##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR490964_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31651487 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99059744017714 34.0 31.0 34.0 30.0 34.0 2 32.214162070805706 34.0 31.0 34.0 30.0 34.0 3 32.274681944642914 34.0 31.0 34.0 30.0 34.0 4 35.6160629672786 37.0 35.0 37.0 35.0 37.0 5 35.416227521948656 37.0 35.0 37.0 33.0 37.0 6 35.391710632742154 37.0 35.0 37.0 33.0 37.0 7 35.35294825168877 37.0 35.0 37.0 33.0 37.0 8 35.29545218523224 37.0 35.0 37.0 33.0 37.0 9 37.02166479571718 39.0 38.0 39.0 34.0 39.0 10-11 36.97669556567753 39.0 38.0 39.0 34.0 39.0 12-13 36.912972603783196 39.0 38.0 39.0 34.0 39.0 14-15 38.29305950143828 41.0 39.0 41.0 34.0 41.0 16-17 38.25756898878085 41.0 39.0 41.0 34.0 41.0 18-19 38.159024961449674 41.0 38.5 41.0 33.5 41.0 20-21 38.10833950708224 41.0 39.0 41.0 33.5 41.0 22-23 38.044562250740384 41.0 38.0 41.0 33.0 41.0 24-25 37.92571608089061 40.5 38.0 41.0 33.0 41.0 26-27 37.7056962284268 40.0 38.0 41.0 33.0 41.0 28-29 37.49470443521342 40.0 38.0 41.0 32.5 41.0 30-31 37.29207115924759 40.0 37.0 41.0 32.0 41.0 32-33 37.080240969405324 40.0 37.0 41.0 31.5 41.0 34-35 36.86791258811948 40.0 36.0 41.0 31.0 41.0 36-37 36.66325683213556 40.0 36.0 41.0 30.0 41.0 38-39 36.38575454606603 40.0 35.5 41.0 30.0 41.0 40-41 36.03955872594548 40.0 35.0 41.0 29.0 41.0 42-43 35.90193414925498 40.0 35.0 41.0 28.0 41.0 44-45 35.860792527693874 40.0 35.0 41.0 27.0 41.0 46-47 35.692596038220884 40.0 35.0 41.0 26.0 41.0 48-49 35.44047802556638 40.0 35.0 41.0 24.0 41.0 50-51 35.19645588847058 40.0 35.0 41.0 23.5 41.0 52-53 34.92414830304813 39.5 35.0 41.0 22.5 41.0 54-55 34.66315272960161 39.0 35.0 41.0 20.5 41.0 56-57 34.376281136491315 39.0 35.0 41.0 18.5 41.0 58-59 34.071407498169044 39.0 34.0 41.0 15.5 41.0 60-61 33.745917119154626 38.0 34.0 41.0 11.5 41.0 62-63 33.368562178453104 37.5 33.5 40.5 7.5 41.0 64-65 33.00108595213868 37.0 33.0 40.0 6.0 41.0 66-67 32.58920651974424 36.5 33.0 40.0 2.0 41.0 68-69 32.16991566620551 36.0 32.5 39.0 2.0 41.0 70-71 31.73402921006523 35.0 32.0 39.0 2.0 41.0 72-73 31.271013601983377 35.0 31.5 38.5 2.0 40.5 74-75 30.79205856900183 35.0 31.0 37.0 2.0 40.0 76-77 30.065713121156044 35.0 30.0 37.0 2.0 39.0 78-79 29.99196146771872 35.0 31.0 36.0 2.0 39.0 80-81 29.640121773741626 35.0 30.0 36.0 2.0 38.0 82-83 29.240338787242443 35.0 30.0 35.5 2.0 37.0 84-85 28.846841145251723 35.0 29.5 35.0 2.0 37.0 86-87 28.43651571567554 34.5 29.0 35.0 2.0 36.5 88-89 28.038766993790844 34.0 29.0 35.0 2.0 36.0 90-91 27.626097282570008 34.0 27.5 35.0 2.0 36.0 92-93 27.248497993159056 34.0 27.0 35.0 2.0 35.5 94-95 26.827605492910966 34.0 25.5 35.0 2.0 35.0 96-97 26.366646407481582 34.0 24.5 35.0 2.0 35.0 98-99 25.83912337199197 34.0 21.5 35.0 2.0 35.0 100 24.95332961765746 33.0 17.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06501437897657425 1101 2 -0.09208575548538533 1101 3 -0.05694564830525195 1101 4 -0.048797071496366584 1101 5 -0.037055927247521936 1101 6 -0.04899247166340359 1101 7 -0.041750067749688924 1101 8 -0.025968677187606204 1101 9 -0.051360520208540095 1101 10-11 -0.05684562079270705 1101 12-13 -0.044059853386585246 1101 14-15 -0.11130882737893444 1101 16-17 -0.1509220072108448 1101 18-19 -0.09054513386308116 1101 20-21 -0.09160506612565911 1101 22-23 -0.11439703543345559 1101 24-25 -0.1472852055372087 1101 26-27 -0.10426335799542841 1101 28-29 -0.07126972278979338 1101 30-31 -0.10420311656410775 1101 32-33 -0.1258577402118135 1101 34-35 -0.13435294829151445 1101 36-37 -0.1929729147629473 1101 38-39 -0.21585780530069343 1101 40-41 -0.2699430421133968 