465515352058
1102	52-53	0.12269011832482235
1102	54-55	0.07065881046227318
1102	56-57	0.0822174388839727
1102	58-59	0.047331031627756204
1102	60-61	0.03952305971568748
1102	62-63	-0.007111607215861682
1102	64-65	0.007148131456677476
1102	66-67	-0.037066732585021356
1102	68-69	-0.05420017646333264
1102	70-71	-0.0992010796214835
1102	72-73	-0.1024141081825185
1102	74-75	-0.08575333720861167
1102	76-77	-0.12363653654702844
1102	78-79	-0.18676583405067504
1102	80-81	-0.20834830199370913
1102	82-83	-0.16554521587806548
1102	84-85	-0.25748720449063356
1102	86-87	-0.33416330749357925
1102	88-89	-0.3912897269814941
1102	90-91	-0.44272707680231704
1102	92-93	-0.473806856033292
1102	94-95	-0.5244701697225018
1102	96-97	-0.6209556234024731
1102	98-99	-0.6607326778210574
1102	100	-0.6183921408390916
1103	1	-0.014295737452258095
1103	2	-0.035862343654375906
1103	3	-0.027670110831298445
1103	4	-0.033180717530605364
1103	5	0.017531737235472633
1103	6	0.04269387785615919
1103	7	0.025247144345414085
1103	8	0.06012939120362404
1103	9	-0.015400907467864045
1103	10-11	-0.016733662491141388
1103	12-13	-0.11519843926191697
1103	14-15	-0.127477853235483
1103	16-17	-0.17594329688166965
1103	18-19	-0.17542180751728864
1103	20-21	-0.22595343093032483
1103	22-23	-0.16740681411475578
1103	24-25	-0.16149431089279886
1103	26-27	-0.16825515684406867
1103	28-29	-0.08077417413650778
1103	30-31	-0.13109640751296325
1103	32-33	-0.15530802311707248
1103	34-35	-0.10886557219440363
1103	36-37	-0.10493253966316018
1103	38-39	-0.13968331562522707
1103	40-41	-0.10385491077288123
1103	42-43	-0.04004826019987462
1103	44-45	-0.11218732540369203
1103	46-47	-0.16438037667187544
1103	48-49	-0.21707955340957596
1103	50-51	-0.1351857458069432
1103	52-53	-0.07667046010286072
1103	54-55	-0.11606786033035732
1103	56-57	-0.08962188662395931
1103	58-59	-0.15910464652026235
1103	60-61	-0.17451763552114485
1103	62-63	-0.24917771103424258
1103	64-65	-0.26101781054341444
1103	66-67	-0.2272184115097282
1103	68-69	-0.22732754010955958
1103	70-71	-0.2131051940507369
1103	72-73	-0.27243904304043554
1103	74-75	-0.2545980151595231
1103	76-77	-0.21542305959069097
1103	78-79	-0.24174218753709553
1103	80-81	-0.18097926275980214
1103	82-83	-0.1366517638105016
1103	84-85	-0.1747967813688085
1103	86-87	-0.28684139024430877
1103	88-89	-0.20889149199850365
1103	90-91	-0.24417466239691166
1103	92-93	-0.2491831046496511
1103	94-95	-0.3401871475212843
1103	96-97	-0.42488283250140846
1103	98-99	-0.4076486235565646
1103	100	-0.3973929299937424
1104	1	-0.02072317541983182
1104	2	0.003915226184734166
1104	3	-0.02489485574461625
1104	4	0.036764657520080846
1104	5	-0.08716797214648153
1104	6	-0.037013241310383194
1104	7	-0.0116441317259941
1104	8	-0.06865809392601108
1104	9	-0.07740666553991815
1104	10-11	-0.11842294583851043
1104	12-13	-0.08824638688777497
1104	14-15	-0.023290571387455827
1104	16-17	-0.05143025873048401
1104	18-19	-0.004240917539831912
1104	20-21	-0.06560973571377104
1104	22-23	-0.059431960126353545
1104	24-25	-0.1883369949039917
1104	26-27	-0.05307497567811481
1104	28-29	0.014019766188773986
1104	30-31	-0.022122207917426806
1104	32-33	0.025823769453282353
1104	34-35	0.0675336207523003
1104	36-37	0.028803984588257947
1104	38-39	0.07701313297591383
1104	40-41	0.02922850542302413
1104	42-43	0.005172065381863433
1104	44-45	0.06556456756820594
1104	46-47	-0.007888889608597793
