##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR490966_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53970537
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.958142717757283	31.0	31.0	34.0	28.0	34.0
2	31.276300456302668	33.0	31.0	34.0	28.0	34.0
3	31.379966721472496	34.0	31.0	34.0	28.0	34.0
4	34.79199286084554	37.0	35.0	37.0	32.0	37.0
5	34.41389578910434	37.0	35.0	37.0	30.0	37.0
6	34.40482211618536	37.0	35.0	37.0	31.0	37.0
7	34.34141340857883	37.0	35.0	37.0	30.0	37.0
8	34.27701319703378	37.0	35.0	37.0	30.0	37.0
9	35.75823027293577	39.0	37.0	39.0	30.0	39.0
10-11	35.75635027496577	39.0	37.0	39.0	30.0	39.0
12-13	35.71437402966734	39.0	37.0	39.0	30.0	39.0
14-15	36.883239590519544	40.0	37.0	41.0	30.0	41.0
16-17	36.7824992921601	40.0	37.0	41.0	30.0	41.0
18-19	36.714075033198206	40.0	37.0	41.0	30.0	41.0
20-21	36.61715733901258	40.0	37.0	41.0	30.0	41.0
22-23	36.4536353232876	40.0	37.0	41.0	29.0	41.0
24-25	36.30843716637469	40.0	36.0	41.0	28.5	41.0
26-27	36.0185934132914	40.0	36.0	41.0	27.5	41.0
28-29	35.74895167339172	39.0	35.0	41.0	27.0	41.0
30-31	35.46247842596045	39.0	35.0	41.0	26.5	41.0
32-33	35.18865284775654	39.0	35.0	41.0	25.0	41.0
34-35	35.13726195646339	39.0	35.0	41.0	25.0	41.0
36-37	35.04324117619953	39.0	35.0	41.0	24.5	41.0
38-39	34.92163237138071	39.0	35.0	41.0	24.0	41.0
40-41	34.71221668592995	39.0	35.0	41.0	22.5	41.0
42-43	34.42084498807192	39.0	34.0	41.0	19.5	41.0
44-45	34.06583819056682	39.0	33.5	41.0	16.0	41.0
46-47	33.67740203326122	38.5	33.0	41.0	10.5	41.0
48-49	33.29563391225846	38.0	33.0	40.5	7.0	41.0
50-51	33.000388739878574	38.0	33.0	40.0	2.0	41.0
52-53	32.628372902793245	38.0	32.0	40.0	2.0	41.0
54-55	32.268491918099684	38.0	31.5	40.0	2.0	41.0
56-57	31.873339040521312	37.0	31.0	40.0	2.0	41.0
58-59	31.395781442752735	37.0	30.5	40.0	2.0	41.0
60-61	30.947999841098486	36.0	29.5	40.0	2.0	41.0
62-63	30.44896322784411	35.5	29.0	39.0	2.0	41.0
64-65	29.974655366130598	35.0	28.0	39.0	2.0	41.0
66-67	29.378640720213696	35.0	27.5	39.0	2.0	40.5
68-69	28.86055047960705	35.0	26.0	38.0	2.0	40.0
70-71	28.25472092301027	34.0	26.0	37.0	2.0	40.0
72-73	27.651155027418014	34.0	24.5	37.0	2.0	39.0
74-75	27.026567282441526	34.0	22.5	36.0	2.0	39.0
76-77	26.23792688962869	32.5	21.5	35.0	2.0	38.0
78-79	26.264500888697846	33.0	20.5	35.0	2.0	37.0
80-81	25.82895723272125	33.0	18.5	35.0	2.0	37.0
82-83	25.264344840593303	33.0	14.0	35.0	2.0	36.5
84-85	24.674826850435082	32.0	6.0	35.0	2.0	36.0
86-87	24.123168368697165	32.0	2.0	35.0	2.0	36.0
88-89	23.522298647871523	31.0	2.0	35.0	2.0	35.0
90-91	22.989417531643237	31.0	2.0	35.0	2.0	35.0
92-93	22.372994269076848	31.0	2.0	34.5	2.0	35.0
94-95	21.71401286038714	30.0	2.0	34.0	2.0	35.0
96-97	21.003387561254023	29.5	2.0	34.0	2.0	35.0
98-99	20.293178090853534	29.0	2.0	34.0	2.0	35.0
100	19.316751341569937	27.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17218041540163398
1101	2	0.16153630028815869
1101	3	0.17447359305780807
1101	4	0.17198866439161264
1101	5	0.28021625600572264
1101	6	0.2170908114604586
1101	7	0.212448885268941
1101	8	0.2618350709149482
1101	9	0.31430223440878535
1101	10-11	0.24825657828262848
1101	12-13	0.19955294696919168
1101	14-15	0.1833918301447568
1101	16-17	0.20334428532679993
1101	18-19	0.14385387720706433
1101	20-21	0.11766486794492437
1101	22-23	0.14851038550075657
1101	24-25	0.11535437936444026
1101	26-27	0.1345366418566698
1101	28-29	0.061967432960173596
1101	30-31	-0.021984042513658153
1101	32-33	-0.08226600449066979
1101	34-35	-0.08239962808335122
1101	36-37	-0.7719417374766095
1101	38-39	-0.47904094475199344
1101	40-41	-0.21684698336466823
1101	42-43	-0.26324537454842556
1101	44-45	-0.2071952115997462
1101	46-47	-0.3006971685687603
1101	48-49	-0.3464196107471693
