FastQCFastQC Report
Wed 25 May 2016
SRR490970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52735750
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA306900.040.9739651
TCTCGAG381250.032.9322472
TATCTCG169050.029.4357782
CTCGAGG472250.026.4376343
GATCCGG606150.024.3143311
ATATCTC236250.021.8163571
ATCCGGC685500.021.3660432
TCGAGGG617900.020.213174
TCCGGCG822300.017.8747273
GGATCCG403200.016.2573012
GCGCCGT910500.015.9812419
TATGGAT388850.015.76969052
CGGCGCG945400.015.4227765
ATATGGA435600.014.5311721
CGTCGAC869350.014.23384212-13
AGGGCGC896750.014.1372612
CGAGGGC897150.014.0367365
CCGGCGC1073050.013.7063564
TCGACTT927700.013.42111814-15
GCCGTCG952350.013.16030810-11
Produced by FastQC (version 0.11.5)