FastQCFastQC Report
Wed 25 May 2016
SRR490967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48487342
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA599640.12366938983786738No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT548800.1131841790791502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA320250.044.634021
TCTCGAG409450.034.807352
TATCTCG192050.029.6787722
CTCGAGG498100.028.5366443
ATATCTC222150.026.4871671
GATCCGG580950.025.71381
TCGAGGG642700.022.1125954
ATCCGGC691050.021.534662
TCCGGCG846250.017.7238583
GGATCCG391700.017.5265352
GCGCCGT911000.016.2813599
TATGGAT389050.015.785912
CGGCGCG940300.015.7593585
CGAGGGC961000.014.9650795
AGGGCGC956900.014.8251517
ATGGATC436750.014.5710011
TGGATCC491950.014.3223851
CCGGCGC1049450.014.2496654
CGTCGAC873300.014.17923612-13
GGGCGCG1019100.013.9482738
Produced by FastQC (version 0.11.5)