1101	50-51	-0.3913285425171722
1101	52-53	-0.3309282333809165
1101	54-55	-0.43141797408976146
1101	56-57	-0.4993047165567539
1101	58-59	-0.5467552777823776
1101	60-61	-0.598210054327545
1101	62-63	-0.73210502701329
1101	64-65	-0.7743862162469277
1101	66-67	-0.8003090764653393
1101	68-69	-0.8297832589611147
1101	70-71	-0.8400080059869595
1101	72-73	-0.8271798036575575
1101	74-75	-0.8098783244100467
1101	76-77	-0.7014811176984956
1101	78-79	-0.754053048249876
1101	80-81	-0.7342531025032244
1101	82-83	-0.7465932067825385
1101	84-85	-0.7186618282833201
1101	86-87	-0.7461530995606616
1101	88-89	-0.768067035630736
1101	90-91	-0.8059671916763946
1101	92-93	-0.8941617057199416
1101	94-95	-0.903441384059402
1101	96-97	-0.9077318377414123
1101	98-99	-0.9333081886205754
1101	100	-0.8748989805747271
1102	1	0.06903747973282037
1102	2	0.05335295935699236
1102	3	0.05653717183466256
1102	4	0.10895975066338082
1102	5	0.20140443988487533
1102	6	0.1028984595984781
1102	7	0.14915885876482804
1102	8	0.1343154917868148
1102	9	0.18300768613673313
1102	10-11	0.16587135618253512
1102	12-13	0.1419259857039279
1102	14-15	0.1348883579886575
1102	16-17	0.18414383511750287
1102	18-19	0.1805085780172817
1102	20-21	0.1653089228937077
1102	22-23	0.23997328366911574
1102	24-25	0.17783711169115435
1102	26-27	0.25019279050050613
1102	28-29	0.19214184802682155
1102	30-31	0.13729972861911222
1102	32-33	0.059127143560004924
1102	34-35	0.14600713767585205
1102	36-37	0.14254617205893538
1102	38-39	0.11191263621412162
1102	40-41	0.09565698521154076
1102	42-43	0.02280319148767518
1102	44-45	0.08198292448258115
1102	46-47	0.02599881708472651
1102	48-49	0.04361354470906065
1102	50-51	-0.02104776124465957
1102	52-53	-0.05889461739302959
1102	54-55	-0.145854709356513
1102	56-57	-0.15257151262528978
1102	58-59	-0.15371176627202132
1102	60-61	-0.20482871087730814
1102	62-63	-0.2523078079696788
1102	64-65	-0.3156789717651769
1102	66-67	-0.35049940991795836
1102	68-69	-0.4039947794137184
1102	70-71	-0.478789062911936
1102	72-73	-0.5523445323008751
1102	74-75	-0.5679434972923154
1102	76-77	-0.46399844191353523
1102	78-79	-0.4954750492593867
1102	80-81	-0.5227664301115595
1102	82-83	-0.522898713026688
1102	84-85	-0.5533056348478276
1102	86-87	-0.627701334693505
1102	88-89	-0.6276872525264423
1102	90-91	-0.6923407200204039
1102	92-93	-0.817813064404735
1102	94-95	-0.8151721693430609
1102	96-97	-0.8264349823645425
1102	98-99	-0.9090507606695226
1102	100	-0.865272275939418
1103	1	0.11887943620806496
1103	2	0.1400764295775936
1103	3	0.09634647004566688
1103	4	0.1176820695930374
1103	5	0.23970155946135918
1103	6	0.20478775342407118
1103	7	0.20873516680281767
1103	8	0.2249799048586567
1103	9	0.23666352729464535
1103	10-11	0.23742833785883732
1103	12-13	0.21782306962716547
1103	14-15	0.2152815882153405
1103	16-17	0.2560612393111441
1103	18-19	0.22551297237184542
1103	20-21	0.2124593840133997
1103	22-23	0.286789613314852
1103	24-25	0.21095654434243016
1103	26-27	0.30139001178859104
1103	28-29	0.307836746777717
1103	30-31	0.031993411918207926
1103	32-33	0.13304314283930552
1103	34-35	0.2213628298274557
1103	36-37	0.21370143824477594
1103	38-39	0.269756900878356
1103	40-41	0.3005705704860091
1103	42-43	0.31675088655720884
1103	44-45	0.3434452935723371
1103	46-47	0.31082464285043443
1103	48-49	0.2600918130185548
1103	50-51	0.21151618996702837
1103	52-53	0.26611708831184444
1103	54-55	0.25156101556126487
1103	56-57	0.24087851586132913
1103	58-59	0.2047748772813911
1103	60-61	0.24360651387863896
1103	62-63	0.17619481469894538
1103	64-65	0.1833021836674611
1103	66-67	0.10983231242563107
1103	68-69	0.06506198535369734
1103	70-71	0.16290596067633345
1103	72-73	0.199915444492067
1103	74-75	0.19351738351615566