1101 42-43 -0.20718126901135747 1101 44-45 -0.19179417406128607 1101 46-47 -0.17638309498511973 1101 48-49 -0.2402848837828131 1101 50-51 -0.22513184597291058 1101 52-53 -0.19914947068298972 1101 54-55 -0.27022987249601726 1101 56-57 -0.30036130497626345 1101 58-59 -0.3751357171445875 1101 60-61 -0.40537074207831836 1101 62-63 -0.29819596632611933 1101 64-65 -0.3011839883962679 1101 66-67 -0.34291336078968726 1101 68-69 -0.31007831371184835 1101 70-71 -0.3607719475643947 1101 72-73 -0.31048884284172473 1101 74-75 -0.3163152522522381 1101 76-77 -0.3158358787934148 1101 78-79 -0.28634416220272385 1101 80-81 -0.315437280878907 1101 82-83 -0.23079216909779277 1101 84-85 -0.22498877672228446 1101 86-87 -0.29080256278516714 1101 88-89 -0.3468125129762001 1101 90-91 -0.3606063887455342 1101 92-93 -0.40164330501003676 1101 94-95 -0.41812460911529925 1101 96-97 -0.47224347102434194 1101 98-99 -0.4832056679024319 1101 100 -0.4232487696535543 1102 1 -0.07559747306585507 1102 2 -0.035801497823293005 1102 3 -0.05478802375690606 1102 4 -0.021533430688890576 1102 5 0.006041726422260751 1102 6 -0.0262009945170405 1102 7 -4.6268592017639776E-4 1102 8 -0.03631447069376037 1102 9 -0.019197975457387884 1102 10-11 -0.04141694765821313 1102 12-13 -0.05652955035292706 1102 14-15 -0.0719468860621717 1102 16-17 -0.13380181625277032 1102 18-19 -0.12054456046672613 1102 20-21 -0.09461039449188746 1102 22-23 -0.17638197541728573 1102 24-25 -0.07223470986747316 1102 26-27 -0.035292915722578755 1102 28-29 -0.05043206360345209 1102 30-31 -0.09552712699542809 1102 32-33 -0.10382252240020051 1102 34-35 -0.10972256805445113 1102 36-37 -0.12820027635127218 1102 38-39 -0.08985085442044038 1102 40-41 -0.13962286082394826 1102 42-43 -0.1562863794573559 1102 44-45 -0.1991006770060224 1102 46-47 -0.26735554661835437 1102 48-49 -0.1981415443218566 1102 50-51 -0.24113228245496998 1102 52-53 -0.21407675964849204 1102 54-55 -0.2384839594983248 1102 56-57 -0.26065612941406613 1102 58-59 -0.2878705514291511 1102 60-61 -0.330353688660594 1102 62-63 -0.38656469306023666 1102 64-65 -0.36276177513934726 1102 66-67 -0.3175788779509716 1102 68-69 -0.35822157507281105 1102 70-71 -0.3617997766607175 1102 72-73 -0.3188517287585846 1102 74-75 -0.39040911654640453 1102 76-77 -0.43385901527472015 1102 78-79 -0.3936935793724565 1102 80-81 -0.39190723065860666 1102 82-83 -0.3368909486418339 1102 84-85 -0.3294100488813285 1102 86-87 -0.3479318257348787 1102 88-89 -0.36758242084702175 1102 90-91 -0.39104089783921125 1102 92-93 -0.4053651008280603 1102 94-95 -0.41234782847954676 1102 96-97 -0.3857646862541486 1102 98-99 -0.4461129735089351 1102 100 -0.3865298024184227 1103 1 -0.1021377341794718 1103 2 -0.08399361116467219 1103 3 -0.06027500483000381 1103 4 -0.07426304487682245 1103 5 -0.017204582531007873 1103 6 -0.03858590430578346 1103 7 -0.011268969955331443 1103 8 0.032761022920439586 1103 9 -0.016129465143059463 1103 10-11 -0.011097048175322755 1103 12-13 0.005346034294859692 1103 14-15 -0.03761426793713696 1103 16-17 -0.04106935788328059 1103 18-19 0.020939972289333753 1103 20-21 -0.03952640240422767 1103 22-23 -0.09871719946231394 1103 24-25 -0.034958552565733214 1103 26-27 -0.07624909811252678 1103 28-29 -0.01628924590965397 1103 30-31 -0.05598647574530702 1103 32-33 -0.08095293750502464 1103 34-35 -0.007139864577837329 1103 36-37 0.019515241650694293 1103 38-39 0.040684900442876426 1103 40-41 -0.006008104073174536 1103 42-43 0.0018434260245783207 1103 44-45 0.01853277252236296 1103 46-47 -0.0066957006727079715 1103 48-49 0.027270950641963054 1103 50-51 0.010394296223829258 1103 52-53 -0.044583073243018134 