FastQCFastQC Report
Wed 25 May 2016
SRR490968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62850846
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA733850.1167605603908657No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT657140.10455547408224226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA386500.047.7642561
TCTCGAG492500.037.3459742
CTCGAGG607650.030.0052153
GATCCGG698100.025.5147041
TCGAGGG799500.022.8321254
TATCTCG237750.022.8291992
ATCCGGC821250.021.7095662
ATATCTC289050.019.4938091
TCCGGCG1028500.017.3299393
GCGCCGT1092700.016.2131779
CGGCGCG1107300.016.0061725
CGAGGGC1162450.015.8815745
AGGGCGC1148600.015.7975667
GGATCCG472500.015.6542462
GGGCGCG1226650.014.8463598
CGTCGAC1043550.013.96253412-13
CCGGCGC1283850.013.9548194
ATGGATC535650.013.4169891
GAGGGCG1371400.013.4123636
TCGACTT1111550.013.22306214-15
Produced by FastQC (version 0.11.5)