1103	76-77	0.20012322730952192
1103	78-79	-0.02148155450723621
1103	80-81	0.08705236048267295
1103	82-83	0.0548945417368607
1103	84-85	-0.048543298357625275
1103	86-87	0.05449477269914027
1103	88-89	0.03311005102204234
1103	90-91	0.05903587831031487
1103	92-93	-0.0017651544418164633
1103	94-95	-0.0186258757119937
1103	96-97	0.02543911748083616
1103	98-99	-0.05030783246383308
1103	100	0.002414080500674487
1104	1	0.0520078576416978
1104	2	0.06938769804926181
1104	3	0.03456065504267869
1104	4	0.06685279392976895
1104	5	0.12941281887102463
1104	6	0.11013733698273143
1104	7	0.10089031967970641
1104	8	0.047355435059095896
1104	9	0.03579525721058019
1104	10-11	0.02722134877074467
1104	12-13	-0.054270569803946955
1104	14-15	-0.02644737262641428
1104	16-17	-0.11879704504855226
1104	18-19	-0.017386350939560202
1104	20-21	-0.010890368184135468
1104	22-23	0.08816537766141153
1104	24-25	0.11531280066723326
1104	26-27	0.14807552647944533
1104	28-29	0.18550968958711422
1104	30-31	0.19370256689609988
1104	32-33	0.12671702718444067
1104	34-35	0.1964035862572331
1104	36-37	0.2136705431244721
1104	38-39	0.19421281715430183
1104	40-41	0.10070957968520844
1104	42-43	0.02802980702205815
1104	44-45	0.08626681767384525
1104	46-47	0.11789716786894644
1104	48-49	0.21152960603441784
1104	50-51	0.0980814979532596
1104	52-53	0.1448066371420893
1104	54-55	0.1022580386590306
1104	56-57	0.19824758154825517
1104	58-59	0.20463261015663647
1104	60-61	-0.0077443770597867
1104	62-63	0.06841559952042786
1104	64-65	0.16460001753549847
1104	66-67	0.18790521457044917
1104	68-69	0.18519320377820847
1104	70-71	0.1834816583568255
1104	72-73	0.27426802929071314
1104	74-75	0.3359199194619187
1104	76-77	0.3178350219060455
1104	78-79	0.24287071220838286
1104	80-81	0.2048996796123994
1104	82-83	0.2113846950972622
1104	84-85	0.29436497409561113
1104	86-87	0.38087426725002516
1104	88-89	0.39600588466895204
1104	90-91	0.2980816095111507
1104	92-93	0.25041645851571914
1104	94-95	0.21061537948092024
1104	96-97	0.24636995887204804
1104	98-99	0.18773704858737972
1104	100	0.16507257386244234
1105	1	0.03129129169261091
1105	2	0.02027189195083423
1105	3	0.015548630131753782
1105	4	-0.015084378095714612
1105	5	-0.14232814007730354
1105	6	-0.012165497933672498
1105	7	0.0547880516798358
1105	8	0.026061021545814356
1105	9	0.01900333505216878
1105	10-11	0.016293909467002266
1105	12-13	-0.05073611276898049
1105	14-15	-0.01336795039051708
1105	16-17	-0.05933976437367505
1105	18-19	-0.025701806900428892
1105	20-21	-0.07554009271174777
1105	22-23	-0.31639480446316526
1105	24-25	-0.2481248377656513
1105	26-27	-0.07792103263771821
1105	28-29	-0.0878915962887703
1105	30-31	-0.052670768538312984
1105	32-33	0.04452816661672898
1105	34-35	0.09687030794997042
1105	36-37	0.11926158955694888
1105	38-39	0.049593932164093246
1105	40-41	-0.1373450970869854
1105	42-43	-0.0859847153506621
1105	44-45	-0.1022782764747987
1105	46-47	0.01775793024592076
1105	48-49	-0.028007338939666226
1105	50-51	-0.16272061249151193
1105	52-53	-0.2840419565843959
1105	54-55	-0.07733467095543034
1105	56-57	-0.07279202168914267
1105	58-59	-0.13318852571137896
1105	60-61	-0.11447760688992048
1105	62-63	0.010630996496338696
1105	64-65	0.14449688377423087
1105	66-67	0.2399773203989426
1105	68-69	0.261972488372745
1105	70-71	0.29058087703835866
1105	72-73	0.20472460673206072
1105	74-75	0.2466844274533635
1105	76-77	0.23008847890959672
1105	78-79	0.2648442492163703
1105	80-81	0.30938425424848504
1105	82-83	0.36185867441529496
1105	84-85	0.3756186349981654
1105	86-87	0.41165487316031246
1105	88-89	0.41775207775739176
1105	90-91	0.37610950604728544
1105	92-93	0.40144283010473814
1105	94-95	0.41465038349880246
1105	96-97	0.3927291742120609
1105	98-99	0.3180060951067212
