FastQCFastQC Report
Wed 25 May 2016
SRR490966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53970537
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA869650.16113421291324193No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT561400.10401971727648365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA340150.046.46731
TCTCGAG419300.037.649742
CTCGAGG517400.030.2545783
TATCTCG192300.025.9229032
GATCCGG596750.025.2804721
TCGAGGG659050.023.7236064
ATCCGGC702450.021.4769382
ATATCTC247600.020.8797681
TCCGGCG865150.017.4691683
AGGGCGC963650.016.1865187
GCGCCGT927650.016.0955589
CGAGGGC990550.015.9409535
CGGCGCG953450.015.6841475
GGATCCG402250.015.6736093
GGGCGCG1024950.015.2321478
ATGGATC453850.014.490171
CGTCGAC892300.013.93877412-13
GAGGGCG1140650.013.8802626
CCGGCGC1087250.013.8791184
TCGACTT943550.013.284492514-15
Produced by FastQC (version 0.11.5)