##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490974_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30282390
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.007589460409168	31.0	31.0	34.0	28.0	34.0
2	31.28955769343173	33.0	31.0	34.0	28.0	34.0
3	31.41228492863344	34.0	31.0	34.0	28.0	34.0
4	34.7703992980739	37.0	35.0	37.0	32.0	37.0
5	34.58419404809198	37.0	35.0	37.0	31.0	37.0
6	34.450916654861125	37.0	35.0	37.0	30.0	37.0
7	34.43446029193865	37.0	35.0	37.0	30.0	37.0
8	34.296828982124595	37.0	35.0	37.0	30.0	37.0
9	35.75595489655869	39.0	37.0	39.0	30.0	39.0
10-11	35.655385985056	39.0	36.0	39.0	30.0	39.0
12-13	35.46143486362866	39.0	35.0	39.0	29.5	39.0
14-15	36.46213530371942	40.0	36.5	41.0	28.0	41.0
16-17	36.34961999366629	40.0	36.0	41.0	28.0	41.0
18-19	36.284579668249435	40.0	36.0	41.0	28.0	41.0
20-21	35.72856746115481	39.5	35.5	41.0	26.0	41.0
22-23	35.550038438181396	39.0	35.5	41.0	25.5	41.0
24-25	35.707380428030945	39.0	36.0	41.0	26.5	41.0
26-27	35.79640784627634	39.0	35.5	41.0	27.0	41.0
28-29	35.81886888716511	40.0	35.0	41.0	27.0	41.0
30-31	35.50533783826178	39.5	35.0	40.5	26.5	41.0
32-33	34.59269930477746	38.0	35.0	40.0	24.5	41.0
34-35	34.21675539810431	38.0	34.5	40.0	23.5	41.0
36-37	33.91409381822241	37.0	34.0	40.0	20.5	41.0
38-39	33.58560656870214	37.0	33.5	40.0	17.0	41.0
40-41	33.19812082203551	37.0	33.0	40.0	12.5	41.0
42-43	32.74999526127231	37.0	33.0	40.0	6.5	41.0
44-45	32.24097368140362	36.5	33.0	40.0	2.0	41.0
46-47	31.420931670188516	36.0	31.5	40.0	2.0	41.0
48-49	30.06841375466071	36.0	27.5	40.0	2.0	41.0
50-51	28.891658600922845	35.0	23.0	40.0	2.0	41.0
52-53	28.42234835163275	35.0	20.5	40.0	2.0	41.0
54-55	28.039679612474444	35.0	17.5	40.0	2.0	41.0
56-57	27.568233418828566	35.0	11.5	40.0	2.0	41.0
58-59	27.146348868104532	35.0	7.5	39.5	2.0	41.0
60-61	26.70541222142638	34.5	6.0	39.0	2.0	41.0
62-63	26.243365913324546	34.0	2.0	39.0	2.0	41.0
64-65	25.726885410959966	34.0	2.0	38.5	2.0	40.5
66-67	25.206947899422737	33.0	2.0	38.0	2.0	40.0
68-69	24.62763462527231	33.0	2.0	37.0	2.0	40.0
70-71	24.055088898201234	32.5	2.0	36.5	2.0	39.5
72-73	23.461800488666846	32.0	2.0	36.0	2.0	39.0
74-75	22.8425447430008	31.0	2.0	35.0	2.0	39.0
76-77	22.212952990170194	30.5	2.0	35.0	2.0	37.5
78-79	21.860672522875504	30.5	2.0	35.0	2.0	37.0
80-81	21.285011437340316	30.0	2.0	35.0	2.0	37.0
82-83	20.644738872988558	29.0	2.0	35.0	2.0	36.0
84-85	20.095032740150298	29.0	2.0	35.0	2.0	36.0
86-87	19.47287015654973	27.0	2.0	34.0	2.0	35.0
88-89	18.802629003192944	25.5	2.0	34.0	2.0	35.0
90-91	18.11236505771176	23.5	2.0	34.0	2.0	35.0
92-93	17.47201282659658	19.0	2.0	34.0	2.0	35.0
94-95	16.735405544278372	6.0	2.0	34.0	2.0	35.0
96-97	16.028239002932068	2.0	2.0	33.0	2.0	35.0
98-99	15.362515442143106	2.0	2.0	33.0	2.0	35.0
100	14.547270179137115	2.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15698791096360054
1101	2	0.17103390478710878
1101	3	0.22337560037929904
1101	4	0.24864182204630225
1101	5	0.2653286501005283
1101	6	0.27132317634745107
1101	7	0.27192470172810346
1101	8	0.26292160702772094
1101	9	0.2936230714733128
1101	10-11	0.2618120098830943
1101	12-13	0.1963931295446244
1101	14-15	0.17874628253135683
1101	16-17	0.1283598255835514
1101	18-19	0.12353241535668502
1101	20-21	0.02218517260730124
1101	22-23	3.1665410785564063E-4
1101	24-25	0.05063741320857673
1101	26-27	0.1390572467490898
1101	28-29	0.20831451353043207
1101	30-31	0.24269869567793023
1101	32-33	0.27878395533791434
1101	34-35	0.27879706066740795
1101	36-37	0.25514533411992346
1101	38-39	0.16104079644252067
1101	40-41	0.16334640334620332
1101	42-43	0.12754615055456497
1101	44-45	0.1068342481150708
1101	46-47	0.1274970907498627
1101	48-49	0.16239927029878132
1101	50-51	0.09748084229759613
1101	52-53	0.0059012758291920875
1101	54-55	-0.03724223966398199
1101	56-57	-0.07819793216471282
1101	58-59	-0.14986476946438998
1101	60-61	-0.11785427197530396
1101	62-63	-0.2097906706242263
1101	64-65	-0.2040944800782043