1105	100	0.23312876386216175
1106	1	-0.4003336388906362
1106	2	-0.27584002635375526
1106	3	-0.2106613926554992
1106	4	-0.12864983254259954
1106	5	-0.09667654442992557
1106	6	-0.11294059476983165
1106	7	-0.1521545356068188
1106	8	-0.28453368169430604
1106	9	-0.46031395954905463
1106	10-11	-0.2560055989691463
1106	12-13	-0.1999710130969632
1106	14-15	-0.22351598015907825
1106	16-17	-0.2060812787878774
1106	18-19	-0.23288547565591955
1106	20-21	-0.26884966165856383
1106	22-23	-0.19594514803743834
1106	24-25	-0.22301155885646295
1106	26-27	-0.14709582761880569
1106	28-29	-0.16023135185641024
1106	30-31	-0.24549943168507582
1106	32-33	-0.22162231703148194
1106	34-35	-0.13725486420140953
1106	36-37	-0.09289906630525735
1106	38-39	-0.1960838967044225
1106	40-41	-0.07007011366994931
1106	42-43	-0.08293539932021332
1106	44-45	-0.030149987327192207
1106	46-47	-0.0028026582903635244
1106	48-49	-0.035319123799745
1106	50-51	-0.02325147566056529
1106	52-53	0.011327413821945242
1106	54-55	0.020990722681951013
1106	56-57	-1.9053396940549305E-4
1106	58-59	0.03762876305876617
1106	60-61	0.09804342348216721
1106	62-63	0.0986405138217421
1106	64-65	0.10124671946271846
1106	66-67	0.07611496388693695
1106	68-69	0.0959612914186998
1106	70-71	0.08524024325987511
1106	72-73	0.126872807428132
1106	74-75	0.16694660278895057
1106	76-77	0.21113202801803155
1106	78-79	0.18719105685455872
1106	80-81	0.1972710261827828
1106	82-83	0.16999231927426095
1106	84-85	0.22430646876674132
1106	86-87	0.21489100487551482
1106	88-89	0.26885977431614805
1106	90-91	0.31221908350352834
1106	92-93	0.3057862973512968
1106	94-95	0.37866498181491437
1106	96-97	0.4261465077321134
1106	98-99	0.39056691100129726
1106	100	0.3381675069963208
1107	1	-0.2008850546839014
1107	2	-0.22001174774422694
1107	3	-0.2004728073535631
1107	4	-0.1890417364166126
1107	5	-0.21123112612582418
1107	6	-0.266472943526999
1107	7	-0.21627538640051114
1107	8	-0.23925053852240552
1107	9	-0.1746866914927594
1107	10-11	-0.31624594812316786
1107	12-13	-0.33195946033624324
1107	14-15	-0.39609640435588744
1107	16-17	-0.46616486166071525
1107	18-19	-0.40932873955363647
1107	20-21	-0.48447506414720465
1107	22-23	-0.3334523002060621
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2208	1	-0.6154736192470338
2208	2	-0.6763655495539069
2208	3	-0.6941677279270522
2208	4	-0.5840059524279653
2208	5	-0.9738133779962652
2208	6	-0.798239157057516
2208	7	-0.6881636599021022
2208	8	-0.662797525459105
2208	9	-0.907999026333556
2208	10-11	-1.1149407663940352
2208	12-13	-1.0611520837533206
2208	14-15	-1.15173147670005
2208	16-17	-1.2055742166318453
2208	18-19	-1.2009977773956706
2208	20-21	-1.1369470241604347
2208	22-23	-1.0375366504489065
2208	24-25	-1.1074022029258188
2208	26-27	-0.9570529173980802
2208	28-29	-0.7932541492328085
2208	30-31	-1.0276870829935518
2208	32-33	-0.7907531570184503
2208	34-35	-0.9752762806172299
2208	36-37	-0.7779429993579683
2208	38-39	-0.9006079223883958
2208	40-41	-0.6907196664002129
2208	42-43	-0.6185291108743982
2208	44-45	-0.6458552587063551
2208	46-47	-0.547770687899316
2208	48-49	-0.46582746493299254
2208	50-51	-0.4763117033208175
2208	52-53	-0.4143215990641309
2208	54-55	-0.41883809853208476
2208	56-57	-0.3848684445891166
2208	58-59	-0.16408107057075583
2208	60-61	-0.10420672489204463
2208	62-63	0.011545025537380127
2208	64-65	-0.11010159885179505
2208	66-67	-0.024843627309060423
2208	68-69	0.1627060547614967
2208	70-71	0.30533833150798984
2208	72-73	0.26334437605152416
2208	74-75	0.2832954861138184
2208	76-77	0.32065080106573873
2208	78-79	0.4424874549765967
2208	80-81	0.5723887009982889
2208	82-83	0.6786611879543898
2208	84-85	0.8311257993726748
2208	86-87	0.7454402622720977
