FastQCFastQC Report
Wed 25 May 2016
SRR490972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58235449
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA901200.15475110357610533No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT643310.11046707993957426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA390750.044.5776331
TCTCGAG483550.035.988722
TATCTCG225550.029.3864862
CTCGAGG601700.028.798923
GATCCGG697700.024.9389341
ATATCTC279550.024.449411
TCGAGGG761850.022.731244
ATCCGGC808750.021.4594572
TCCGGCG965100.018.047493
TATGGAT448750.016.3310092
GCGCCGT1064400.016.2671979
GGATCCG467150.015.7984572
CGGCGCG1101050.015.71116355
CGAGGGC1131150.015.48846155
AGGGCGC1121300.015.3567767
ATATGGA494850.015.2187861
ATGGATC508950.014.5481013
GGGCGCG1198900.014.3901748
CGTCGAC1018600.014.10843612-13
CCGGCGC1244350.014.0305034
Produced by FastQC (version 0.11.5)