1104	48-49	0.1273197477907395
1104	50-51	0.02133776121045372
1104	52-53	0.06194654871143257
1104	54-55	0.1611747221819826
1104	56-57	0.11844197845572779
1104	58-59	0.08491973168546352
1104	60-61	0.11874741810874312
1104	62-63	0.06839346577271499
1104	64-65	0.1525874377688936
1104	66-67	0.0839109246193992
1104	68-69	0.10622094275307603
1104	70-71	0.20126329071799276
1104	72-73	0.12902484644838097
1104	74-75	0.2755180233426451
1104	76-77	0.27469728340328814
1104	78-79	0.18777304357309887
1104	80-81	0.23653596109409136
1104	82-83	0.16715888833528325
1104	84-85	0.24739185084274595
1104	86-87	0.18395647357020906
1104	88-89	0.23614001306097165
1104	90-91	0.17874008204687541
1104	92-93	0.19231898482601473
1104	94-95	0.18428596891118332
1104	96-97	0.07611361148654261
1104	98-99	0.03849505986976531
1104	100	0.076834116989696
1105	1	0.012688819446918131
1105	2	0.00360919966155393
1105	3	0.005626036505368859
1105	4	0.04533434138703285
1105	5	-0.004902683464159452
1105	6	0.03947476585636167
1105	7	-0.0034372030215124028
1105	8	-0.005889879252897856
1105	9	0.06281418597384913
1105	10-11	0.00927800990218941
1105	12-13	0.09410982744165608
1105	14-15	0.08476952577713348
1105	16-17	0.0499248942749162
1105	18-19	0.0670335615745401
1105	20-21	0.03473736346723655
1105	22-23	0.03128907022226457
1105	24-25	0.04608716993397621
1105	26-27	0.0691486645790178
1105	28-29	0.01081287135296094
1105	30-31	0.06060760299718737
1105	32-33	0.07542630045816168
1105	34-35	0.009309360050139048
1105	36-37	0.015944782359419207
1105	38-39	0.045784170005831015
1105	40-41	0.12730616587173316
1105	42-43	0.13874371877049896
1105	44-45	0.16421069067351368
1105	46-47	0.1454134812436827
1105	48-49	0.21579913085646396
1105	50-51	0.32457029500731593
1105	52-53	0.2838623445049677
1105	54-55	0.2671003605346378
1105	56-57	0.13069471648576325
1105	58-59	0.30956098540753274
1105	60-61	0.29364390087033954
1105	62-63	0.2906331748503739
1105	64-65	0.2836736729296945
1105	66-67	0.3249543633037426
1105	68-69	0.3621913801792722
1105	70-71	0.37823105429217563
1105	72-73	0.29665732250157717
1105	74-75	0.3059050169174249
1105	76-77	0.362014241014915
1105	78-79	0.3698464034804374
1105	80-81	0.3468008954384061
1105	82-83	0.38716045115702613
1105	84-85	0.4659146351924264
1105	86-87	0.4555412702162407
1105	88-89	0.5050381270921314
1105	90-91	0.48500775600773594
1105	92-93	0.4636793120464606
1105	94-95	0.4330084111969743
1105	96-97	0.39096871811738865
1105	98-99	0.40439016689434304
1105	100	0.42495933101415595
1106	1	-0.19271363244261153
1106	2	-0.14506301984458858
1106	3	-0.1339050304215128
1106	4	-0.07226381779605617
1106	5	-0.09297219483885755
1106	6	-0.06375875959775357
1106	7	-0.12677453526772808
1106	8	-0.12750632385098726
1106	9	-0.18859935117870918
1106	10-11	-0.08395965429440366
1106	12-13	-0.002850816980149773
1106	14-15	0.05252837678065703
1106	16-17	0.039336926034678754
1106	18-19	0.0650479344644097
1106	20-21	0.10172746159838653
1106	22-23	0.09315578315985817
1106	24-25	0.06679133102171875
1106	26-27	0.016288744184151938
1106	28-29	-0.053793456415945684
1106	30-31	-0.08767358053876961
1106	32-33	-0.08054010791246924
1106	34-35	0.008075206161336723
1106	36-37	-0.055439570818940354
1106	38-39	-0.012120375801764283
1106	40-41	-0.014048628598448687
1106	42-43	0.008718379076846361
1106	44-45	0.02064890779467987
1106	46-47	0.02717820068166077
1106	48-49	0.005650795832540467