1101	66-67	-0.27276462669996704
1101	68-69	-0.2502201751760964
1101	70-71	-0.3447159883451967
1101	72-73	-0.4266251857057455
1101	74-75	-0.43255407863498263
1101	76-77	-0.5215092536625932
1101	78-79	-0.6170444519332321
1101	80-81	-0.6089405893130717
1101	82-83	-0.5883651248037758
1101	84-85	-0.6588230357567291
1101	86-87	-0.793926103494794
1101	88-89	-0.9385577670281577
1101	90-91	-0.9937371209703407
1101	92-93	-1.0530327007131142
1101	94-95	-1.14028561216859
1101	96-97	-1.3060628754942698
1101	98-99	-1.3605596057188603
1101	100	-1.2632344711043437
1102	1	0.12544677334508947
1102	2	0.1713321122297451
1102	3	0.19950709685494417
1102	4	0.23168869095444933
1102	5	0.23393560659778245
1102	6	0.27604123385673063
1102	7	0.24562773938128402
1102	8	0.25247289190122046
1102	9	0.27799713254338343
1102	10-11	0.25118302897498523
1102	12-13	0.1962143856740184
1102	14-15	0.22960502832084018
1102	16-17	0.19779685341939057
1102	18-19	0.21759731848467823
1102	20-21	0.1432281651541203
1102	22-23	0.15172068339378342
1102	24-25	0.15750391812970577
1102	26-27	0.18825484256639413
1102	28-29	0.24074159283440366
1102	30-31	0.23137879593156185
1102	32-33	0.23539821363183222
1102	34-35	0.2646924106662212
1102	36-37	0.23135209275218216
1102	38-39	0.2002676308814415
1102	40-41	0.20694391967634118
1102	42-43	0.20779432743563575
1102	44-45	0.1649083803269349
1102	46-47	0.15673302395240185
1102	48-49	0.14174881442196963
1102	50-51	0.15353236622976496
1102	52-53	0.0941481187529618
1102	54-55	0.036380291102389606
1102	56-57	0.019680897022801958
1102	58-59	-0.026429602479378644
1102	60-61	-0.03047322629772964
1102	62-63	-0.11597396274752825
1102	64-65	-0.08387884334575091
1102	66-67	-0.11939767497486642
1102	68-69	-0.11450659633081983
1102	70-71	-0.16584165234232273
1102	72-73	-0.19895715088209087
1102	74-75	-0.2155958837929859
1102	76-77	-0.26580570796469516
1102	78-79	-0.32073482665359876
1102	80-81	-0.35155151923135364
1102	82-83	-0.35508409476569014
1102	84-85	-0.4833098403644165
1102	86-87	-0.540474987848043
1102	88-89	-0.5907759609274734
1102	90-91	-0.6587734926251407
1102	92-93	-0.7307814552003045
1102	94-95	-0.7953308243096444
1102	96-97	-0.8941459600789372
1102	98-99	-0.9174395246832088
1102	100	-0.8618831456383322
1103	1	-0.0035951660473045877
1103	2	-0.0030087377262226767
1103	3	-0.011481745634942087
1103	4	0.007655460070814968
1103	5	0.04476055007594937
1103	6	0.060016205008103896
1103	7	0.050709234953892235
1103	8	0.07085533009987444
1103	9	-0.007422563462419873
1103	10-11	0.018128926221010033
1103	12-13	-0.030113456527779192
1103	14-15	0.02641433189916853
1103	16-17	-0.021258811414092804
1103	18-19	-0.038599282962380244
1103	20-21	-0.029101397778589444
1103	22-23	0.026896798640670738
1103	24-25	0.025125786084203128
1103	26-27	-0.002390678121379608
1103	28-29	0.025363448017621693
1103	30-31	-0.010689899302221306
1103	32-33	-0.030825719378363203
1103	34-35	0.006496105698410304
1103	36-37	-0.002509635546999789
1103	38-39	-0.03976486602834228
1103	40-41	-0.009146542037704819
1103	42-43	0.010142175225659855
1103	44-45	-0.05746545668264247
1103	46-47	-0.11395396784628886
1103	48-49	-0.1507375743411501
1103	50-51	-0.10115939797937301
1103	52-53	-0.050626141837078364
1103	54-55	-0.09417685938355191
1103	56-57	-0.0919143875107693
1103	58-59	-0.15358725584959032
1103	60-61	-0.19634945039292262
1103	62-63	-0.2812306032599814
1103	64-65	-0.2980759211117565
1103	66-67	-0.26996850013897955
1103	68-69	-0.2873583924018668
1103	70-71	-0.2639012862453072
1103	72-73	-0.3452731622752516
1103	74-75	-0.30759786089145535
1103	76-77	-0.2550761068256726
1103	78-79	-0.3111646252951594
1103	80-81	-0.29606360305992396
1103	82-83	-0.2675817489699135
1103	84-85	-0.3431565830380485
1103	86-87	-0.44845049699836537
1103	88-89	-0.3694685915971263
1103	90-91	-0.3499572937620421
1103	92-93	-0.372109389983148
1103	94-95	-0.48456668310040385
1103	96-97	-0.5849869701313164
1103	98-99	-0.5663843207796742