1103 54-55 -0.02083538278549213 1103 56-57 -0.09439608904166619 1103 58-59 -0.044296891964862084 1103 60-61 -0.07775688834617256 1103 62-63 -0.037138125960979096 1103 64-65 -0.02828601607151171 1103 66-67 -0.026714636058578378 1103 68-69 -0.05854675804192766 1103 70-71 -0.06451269447511976 1103 72-73 -0.1016881615444305 1103 74-75 -0.0679275615406425 1103 76-77 -0.11546014670257065 1103 78-79 -0.05641775191664067 1103 80-81 -0.11346317980392584 1103 82-83 -0.09350021822314858 1103 84-85 -0.05918855674224943 1103 86-87 -0.08232374874719994 1103 88-89 -0.11574319078532369 1103 90-91 -0.11077616653721378 1103 92-93 -0.11012062762342012 1103 94-95 -0.12377271519283184 1103 96-97 -0.1667365344857039 1103 98-99 -0.18918387145288662 1103 100 -0.15148120217838112 1104 1 0.05656727165979092 1104 2 0.05100369894179835 1104 3 0.036128822270313776 1104 4 -0.007083013657613435 1104 5 0.05555381504947832 1104 6 0.035631388047050905 1104 7 0.03365110067532129 1104 8 0.08650731747219709 1104 9 0.0651533947971501 1104 10-11 0.09214908221719043 1104 12-13 0.050719344715076886 1104 14-15 0.0598283600174625 1104 16-17 0.048476063235924016 1104 18-19 0.10615993762760212 1104 20-21 0.12683623868952765 1104 22-23 0.10347193995140458 1104 24-25 0.06384743793562109 1104 26-27 0.06267561936911648 1104 28-29 0.07221651821384967 1104 30-31 0.07105729286165996 1104 32-33 0.024226415866557716 1104 34-35 0.05273728760576546 1104 36-37 0.04524306275087753 1104 38-39 0.07176172118371227 1104 40-41 0.08542716602019595 1104 42-43 0.09222791295575661 1104 44-45 0.030076535833600815 1104 46-47 0.046207615385959855 1104 48-49 0.040797665488355506 1104 50-51 0.05431277510418653 1104 52-53 0.04080475735847244 1104 54-55 0.019292157114122688 1104 56-57 4.1837129522548366E-4 1104 58-59 -0.01008759234850487 1104 60-61 0.02689916117471114 1104 62-63 0.058791684172135206 1104 64-65 0.04491492009965725 1104 66-67 0.015598081989665502 1104 68-69 -0.07747638352778097 1104 70-71 -0.031448589980787034 1104 72-73 -0.027824426429784666 1104 74-75 -0.007646705887388805 1104 76-77 -0.032152474338747794 1104 78-79 -0.020682033911548103 1104 80-81 -0.024578300184870727 1104 82-83 0.017058389825674425 1104 84-85 -0.012753750169238742 1104 86-87 -0.003272503085753442 1104 88-89 0.0713194808863662 1104 90-91 0.04132058414942463 1104 92-93 0.002537879461698367 1104 94-95 0.018148324681764194 1104 96-97 0.02041676079501542 1104 98-99 0.01547124311852599 1104 100 0.07419905023768436 1105 1 0.04113873359370146 1105 2 0.04712179997394372 1105 3 0.048583802116830554 1105 4 0.06607038648990482 1105 5 0.08093913907524808 1105 6 0.07842177153951013 1105 7 0.10209851505545942 1105 8 0.1242554264666964 1105 9 0.12117002336968596 1105 10-11 0.10936862979304607 1105 12-13 0.09281658933790027 1105 14-15 0.12842696377885687 1105 16-17 0.11594139772520862 1105 18-19 0.055118762390925724 1105 20-21 0.0883526446042211 1105 22-23 0.14620725156370895 1105 24-25 0.15477176656864344 1105 26-27 0.1697589697216273 1105 28-29 0.14766245225220587 1105 30-31 0.15071078917852532 1105 32-33 0.13564003783675815 1105 34-35 0.13222808101195227 1105 36-37 0.059335147356620155 1105 38-39 0.13845379075834074 1105 40-41 0.1459278139280542 1105 42-43 0.18705452187495553 1105 44-45 0.21296381961897737 1105 46-47 0.19893517929894955 1105 48-49 0.20450431939563884 1105 50-51 0.21022919662612338 1105 52-53 0.12831693697256696 1105 54-55 0.12226928367814338 1105 56-57 0.12351441454067213 1105 58-59 0.13622596092054806 1105 60-61 0.14478988440347962 1105 62-63 0.14573156738225634 1105 64-65 0.1656791321195783 1105 66-67 0.16399534203532085 1105 68-69 