1106	50-51	0.12489258017577853
1106	52-53	0.11571645660410468
1106	54-55	0.09423012945399378
1106	56-57	0.14751363754366764
1106	58-59	0.17839695401003297
1106	60-61	0.19730950254475843
1106	62-63	0.09997922989278152
1106	64-65	0.15683746419620093
1106	66-67	0.13638308105732122
1106	68-69	0.1737157652300816
1106	70-71	0.23977169033348034
1106	72-73	0.2033679721779862
1106	74-75	0.2616142826115109
1106	76-77	0.31934317119989686
1106	78-79	0.3129248136753091
1106	80-81	0.3281743199082072
1106	82-83	0.39865876982022286
1106	84-85	0.39372544311114055
1106	86-87	0.36622974670329356
1106	88-89	0.3742376977731787
1106	90-91	0.40142608502957344
1106	92-93	0.30476770102978357
1106	94-95	0.33542628008349773
1106	96-97	0.24092427450312393
1106	98-99	0.23721411164271267
1106	100	0.24448402317818818
1107	1	-0.21297860902878085
1107	2	-0.2020825480756443
1107	3	-0.20687001738006572
1107	4	-0.15696538898254886
1107	5	-0.20886733128269697
1107	6	-0.3291504122042497
1107	7	-0.254807012026113
1107	8	-0.1980023646848963
1107	9	-0.20979664060629943
1107	10-11	-0.16746241906597703
1107	12-13	-0.04664425934748806
1107	14-15	0.0036583593886518884
1107	16-17	0.042762545232264415
1107	18-19	0.11237548664659869
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2207	100	0.6125438490925461
2208	1	-0.8067853578401447
2208	2	-0.5935231271094281
2208	3	-0.5525358062326831
2208	4	-0.45790444635002814
2208	5	-0.6875014374564543
2208	6	-0.5981750539298432
2208	7	-0.5883954094103601
2208	8	-0.5012321651601894
2208	9	-0.6095776108964728
2208	10-11	-0.5307992515285846
2208	12-13	-0.42603388063552927
2208	14-15	-0.35084261793579685
2208	16-17	-0.32405039596294216
2208	18-19	-0.3152085001599758
2208	20-21	-0.19597741578569838
2208	22-23	-0.19292754365601184
2208	24-25	-0.4260527136132666
2208	26-27	-0.4683442134796536
2208	28-29	-0.6236714673106363
2208	30-31	-0.7437587779624621
2208	32-33	-0.7245022121553575
2208	34-35	-0.6889740726556255
2208	36-37	-0.7658240143279755
2208	38-39	-0.7721814647465948
2208	40-41	-0.7560308219535905
2208	42-43	-0.8294422576456242
2208	44-45	-0.8507509492537295
2208	46-47	-0.8692501442436829
2208	48-49	-0.7652431546515182
2208	50-51	-0.7358942076120556
2208	52-53	-0.626029896179503
2208	54-55	-0.5835469569906628
2208	56-57	-0.5885983094625153
2208	58-59	-1.1365041145784822
2208	60-61	-0.8208758439328925
2208	62-63	-0.7470656892885472
2208	64-65	-0.6903810968371218
2208	66-67	-0.620813027470831
2208	68-69	-0.6375359743815778
2208	70-71	-0.5273865781482492
2208	72-73	-0.47310390125572965
2208	74-75	-0.5179811643170531
2208	76-77	-0.5015839019021051
2208	78-79	-0.483155970730202
2208	80-81	-0.43865157929891296
2208	82-83	-0.39716842008995457
2208	84-85	-0.40654585374622343
2208	86-87	-0.39069921983470124
2208	88-89	-0.3330293392563526
2208	90-91	-1.12982694629288
2208	92-93	-0.7230931483862992
2208	94-95	-0.43569282758962835
2208	96-97	-0.06830921236983123
2208	98-99	0.07481954561831827
2208	100	0.04673179986320619
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	688155.0
3	295946.0
4	303516.0
5	263237.0
6	171180.0
7	200822.0
8	285748.0
9	313730.0
10	271213.0
11	238373.0
12	260836.0
13	323720.0
14	441932.0
15	581658.0
16	717930.0
17	917594.0
18	1468877.0
19	1188229.0
20	768402.0
21	652785.0
22	662084.0
23	710508.0
24	785390.0
25	871960.0
26	961949.0
27	1030871.0
28	1088223.0
29	1135167.0
30	1184279.0
31	1240103.0
