FastQCFastQC Report
Wed 25 May 2016
SRR490964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31651487
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1289470.4073963412840604No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT861490.27217994528977424No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT783580.24756498802094196No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT596290.18839241265347187No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT452700.143026455597489No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364490.11515730682732221No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320370.10121799332840191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG241450.055.50242
ATATCTC282350.047.75371
ATCTCGA361450.037.036863
TATGGAT303100.036.9555662
ATATGGA312000.036.1688961
TCTCGAG409750.032.4648484
ATGGATC364300.030.6311973
GGATCCG381100.028.8741825
CTCGAGG462100.028.5022665
TGGATCC431500.025.8174534
GATCCGG557250.024.8043461
TCGAGGG548050.023.9083636
ATCCGGC616800.022.214472
TCCGGCG724750.019.0224443
CGAGGGC726600.018.111387
GCGCCGT821650.016.7945799
CGGCGCG828500.016.6008015
GAGGGCG806300.016.2862878
AGGGCGC817850.015.976119
CCGGCGC885200.015.6117584
Produced by FastQC (version 0.11.5)