0.12466849464900776 1105 70-71 0.15083690314529719 1105 72-73 0.07879467606789703 1105 74-75 0.11559308946339897 1105 76-77 0.0735493444572306 1105 78-79 0.08486014965135524 1105 80-81 0.09848879836968649 1105 82-83 0.15055002150972285 1105 84-85 0.07249690498396788 1105 86-87 0.06426754798965817 1105 88-89 0.07962364929674237 1105 90-91 0.081105644690453 1105 92-93 0.09715633231319387 1105 94-95 0.05719027550340172 1105 96-97 0.06576177977560604 1105 98-99 0.017548774723014304 1105 100 0.09066764178099973 1106 1 -0.01968608108557035 1106 2 0.0042947953758414315 1106 3 -0.022160825227736325 1106 4 0.009664146541531693 1106 5 0.04219126040081278 1106 6 0.016665218513779223 1106 7 -7.644135738686941E-4 1106 8 0.07823736630037104 1106 9 0.044794411812283386 1106 10-11 0.0032951596508397074 1106 12-13 0.012427051000031497 1106 14-15 -0.01364023938219816 1106 16-17 0.012223473782555061 1106 18-19 0.056653333044046406 1106 20-21 0.016090923438170535 1106 22-23 0.016502185087389876 1106 24-25 0.023942581393590956 1106 26-27 -0.022674668393548814 1106 28-29 -0.0113250345927014 1106 30-31 0.029065892116527436 1106 32-33 -0.009456098051664696 1106 34-35 0.018211323635561882 1106 36-37 0.034956418512493315 1106 38-39 0.01470771653227132 1106 40-41 0.0639510812360129 1106 42-43 0.02383172482193885 1106 44-45 -0.01962433278998077 1106 46-47 -0.02082206464849179 1106 48-49 -0.00831582483830573 1106 50-51 -0.02132959039628446 1106 52-53 -0.058857845994964464 1106 54-55 -0.0076782929184986415 1106 56-57 -0.07135845333834112 1106 58-59 -0.11312079368758532 1106 60-61 -0.0313794224359043 1106 62-63 -0.014069801928314973 1106 64-65 -0.045246266460473805 1106 66-67 -0.019447897723047447 1106 68-69 -0.05713955227017209 1106 70-71 -0.0680194153720386 1106 72-73 -0.08104315510679427 1106 74-75 -0.0837442857197459 1106 76-77 -0.11134393837903289 1106 78-79 -0.09212081046418419 1106 80-81 -0.026023891303150748 1106 82-83 0.03517312576641629 1106 84-85 0.013628473722548051 1106 86-87 -0.012766996583692958 1106 88-89 0.013899427466185443 1106 90-91 0.005972827660500002 1106 92-93 0.012264955880876727 1106 94-95 -0.01178695764231108 1106 96-97 -0.013552827363234599 1106 98-99 -0.03822316473583598 1106 100 0.010360020981210027 1107 1 -0.056260269518350725 1107 2 -0.02985820277450557 1107 3 -0.039728869588323334 1107 4 -0.0035653823688264197 1107 5 0.006318172329635274 1107 6 -0.009161640784249414 1107 7 0.011465280762600116 1107 8 0.018271632391481774 1107 9 -0.029189006147781527 1107 10-11 -0.053409219154460175 1107 12-13 -0.02844741757409963 1107 14-15 -0.010318689251221258 1107 16-17 -0.013745636339997702 1107 18-19 -0.05427446702891103 1107 20-21 -0.032123734853811925 1107 22-23 -0.03174246286265259 1107 24-25 -0.05849003232094674 1107 26-27 -0.02869010391447091 1107 28-29 -0.00983979169949123 1107 30-31 6.525120173961341E-4 1107 32-33 -0.046674844795525416 1107 34-35 -0.012837462343952666 1107 36-37 -0.007946959280566546 1107 38-39 -0.023832409393527598 1107 40-41 0.005351857423811168 1107 42-43 -0.08593684285104075 1107 44-45 -0.09747341274396604 1107 46-47 -0.19370702514495264 1107 48-49 -0.0705580757364146 1107 50-51 -0.05131238259158977 1107 52-53 -0.025227505781330706 1107 54-55 -0.11090463907626713 1107 56-57 -0.08015591366866204 1107 58-59 -0.11964265161136467 1107 60-61 -0.12127201171737312 1107 62-63 -0.14697550067240428 1107 64-65 -0.06631540860514207 1107 66-67 -0.05149381205401227 1107 68-69 -0.08313759885221117 1107 70-71 -0.05388475896580758 1107 72-73 -0.06291993102627558 1107 74-75 -0.04401380768531027 1107 76-77 -0.0213464728783741 1107 78-79 0.028946312541251018 