2208	88-89	0.9098815524057713
2208	90-91	1.071312787732733
2208	92-93	1.2495518995261925
2208	94-95	1.291971822362072
2208	96-97	1.3652981093369831
2208	98-99	1.5017011517609085
2208	100	1.429879219510127
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1185934.0
3	364384.0
4	210170.0
5	173809.0
6	169848.0
7	184340.0
8	200845.0
9	223246.0
10	251970.0
11	279024.0
12	306589.0
13	374402.0
14	466927.0
15	597769.0
16	701581.0
17	701045.0
18	645234.0
19	587372.0
20	586597.0
21	613437.0
22	654251.0
23	708468.0
24	772927.0
25	854217.0
26	948116.0
27	1063663.0
28	1206717.0
29	1369642.0
30	1561714.0
31	1797830.0
32	2101696.0
33	2527035.0
34	3143292.0
35	4071526.0
36	5556151.0
37	7722815.0
38	7404296.0
39	1679073.0
40	2585.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.036150599380107	28.082145237847495	27.77630409763201	22.105400065140394
2	23.754247281718392	27.632126880214386	27.079454694305877	21.534171143761345
3	23.67791432390914	26.423451482452915	26.864302967825974	23.034331225811968
4	24.647366575690054	25.984550645567328	26.587421325451317	22.780661453291305
5	24.775151051068995	26.340766023364914	26.078827742718207	22.80525518284788
6	24.72878148404914	26.135446131835273	25.69256162588365	23.443210758231942
7	24.581799806472343	26.508202857437364	25.854786218605835	23.055211117484458
8	24.1176653915442	26.31630760049552	26.129347201703002	23.43667980625728
9	23.959976617970412	26.082349020890483	26.289630327799777	23.66804403333933
10-11	23.864674945269897	26.259425168856886	26.3127039750114	23.56319591086182
12-13	23.555367362700423	26.31521113594716	26.5150381860462	23.614383315306213
14-15	23.252911881859966	26.564389244646662	26.938447958609252	23.244250914884123
16-17	23.109513091228706	26.448058470890757	27.180373434875733	23.262055003004807
18-19	22.715289487351583	26.457218313800617	27.873795356359242	22.953696842488558
20-21	22.40877651207289	26.222009412677284	28.618972635214572	22.75024144003525
22-23	22.430280150775072	26.18439672410695	28.975681556463417	22.40964156865456
24-25	22.32304096771317	26.051760416340297	29.395329670709003	22.22986894523753
26-27	22.340421055222976	26.115853029606345	29.44861243131451	22.09511348385617
28-29	22.40715153909131	26.16010116842148	29.411786354713087	22.020960937774127
30-31	22.377346804332092	26.121727370301606	29.38042002975021	22.12050579561609
32-33	22.39897165643627	26.190655731814267	29.264602449309734	22.14577016243973
34-35	22.432774338715614	26.23259017369401	29.319056976219287	22.015578511371096
36-37	22.426441990918885	26.245077298592616	29.201578439638904	22.12690227084959
38-39	22.432216531221595	26.326309428257854	29.096052988488907	22.14542105203165
40-41	22.486659215213677	26.489378834623384	28.86528262338393	22.158679326779012
42-43	22.614566975700708	26.602230883181978	28.447237234453958	22.335964906663357
44-45	22.68395967655002	26.84932552243689	28.02352448090103	22.443190320112063
46-47	22.902683804822445	27.036418123761386	27.581662607351376	22.47923546406479
48-49	22.97359164868792	27.0842213955932	27.240525296556296	22.701661659162585
50-51	22.967271391011135	27.204268806426988	27.050407542302207	22.778052260259667
52-53	23.056263765291714	27.296367295346823	26.910177018320507	22.737191921040957
54-55	23.09417624093312	27.283124246020694	26.813092431675056	22.80960708137113
56-57	23.06817237769482	27.383400616721865	26.756731653625494	22.79169535195782
58-59	23.161198098855635	27.456339284684166	26.697194659718704	22.68526795674149
60-61	23.122083304422	27.45063717951354	26.65132797914452	22.77595153691994
62-63	23.151605890388407	27.529204442363376	26.61042306433057	22.70876660291765
64-65	23.17474780841806	27.62171595322152	26.5676484669382	22.635887771422222