32	1331714.0
33	1472420.0
34	1508361.0
35	1544947.0
36	1867680.0
37	2552560.0
38	2661978.0
39	601180.0
40	559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.655595747120993	50.380622172356546	19.568324502340275	15.395457578182183
2	16.15359934113804	19.868084501479245	49.298313443130944	14.680002714251778
3	17.36553795312789	48.035289072651196	17.840002733220505	16.759170241000408
4	18.48542208943407	17.142671502677132	47.67901107269554	16.69289533519326
5	34.7054519880338	17.492608797587007	16.39104812084147	31.410891093537725
6	35.40604578311824	17.483751018667483	29.93938051128427	17.17082268693001
7	19.48278543352437	33.83981726427268	15.714063721103535	30.963333581099413
8	33.94630973527862	17.32136001763764	31.115427077749924	17.61690316933382
9	19.802308928191266	31.294953683115427	15.472005308941513	33.43073207975179
10-11	40.97206299441306	16.928017337615465	15.820840845322003	26.279078822649478
12-13	33.13952306725011	17.1731899917755	16.316400285828024	33.37088665514637
14-15	31.964348046693598	17.970586988026145	17.766003223357657	32.2990617419226
16-17	30.807759413775088	17.914622047220746	18.633143294318497	32.644475244685665
18-19	29.663266138291846	18.15451486476819	21.396958646781737	30.78526035015823
20-21	21.301211792130353	24.54198685988426	31.329758732312012	22.82704261567337
22-23	20.417419719773203	23.95784835680179	33.2590762648558	22.36565565856921
24-25	12.789179305190665	16.751659582036314	42.5799009397439	27.879260173029124
26-27	12.646288425875413	16.648275642791504	58.1253110893054	12.580124842027681
28-29	12.609603413700018	16.67319081463338	58.22685622389487	12.49034954777173
30-31	12.63075006980613	16.648668771327074	58.20262491899905	12.517956239867756
32-33	12.63109961905326	16.66514298301065	58.14044431926533	12.563313078670769
34-35	12.727079775776003	16.745578040913692	58.04424473984098	12.48309744346933
36-37	12.702098985047435	16.86188553383996	57.868712101679556	12.567303379433048
38-39	12.812984950856327	16.954145663428566	57.58519907487811	12.647670310836997
40-41	12.911631104223323	17.193733364688054	57.11729155520834	12.777343975880287
42-43	13.090207179308186	17.565817616478647	56.34722696314135	12.996748241071817
44-45	13.296257420514587	18.148777836252048	55.34257381116723	13.212390932066132
46-47	13.692511874996068	19.044997210414884	53.7832069926395	13.47928392194955
48-49	14.351225274459772	21.089171295847837	50.327023110227145	14.232580319465244
50-51	15.306428937186167	24.23170566314981	45.37944175646304	15.08242364320099
52-53	15.903616396655943	26.793664274807856	41.6865983267791	15.616121001757108
54-55	16.158746123031666	28.976064935234646	38.84482782589872	16.02036111583497
56-57	16.273041219741923	31.102989620974604	36.41536866145286	16.208600497830613
58-59	16.364231250075427	32.84267270503492	34.53999383469014	16.253102210199522
60-61	16.326685184308698	34.35590053449806	32.932252332571416	16.385161948621818
62-63	16.36674763956503	35.70930441469645	31.58228919212923	16.34165875360929
64-65	16.426666081906777	36.87275097912149	30.34144977181925	16.35913316715248
66-67	16.529561537847197	37.796728840427754	29.2884538259539	16.385255795771155
68-69	16.68256463063525	38.752400430667045	28.12359385628599	16.44144108241171
70-71	16.882972072586195	39.557447284771406	27.14551731656274	16.414063326079656
72-73	17.047974605152767	40.294260407017134	26.03375297282528	16.624012015004812