1107 80-81 -0.04746123603311858 1107 82-83 0.05018051632890419 1107 84-85 0.045603655589101066 1107 86-87 0.07051453266503671 1107 88-89 0.10626653633578798 1107 90-91 0.08429050038387942 1107 92-93 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2208 76-77 0.09150133241977798 2208 78-79 0.0943530758519664 2208 80-81 0.14641420501533275 2208 82-83 0.13998621472978456 2208 84-85 0.17895754970327005 2208 86-87 0.20677073124214118 2208 88-89 0.25358767478630995 2208 90-91 0.32319929414026305 2208 92-93 0.3930047208895857 2208 94-95 0.432694279506471 2208 96-97 0.4907230650804024 2208 98-99 0.685010649275501 2208 100 0.6087866649148133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 373764.0 3 124977.0 4 77030.0 5 65139.0 6 63506.0 7 72004.0 8 79029.0 9 85158.0 10 90665.0 11 94645.0 12 98602.0 13 124039.0 14 186849.0 15 272409.0 16 341152.0 17 289557.0 18 264844.0 19 222608.0 20 197694.0 21 197136.0 22 207576.0 23 225844.0 24 250747.0 25 284909.0 26 325132.0 27 373900.0 28 435448.0 29 506098.0 30 601560.0 31 732279.0 32 861759.0 33 1056615.0 34 1410315.0 35 2009445.0 36 3182788.0 37 5586797.0 38 7621014.0 39 2649146.0 40 9308.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.976858395935164 28.45531941856145 26.949511166735146 22.618311018768235 2 24.43657659378003 27.70124349757539 27.307747403974723 20.554432504669855 3 23.83446765592968 26.536485437287066 26.449366277144332 23.179680629638927 4 24.532709822693768 25.689264978014837 26.512252893994447 23.265772305296945 5 25.067819900983768 25.93780503312718 25.67193559975659 23.322439466132458 6 25.19940505661435 25.684431659648 25.416685650703375 23.699477633034284 7 24.82152895892759 26.31916802506246 25.244820119031147 23.614482896978803 8 24.410561422243436 25.77990338937906 25.90412760815323 23.90540758022427 9 24.342101333861866 25.854886292667683 25.727424919057206 24.075587454413245 10-11 24.477054746245816 25.828638002474886 25.744177575677696 23.950129675601598 12-13 23.950647842135755 25.941249584140362 25.999842368679627 24.108260205044253 14-15 23.490758404547314 26.158571261717977 26.69064416049583 23.66002617323888 16-17 23.235111088284725 26.11751051188446 27.03003420160887 23.617344198221936 18-19 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29.37641779351539 22.03716604565055 44-45 22.45008819061085 26.52526623278798 28.889999089140733 22.134646487460437 46-47 22.6008794581964 26.783690480559248 28.406312069004763 22.209117992239587 48-49 22.70497318919823 26.915752131170912 27.950515180149704 22.42875949948115 50-51 22.73218838416194 27.11341715912082 27.652545270173672 22.501849186543563 52-53 22.81486461526582 27.28612505442997 27.406910456057897 22.492099874246314 54-55 22.858424553328856 27.319313131805878 27.251293460601637 22.57096885426363 56-57 22.84188339718031 27.478981856285532 27.12570771851518 22.553427028018973 58-59 22.898997431168866 27.622806415112162 27.00152804035977 22.476668113359207 60-61 22.86529476544354 27.71405446315174 26.86670361475577 22.55394715664895 62-63 22.86372670160221 27.885110564573353 26.779512225423517 22.47165050840092 64-65 22.90931954526106 28.06715537875445 26.617322017033096 22.406203058951395 66-67 22.91190010657652 28.1556748052523 26.478359623004017 22.454065465167158 68-69 22.913110448271087 28.323622155233956 26.337192753355136 22.426074643139817 70-71 22.94146498382433 28.490654370824696 26.19885738833488 22.369023257016092 72-73 22.922276521139132 28.572835960211556 26.048188567636476 22.456698951012836 74-75 22.954168024301605 28.718110691576946 25.959288549899924 22.36843273422153 76-77 22.826284595039336 