1103	100	-0.5240779322943361
1104	1	-0.028924091427658283
1104	2	0.016167546310963843
1104	3	-0.03750637084870334
1104	4	0.04617285240136226
1104	5	-0.04960778261069976
1104	6	-0.012635037203381216
1104	7	0.007865360152820244
1104	8	-0.023957641408806296
1104	9	-0.04233771544433296
1104	10-11	-0.04685985096623568
1104	12-13	-0.012023243832118169
1104	14-15	0.05563871930861808
1104	16-17	0.04480447242803365
1104	18-19	0.0977063283572761
1104	20-21	0.04061941166494876
1104	22-23	0.006924396756382123
1104	24-25	-0.1072652663928153
1104	26-27	0.011310613117785806
1104	28-29	0.06090184927573006
1104	30-31	-8.735771378809432E-4
1104	32-33	0.06432626887563941
1104	34-35	0.09104247008534827
1104	36-37	0.028131135629472226
1104	38-39	0.07137951729269076
1104	40-41	0.046095074321719665
1104	42-43	0.01142594647850359
1104	44-45	0.04052725652292111
1104	46-47	-0.023703416484810447
1104	48-49	0.13214655489366223
1104	50-51	-0.006827752586378466
1104	52-53	0.01915596210087145
1104	54-55	0.09849262071993081
1104	56-57	0.08040338342333442
1104	58-59	0.06512684904675226
1104	60-61	0.09760958241015771
1104	62-63	0.025518269979169617
1104	64-65	0.11001248291253063
1104	66-67	0.04375690422817513
1104	68-69	0.058027433282774155
1104	70-71	0.11224267634604956
1104	72-73	0.07074552584457194
1104	74-75	0.172853203354709
1104	76-77	0.17471648079290603
1104	78-79	0.11835593333468353
1104	80-81	0.14651704268216648
1104	82-83	0.0743263766342821
1104	84-85	0.13600714590487328
1104	86-87	0.09602715358652958
1104	88-89	0.1431494460233509
1104	90-91	0.07682858475482135
1104	92-93	0.0695434458645039
1104	94-95	0.06460427381075462
1104	96-97	-0.06546553068091043
1104	98-99	-0.11350154499895204
1104	100	-0.07539258258926651
1105	1	-0.0031129037764188183
1105	2	-0.00868879218391072
1105	3	0.0013037054863431763
1105	4	0.01993769444881366
1105	5	-0.026501835806875818
1105	6	0.028656133096305325
1105	7	-0.025463079210901185
1105	8	0.007086923374451715
1105	9	0.04276770191381729
1105	10-11	0.016492602732775197
1105	12-13	0.07141780737614312
1105	14-15	0.060868917666262234
1105	16-17	0.0328289001555504
1105	18-19	0.02742279213676113
1105	20-21	0.017373630526506645
1105	22-23	-0.0011608089688763812
1105	24-25	0.007105327861893329
1105	26-27	0.043341466006125984
1105	28-29	-0.010078002945810738
1105	30-31	0.05708849295500329
1105	32-33	0.05679801624612679
1105	34-35	-0.0373043128187831
1105	36-37	-0.014668968511863056
1105	38-39	1.6735998904238159E-4
1105	40-41	0.0910568735047903
1105	42-43	0.09321926628796717
1105	44-45	0.1555419606505808
1105	46-47	0.15438770441642546
1105	48-49	0.20085492150723638
1105	50-51	0.30425830093914286
1105	52-53	0.2426210302655143
1105	54-55	0.22307867981558616
1105	56-57	0.10337971393681045
1105	58-59	0.25872875807886686
1105	60-61	0.22142560872728723
1105	62-63	0.21879072138225908
1105	64-65	0.20362112497439
1105	66-67	0.25012240981156353
1105	68-69	0.29816366475309053
1105	70-71	0.31731276012103393
1105	72-73	0.2291795396289409
1105	74-75	0.21229008194261212
1105	76-77	0.28923492055001887
1105	78-79	0.29063148273030137
1105	80-81	0.27471772913308357
1105	82-83	0.31487307039601475
1105	84-85	0.41083771037545347
1105	86-87	0.39194361505988695
1105	88-89	0.4476897211644726
1105	90-91	0.42684056467305354
1105	92-93	0.3901569215068186
1105	94-95	0.3717887669605844
1105	96-97	0.34092819066508184
1105	98-99	0.35809780455562823
1105	100	0.38174819676692273
1106	1	-0.21981657083913575
1106	2	-0.18272319368458767
1106	3	-0.17634499654758073
1106	4	-0.09782352439458464
1106	5	-0.1488179647966703
1106	6	-0.1110377735351662
1106	7	-0.15758042307184894
1106	8	-0.1464773803857966
1106	9	-0.21959399460070017
1106	10-11	-0.1459290778128164
1106	12-13	-0.09502496258888726
1106	14-15	-0.07897207529681083
1106	16-17	-0.09959227312179308
1106	18-19	-0.05370623508026284
1106	20-21	-0.044119483112638136
1106	22-23	-0.05545947736133172
1106	24-25	-0.07457372335802859
1106	26-27	-0.1033266704782676