74-75	17.294767449023922	40.85494387775846	25.19284052101092	16.657448152206694
76-77	17.265360638380166	41.56137520830828	24.254947399813272	16.918316753498285
78-79	17.5484255669675	41.69377913183913	23.614822329210988	17.14297297198239
80-81	17.834678933358784	41.7793444393462	23.00144812521916	17.38452850207586
82-83	18.122819713273007	41.65179776131443	22.355281686515934	17.87010083889663
84-85	18.496793516197677	41.29474168122624	21.86588549030695	18.342579312269134
86-87	18.880779882990435	40.8204645234903	21.35542350205206	18.9433320914672
88-89	19.32646872018204	40.21090095577952	20.873124700241917	19.589505623796523
90-91	19.64134497029347	39.524911296288955	20.443353977981157	20.390389755436416
92-93	20.1103009438474	38.847626146704535	20.196334439646535	20.84573846980154
94-95	20.351833331247622	38.29671710356071	19.82203212725527	21.529417437936406
96-97	20.572491643425757	37.796431731393085	19.550225604619218	22.08085102056194
98-99	21.176517814922075	37.12797268461047	19.368900069148868	22.326609431318587
100	22.386973618379944	36.60219132733537	18.294995674105643	22.715839380179048
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	33289.0
1	21341.0
2	8423.5
3	7394.5
4	7470.5
5	7570.5
6	7795.5
7	8611.0
8	10367.0
9	13072.0
10	18579.0
11	26912.5
12	33637.0
13	59496.5
14	133911.5
15	229765.5
16	1301523.0
17	1599361.0
18	764161.5
19	615892.5
20	545760.5
21	501683.5
22	480956.5
23	477622.0
24	486487.0
25	505174.5
26	529377.5
27	557862.0
28	588951.0
29	615325.5
30	632088.0
31	641092.0
32	645931.5
33	646595.5
34	646164.5
35	659321.0
36	687968.5
37	731545.5
38	776334.5
39	806379.5
40	832773.0
41	864995.0
42	912341.5
43	955086.5
44	982910.5
45	988353.5
46	966136.5
47	931772.0
48	889706.0
49	856234.0
50	815791.0
51	758583.0
52	692195.5
53	619779.5
54	559092.5
55	506692.0
56	451456.5
57	400342.0
58	357130.5
59	317073.0
60	279912.0
61	244174.0
62	210997.5
63	181900.0
64	156494.5
65	134377.0
66	114520.5
67	96937.0
68	81367.5
69	67629.5
70	55790.0
71	45774.5
72	37400.5
73	30427.5
74	24670.5
75	19923.5
76	16005.0
77	12828.5
78	10201.5
79	8031.5
80	6253.0
81	4801.5
82	3600.0
83	2613.0
84	1850.0
85	1339.0
86	936.5
87	664.5
88	467.0
89	311.0
90	220.0
91	144.5
92	90.0
93	63.0
94	41.0
95	27.0
96	18.5
97	9.0
98	5.5
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17019398545234235
2	0.07252616769394196
3	0.06842675893831986
4	0.08746100497005643
5	0.09542446398442987
6	0.03993646393104222
7	0.03772290237186547
8	0.0307157734523719
9	0.03981729507186716
10-11	0.03560616551076845
12-13	0.029714755035301393
14-15	0.030158659035728494
16-17	0.030276338284163866
18-19	0.032132393265815434
20-21	0.03144866193629853
22-23	0.028716715839710256
24-25	0.030024594069156547
26-27	0.021824286947172682
28-29	0.021317819295678678
30-31	0.020257216449020635
32-33	0.020240830730884063
34-35	0.01729140146630131
36-37	0.02043299051630385
38-39	0.024703704506990085
40-41	0.023450941874912264
42-43	0.031097113801732093
44-45	0.03186873216489061
46-47	0.031719771090921785
48-49	0.027991275409482076
50-51	0.027536944133877153
52-53	0.027642706496395022
54-55	0.029185943222712056
56-57	0.02567790993074621
58-59	0.021095867295465125
60-61	0.011224216923551031
62-63	0.009372630774118526
64-65	0.006502150878739251
66-67	0.008051346048015039
68-69	0.013080271905202603
70-71	0.019408138327398326
