FastQCFastQC Report
Wed 25 May 2016
SRR490962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45583212
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2239160.49122470790342726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG178350.041.061582
ATCTCGA298750.032.9331741
TCTCGAG351800.027.8714642
ATATCTC271450.027.782281
CTCGAGG427800.022.8433273
GATCCGG565050.022.2943331
TATGGAT328450.022.2537122
ATCCGGC600350.020.881412
ATATGGA369300.020.127881
TCGAGGG509150.019.1658124
ATGGATC394050.018.5849653
GGATCCG387950.018.380465
TCCGGCG698050.018.0667383
GCGCCGT749700.016.8074579
CGGCGCG790300.015.9221965
TGGATCC460200.015.7807444
AGGGCGC764550.015.1487272
CGTCGAC712600.014.70423612-13
GAGGGCG800100.014.6445441
CCGGCGC878500.014.409214
Produced by FastQC (version 0.11.5)