1106	28-29	-0.17091003570233454
1106	30-31	-0.19530680055133587
1106	32-33	-0.16798171664066075
1106	34-35	-0.09937874266960733
1106	36-37	-0.16148201751417446
1106	38-39	-0.11588856047109886
1106	40-41	-0.10921306079793425
1106	42-43	-0.12251023781394821
1106	44-45	-0.11518187511902056
1106	46-47	-0.14247317864255749
1106	48-49	-0.1764585959004279
1106	50-51	-0.05454942300955068
1106	52-53	-0.03137299022474238
1106	54-55	-0.054981522037465425
1106	56-57	-0.002078156258249919
1106	58-59	0.012502917046095519
1106	60-61	0.038498263290790646
1106	62-63	-0.0501321368752663
1106	64-65	0.00867064964263875
1106	66-67	-0.019452279594705857
1106	68-69	0.019789207285967336
1106	70-71	0.07100536913418054
1106	72-73	0.061122120458065865
1106	74-75	0.1419086251664119
1106	76-77	0.1810931935747675
1106	78-79	0.20594081790470398
1106	80-81	0.23270377861606661
1106	82-83	0.290765234880908
1106	84-85	0.26465856413834743
1106	86-87	0.23802757946107178
1106	88-89	0.2508564389873911
1106	90-91	0.2711270198975697
1106	92-93	0.17399400385666652
1106	94-95	0.2463725245339532
1106	96-97	0.14344192788177423
1106	98-99	0.1641312932855108
1106	100	0.18039237772373617
1107	1	-0.242188310923396
1107	2	-0.2412571602864162
1107	3	-0.2425157189521343
1107	4	-0.19916143093394112
1107	5	-0.22089179904403977
1107	6	-0.31957105223578
1107	7	-0.277590188626597
1107	8	-0.20688702921915336
1107	9	-0.2539474112629492
1107	10-11	-0.2090593052208476
1107	12-13	-0.15218574379577632
1107	14-15	-0.18255100598518226
1107	16-17	-0.16193152527396393
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2208	1	-0.9089139038840095
2208	2	-0.658534072752996
2208	3	-0.6270471863617004
2208	4	-0.5162081636823075
2208	5	-0.7493037348845775
2208	6	-0.6488804003151358
2208	7	-0.6654116681223456
2208	8	-0.5934319492250495
2208	9	-0.7042772825002857
2208	10-11	-0.6643541159894326
2208	12-13	-0.6530937456828099
2208	14-15	-0.6929871586999994
2208	16-17	-0.6769186232838962
2208	18-19	-0.6747916281457123
2208	20-21	-0.6594947348319522
2208	22-23	-0.6168265764415679
2208	24-25	-0.788827048525448
2208	26-27	-0.8039981564650702
2208	28-29	-0.9259920582648888
2208	30-31	-1.028590242160604
2208	32-33	-1.0269129289015453
2208	34-35	-0.9561710818400755
2208	36-37	-0.9755915743010348
2208	38-39	-0.9586517614722894
2208	40-41	-0.9510166465003138
2208	42-43	-0.9882181640575283
2208	44-45	-0.9932361403051182
2208	46-47	-0.9678298992984935
2208	48-49	-0.8828333757196027
2208	50-51	-0.8495899691488553
2208	52-53	-0.7539889430103557
2208	54-55	-0.6758956828935467
2208	56-57	-0.6992135442736114
2208	58-59	-1.186452600764536
2208	60-61	-0.8395696761683311
2208	62-63	-0.7454362591564525
2208	64-65	-0.6719627397229786
2208	66-67	-0.5783751019196934
2208	68-69	-0.5596259526527874
2208	70-71	-0.41394370849038253
2208	72-73	-0.34738910972385284
2208	74-75	-0.3441512173088732
2208	76-77	-0.31057413585269344
2208	78-79	-0.2663087954409953
2208	80-81	-0.17147814574702025
2208	82-83	-0.10095085376283564
2208	84-85	-0.07970094060761213
2208	86-87	-0.07855033593331129
2208	88-89	-0.01559248080347686
2208	90-91	-0.7977900543068799
2208	92-93	-0.40867381056797925
2208	94-95	-0.12119671588547831
2208	96-97	0.26438277004533717
2208	98-99	0.38403281595444305
2208	100	0.33263041760253564
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	518554.0
3	178142.0
4	165275.0
5	149306.0
6	130095.0
7	152822.0
8	187155.0
9	201727.0
10	206678.0
11	216437.0
12	248829.0
13	317379.0
14	434308.0
15	548912.0
16	651778.0
17	787645.0
18	1208112.0
19	968860.0
20	635326.0
21	540035.0
22	534621.0
23	560789.0
24	597271.0
25	641420.0
26	688122.0
27	735925.0
28	779066.0
29	831133.0
30	895509.0
31	969020.0
32	1059098.0
33	1208724.0
34	1415541.0
35	1675798.0
36	2210223.0
37	3107471.0
38	3203394.0
39	720521.0
40	1369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.844581288634213	39.212544498885954	23.620463002433972	19.322411210045857
