FastQCFastQC Report
Wed 25 May 2016
SRR490973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31911442
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT13707744.295556433958703No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT11479393.597264579895825No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1275040.3995557455535854No Hit
ATATGGATCCGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1160580.3636877330707901No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTA954840.2992155603623302No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA916740.28727626912002285No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG779680.24432615737013702No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC712670.22332741967598957No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAA660430.20695711588338753No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA576870.18077215062860524No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTG548330.17182865004972198No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT505380.158369527770008No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT465550.1458881112298216No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG423540.1327235541408627No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTC339610.10642264301312364No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC331140.10376842262408575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC2254700.089.209921
TATCTCG2255600.088.761342
TATGGAT2494600.087.063012
ATGGATC2505500.086.472153
ATATGGA2540300.085.85381
GGATCCG2523050.085.252325
TGGATCC2550850.084.454544
ATCTCGA2371900.083.820283
TCTCGAG2421800.081.804924
GATCCGG2660900.080.833686
CTCGAGG2498600.079.247365
ATCCGGC2745450.078.0280767
TCGAGGG2574600.076.827256
TCCGGCG2867550.074.903378
CCGGCGC2994550.071.863229
CGAGGGC2755000.071.6473857
AGGGCGC2870350.068.718339
GAGGGCG2904600.068.034238
CCGTCGA2615750.037.3774116-17
GTCGACT2618500.037.2466618-19
Produced by FastQC (version 0.11.5)