FastQCFastQC Report
Wed 25 May 2016
SRR490969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53928812
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT657520.12192369451787664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA390400.050.339531
TCTCGAG492400.039.8227042
CTCGAGG631050.030.9098323
TATCTCG193850.028.8214912
GATCCGG716850.028.2745461
ATCCGGC828500.024.4277112
TCGAGGG798750.024.4260584
ATATCTC241450.023.742321
TCCGGCG1005650.020.195083
CGAGGGC1141250.017.2231245
GCGCCGT1196250.016.8036949
CGGCGCG1209050.016.6848185
AGGGCGC1170700.016.6189467
GGATCCG462250.016.1961881
TATGGAT412450.015.790372
CCGGCGC1307250.015.5393714
GGGCGCG1265950.015.4060318
ATGGATC499950.015.3699381
GAGGGCG1339300.014.6572036
ATATGGA461450.014.440821
Produced by FastQC (version 0.11.5)