FastQCFastQC Report
Wed 25 May 2016
SRR490965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58021187
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590280.10173525060767888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA380450.046.230381
TCTCGAG473450.037.1622772
CTCGAGG581150.029.965813
TATCTCG222900.027.6744672
ATATCTC259050.024.3508851
GATCCGG717200.023.9792461
TCGAGGG743000.023.4069144
ATCCGGC831100.020.7122272
TCCGGCG1026000.016.8542773
GGATCCG497400.016.6710742
TATGGAT464550.016.3531782
GCGCCGT1072450.015.9063339
CGAGGGC1117550.015.7472955
AGGGCGC1106300.015.7380997
CGGCGCG1087800.015.6899465
ATGGATC507250.014.8735683
GGGCGCG1177150.014.7827488
TGGATCC589150.014.5077021
CCGGCGC1216500.014.1455994
CGTCGAC1030800.013.94587412-13
Produced by FastQC (version 0.11.5)