2	20.056585802644854	22.98074518960203	38.26278823211467	18.69988077563844
3	20.982706215835574	37.555827920007246	21.47222862928569	19.98923723487149
4	21.38797563414719	20.584480757817246	37.54214419121344	20.485399416822126
5	31.04426242599615	20.83203873149028	20.944398051790596	27.17930079072297
6	30.996245233865178	20.927400146543572	27.231386884944797	20.844967734646456
7	22.222116878479163	30.22227015913108	20.404221560575422	27.15139140181433
8	29.128395712750276	21.019737661393368	29.22189035031883	20.629976275537523
9	21.770883836071366	27.983425918107567	20.473643170232698	29.77204707558837
10-11	32.919183676570526	20.754797820655575	20.727381348689246	25.598637154084653
12-13	28.788291788194208	20.911171355638622	21.057816997205954	29.242719858961213
14-15	28.01261184013441	21.372185872264275	22.293279885412492	28.32192240218882
16-17	27.283667857072192	21.290489745184864	22.957936688853664	28.46790570888928
18-19	26.55125972468218	21.269538243348933	24.76736251059095	27.41183952137794
20-21	21.854463050161442	24.585663131157435	30.647108270452293	22.912765548228826
22-23	21.67052422366321	24.612242026219935	31.375407074936803	22.341826675180048
24-25	17.358063235064957	20.438152577310053	36.55517053750606	25.64861365011893
26-27	17.33995300719673	20.42721255717726	44.99251013491335	17.240324300712654
28-29	17.31083463814432	20.488448700776203	45.02956977957842	17.171146881501052
30-31	17.294789482914087	20.459813398165807	45.017930720638574	17.22746639828153
32-33	17.336566272225134	20.50972443061985	44.91932961685073	17.234379680304286
34-35	17.385526241215707	20.57883931120483	44.86172361554182	17.173910832037645
36-37	17.416564689717585	20.63297711587719	44.67300162337394	17.27745657103128
38-39	17.514532862531805	20.758779684135963	44.37336090971951	17.353326543612724
40-41	17.627435978609295	20.998268681826136	43.92622889808889	17.448066441475678
42-43	17.8282896358374	21.271224465975358	43.205902373133995	17.694583525053247
44-45	18.024893233394305	21.711155732206986	42.393022108154035	17.870928926244673
46-47	18.360317824410792	22.312669135483723	41.2262708226335	18.10074221747198
48-49	18.953522298542296	23.537392958451726	38.817180383409465	18.691904359596514
50-51	19.627851281226175	25.357393765875084	35.62486504616251	19.389889906736222
52-53	20.06561506959916	26.67337437099376	33.41295573585683	19.84805482355025
54-55	20.30784724772103	27.507076428065663	31.914347044178342	20.270729280034967
56-57	20.499884846748735	28.278451048802932	30.7787472928251	20.442916811623235
58-59	20.630604672859214	28.913540357690675	29.936430286676487	20.519424682773625
60-61	20.638856439951166	29.44838662663346	29.249656125292887	20.663100808122486
62-63	20.739995626445616	29.9543379191475	28.714778880299736	20.59088757410715
64-65	20.826988833586878	30.457289277666494	28.149884677407915	20.56583721133871
66-67	20.938192384133064	30.821347867229616	27.643861218939037	20.59659852969828
68-69	21.052244037608933	31.233788572844844	27.072275641774564	20.641691747771656
70-71	21.254774709669576	31.627229312564015	26.554313530102842	20.563682447663567
72-73	21.405015287905922	31.971823373200138	25.901742537729795	20.721418801164152
74-75	21.630461253339877	32.249385174502635	25.440352253729326	20.679801318428158
76-77	21.56355035100371	32.63174153982785	24.91858290809674	20.886125201071703
78-79	21.747544304949713	32.67185464265048	24.58900494858878	20.991596103811023
80-81	21.929322878579644	32.72825033916476	24.254014212058774	21.08841257019682
82-83	22.05583832500713	32.72569474404084	23.88924520733344	21.3292217236186
84-85	22.216864308719394	32.5726044803592	23.614886696973812	21.595644513947594
86-87	22.365590840250967	32.416694752938355	23.337881745940418	21.87983266087026
88-89	22.57242105534359	32.193138889283425	23.06052844802044	22.173911607352544
90-91	22.6979867931123	31.94495034757375	22.749025431551654	22.60803742776229
92-93	22.899211454361676	31.69548830295932	22.66290456429341	22.742395678385595