72-73	0.017218410540056586
74-75	0.016792381868505745
76-77	0.0219866545177987
78-79	0.019451337038849284
80-81	0.012353341864234733
82-83	0.014915472336498536
84-85	0.017881287319217863
86-87	0.022190731189135993
88-89	0.02546489559497079
90-91	0.030225691519014462
92-93	0.08430303020191733
94-95	0.13135834385792974
96-97	0.08653744631144972
98-99	0.0684222901061008
100	0.07381021215155324
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.3565816E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.080797015293314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.90698180471247	29.552691846311536
2	9.730815366363885	7.021911480541447
3	3.2184031770576063	3.4836765523437183
4	1.4480033469800921	2.0898045935941614
5	0.8339945317585372	1.5045593706122184
6	0.5469722451175022	1.1841116729450314
7	0.3707948211464042	0.936500087327378
8	0.2749881693011762	0.793743385453028
9	0.2025224374456384	0.6576453860866665
>10	1.1877441675706277	7.7829554687933635
>50	0.12226879644102494	3.148362519850472
>100	0.14908572263183975	10.465177892940584
>500	0.003535191750729655	0.855554150675259
>1k	0.0029457960473035726	2.393148027334877
>5k	4.3532120969809045E-4	1.0485896953997875
>10k+	5.09104465519038E-4	27.08156786979043
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	3168558	9.439836052250302	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	3145485	9.371096475056646	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	332440	0.9904123886039297	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	283718	0.8452587596857469	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA	196576	0.5856434415299184	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	171270	0.5102512627728163	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA	137989	0.4110997927176864	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	131724	0.39243497014939244	No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	125456	0.37376120991666045	No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	117685	0.3506096798004255	No Hit
ATATGGATCCGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	115487	0.34406135098875595	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	112225	0.3343431305230297	No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT	104754	0.3120853668506078	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	99391	0.2961078020567115	No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97790	0.29133806846822974	No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91922	0.27385599682724826	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	85954	0.2560760030383292	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85688	0.2552835301248151	No Hit
TCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84202	0.25085640700646156	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70503	0.21004405196048265	No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT	69961	0.2084293139186606	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG	69697	0.20764279944810518	No Hit
ATATGGATCCGGCGCGCCGGCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	63369	0.18879028592661057	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC	63152	0.18814379486558586	No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62640	0.18661843346814508	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA	60488	0.18020714884452682	No Hit
ATATGGATCCGGCGCGCCGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTTT	55600	0.1656447142533344	No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55087	0.16411637363441425	No Hit
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54922	0.16362480209031713	No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54529	0.16245396804892215	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA	52268	0.15571794828405186	No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44675	0.13309671959114594	No Hit
GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43463	0.1294859031581416	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG	41836	0.124638709811196	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC	40970	0.12205870401005593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.979221479376518E-6	0.0	0.0	0.0	2.979221479376518E-6
2	2.979221479376518E-6	2.979221479376518E-6	0.0	0.0	2.979221479376518E-6
3	2.979221479376518E-6	2.979221479376518E-6	2.979221479376518E-6	0.0	2.979221479376518E-6
4	2.979221479376518E-6	5.958442958753036E-6	2.979221479376518E-6	0.0	2.979221479376518E-6
5	2.979221479376518E-6	5.958442958753036E-6	2.979221479376518E-6	2.979221479376518E-6	2.979221479376518E-6
6	2.979221479376518E-6	5.958442958753036E-6	2.979221479376518E-6	2.979221479376518E-6	5.958442958753036E-6
7	2.979221479376518E-6	5.958442958753036E-6	2.979221479376518E-6	2.979221479376518E-6	5.958442958753036E-6
8	2.979221479376518E-6	8.937664438129554E-6	2.979221479376518E-6	2.979221479376518E-6	5.958442958753036E-6
9	5.958442958753036E-6	8.937664438129554E-6	2.979221479376518E-6	2.979221479376518E-6	5.958442958753036E-6
10-11	5.958442958753036E-6	8.937664438129554E-6	2.979221479376518E-6	7.448053698441295E-6	5.958442958753036E-6
12-13	1.1916885917506072E-5	1.787532887625911E-5	2.979221479376518E-6	8.937664438129554E-6	8.937664438129554E-6
14-15	3.575065775251822E-5	2.0854550355635628E-5	2.979221479376518E-6	1.787532887625911E-5	1.489610739688259E-5
16-17	1.2959613435287854E-4	2.3833771835012143E-5	2.979221479376518E-6	1.787532887625911E-5	1.787532887625911E-5
18-19	4.796546581796194E-4	2.6812993314388662E-5	2.979221479376518E-6	1.787532887625911E-5	1.787532887625911E-5
20-21	0.0015179133437423359	2.979221479376518E-5	2.979221479376518E-6	1.787532887625911E-5	1.787532887625911E-5
22-23	0.005885452032508311	2.979221479376518E-5	2.979221479376518E-6	1.936493961594737E-5	1.787532887625911E-5
24-25	0.013105595287777303	2.979221479376518E-5	2.979221479376518E-6	2.0854550355635628E-5	1.787532887625911E-5
26-27	0.023245375592835283	3.872987923189474E-5	2.979221479376518E-6	2.3833771835012143E-5	1.787532887625911E-5
28-29	0.06479210873348051	4.1709100711271255E-5	4.468832219064776E-6	2.830260405407692E-5	1.787532887625911E-5
30-31	0.09819365034951036	4.3198711450959515E-5	5.958442958753036E-6	3.128182553345344E-5	1.787532887625911E-5
32-33	0.09879992192056347	4.4688322190647774E-5	5.958442958753036E-6	3.27714362731417E-5	1.787532887625911E-5
34-35	0.099619207827392	5.0646765149400805E-5	5.958442958753036E-6	3.27714362731417E-5	1.787532887625911E-5
36-37	0.10078706264730761	5.0646765149400805E-5	5.958442958753036E-6	3.426104701282996E-5	1.787532887625911E-5
38-39	0.10225432922590054	5.3625986628777324E-5	5.958442958753036E-6	4.0219489971582996E-5	1.787532887625911E-5