66-67	23.192985882489406	27.65097679668943	26.457117985136673	22.698919335684494
68-69	23.191711483265102	27.77737037497851	26.358966877602697	22.671951264153694
70-71	23.254936740221478	27.866069390642622	26.282804261349984	22.59618960778592
72-73	23.236386265312703	27.910484971275896	26.15089712269813	22.702231640713265
74-75	23.281646734332632	28.016417069407414	26.10156943679258	22.60036675946737
76-77	23.103361780708237	28.26169021737494	25.91150702375391	22.723440978162913
78-79	23.175902268618884	28.28668753286081	25.809921817596653	22.727488380923656
80-81	23.168000829860073	28.39503155377818	25.700722316810555	22.7362452995512
82-83	23.205546617343373	28.52090265939518	25.58856183232347	22.684988890937973
84-85	23.2014220113727	28.563403218258205	25.45282421314087	22.782350557228217
86-87	23.20816145412506	28.724918433153135	25.301031425336852	22.765888687384955
88-89	23.35318475423326	28.802685039982258	25.109148629572143	22.73498157621234
90-91	23.339983368950872	28.829753572795262	24.981178943459046	22.849084114794817
92-93	23.34910464098197	28.9841851095407	24.837047366702215	22.829662882775114
94-95	23.504370197058407	29.00911268453988	24.725175013279475	22.761342105122235
96-97	23.450518191125607	29.12548815057363	24.460674339367497	22.963319318933266
98-99	23.470026161856612	29.20249977485015	24.35335758651221	22.974116476781028
100	24.023693189823682	29.07888566586178	23.67295583126985	23.224465313044693
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14818.0
1	10217.0
2	5039.0
3	4301.5
4	4296.0
5	4570.0
6	4954.0
7	5545.5
8	6386.5
9	7489.0
10	8853.5
11	10961.0
12	13939.5
13	19659.5
14	31876.5
15	42966.5
16	58261.5
17	62973.0
18	58989.5
19	67289.5
20	82048.5
21	98303.5
22	114539.0
23	136949.5
24	164314.0
25	196555.0
26	234358.0
27	277799.5
28	332232.5
29	391598.0
30	446325.0
31	515358.0
32	614124.5
33	730705.0
34	846145.0
35	979871.0
36	1147036.0
37	1331425.5
38	1500982.0
39	1621955.0
40	1725117.0
41	1881508.5
42	2119980.0
43	2332948.5
44	2488253.5
45	2589513.0
46	2580545.5
47	2467663.0
48	2324336.5
49	2212991.5
50	2105287.0
51	1969389.0
52	1804823.0
53	1632886.0
54	1476347.5
55	1336349.0
56	1188259.5
57	1043009.5
58	926216.5
59	824939.5
60	722457.5
61	628162.5
62	542647.5
63	466543.5
64	402609.0
65	345516.5
66	294849.5
67	249038.0
68	206570.5
69	169612.5
70	138090.0
71	113290.5
72	94116.0
73	79400.5
74	66222.0
75	52147.5
76	40418.0
77	32798.0
78	27540.0
79	22214.0
80	16109.0
81	10968.0
82	7480.0
83	4901.0
84	3026.0
85	1825.0
86	1152.0
87	731.0
88	454.0
89	300.0
90	184.0
91	94.5
92	46.0
93	23.5
94	14.0
95	7.0
96	4.5
97	2.5
98	2.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12122910691068352
2	0.008430525714428226
3	0.0012247423070850677
4	4.7433287536123646E-4
5	0.005302893317515074
6	6.114447221453438E-5
7	0.0018343341664360318
8	0.0015174946285970807
9	0.0016397835730261492
10-11	0.002983109098951526
12-13	0.008560226110034813
14-15	0.007923767740165342
16-17	0.01905669384019655
18-19	0.021939748348251565
20-21	0.020324051991552353
22-23	0.02472089540261569
24-25	0.027723459560908204
26-27	0.04971045591041646
28-29	0.0643582627313862
30-31	0.05011715929378283
32-33	0.06820573232391591
34-35	0.07631293348072486
36-37	0.09723638658625909
38-39	0.03137267283443928
40-41	0.025425909695877217
42-43	0.03327463649287018
44-45	0.041182654899283286
46-47	0.05025149184637536
48-49	0.045703640117570075
50-51	0.04365437386698598
52-53	0.04604271400894158
54-55	0.042650122232432114
56-57	0.03728515801130532
58-59	0.028051416275513434
60-61	0.009728456101891297
62-63	0.012653200022819859
64-65	0.007274339330735212
66-67	0.005200985863824182
68-69	0.007443876276420967
70-71	0.0013683391736495044
72-73	0.001949211659687581