94-95	23.011770509178707	31.546448605828754	22.383230100394986	23.058550784597557
96-97	23.072737159802518	31.44047472167121	22.15103390500325	23.33575421352302
98-99	23.511074694185368	31.105027022251498	22.001445114760486	23.382453168802645
100	24.614277481744203	30.667439399444262	21.022025131374374	23.696257987437157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5184.0
1	3786.0
2	2388.0
3	2333.5
4	2294.5
5	2241.0
6	2243.5
7	2527.0
8	3056.5
9	3911.5
10	5797.5
11	9021.5
12	11178.0
13	19477.5
14	44479.0
15	73821.5
16	354194.5
17	440100.0
18	235091.0
19	208092.0
20	207175.5
21	214993.5
22	228759.5
23	249206.5
24	270117.5
25	290058.5
26	313260.5
27	341212.0
28	372608.0
29	405355.0
30	436071.5
31	466007.5
32	498335.0
33	532745.5
34	568216.5
35	612313.5
36	667552.0
37	735004.5
38	804532.5
39	855797.0
40	898875.0
41	945719.0
42	1005933.5
43	1063724.5
44	1100508.0
45	1113358.0
46	1105168.0
47	1082419.0
48	1046600.0
49	1015814.0
50	971532.5
51	908911.5
52	842114.0
53	767395.5
54	697536.0
55	634870.5
56	571964.5
57	514872.0
58	464975.5
59	418025.5
60	374336.5
61	331348.0
62	290726.0
63	254892.0
64	222503.5
65	192129.0
66	164835.0
67	140535.5
68	118881.0
69	99356.0
70	82232.5
71	67663.5
72	55082.0
73	45057.0
74	36977.5
75	30071.5
76	24188.0
77	19259.5
78	15275.0
79	12198.5
80	9494.5
81	7230.0
82	5573.5
83	4119.5
84	2960.5
85	2186.5
86	1543.5
87	1038.5
88	752.5
89	533.0
90	381.5
91	257.0
92	175.0
93	124.5
94	80.0
95	51.0
96	28.5
97	19.5
98	13.0
99	9.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17032011013661735
2	0.07197582489360979
3	0.06819144724045889
4	0.08731477271113673
5	0.09430563439675667
6	0.03807493397978165
7	0.03672101178275559
8	0.029707034352308388
9	0.03881463781425442
10-11	0.03448373790840155
12-13	0.02883524054739405
14-15	0.02949899264886292
16-17	0.0295650376340837
18-19	0.03132348536558706
20-21	0.030669640011901308
22-23	0.028003073733612174
24-25	0.02944780778531681
26-27	0.021390319588381234
28-29	0.020782705724350026
30-31	0.01981184444160451
32-33	0.01952619988052462
34-35	0.016702776762336127
36-37	0.01961536061057268
38-39	0.023815821670614506
40-41	0.02279707777358392
42-43	0.030639919768551953
44-45	0.03125413813110524
46-47	0.03078356761140716
48-49	0.02734592613066538
50-51	0.026910029228208208
52-53	0.027233649655790046
54-55	0.02853803811390052
56-57	0.024824658819861973
58-59	0.020667127000213656
60-61	0.010544081890498075
62-63	0.008818656651605108
64-65	0.006262715723560788
66-67	0.007524174941277753
68-69	0.012503966826924822
70-71	0.018756775802702495
72-73	0.016595453661352354
74-75	0.016169463506678302
76-77	0.021461317947493574
78-79	0.019082047354914852
80-81	0.01187488834269686
82-83	0.014485316383548325
84-85	0.017383040110110198
86-87	0.02188070360364555
88-89	0.025427319310001625
90-91	0.03053589891682922
92-93	0.0843691663702898
94-95	0.1319017422336876
96-97	0.08644297890622239
98-99	0.06976166676408302
100	0.07490822223741257
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.028239E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.374606808168345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70006129738007	34.569588213607766
2	10.563482321962436	9.163737844803158
3	3.729240787526132	4.852630585557901
4	1.7354106792192996	3.01091023447334
5	0.9819598321438009	2.129606081032618
6	0.6356432899229799	1.6542466662395887
7	0.44962538649027833	1.3651627044990586
8	0.3377634183759207	1.172028437299074
9	0.265100791548806	1.034877833816729
>10	1.3444853459921167	10.160889217436988
>50	0.11950038779588364	3.743066548634132
>100	0.13222742461157377	10.712242602163675
>500	0.003420898283412312	0.9415035084148723
>1k	0.0016061509231597422	1.3529772257071173
>5k	1.6202567098723934E-4	0.5542795124356283
>10k+	3.099621531755061E-4	13.582252783878149