40-41	0.10370669969709659	5.660520810815384E-5	5.958442958753036E-6	4.1709100711271255E-5	1.787532887625911E-5
42-43	0.10533633384631555	5.660520810815384E-5	5.958442958753036E-6	4.1709100711271255E-5	1.787532887625911E-5
44-45	0.10712237712320177	5.958442958753036E-5	5.958442958753036E-6	4.7667543670024286E-5	1.787532887625911E-5
46-47	0.10930018802462602	5.958442958753036E-5	5.958442958753036E-6	5.0646765149400805E-5	1.936493961594737E-5
48-49	0.1118146509532198	6.405326180659514E-5	5.958442958753036E-6	5.0646765149400805E-5	2.0854550355635628E-5
50-51	0.11506647119795926	6.703248328597166E-5	5.958442958753036E-6	5.0646765149400805E-5	2.3833771835012143E-5
52-53	0.11956062679959874	6.852209402565992E-5	5.958442958753036E-6	5.0646765149400805E-5	2.6812993314388662E-5
54-55	0.12525391904668726	7.29909262447247E-5	5.958442958753036E-6	5.0646765149400805E-5	2.6812993314388662E-5
56-57	0.1324040505971909	7.448053698441295E-5	5.958442958753036E-6	5.0646765149400805E-5	2.6812993314388662E-5
58-59	0.1412746825520345	8.043897994316599E-5	5.958442958753036E-6	5.0646765149400805E-5	2.6812993314388662E-5
60-61	0.1524259085493408	8.341820142254251E-5	5.958442958753036E-6	5.3625986628777324E-5	2.6812993314388662E-5
62-63	0.1662137455558953	8.639742290191903E-5	5.958442958753036E-6	5.660520810815384E-5	2.830260405407692E-5
64-65	0.18318041188094458	8.639742290191903E-5	5.958442958753036E-6	5.958442958753036E-5	2.979221479376518E-5
66-67	0.2040722025050724	8.937664438129555E-5	8.937664438129554E-6	5.958442958753036E-5	2.979221479376518E-5
68-69	0.2298797085701715	9.235586586067205E-5	8.937664438129554E-6	5.958442958753036E-5	2.979221479376518E-5
70-71	0.26070422360654066	9.682469807973683E-5	8.937664438129554E-6	6.703248328597166E-5	3.27714362731417E-5
72-73	0.29700156850052445	1.0278314103848987E-4	8.937664438129554E-6	7.150131550503644E-5	3.27714362731417E-5
74-75	0.3403209384213987	1.0725197325755465E-4	8.937664438129554E-6	7.150131550503644E-5	3.27714362731417E-5
76-77	0.3927209754114126	1.0725197325755465E-4	8.937664438129554E-6	7.894936920347773E-5	3.27714362731417E-5
78-79	0.4538665170541363	1.1023119473693117E-4	8.937664438129554E-6	8.490781216223077E-5	3.426104701282996E-5
80-81	0.5231170307315038	1.1023119473693117E-4	8.937664438129554E-6	9.384547660036031E-5	3.575065775251822E-5
82-83	0.6021200259216102	1.1321041621630768E-4	8.937664438129554E-6	1.0427275177817813E-4	3.724026849220648E-5
84-85	0.6910497870809993	1.1916885917506072E-4	8.937664438129554E-6	1.087415839972429E-4	4.3198711450959515E-5
86-87	0.7891466127324299	1.236376913941255E-4	8.937664438129554E-6	1.1023119473693117E-4	4.617793293033603E-5
88	0.8697002927025519	1.2512730213381376E-4	8.937664438129554E-6	1.1321041621630768E-4	4.7667543670024286E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	491015	0.0	92.325676	1
ATATGGA	540450	0.0	91.035	1
TATCTCG	500630	0.0	89.897934	2
TATGGAT	548360	0.0	89.3562	2
ATGGATC	559515	0.0	87.31615	3
TGGATCC	572515	0.0	84.95321	4
GGATCCG	576700	0.0	83.95596	5
ATCTCGA	546020	0.0	81.834335	3
GATCCGG	608480	0.0	79.467834	6
TCTCGAG	560700	0.0	79.15762	4
ATCCGGC	614655	0.0	77.03551	7
CTCGAGG	584420	0.0	75.75078	5
TCCGGCG	632385	0.0	74.35647	8
TCGAGGG	604415	0.0	73.11823	6
CCGGCGC	661490	0.0	70.79712	9
CGAGGGC	629850	0.0	69.69213	7
GAGGGCG	664290	0.0	65.9518	8
AGGGCGC	669285	0.0	65.02001	9
TATCGCG	1940	0.0	46.3025	2
CTCGACG	3865	0.0	44.16578	5
>>END_MODULE