74-75	0.004068886696458106
76-77	0.00185193636298264
78-79	0.002637550187799688
80-81	0.008916902197952931
82-83	0.0011747150116368122
84-85	9.347692797646242E-4
86-87	0.0035556437024148934
88-89	1.723162398773242E-4
90-91	7.411451177519319E-4
92-93	8.940062982882678E-4
94-95	0.0035537908396205136
96-97	0.002604198657500851
98-99	0.012000992319198158
100	0.003611229586246288
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5.3970537E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.36423610551157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.82100756130272	31.411855231732243
2	11.963880543890177	9.658257980234387
3	4.112171917387021	4.979540345395918
4	1.879009040248982	3.0337905818000244
5	1.0232727867904101	2.065181218317646
6	0.6426409592299108	1.5563826845657134
7	0.4338788690829698	1.2259232379000138
8	0.30543511326446854	0.9862924021376543
9	0.22086074158225097	0.8023387607697919
>10	1.2694211597922316	9.806569820664386
>50	0.14478208122221503	4.074549804782269
>100	0.13593682101241097	12.607590588790671
>500	0.03420156612885898	9.532302267379645
>1k	0.013404083234142833	7.256900747876485
>5k	1.8429682253190913E-5	0.06625545753869805
>10k+	7.832614890283339E-5	0.9362688701142559
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	86965	0.16113421291324193	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56140	0.10401971727648365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.85286279437983E-6	1.85286279437983E-6	1.85286279437983E-6	3.70572558875966E-6
2	0.0	1.85286279437983E-6	1.85286279437983E-6	3.70572558875966E-6	5.55858838313949E-6
3	0.0	1.85286279437983E-6	1.85286279437983E-6	3.70572558875966E-6	9.264313971899149E-6
4	0.0	1.85286279437983E-6	1.85286279437983E-6	5.55858838313949E-6	1.111717676627898E-5
5	1.85286279437983E-6	3.70572558875966E-6	1.85286279437983E-6	9.264313971899149E-6	1.2970039560658809E-5
6	3.70572558875966E-6	1.111717676627898E-5	1.85286279437983E-6	1.111717676627898E-5	1.2970039560658809E-5
7	5.55858838313949E-6	1.8528627943798297E-5	1.85286279437983E-6	1.482290235503864E-5	1.2970039560658809E-5
8	9.264313971899149E-6	1.8528627943798297E-5	1.85286279437983E-6	1.8528627943798297E-5	1.482290235503864E-5
9	9.264313971899149E-6	2.223435353255796E-5	1.85286279437983E-6	2.0381490738178128E-5	1.8528627943798297E-5
10-11	2.0381490738178128E-5	2.5940079121317617E-5	1.85286279437983E-6	2.5940079121317617E-5	2.1307922135368042E-5
12-13	6.114447221453438E-5	2.7792941915697448E-5	1.85286279437983E-6	3.1498667504457106E-5	2.7792941915697448E-5
14-15	1.1209819905997972E-4	3.1498667504457106E-5	1.85286279437983E-6	3.4277961696026854E-5	2.964580471007728E-5
16-17	2.4087216326937788E-4	3.4277961696026854E-5	2.779294191569745E-6	3.891011868197643E-5	3.4277961696026854E-5
18-19	4.5302495322586836E-4	4.3542275667926E-5	3.70572558875966E-6	4.817443265387558E-5	3.891011868197643E-5
20-21	7.272486467940831E-4	4.6321569859495745E-5	3.70572558875966E-6	5.8365178022964643E-5	4.539513846230583E-5
22-23	0.001130246304571696	4.910086405106549E-5	3.70572558875966E-6	6.577662920048395E-5	4.817443265387558E-5
24-25	0.0016240342392739209	5.1880158242635234E-5	3.70572558875966E-6	7.226164898081336E-5	5.1880158242635234E-5
26-27	0.002207686019503567	5.1880158242635234E-5	3.70572558875966E-6	8.523168854147217E-5	5.651231522858481E-5
28-29	0.002873790194083116	5.373302103701507E-5	3.70572558875966E-6	9.634886530775116E-5	6.114447221453438E-5
30-31	0.0033675781287853404	5.743874662577473E-5	3.70572558875966E-6	1.0190745369089064E-4	6.855592339205371E-5
32-33	0.003793736571492702	5.929160942015456E-5	3.70572558875966E-6	1.0376031648527047E-4	7.782023736395286E-5
34-35	0.004292156663180876	6.021804081734447E-5	3.70572558875966E-6	1.0839247347122005E-4	8.615811993866208E-5