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	1569408	5.1825764082689645	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	1269322	4.191617636520763	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA	105887	0.3496652675036548	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	104140	0.34389623804461933	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	91028	0.30059714573387375	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73055	0.2412458197652167	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA	72944	0.24087927009724133	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67041	0.22138609270932708	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	60078	0.19839253110471136	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	58176	0.19211165301021485	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	58143	0.19200267878460053	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG	53428	0.17643257351880084	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49431	0.16323348322242728	No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46883	0.15481935210529948	No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46073	0.15214453020385774	No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT	43056	0.14218164418330256	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41134	0.13583472110358527	No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37279	0.12310455020227927	No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36700	0.12119254788013759	No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35918	0.11861018895800497	No Hit
TCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34848	0.11507678224869305	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC	33336	0.11008378136600182	No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33286	0.10991866890294986	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTAAA	32960	0.10884213564385109	No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31030	0.1024687945700455	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG	30956	0.1022244281247286	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3022492610391717E-6	0.0	0.0	0.0	0.0
2	3.3022492610391717E-6	0.0	0.0	0.0	0.0
3	6.604498522078343E-6	6.604498522078343E-6	0.0	3.3022492610391717E-6	0.0
4	6.604498522078343E-6	1.3208997044156687E-5	0.0	3.3022492610391717E-6	3.3022492610391717E-6
5	6.604498522078343E-6	1.3208997044156687E-5	0.0	6.604498522078343E-6	3.3022492610391717E-6
6	6.604498522078343E-6	1.651124630519586E-5	0.0	6.604498522078343E-6	3.3022492610391717E-6
7	6.604498522078343E-6	1.651124630519586E-5	0.0	6.604498522078343E-6	6.604498522078343E-6
8	9.906747783117515E-6	1.651124630519586E-5	0.0	6.604498522078343E-6	6.604498522078343E-6
9	9.906747783117515E-6	2.3115744827274203E-5	0.0	6.604498522078343E-6	6.604498522078343E-6
10-11	9.906747783117515E-6	2.3115744827274203E-5	0.0	6.604498522078343E-6	6.604498522078343E-6
12-13	1.4860121674676272E-5	3.302249261039172E-5	8.25562315259793E-6	6.604498522078343E-6	6.604498522078343E-6
14-15	3.962699113247006E-5	4.292924039350923E-5	9.906747783117515E-6	6.604498522078343E-6	8.25562315259793E-6
16-17	7.430060837338137E-5	4.953373891558757E-5	9.906747783117515E-6	6.604498522078343E-6	9.906747783117515E-6
18-19	2.1464620196754615E-4	5.613823743766592E-5	9.906747783117515E-6	8.25562315259793E-6	9.906747783117515E-6
20-21	4.5240814876236653E-4	7.430060837338137E-5	9.906747783117515E-6	9.906747783117515E-6	9.906747783117515E-6
22-23	0.001127718122644877	7.595173300390095E-5	9.906747783117515E-6	9.906747783117515E-6	1.1557872413637101E-5
24-25	0.0020952771561293547	7.760285763442053E-5	9.906747783117515E-6	9.906747783117515E-6	1.3208997044156687E-5
26-27	0.0033072026349307304	8.585848078701846E-5	9.906747783117515E-6	9.906747783117515E-6	1.3208997044156687E-5
28-29	0.00721541463537059	9.081185467857723E-5	9.906747783117515E-6	9.906747783117515E-6	1.3208997044156687E-5
30-31	0.010332737937791568	9.576522857013598E-5	9.906747783117515E-6	9.906747783117515E-6	1.3208997044156687E-5