28.99576691194993 25.738727275559302 22.439221217451426 78-79 22.856057817021817 29.08497007186055 25.602121497164447 22.456850613953186 80-81 22.84611708652542 29.2384849856361 25.48170527977024 22.43369264806824 82-83 22.860939162413914 29.41758534267793 25.334534451041552 22.3869410438666 84-85 22.842155747213816 29.498789992400486 25.179956086354654 22.479098174031044 86-87 22.83498502170875 29.645435364438683 25.0432944386121 22.476285175240463 88-89 22.926867167574542 29.770979764358913 24.83591784113225 22.466235226934295 90-91 22.869784749848684 29.865710869942912 24.66978653812086 22.59471784208754 92-93 22.902942706829236 29.980384292571326 24.5439268243085 22.572746176290938 94-95 22.968896517338287 30.11870368366681 24.345497667460364 22.566902131534544 96-97 22.989061741331827 30.147524925965953 24.155684205306027 22.707729127396192 98-99 22.984086279072038 30.2603874699224 24.005167702280993 22.750358548724567 100 23.302355125910694 30.207526573524657 23.435321096815535 23.054797203749107 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 25990.0 1 15351.5 2 4070.0 3 3347.5 4 3430.0 5 3532.0 6 3600.0 7 3924.5 8 4511.5 9 5312.5 10 6463.5 11 8284.0 12 11721.5 13 20009.5 14 38617.0 15 58318.5 16 101628.0 17 112605.0 18 88845.5 19 92563.0 20 104536.0 21 104733.0 22 97013.0 23 105089.0 24 121075.0 25 143452.0 26 170627.0 27 201704.5 28 239848.0 29 279924.0 30 317927.5 31 361442.5 32 415284.0 33 476578.5 34 541989.0 35 619718.5 36 707941.0 37 799935.0 38 890010.0 39 959685.0 40 1010844.5 41 1062461.5 42 1122385.0 43 1184000.5 44 1239057.0 45 1268934.5 46 1263819.5 47 1233981.5 48 1190309.5 49 1152008.5 50 1111835.0 51 1061236.5 52 1000440.5 53 932074.0 54 867523.5 55 807845.0 56 738652.0 57 669815.0 58 611849.0 59 556275.0 60 499943.5 61 445241.5 62 391938.5 63 337726.5 64 290365.0 65 247214.5 66 209128.0 67 174799.0 68 143034.5 69 116553.5 70 95005.5 71 77323.0 72 63107.0 73 51423.5 74 41188.5 75 31738.0 76 24033.0 77 18963.0 78 15280.5 79 11435.5 80 7658.5 81 4940.0 82 3309.5 83 2261.0 84 1581.0 85 1115.5 86 768.5 87 508.0 88 340.5 89 223.5 90 120.5 91 68.0 92 43.5 93 33.5 94 30.0 95 21.0 96 11.5 97 8.0 98 5.5 99 4.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1745036497021451 2 0.0018988049439825686 3 0.0018482543963890229 4 0.0018861673070841823 5 0.023325918305196845 6 0.006600005870182339 7 0.014141515689294473 8 0.017326200187687865 9 0.020871057337685274 10-11 0.0036696538143689743 12-13 0.005325184248057603 14-15 0.021961053520171105 16-17 0.0061687465110249005 18-19 0.008934809287159241 20-21 0.020523522322979645 22-23 0.017561576174920313 24-25 0.010985265873922448 26-27 0.03351817246374554 28-29 0.019692597696910733 30-31 0.018166603041430564 32-33 0.026167806902721504 34-35 0.038123011408595114 36-37 0.003639639426735306 38-39 0.011219062156542598 40-41 0.015566409249587548 42-43 0.013143142374321938 44-45 0.01751734444577596 46-47 0.0030883225170431957 48-49 0.0025622808811478592 50-51 0.014102023073987014 52-53 0.0022905716878325495 54-55 0.0011026338193842204 56-57 0.006276166424661186 58-59 0.010854150391101687 60-61 0.013849270336019282 62-63 0.006805367469781119 64-65 0.011836726659951237 66-67 0.0075715242067458 68-69 0.009454532104605387 70-71 0.0076268138681762415 72-73 0.006938062657214177 74-75 7.708958508015752E-4 76-77 0.0018893267163087788 78-79 8.040696476598399E-4 80-81 0.0030393516740619487 82-83 0.0013016766005338075 84-85 0.006631599962428306 86-87 0.004258883634756244 88-89 0.004704360335424367 90-91 0.002603353201067615 92-93 0.005848066474728344 94-95 0.004873388728940286 96-97 0.011741944383213338 98-99 