36-37	0.004948070092391336	6.670306059767387E-5	3.70572558875966E-6	1.1580392464873937E-4	9.26431397189915E-5
38-39	0.005657716542638811	6.948235478924362E-5	3.70572558875966E-6	1.2599467001782842E-4	1.0098102229370073E-4
40-41	0.0064507418186333775	7.504094317238312E-5	3.70572558875966E-6	1.2877396420939816E-4	1.0653961067684022E-4
42-43	0.00751243219981302	8.615811993866208E-5	4.632156985949575E-6	1.2970039560658808E-4	1.0746604207403014E-4
44-45	0.008801098273304192	9.264313971899149E-5	5.55858838313949E-6	1.3803827818129733E-4	1.1395106185435953E-4
46-47	0.010502026318544876	9.820172810213099E-5	5.55858838313949E-6	1.4544972935881664E-4	1.1858321884030911E-4
48-49	0.012610584178549121	1.0098102229370073E-4	5.55858838313949E-6	1.5286118053633595E-4	1.2136251303187886E-4
50-51	0.01513418330449445	1.0376031648527047E-4	5.55858838313949E-6	1.6027263171385528E-4	1.3062682700377802E-4
52-53	0.018495276413499462	1.0746604207403014E-4	5.55858838313949E-6	1.7046337708294433E-4	1.3711184678410741E-4
54-55	0.022692010642769773	1.0839247347122005E-4	5.55858838313949E-6	1.7694839686327376E-4	1.3989114097567716E-4
56-57	0.027993051097490472	1.1487749325154945E-4	5.55858838313949E-6	1.8343341664360315E-4	1.4637616075600655E-4
58-59	0.0346503871176972	1.1487749325154945E-4	5.55858838313949E-6	1.8899200502674266E-4	1.5100831774195612E-4
60-61	0.042916008043425616	1.1858321884030911E-4	5.55858838313949E-6	2.0381490738178128E-4	1.5193474913914606E-4
62-63	0.053459723774844044	1.2228894442906877E-4	5.55858838313949E-6	2.1215278995649053E-4	1.5564047472790572E-4
64-65	0.06672158922561768	1.250682386206385E-4	5.55858838313949E-6	2.1771137833963001E-4	1.5656690612509563E-4
66-67	0.08346498386703101	1.2692110141501834E-4	5.55858838313949E-6	2.2141710392838967E-4	1.611990631110452E-4
68-69	0.1044023334435231	1.2784753281220825E-4	5.55858838313949E-6	2.2697569231152915E-4	1.7138980848013428E-4
70-71	0.13001538228163267	1.2970039560658808E-4	5.55858838313949E-6	2.3346071209185857E-4	1.7880125965765358E-4
72-73	0.1616391180247104	1.3062682700377802E-4	5.55858838313949E-6	2.3716643768061823E-4	1.8343341664360315E-4
74-75	0.20071970008376977	1.3155325840096793E-4	5.55858838313949E-6	2.4365145746094762E-4	1.862127108351729E-4
76-77	0.24843647562743354	1.3155325840096793E-4	5.55858838313949E-6	2.510629086384669E-4	1.9177129921831238E-4
78-79	0.30595952751035255	1.3247968979815785E-4	5.55858838313949E-6	2.5847435981598627E-4	1.9640345620426197E-4
80-81	0.3739179026512187	1.3711184678410741E-4	6.485019780329404E-6	2.72370830773835E-4	2.0288847598459137E-4
82-83	0.4547814671549405	1.4174400377005698E-4	9.264313971899149E-6	2.8070871334854425E-4	2.0752063297054094E-4
84-85	0.5495794863037957	1.426704351672469E-4	9.264313971899149E-6	2.8348800754011397E-4	2.112263585593006E-4
86-87	0.6599434057882358	1.4544972935881664E-4	9.264313971899149E-6	2.853408703344938E-4	2.1400565275087036E-4
88	0.7528329762588799	1.500818863447662E-4	9.264313971899149E-6	2.908994587176333E-4	2.1493208414806027E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	34015	0.0	46.4673	1
TCTCGAG	41930	0.0	37.64974	2
CTCGAGG	51740	0.0	30.254578	3
TATCTCG	19230	0.0	25.922903	2
GATCCGG	59675	0.0	25.280472	1
TCGAGGG	65905	0.0	23.723606	4
ATCCGGC	70245	0.0	21.476938	2
ATATCTC	24760	0.0	20.879768	1
TCCGGCG	86515	0.0	17.469168	3
AGGGCGC	96365	0.0	16.186518	7
GCGCCGT	92765	0.0	16.095558	9
CGAGGGC	99055	0.0	15.940953	5
CGGCGCG	95345	0.0	15.684147	5
GGATCCG	40225	0.0	15.673609	3
GGGCGCG	102495	0.0	15.232147	8
ATGGATC	45385	0.0	14.49017	1
CGTCGAC	89230	0.0	13.938774	12-13
GAGGGCG	114065	0.0	13.880262	6
CCGGCGC	108725	0.0	13.879118	4
TCGACTT	94355	0.0	13.2844925	14-15
>>END_MODULE