FastQCFastQC Report
Wed 25 May 2016
SRR490971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38896352
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA465940.11979015409979835No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420670.10815153050856799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA290000.046.294431
TCTCGAG357900.037.419572
TATCTCG156750.031.0684992
CTCGAGG449600.029.6746393
ATATCTC187950.026.6550851
GATCCGG480100.025.6121851
TCGAGGG562000.023.6780954
ATCCGGC553000.022.254232
TCCGGCG658350.018.7586943
GCGCCGT737500.016.5342359
CGAGGGC815300.016.436955
CGGCGCG750200.016.3088785
TATGGAT283950.016.2900092
AGGGCGC832350.015.8862587
GGATCCG322200.015.0564372
GGGCGCG886950.014.8869128
ATATGGA321100.014.6791251
CCGGCGC843600.014.6426664
ATGGATC347050.014.313461
GAGGGCG938450.014.2451976
Produced by FastQC (version 0.11.5)