0.01189833513983087 100 0.011184308655072035 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.1651487E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.26387929277023 #Duplication Level Percentage of deduplicated Percentage of total 1 75.39307300423478 36.38762174987401 2 13.403733979501425 12.938323977181193 3 4.524328710882776 6.55084964348583 4 2.060003936989486 3.97695125429968 5 1.1532523396198133 2.783021585675777 6 0.7239510337963291 2.0964411185413345 7 0.4638089301840185 1.5669652754917256 8 0.3262417158647568 1.259655263581029 9 0.25583285619967533 1.1112737481671195 >10 1.4152362505526246 13.058858746135224 >50 0.150503069331261 5.081247313473957 >100 0.1286819811651505 10.117098136549304 >500 7.547551103557914E-4 0.2505777406435377 >1k 4.274559105340156E-4 0.415467785803394 >5k 3.268858802268956E-5 0.10028983928276919 >10k+ 1.372920690923726E-4 2.3053568218142395 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 128947 0.4073963412840604 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 86149 0.27217994528977424 No Hit ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 78358 0.24756498802094196 No Hit GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59629 0.18839241265347187 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45270 0.143026455597489 No Hit ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36449 0.11515730682732221 No Hit TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32037 0.10121799332840191 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 9.47822767378986E-6 0.0 1.895645534757972E-5 0.0 2 0.0 2.527527379677296E-5 0.0 2.527527379677296E-5 0.0 3 0.0 3.475350147056282E-5 0.0 2.843468302136958E-5 9.47822767378986E-6 4 0.0 4.107231991975606E-5 0.0 3.15940922459662E-5 9.47822767378986E-6 5 3.15940922459662E-6 4.423172914435268E-5 0.0 3.791291069515944E-5 9.47822767378986E-6 6 6.31881844919324E-6 5.370995681814254E-5 0.0 4.423172914435268E-5 9.47822767378986E-6 7 1.57970461229831E-5 5.370995681814254E-5 0.0 4.73911383689493E-5 9.47822767378986E-6 8 1.895645534757972E-5 6.0028775267335784E-5 0.0 4.73911383689493E-5 9.47822767378986E-6 9 3.15940922459662E-5 6.31881844919324E-5 0.0 5.055054759354592E-5 9.47822767378986E-6 10-11 6.160847987963409E-5 7.582582139031888E-5 0.0 5.528966143044085E-5 9.47822767378986E-6 12-13 1.7850662118970903E-4 8.846345828870536E-5 1.57970461229831E-6 6.160847987963409E-5 9.47822767378986E-6 14-15 5.292010451199339E-4 9.320257212560029E-5 3.15940922459662E-6 6.634759371652902E-5 1.105793228608817E-5 16-17 0.0012542854621648582 1.0741991363628509E-4 3.15940922459662E-6 7.266641216572226E-5 1.263763689838648E-5 18-19 0.0023837742599581496 1.1689814131007495E-4 3.15940922459662E-6 8.214463983951212E-5 1.4217341510684789E-5 20-21 0.003919247143112107 1.3585459665765466E-4 3.15940922459662E-6 9.320257212560029E-5 2.369556918447465E-5 22-23 0.006184543557147884 1.437531197191462E-4 3.15940922459662E-6 1.0741991363628509E-4 2.685497840907127E-5 24-25 0.009212837298923745 1.721878027405158E-4 3.15940922459662E-6 1.1373873208547833E-4 3.001438763366789E-5 26-27 0.01262815867071269 1.9588337192499045E-4 3.15940922459662E-6 1.2321695975926817E-4 3.15940922459662E-5 28-29 0.017052911289760258 2.0694130421107862E-4 3.15940922459662E-6 1.2953577820846142E-4 3.15940922459662E-5 30-31 0.02050614557224436 2.2747746417095665E-4 3.15940922459662E-6 1.3743430126995297E-4 3.791291069515944E-5 32-33 0.022553442749782972 2.4801362413083463E-4 3.15940922459662E-6 1.5323134739293607E-4 3.791291069515944E-5 34-35 0.024618116678056863 2.6697007947841436E-4 3.15940922459662E-6 1.6270957506672593E-4 