32-33	0.010742216846160425	9.906747783117515E-5	9.906747783117515E-6	1.1557872413637101E-5	1.651124630519586E-5
34-35	0.011460456060436445	1.0071860246169473E-4	9.906747783117515E-6	1.651124630519586E-5	1.651124630519586E-5
36-37	0.012467642085053392	1.0402085172273391E-4	9.906747783117515E-6	1.651124630519586E-5	1.651124630519586E-5
38-39	0.013740659175183993	1.0567197635325349E-4	9.906747783117515E-6	1.981349556623503E-5	1.981349556623503E-5
40-41	0.01526794945841461	1.1392759950585142E-4	9.906747783117515E-6	1.981349556623503E-5	2.4766869457793786E-5
42-43	0.017247647890407593	1.1888097339741017E-4	9.906747783117515E-6	2.1464620196754616E-5	2.6417994088313373E-5
44-45	0.01982175118938763	1.2383434728896893E-4	9.906747783117515E-6	2.6417994088313373E-5	2.6417994088313373E-5
46-47	0.023422854008550844	1.3374109507208645E-4	9.906747783117515E-6	2.6417994088313373E-5	2.6417994088313373E-5
48-49	0.02818799969223037	1.4199671822468437E-4	9.906747783117515E-6	3.137136797987213E-5	2.6417994088313373E-5
50-51	0.03471159310741325	1.5685683989936065E-4	9.906747783117515E-6	4.127811576298964E-5	2.6417994088313373E-5
52-53	0.042392624888590365	1.6676358768247818E-4	9.906747783117515E-6	4.6231489654548406E-5	2.6417994088313373E-5
54-55	0.05302916975839754	1.7006583694351734E-4	1.1557872413637101E-5	4.953373891558757E-5	2.6417994088313373E-5
56-57	0.0670207998774205	1.750192108350761E-4	1.3208997044156687E-5	4.953373891558757E-5	2.9720243349352544E-5
58-59	0.08477864527865865	1.865770832487132E-4	1.3208997044156687E-5	5.1184863546107156E-5	2.9720243349352544E-5
60-61	0.10703910754732371	2.0639057881494824E-4	1.3208997044156687E-5	5.448711280714634E-5	2.9720243349352544E-5
62-63	0.13534103483905993	2.0804170344546782E-4	1.3208997044156687E-5	5.613823743766592E-5	3.302249261039172E-5
64-65	0.17024085615435242	2.1134395270650699E-4	1.3208997044156687E-5	5.7789362068185504E-5	3.302249261039172E-5
66-67	0.21258724955328823	2.1629732659806574E-4	1.3208997044156687E-5	6.274273595974425E-5	3.302249261039172E-5
68-69	0.2634732595412713	2.2125070048962451E-4	1.981349556623503E-5	6.604498522078343E-5	3.6324741871430884E-5
70-71	0.3260888589044656	2.2785519901170285E-4	1.981349556623503E-5	6.604498522078343E-5	4.127811576298964E-5
72-73	0.39921881991480856	2.2950632364222243E-4	1.981349556623503E-5	6.93472344818226E-5	4.292924039350923E-5
74-75	0.4842236692678484	2.3611082216430077E-4	1.981349556623503E-5	7.264948374286177E-5	4.292924039350923E-5
76-77	0.5833505875857223	2.4766869457793785E-4	1.981349556623503E-5	7.595173300390095E-5	4.6231489654548406E-5
78-79	0.6963436505506996	2.542731931000162E-4	2.1464620196754616E-5	7.595173300390095E-5	5.1184863546107156E-5
80-81	0.8254252719154598	2.707844394052121E-4	2.3115744827274203E-5	7.925398226494012E-5	5.2835988176626747E-5
82-83	0.9694759891805105	2.773889379272904E-4	2.3115744827274203E-5	8.585848078701846E-5	5.613823743766592E-5
84-85	1.1286493569364902	2.8399343644936874E-4	2.3115744827274203E-5	9.081185467857723E-5	5.613823743766592E-5
86-87	1.3036537076498915	2.905979349714471E-4	2.3115744827274203E-5	9.246297930909681E-5	5.613823743766592E-5
88	1.4459063501923064	2.939001842324863E-4	2.6417994088313373E-5	9.576522857013598E-5	5.944048669870509E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	230315	0.0	89.615036	1
TATCTCG	230860	0.0	88.72703	2
ATATGGA	288505	0.0	87.80535	1
TATGGAT	287690	0.0	87.73435	2
ATGGATC	294920	0.0	85.37206	3
GGATCCG	301290	0.0	82.96955	5
TGGATCC	302740	0.0	82.844604	4
ATCTCGA	251585	0.0	80.88983	3
TCTCGAG	260300	0.0	77.88647	4
GATCCGG	322545	0.0	77.45591	6
ATCCGGC	331530	0.0	74.39742	7
CTCGAGG	272220	0.0	74.36617	5
TCGAGGG	283575	0.0	71.29925	6
TCCGGCG	345400	0.0	71.17237	8
CCGGCGC	366480	0.0	66.9926	9
CGAGGGC	303640	0.0	66.28246	7
GAGGGCG	324495	0.0	62.03319	8
AGGGCGC	325550	0.0	61.586746	9
CCGTCGA	320015	0.0	34.525467	16-17
GTCGACT	319275	0.0	34.41485	18-19
>>END_MODULE