3.791291069515944E-5 36-37 0.02688341309209264 2.8908594405059075E-4 3.15940922459662E-6 1.7534721196511241E-4 4.265202453205437E-5 38-39 0.02906182575245201 3.0172358094897724E-4 3.15940922459662E-6 1.8008632580200734E-4 4.423172914435268E-5 40-41 0.031098064997704532 3.080423993981705E-4 3.15940922459662E-6 1.895645534757972E-4 4.423172914435268E-5 42-43 0.03345656398386591 3.1120180862276704E-4 3.15940922459662E-6 1.9904278114958707E-4 4.897084298124761E-5 44-45 0.03607413452644421 3.175206270719603E-4 3.15940922459662E-6 2.03781894986482E-4 5.055054759354592E-5 46-47 0.03921458729569324 3.3331767319494344E-4 3.15940922459662E-6 2.1168041804797355E-4 5.370995681814254E-5 48-49 0.042996400137535404 3.601726516040147E-4 3.15940922459662E-6 2.211586457217634E-4 5.370995681814254E-5 50-51 0.04742747157503216 3.712305838901029E-4 3.15940922459662E-6 2.353759872324482E-4 5.528966143044085E-5 52-53 0.052994350628771406 3.933464484622792E-4 3.15940922459662E-6 2.4959332874313297E-4 5.686936604273916E-5 54-55 0.06020570218391319 4.028246761360691E-4 3.15940922459662E-6 2.559121471923262E-4 5.686936604273916E-5 56-57 0.0692668878400563 4.123029038098589E-4 3.15940922459662E-6 2.638106702538178E-4 6.31881844919324E-5 58-59 0.08077819534987407 4.202014268713505E-4 3.15940922459662E-6 2.685497840907127E-4 6.31881844919324E-5 60-61 0.09466063948275164 4.359984729943336E-4 3.15940922459662E-6 2.859265348259941E-4 6.31881844919324E-5 62-63 0.11167721756642904 4.438969960558251E-4 3.15940922459662E-6 3.048829901735738E-4 6.950700294112564E-5 64-65 0.1326288398393415 4.53375223729615E-4 3.15940922459662E-6 3.191003316842586E-4 7.108670755342395E-5 66-67 0.15856285045944288 4.6285345140340483E-4 3.15940922459662E-6 3.3015826397034677E-4 7.266641216572226E-5 68-69 0.19025014527753467 4.7707079291408965E-4 6.31881844919324E-6 3.3805678703183835E-4 7.266641216572226E-5 70-71 0.2293952255702868 4.991866574862659E-4 6.31881844919324E-6 3.459553100933299E-4 7.266641216572226E-5 72-73 0.2763977566046107 5.118242943846524E-4 6.31881844919324E-6 3.5069442393022483E-4 7.266641216572226E-5 74-75 0.33265103784855354 5.213025220584423E-4 6.31881844919324E-6 3.5701324237941807E-4 7.266641216572226E-5 76-77 0.4005656985404825 5.355198635691272E-4 6.31881844919324E-6 3.6649147005320793E-4 7.266641216572226E-5 78-79 0.479969550877657 5.465777958552153E-4 6.31881844919324E-6 3.6649147005320793E-4 7.266641216572226E-5 80-81 0.5723380389679638 5.607951373659001E-4 6.31881844919324E-6 3.7438999311469945E-4 7.266641216572226E-5 82-83 0.6799348795208263 5.702733650396899E-4 7.89852306149155E-6 3.8702763001308594E-4 7.424611677802057E-5 84-85 0.8041091402751472 5.750124788765848E-4 9.47822767378986E-6 3.933464484622792E-4 7.582582139031888E-5 86-87 0.9463252074065271 5.813312973257781E-4 9.47822767378986E-6 3.949261530745775E-4 7.582582139031888E-5 88 1.064784096873553 5.90809524999568E-4 9.47822767378986E-6 3.9808556229917414E-4 7.582582139031888E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCTCG 24145 0.0 55.5024 2 ATATCTC 28235 0.0 47.7537 1 ATCTCGA 36145 0.0 37.03686 3 TATGGAT 30310 0.0 36.955566 2 ATATGGA 31200 0.0 36.168896 1 TCTCGAG 40975 0.0 32.464848 4 ATGGATC 36430 0.0 30.631197 3 GGATCCG 38110 0.0 28.874182 5 CTCGAGG 46210 0.0 28.502266 5 TGGATCC 43150 0.0 25.817453 4 GATCCGG 55725 0.0 24.804346 1 TCGAGGG 54805 0.0 23.908363 6 ATCCGGC 61680 0.0 22.21447 2 TCCGGCG 72475 0.0 19.022444 3 CGAGGGC 72660 0.0 18.11138 7 GCGCCGT 82165 0.0 16.794579 9 CGGCGCG 82850 0.0 16.600801 5 GAGGGCG 80630 0.0 16.286287 8 AGGGCGC 81785 0.0 15.97611 9 CCGGCGC 88520 0.0 15.611758 4 >>END_MODULE